| Peer-Reviewed

Immunomodulatory Activity of Biofield Energy Healing Based Herbomineral Formulation in Sprague Dawley Rats: Evidence for Humoral and Cell-Mediated Responses

Received: 30 October 2017    Accepted: 11 November 2017    Published: 11 December 2017
Views:       Downloads:
Abstract

Herbomineral formulations are used extensively in the world-wide for the prevention and treatment of a wide range of disorders. A new proprietary herbomineral formulation was formulated consisted of an ashwagandha root extract and minerals (zinc, magnesium, and selenium). The present study was aimed to evaluate the impact of the Biofield Energy Treated herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The experimental parameters studies were humoral immune response (primary and secondary titre), delayed type hypersensitivity reaction, animal weight parameters, feed and water intake, histopathology, hematological and serum biochemistry. The humoral immune response exhibited that both the primary and secondary hemagglutination (HA) antibody titre levels were significantly altered by 14.79% and 12.89%, respectively in the Biofield Energy Treated test formulation group (G4) compared to the disease control group (G2). The delayed type hypersensitivity (DTH) response was significantly (p≤0.05) increased by 55.56% in the G4 group compared to the G2 group. The hematological results revealed that the platelet count was significantly increased by 8.46% in the G4 group with respect to the G2 group. Moreover, the level of phosphorus was significantly increased by 11.16% and 16.35% in the levamisole (G3) and G4 groups, respectively compared to the G2 group. In conclusion, the Biofield Energy Treated Test Formulation would be the powerful immunomodulatory product, which was found safe at the tested doses. Therefore, The Trivedi Effect®-Biofield Energy Healing based herbomineral formulation can be applied to potentiate the immune system that helps to fight against many infectious diseases. Overall, these data suggest that the Biofield Energy Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

Published in American Journal of Health Research (Volume 5, Issue 6)
DOI 10.11648/j.ajhr.20170506.14
Page(s) 183-192
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Biofield Energy Healing, Immunomodulation, Herbomineral Formulation, Humoral Immune Response, Antibody Titre, Delay Type Hypersensitivity, Stress Management and Anti-aging

