Isolation and Identification of <i>Enterobacteriaceae</i> from Patients with Community Acquired Urinary Tract Infection

Abd Elrahman Mustafa Abd Elrahman Osman; Shingray Osman Hashim; Mohammed Abdall Musa; Omer Mohammed Tahir

doi:doi:10.11648/j.ajhr.20180601.14

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Isolation and Identification of Enterobacteriaceae from Patients with Community Acquired Urinary Tract Infection

Abd Elrahman Mustafa Abd Elrahman Osman, Shingray Osman Hashim, Mohammed Abdall Musa, Omer Mohammed Tahir

Published in American Journal of Health Research (Volume 6, Issue 1)

Received: 28 October 2017 Accepted: 4 December 2017 Published: 22 January 2018

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Abstract

The study was carried out in red sea state during the period from November 2013 to March 2014 to investigate the Enterobacteriaceae from patients suffering from community-acquired urinary tract infections and then do sensitivity test for each of isolates. In this study out of 100 specimens 52 Enterobacteriaceae species were isolated from different clinics and hospitals in Port Sudan city. The specimens werecultured on CLED media ( cystinelactose electrolyte deficiency). identification was done by gram's stain and convential biochemical reactions. Then the anti–microbial sensitivity tests were done as the follows: (Ampicillin-Sulbactam "AS", Co. trimoxazole "BA", Ceftizoxime "CI", Chloramphenicol "CH", Cephalaxin "PR", Tetracycline "TE", Ciprofloxacin "CP", Amikacin "AK", Sparfloxacin "SC", Gatifloxacin "GF", Norflaxcin "NX) by Kirby-Bauer disc diffusion method. The study revealed that the most part of strains are sensitive to Chloramphenicol andAmikacin and resistant to Ampicillin –Sulpactam (AS). The identified Enterobacteriaceae were as follows; Escherichia coli 34 (65%), Klebsiella pneumonia 10 (19%), Klebsiella oxytoca 3(6%), Salmonella Para A 3 (6%), proteus mirabilis 1 (2%), Citrobacter 1 (2%).

Published in	American Journal of Health Research (Volume 6, Issue 1)
DOI	10.11648/j.ajhr.20180601.14
Page(s)	25-31
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Enterobacteriaceae, Urinary Tract Infections, Escherichia coli, Red SeaState

References

[1]	Okesola. A. O and Makanjuola. O. (2009). Resistance to Third -generation Cephalosporins and other Antibiotics by Enterobactericeae in Western Nigeria, American Journal of Infection disease, 5(1):17-20.
[2]	Murray Patrick. R, Rosenthal. Kens, Kabayashi George S and Pfaaller. Michael A. (2002). Medical Microbiology, 4th edition, Mosby. P266-279.
[3]	Rayan. Kenneth. J and George. C. Ray. (2004). Sherris Medical Microbiology4 th edition, McGraw -HILL, New York, Chicago. P. 343-367.
[4]	Panahi Y, Beiraghdar F, Moharamzad Y, Matinzadeh ZK, EinollahiB. The incidence of urinary tract infections in febrile children during a two-year period in Tehran, Iran. Trop Doct. 2008; 38(4):247-9.
[5]	Sharifian M, Karimi A, Tabatabaei SR, Anvaripour N. Microbial sensitivity pattern in urinary tract infections in children: a single center experience of 1,177 urine cultures. Jpn J Infect Dis. 2006;59(6):380-2.
[6]	Orret and Shurland, 1998. Bacterial etiologic agents of urinry tract infection in Hospitalized patients Nigeria, Abuja.
[7]	Modarres S, Oskoii NN. Bacterial etiologic agents of urinary tract infection in children in the Islamic Republic of Iran. East MediterrHealth. 1997;3:290-5.
[8]	Gupta K. Emerging antibiotic resistance in urinary tract pathogens Infect Dis Clin North Am. 2003;17(2):243-59.
[9]	Torkaman M, Afsharpaiman SH, Hoseini MJ, Moradi M, Mazraati A, Amirsalari S, et al. Platelet count and neonatal sepsis: a high prevalence of Enterobacter spp. Singapore Med J. 2009; 50(5):482-512
[10]	Collee, J. G; Morinon, B. P.; Fraser, A. G. Simmons, A. (1996). Maki and McCartney Practical Medical Microbiology (14th edition). Churchill Livingstone New York.

