American Journal of Health Research

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Isolation and Antimicrobial Sensitivity Patterns of Enteric Bacterial Pathogens from Asymptomatic Food Handlers, Jimma, Ethiopia

Received: 12 October 2015    Accepted: 26 October 2015    Published: 04 January 2016
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Abstract

Background: Most enteric bacteria are harmless but species which belong to Salmonella, Shigella, Yersinia and certain strains of Escherichia coli are pathogens. The emergences of increased antimicrobial resistances are global challenges, particularly in developing countries due to misuse of antimicrobial agents. This study was aimed at determining the prevalence and antimicrobial sensitivity patterns of enteric bacterial pathogens isolated from food handlers in Jimma Town, Ethiopia. Methods: A cross-sectional study was conducted from July 16/2012 to November 23/2012 among 218 food handlers. Structured questionnaire was used to assess associated factors for enteric bacterial infection. Stool samples were collected andantimicrobial sensitivity tests were done using Mueller Hinton Agar (MHA) by Kirby-Bauer disc diffusion method following standard procedures. Descriptive statistics were performed using SPSS version 16.P-values less than 0.05 were considered as statistically significant. Results: The prevalence of enteric bacterial pathogens was found to be 6.9% (15/218). All S. typhi isolates showed resistance to two antimicrobials; amoxicillin-clavulanic acid and streptomycin. However, they all showed sensitivity to most antimicrobials. Half of Shigella isolates showed resistance to two antimicrobials; chloramphenicol and trimethoprim-sulfamethoxazole where as the other half showed resistant toother four antimicrobials; amoxicillin-clavulanic acid, ampicillin, streptomycin and tetracycline. One-third of enterohaemorrhagic Escherichia coli (EHEC) O157:H7 strains of the isolates were found to be resistant to two antimicrobials; amoxicillin-clavulanic acid and tetracycline. Enteric bacterial infection had a significant association with poor training in food handling and preparation (p=0.034), not knowledgeable about food borne infection (p<0.001) and inconsistent hand washing (p=0.033). Conclusion: Most of the isolates were resistant to ampicillin, streptomycin and tetracycline while all were sensitive to ciprofloxacin and nalidixic acid. Health education along with continuous food safety training should be given to food handlers so that they can adhere with effective hygienic practices.

DOI 10.11648/j.ajhr.20150306.24
Published in American Journal of Health Research (Volume 3, Issue 6, November 2015)
Page(s) 399-406
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Antimicrobial, Enteric Bacterial Pathogens, Food Handler, Sensitivity

References
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Author Information
  • Department of Biomedical Science, College of Health Sciences, Mizan Tepi University, MizanTeferi, Ethiopia

  • Department of Biomedical Science, College of Health Sciences, Mizan Tepi University, MizanTeferi, Ethiopia

  • Departmentof Medical Laboratory Sciences, College of Health and Medical Sciences, Jimma University, Jimma, Ethiopia

  • Departmentof Medical Laboratory Sciences, College of Health and Medical Sciences, Jimma University, Jimma, Ethiopia

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    Teshale Worku, Ayalew Jejaw, Subbaram Kannan, Beyene Wondafrash. (2016). Isolation and Antimicrobial Sensitivity Patterns of Enteric Bacterial Pathogens from Asymptomatic Food Handlers, Jimma, Ethiopia. American Journal of Health Research, 3(6), 399-406. https://doi.org/10.11648/j.ajhr.20150306.24

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    ACS Style

    Teshale Worku; Ayalew Jejaw; Subbaram Kannan; Beyene Wondafrash. Isolation and Antimicrobial Sensitivity Patterns of Enteric Bacterial Pathogens from Asymptomatic Food Handlers, Jimma, Ethiopia. Am. J. Health Res. 2016, 3(6), 399-406. doi: 10.11648/j.ajhr.20150306.24

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    AMA Style

    Teshale Worku, Ayalew Jejaw, Subbaram Kannan, Beyene Wondafrash. Isolation and Antimicrobial Sensitivity Patterns of Enteric Bacterial Pathogens from Asymptomatic Food Handlers, Jimma, Ethiopia. Am J Health Res. 2016;3(6):399-406. doi: 10.11648/j.ajhr.20150306.24