References
[1] Phillipson JD (1994) Natural products as drugs. Trans Royal Soc Trop Med Hyg 88: S17-S19.
[2] Khan S, Balick MJ (2001) Therapeutic plants of Ayurveda: A review of selected clinical and other Studies for 166 Species. J Altern Complement Med 7: 405-515.
[3] Patwardhan B, Chorghade M (2004) Ayurveda and natural products drug discovery. Curr Sci 86: 789-798.
[4] Patel P, Basheeruddin Asdaq SM (2010) Immunomodulatory activity of methanolic fruit extract of Aegle marmelos in experimental animals. Saudi Pharm J 18: 161-165.
[5] Heroor S, Beknal A, Mahurkar N (2012) Preliminary investigation for immunomodulation of methanolic extracts of leaves and flowers of Pongamia glabra Vent. in mice model. Adv Lif Sci 2: 170-173.
[6] Farhath S, Vijaya PP, Vima M (2013) Immunomodulatory activity of geranial, geranial acetate, gingerol, and eugenol essential oils: Evidence for humoral and cell-mediated responses. Avicenna J Phytomed 3: 224-230.
[7] MacLennan AH, Wilson DH, Taylor AW (1996) Prevalence and cost of alternative medicine in Australia. Lancet 347: 569 - 573.
[8] Houston M (2014) The role of nutrition and nutraceutical supplements in the treatment of hypertension. World J Cardiol 6: 38-66.
[9] Bishop WM, Zubeck HM (2012) Evaluation of microalgae for use as nutraceuticals and nutritional supplements. J Nutr Food Sci 2: 147.
[10] Houston M (2013) Nutrition and nutraceutical supplements for the treatment of hypertension: Part I. J Clin Hypertens (Greenwich) 15: 752-757.
[11] Qureshi NA, Al-Bedah AM (2013) Mood disorders and complementary and alternative medicine: A literature review. Neuropsychiatr Dis Treat 9: 639-658.
[12] Lutgendorf SK, Mullen-Houser E, Russell D, Degeest K, Jacobson G, Hart L, Bender D, Anderson B, Buekers TE, Goodheart MJ, Antoni MH, Sood AK, Lubaroff DM (2010) Preservation of immune function in cervical cancer patients during chemoradiation using a novel integrative approach. Brain, behavior, and immunity 24: 1231-1240.
[13] Ironson G, Field T, Scafidi F, Hashimoto M, Kumar M, Kumar A, Price A, Goncalves A, Burman I, Tetenman C, Patarca R, Fletcher MA (1996) Massage therapy is associated with enhancement of the immune system's cytotoxic capacity. Int J Neurosci 84: 205-217.
[14] Koithan M (2009) Introducing complementary and alternative therapies. J Nurse Pract 5: 18-20.
[15] Jain S, Hammerschlag R, Mills P, Cohen L, Krieger R, Vieten C, Lutgendorf S (2015) Clinical studies of biofield therapies: Summary, methodological challenges, and recommendations. Glob Adv Health Med 4: 58-66.
[16] Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med 8: 703-717.
[17] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4: 141.
[18] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In vitro evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther 7: 253-257.
[19] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) In vitro evaluation of biofield treatment on Enterobacter cloacae: Impact on antimicrobial susceptibility and biotype. J Bacteriol Parasitol 6: 241.
[20] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) Evaluation of biofield modality on viral load of hepatitis B and C Viruses. J Antivir Antiretrovir 7: 083-088.
[21] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An impact of biofield treatment: Antimycobacterial susceptibility potential using BACTEC 460/MGIT-TB System. Mycobact Dis 5: 189.
[22] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Antimicrobial sensitivity, biochemical characteristics and biotyping of Staphylococcus saprophyticus: An impact of biofield energy treatment. J Women’s Health Care 4: 271.
[23] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of antibiogram, genotype and phylogenetic analysis of biofield treated Nocardia otitidis. Biol Syst Open Access 4: 143.
[24] Trivedi MK, Branton A, Trivedi D, Nayak G, Charan S, Jana S (2015) Phenotyping and 16S rDNA analysis after biofield treatment on Citrobacter braakii: A urinary pathogen. J Clin Med Genom 3: 129.
[25] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Spectroscopic characterization of chloramphenicol and tetracycline: An impact of biofield. Pharm Anal Acta 6:395.
[26] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Spectroscopic characterization of biofield treated metronidazole and tinidazole. Med Chem 5: 340-344.
[27] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Effect of biofield treatment on spectral properties of paracetamol and piroxicam. Chem Sci J 6: 98.
[28] Trivedi MK, Branton A, Trivedi D, Shettigar H, Bairwa K, Jana S (2015) Fourier transform infrared and ultraviolet-visible spectroscopic characterization of biofield treated salicylic acid and sparfloxacin. Nat Prod Chem Res 3: 186.
[29] Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2016) Molecular analysis of biofield treated eggplant and watermelon crops. Adv Crop Sci Tech 4: 208.