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APA Style

Abd Elrahman Mustafa Abd Elrahman Osman, Shingray Osman Hashim, Mohammed Abdall Musa, Omer Mohammed Tahir. (2018). Isolation and Identification of Enterobacteriaceae from Patients with Community Acquired Urinary Tract Infection. American Journal of Health Research, 6(1), 25-31. https://doi.org/10.11648/j.ajhr.20180601.14

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ACS Style

Abd Elrahman Mustafa Abd Elrahman Osman; Shingray Osman Hashim; Mohammed Abdall Musa; Omer Mohammed Tahir. Isolation and Identification of Enterobacteriaceae from Patients with Community Acquired Urinary Tract Infection. Am. J. Health Res. 2018, 6(1), 25-31. doi: 10.11648/j.ajhr.20180601.14

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AMA Style

Abd Elrahman Mustafa Abd Elrahman Osman, Shingray Osman Hashim, Mohammed Abdall Musa, Omer Mohammed Tahir. Isolation and Identification of Enterobacteriaceae from Patients with Community Acquired Urinary Tract Infection. Am J Health Res. 2018;6(1):25-31. doi: 10.11648/j.ajhr.20180601.14

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@article{10.11648/j.ajhr.20180601.14,
  author = {Abd Elrahman Mustafa Abd Elrahman Osman and Shingray Osman Hashim and Mohammed Abdall Musa and Omer Mohammed Tahir},
  title = {Isolation and Identification of Enterobacteriaceae from Patients with Community Acquired Urinary Tract Infection},
  journal = {American Journal of Health Research},
  volume = {6},
  number = {1},
  pages = {25-31},
  doi = {10.11648/j.ajhr.20180601.14},
  url = {https://doi.org/10.11648/j.ajhr.20180601.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20180601.14},
  abstract = {The study was carried out in red sea state during the period from November 2013 to March 2014 to investigate the Enterobacteriaceae from patients suffering from community-acquired urinary tract infections and then do sensitivity test for each of isolates. In this study out of 100 specimens 52 Enterobacteriaceae species were isolated from different clinics and hospitals in Port Sudan city. The specimens werecultured on CLED media ( cystinelactose electrolyte deficiency). identification was done by gram's stain and convential biochemical reactions. Then the anti–microbial sensitivity tests were done as the follows: (Ampicillin-Sulbactam "AS", Co. trimoxazole "BA", Ceftizoxime "CI", Chloramphenicol "CH", Cephalaxin "PR", Tetracycline "TE", Ciprofloxacin "CP", Amikacin "AK", Sparfloxacin "SC", Gatifloxacin "GF", Norflaxcin "NX) by Kirby-Bauer disc diffusion method. The study revealed that the most part of strains are sensitive to Chloramphenicol andAmikacin and resistant to Ampicillin –Sulpactam (AS). The identified Enterobacteriaceae were as follows; Escherichia coli 34 (65%), Klebsiella pneumonia 10 (19%), Klebsiella oxytoca 3(6%), Salmonella Para A 3 (6%), proteus mirabilis 1 (2%), Citrobacter 1 (2%).},
 year = {2018}
}