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  • @article{10.11648/j.ajhr.20150306.24,
      author = {Teshale Worku and Ayalew Jejaw and Subbaram Kannan and Beyene Wondafrash},
      title = {Isolation and Antimicrobial Sensitivity Patterns of Enteric Bacterial Pathogens from Asymptomatic Food Handlers, Jimma, Ethiopia},
      journal = {American Journal of Health Research},
      volume = {3},
      number = {6},
      pages = {399-406},
      doi = {10.11648/j.ajhr.20150306.24},
      url = {https://doi.org/10.11648/j.ajhr.20150306.24},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajhr.20150306.24},
      abstract = {Background: Most enteric bacteria are harmless but species which belong to Salmonella, Shigella, Yersinia and certain strains of Escherichia coli are pathogens. The emergences of increased antimicrobial resistances are global challenges, particularly in developing countries due to misuse of antimicrobial agents. This study was aimed at determining the prevalence and antimicrobial sensitivity patterns of enteric bacterial pathogens isolated from food handlers in Jimma Town, Ethiopia. Methods: A cross-sectional study was conducted from July 16/2012 to November 23/2012 among 218 food handlers. Structured questionnaire was used to assess associated factors for enteric bacterial infection. Stool samples were collected andantimicrobial sensitivity tests were done using Mueller Hinton Agar (MHA) by Kirby-Bauer disc diffusion method following standard procedures. Descriptive statistics were performed using SPSS version 16.P-values less than 0.05 were considered as statistically significant. Results: The prevalence of enteric bacterial pathogens was found to be 6.9% (15/218). All S. typhi isolates showed resistance to two antimicrobials; amoxicillin-clavulanic acid and streptomycin. However, they all showed sensitivity to most antimicrobials. Half of Shigella isolates showed resistance to two antimicrobials; chloramphenicol and trimethoprim-sulfamethoxazole where as the other half showed resistant toother four antimicrobials; amoxicillin-clavulanic acid, ampicillin, streptomycin and tetracycline. One-third of enterohaemorrhagic Escherichia coli (EHEC) O157:H7 strains of the isolates were found to be resistant to two antimicrobials; amoxicillin-clavulanic acid and tetracycline. Enteric bacterial infection had a significant association with poor training in food handling and preparation (p=0.034), not knowledgeable about food borne infection (p<0.001) and inconsistent hand washing (p=0.033). Conclusion: Most of the isolates were resistant to ampicillin, streptomycin and tetracycline while all were sensitive to ciprofloxacin and nalidixic acid. Health education along with continuous food safety training should be given to food handlers so that they can adhere with effective hygienic practices.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Isolation and Antimicrobial Sensitivity Patterns of Enteric Bacterial Pathogens from Asymptomatic Food Handlers, Jimma, Ethiopia
    AU  - Teshale Worku
    AU  - Ayalew Jejaw
    AU  - Subbaram Kannan
    AU  - Beyene Wondafrash
    Y1  - 2016/01/04
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ajhr.20150306.24
    DO  - 10.11648/j.ajhr.20150306.24
    T2  - American Journal of Health Research
    JF  - American Journal of Health Research
    JO  - American Journal of Health Research
    SP  - 399
    EP  - 406
    PB  - Science Publishing Group
    SN  - 2330-8796
    UR  - https://doi.org/10.11648/j.ajhr.20150306.24
    AB  - Background: Most enteric bacteria are harmless but species which belong to Salmonella, Shigella, Yersinia and certain strains of Escherichia coli are pathogens. The emergences of increased antimicrobial resistances are global challenges, particularly in developing countries due to misuse of antimicrobial agents. This study was aimed at determining the prevalence and antimicrobial sensitivity patterns of enteric bacterial pathogens isolated from food handlers in Jimma Town, Ethiopia. Methods: A cross-sectional study was conducted from July 16/2012 to November 23/2012 among 218 food handlers. Structured questionnaire was used to assess associated factors for enteric bacterial infection. Stool samples were collected andantimicrobial sensitivity tests were done using Mueller Hinton Agar (MHA) by Kirby-Bauer disc diffusion method following standard procedures. Descriptive statistics were performed using SPSS version 16.P-values less than 0.05 were considered as statistically significant. Results: The prevalence of enteric bacterial pathogens was found to be 6.9% (15/218). All S. typhi isolates showed resistance to two antimicrobials; amoxicillin-clavulanic acid and streptomycin. However, they all showed sensitivity to most antimicrobials. Half of Shigella isolates showed resistance to two antimicrobials; chloramphenicol and trimethoprim-sulfamethoxazole where as the other half showed resistant toother four antimicrobials; amoxicillin-clavulanic acid, ampicillin, streptomycin and tetracycline. One-third of enterohaemorrhagic Escherichia coli (EHEC) O157:H7 strains of the isolates were found to be resistant to two antimicrobials; amoxicillin-clavulanic acid and tetracycline. Enteric bacterial infection had a significant association with poor training in food handling and preparation (p=0.034), not knowledgeable about food borne infection (p<0.001) and inconsistent hand washing (p=0.033). Conclusion: Most of the isolates were resistant to ampicillin, streptomycin and tetracycline while all were sensitive to ciprofloxacin and nalidixic acid. Health education along with continuous food safety training should be given to food handlers so that they can adhere with effective hygienic practices.
    VL  - 3
    IS  - 6
    ER  - 

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