[30] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Morphological characterization, quality, yield and DNA fingerprinting of biofield energy treated alphonso mango (Mangifera indica L.). Journal of Food and Nutrition Sciences 3: 245-250.
[31] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of plant growth, yield and yield attributes of biofield energy treated mustard (Brassica juncea) and chick pea (Cicer arietinum) seeds. Agriculture, Forestry and Fisheries 4: 291-295.
[32] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of plant growth regulator, immunity and DNA fingerprinting of biofield energy treated mustard seeds (Brassica juncea). Agriculture, Forestry and Fisheries 4: 269-274.
[33] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Jana S (2015) Characterization of physical and structural properties of aluminum carbide powder: Impact of biofield treatment. J Aeronaut Aerospace Eng 4: 142.
[34] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) Impact of biofield treatment on atomic and structural characteristics of barium titanate powder. Ind Eng Manage 4: 166.
[35] Trivedi MK, Patil S, Nayak G, Jana S, Latiyal O (2015) Influence of biofield treatment on physical, structural and spectral properties of boron nitride. J Material Sci Eng 4: 181.
[36] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) Characterization of physical and structural properties of brass powder after biofield treatment. J Powder Metall Min 4: 134.
[37] Ladics GS (2007) Primary immune response to sheep red blood cells (SRBC) as the conventional T-cell dependent antibody response (TDAR) test. J Immunotoxicol 4: 149-152.
[38] Joharapurkar AA, Zambad SP, Wanjari MM, Umathe SN (2003) In vivo evaluation of antioxidant activity of alcoholic extract of Rubia cordifolia Linn. and its influence on ethanol-induced immunosuppression. Indian J Pharmacol 35: 232-236.
[39] Nelson DS, Mildenhall P (1967) Studies on cytophilic antibodies. 1. The production by mice of macrophage cytophilic antibodies to sheep erythrocytes: relationship to the production of other antibodies and the development of delayed-type hypersensitivity. Aust J Exp Biol Med Sci 45: 113-130.
[40] Saiki I, Tanio Y, Yamawaki M, Uemiya M, Kobayashi S, Fukuda T, Yukimasa H, Yamamura Y, Azuma I (1981) Adjuvant activities of quinonyl-N-acetyl muramyl dipeptides in mice and guinea pigs. Infect Immun 31: 114-121.
[41] Feldman BF, Zinkl JG, Jain VC. Laboratory techniques for avian hematology,” in Schalm’s Veterinary Hematology, (5th Edn) Lippincott Williams & Wilkins, Toronto, Canada, 2000.
[42] Chanda S, Dave R, Kaneria M, Shukla V (2012) Acute oral toxicity of Polyalthia longifolia var. pendula leaf extract in wistar albino rats. Pharm Biol 50: 1408-1415.
[43] OECD, OECD Guideline for Testing of Chemicals, vol. 420, Organization for Economic Cooperation and Development, Paris, France, 1992.
[44] Sellers RS, Morton D, Michael B, Roome N, Johnson JK, Yano B, Perry R, Schafer K (2007) Society of toxicologic pathology position paper: Organ weight recommendations for toxicology studies. Toxicol Pathol 35: 751-755.
[45] Bailey SA, Zidell RH, Perry RW (2004) Relationships between organ weight and body/brain weight in the rat: What is the best analytical endpoint? Toxicol Pathol 32: 448-466.
[46] Naik SR, Gavankar C, Thakare VN (2015) Immunomodulatory activity of Withania somnifera and Curcuma longa in animal models: Modulation of cytokines functioning. Pharmacologia 6: 168-177.
[47] Nagalakshmi D, Sridhar K, Parashuramulu S (2015) Replacement of inorganic zinc with lower levels of organic zinc (zinc nicotinate) on performance, hematological and serum biochemical constituents, antioxidants status and immune responses in rats. Vet World 8: 1156-1162.
[48] Agarwal R, Diwanay S, Patki P, Patwardhan B (1999) Studies on immunomodulatory activity of Withania somnifera (ashwagandha) extracts in experimental immune inflammation. J Ethnopharmacol 67: 27-35.
[49] Ziauddin M, Phansalkar N, Patki P, Diwanay S, Patwardhan B (1996) Studies on immunomodulatory effects of a ashwagandha. J Ethnopharmacol 50: 69-76.
[50] Amresh GR, Singh PN, Rao CV (2008) Toxicological screening of traditional medicine Laghupatha (Cissampelos pareira) in experimental animals. J Ethnopharmacol 116: 454-460.
[51] Clarke TC, Black LI, Stussman BJ, Barnes PM, Nahin RL (2015) Trends in the use of complementary health approaches among adults: Unites States, 2002-2012. National health statistics reports. no 79. Hyattsville, MD: National Center for Health Statistics.
Cite This Article
  • APA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Michael Peter Ellis, et al. (2017). Immunomodulatory Activity of Biofield Energy Healing Based Herbomineral Formulation in Sprague Dawley Rats: Evidence for Humoral and Cell-Mediated Responses. American Journal of Health Research, 5(6), 183-192. https://doi.org/10.11648/j.ajhr.20170506.14