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TY  - JOUR
T1  - Isolation and Identification of Enterobacteriaceae from Patients with Community Acquired Urinary Tract Infection
AU  - Abd Elrahman Mustafa Abd Elrahman Osman
AU  - Shingray Osman Hashim
AU  - Mohammed Abdall Musa
AU  - Omer Mohammed Tahir
Y1  - 2018/01/22
PY  - 2018
N1  - https://doi.org/10.11648/j.ajhr.20180601.14
DO  - 10.11648/j.ajhr.20180601.14
T2  - American Journal of Health Research
JF  - American Journal of Health Research
JO  - American Journal of Health Research
SP  - 25
EP  - 31
PB  - Science Publishing Group
SN  - 2330-8796
UR  - https://doi.org/10.11648/j.ajhr.20180601.14
AB  - The study was carried out in red sea state during the period from November 2013 to March 2014 to investigate the Enterobacteriaceae from patients suffering from community-acquired urinary tract infections and then do sensitivity test for each of isolates. In this study out of 100 specimens 52 Enterobacteriaceae species were isolated from different clinics and hospitals in Port Sudan city. The specimens werecultured on CLED media ( cystinelactose electrolyte deficiency). identification was done by gram's stain and convential biochemical reactions. Then the anti–microbial sensitivity tests were done as the follows: (Ampicillin-Sulbactam "AS", Co. trimoxazole "BA", Ceftizoxime "CI", Chloramphenicol "CH", Cephalaxin "PR", Tetracycline "TE", Ciprofloxacin "CP", Amikacin "AK", Sparfloxacin "SC", Gatifloxacin "GF", Norflaxcin "NX) by Kirby-Bauer disc diffusion method. The study revealed that the most part of strains are sensitive to Chloramphenicol andAmikacin and resistant to Ampicillin –Sulpactam (AS). The identified Enterobacteriaceae were as follows; Escherichia coli 34 (65%), Klebsiella pneumonia 10 (19%), Klebsiella oxytoca 3(6%), Salmonella Para A 3 (6%), proteus mirabilis 1 (2%), Citrobacter 1 (2%).
VL  - 6
IS  - 1
ER  -

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Author Information

Abd Elrahman Mustafa Abd Elrahman Osman

Medical Laboratory Sciences Division, Port Sudan Ahlia College, Port Sudan, Sudan
Shingray Osman Hashim

Medical Laboratory Sciences Division, Port Sudan Ahlia College, Port Sudan, Sudan
Mohammed Abdall Musa

Medical Laboratory Sciences Division, Port Sudan Ahlia College, Port Sudan, Sudan
Omer Mohammed Tahir

Medical Laboratory Sciences Division, Port Sudan Ahlia College, Port Sudan, Sudan

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APA Style

Abd Elrahman Mustafa Abd Elrahman Osman, Shingray Osman Hashim, Mohammed Abdall Musa, Omer Mohammed Tahir. (2018). Isolation and Identification of Enterobacteriaceae from Patients with Community Acquired Urinary Tract Infection. American Journal of Health Research, 6(1), 25-31. https://doi.org/10.11648/j.ajhr.20180601.14

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ACS Style

Abd Elrahman Mustafa Abd Elrahman Osman; Shingray Osman Hashim; Mohammed Abdall Musa; Omer Mohammed Tahir. Isolation and Identification of Enterobacteriaceae from Patients with Community Acquired Urinary Tract Infection. Am. J. Health Res. 2018, 6(1), 25-31. doi: 10.11648/j.ajhr.20180601.14

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AMA Style

Abd Elrahman Mustafa Abd Elrahman Osman, Shingray Osman Hashim, Mohammed Abdall Musa, Omer Mohammed Tahir. Isolation and Identification of Enterobacteriaceae from Patients with Community Acquired Urinary Tract Infection. Am J Health Res. 2018;6(1):25-31. doi: 10.11648/j.ajhr.20180601.14

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@article{10.11648/j.ajhr.20180601.14,
  author = {Abd Elrahman Mustafa Abd Elrahman Osman and Shingray Osman Hashim and Mohammed Abdall Musa and Omer Mohammed Tahir},
  title = {Isolation and Identification of Enterobacteriaceae from Patients with Community Acquired Urinary Tract Infection},
  journal = {American Journal of Health Research},
  volume = {6},
  number = {1},
  pages = {25-31},
  doi = {10.11648/j.ajhr.20180601.14},
  url = {https://doi.org/10.11648/j.ajhr.20180601.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20180601.14},
  abstract = {The study was carried out in red sea state during the period from November 2013 to March 2014 to investigate the Enterobacteriaceae from patients suffering from community-acquired urinary tract infections and then do sensitivity test for each of isolates. In this study out of 100 specimens 52 Enterobacteriaceae species were isolated from different clinics and hospitals in Port Sudan city. The specimens werecultured on CLED media ( cystinelactose electrolyte deficiency). identification was done by gram's stain and convential biochemical reactions. Then the anti–microbial sensitivity tests were done as the follows: (Ampicillin-Sulbactam "AS", Co. trimoxazole "BA", Ceftizoxime "CI", Chloramphenicol "CH", Cephalaxin "PR", Tetracycline "TE", Ciprofloxacin "CP", Amikacin "AK", Sparfloxacin "SC", Gatifloxacin "GF", Norflaxcin "NX) by Kirby-Bauer disc diffusion method. The study revealed that the most part of strains are sensitive to Chloramphenicol andAmikacin and resistant to Ampicillin –Sulpactam (AS). The identified Enterobacteriaceae were as follows; Escherichia coli 34 (65%), Klebsiella pneumonia 10 (19%), Klebsiella oxytoca 3(6%), Salmonella Para A 3 (6%), proteus mirabilis 1 (2%), Citrobacter 1 (2%).},
 year = {2018}
}

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TY  - JOUR
T1  - Isolation and Identification of Enterobacteriaceae from Patients with Community Acquired Urinary Tract Infection
AU  - Abd Elrahman Mustafa Abd Elrahman Osman
AU  - Shingray Osman Hashim
AU  - Mohammed Abdall Musa
AU  - Omer Mohammed Tahir
Y1  - 2018/01/22
PY  - 2018
N1  - https://doi.org/10.11648/j.ajhr.20180601.14
DO  - 10.11648/j.ajhr.20180601.14
T2  - American Journal of Health Research
JF  - American Journal of Health Research
JO  - American Journal of Health Research
SP  - 25
EP  - 31
PB  - Science Publishing Group
SN  - 2330-8796
UR  - https://doi.org/10.11648/j.ajhr.20180601.14
AB  - The study was carried out in red sea state during the period from November 2013 to March 2014 to investigate the Enterobacteriaceae from patients suffering from community-acquired urinary tract infections and then do sensitivity test for each of isolates. In this study out of 100 specimens 52 Enterobacteriaceae species were isolated from different clinics and hospitals in Port Sudan city. The specimens werecultured on CLED media ( cystinelactose electrolyte deficiency). identification was done by gram's stain and convential biochemical reactions. Then the anti–microbial sensitivity tests were done as the follows: (Ampicillin-Sulbactam "AS", Co. trimoxazole "BA", Ceftizoxime "CI", Chloramphenicol "CH", Cephalaxin "PR", Tetracycline "TE", Ciprofloxacin "CP", Amikacin "AK", Sparfloxacin "SC", Gatifloxacin "GF", Norflaxcin "NX) by Kirby-Bauer disc diffusion method. The study revealed that the most part of strains are sensitive to Chloramphenicol andAmikacin and resistant to Ampicillin –Sulpactam (AS). The identified Enterobacteriaceae were as follows; Escherichia coli 34 (65%), Klebsiella pneumonia 10 (19%), Klebsiella oxytoca 3(6%), Salmonella Para A 3 (6%), proteus mirabilis 1 (2%), Citrobacter 1 (2%).
VL  - 6
IS  - 1
ER  -

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