    Copy | Download

    ACS Style

    Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Michael Peter Ellis, et al. Immunomodulatory Activity of Biofield Energy Healing Based Herbomineral Formulation in Sprague Dawley Rats: Evidence for Humoral and Cell-Mediated Responses. Am. J. Health Res. 2017, 5(6), 183-192. doi: 10.11648/j.ajhr.20170506.14

    Copy | Download

    AMA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Michael Peter Ellis, et al. Immunomodulatory Activity of Biofield Energy Healing Based Herbomineral Formulation in Sprague Dawley Rats: Evidence for Humoral and Cell-Mediated Responses. Am J Health Res. 2017;5(6):183-192. doi: 10.11648/j.ajhr.20170506.14

    Copy | Download

  • @article{10.11648/j.ajhr.20170506.14,
      author = {Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Michael Peter Ellis and James Jeffery Peoples and James Joseph Meuer and Johanne Dodon and John Lawrence Griffin and John Suzuki and Joseph Michael Foty and Judy Weber and Julia Grace McCammon and Karen Brynes Allen and Kathryn Regina Sweas and Lezley Jo-Anne Wright and Lisa A. Knoll and Madeline E. Michaels and Madeline E. Michaels and Mark E. Stutheit and Michelle Barnard and Muriel Mae Ranger and Paromvong Sinbandhit and V. J. Kris Elig and Sambhu Charan Mondal and Snehasis Jana},
      title = {Immunomodulatory Activity of Biofield Energy Healing Based Herbomineral Formulation in Sprague Dawley Rats: Evidence for Humoral and Cell-Mediated Responses},
      journal = {American Journal of Health Research},
      volume = {5},
      number = {6},
      pages = {183-192},
      doi = {10.11648/j.ajhr.20170506.14},
      url = {https://doi.org/10.11648/j.ajhr.20170506.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20170506.14},
      abstract = {Herbomineral formulations are used extensively in the world-wide for the prevention and treatment of a wide range of disorders. A new proprietary herbomineral formulation was formulated consisted of an ashwagandha root extract and minerals (zinc, magnesium, and selenium). The present study was aimed to evaluate the impact of the Biofield Energy Treated herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The experimental parameters studies were humoral immune response (primary and secondary titre), delayed type hypersensitivity reaction, animal weight parameters, feed and water intake, histopathology, hematological and serum biochemistry. The humoral immune response exhibited that both the primary and secondary hemagglutination (HA) antibody titre levels were significantly altered by 14.79% and 12.89%, respectively in the Biofield Energy Treated test formulation group (G4) compared to the disease control group (G2). The delayed type hypersensitivity (DTH) response was significantly (p≤0.05) increased by 55.56% in the G4 group compared to the G2 group. The hematological results revealed that the platelet count was significantly increased by 8.46% in the G4 group with respect to the G2 group. Moreover, the level of phosphorus was significantly increased by 11.16% and 16.35% in the levamisole (G3) and G4 groups, respectively compared to the G2 group. In conclusion, the Biofield Energy Treated Test Formulation would be the powerful immunomodulatory product, which was found safe at the tested doses. Therefore, The Trivedi Effect®-Biofield Energy Healing based herbomineral formulation can be applied to potentiate the immune system that helps to fight against many infectious diseases. Overall, these data suggest that the Biofield Energy Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.},
     year = {2017}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Immunomodulatory Activity of Biofield Energy Healing Based Herbomineral Formulation in Sprague Dawley Rats: Evidence for Humoral and Cell-Mediated Responses
    AU  - Mahendra Kumar Trivedi
    AU  - Alice Branton
    AU  - Dahryn Trivedi
    AU  - Gopal Nayak
    AU  - Michael Peter Ellis
    AU  - James Jeffery Peoples
    AU  - James Joseph Meuer
    AU  - Johanne Dodon
    AU  - John Lawrence Griffin
    AU  - John Suzuki
    AU  - Joseph Michael Foty
    AU  - Judy Weber
    AU  - Julia Grace McCammon
    AU  - Karen Brynes Allen
    AU  - Kathryn Regina Sweas
    AU  - Lezley Jo-Anne Wright
    AU  - Lisa A. Knoll
    AU  - Madeline E. Michaels
    AU  - Madeline E. Michaels
    AU  - Mark E. Stutheit
    AU  - Michelle Barnard
    AU  - Muriel Mae Ranger
    AU  - Paromvong Sinbandhit
    AU  - V. J. Kris Elig
    AU  - Sambhu Charan Mondal
    AU  - Snehasis Jana
    Y1  - 2017/12/11
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajhr.20170506.14
    DO  - 10.11648/j.ajhr.20170506.14
    T2  - American Journal of Health Research
    JF  - American Journal of Health Research
    JO  - American Journal of Health Research
    SP  - 183
    EP  - 192
    PB  - Science Publishing Group
    SN  - 2330-8796
    UR  - https://doi.org/10.11648/j.ajhr.20170506.14
    AB  - Herbomineral formulations are used extensively in the world-wide for the prevention and treatment of a wide range of disorders. A new proprietary herbomineral formulation was formulated consisted of an ashwagandha root extract and minerals (zinc, magnesium, and selenium). The present study was aimed to evaluate the impact of the Biofield Energy Treated herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The experimental parameters studies were humoral immune response (primary and secondary titre), delayed type hypersensitivity reaction, animal weight parameters, feed and water intake, histopathology, hematological and serum biochemistry. The humoral immune response exhibited that both the primary and secondary hemagglutination (HA) antibody titre levels were significantly altered by 14.79% and 12.89%, respectively in the Biofield Energy Treated test formulation group (G4) compared to the disease control group (G2). The delayed type hypersensitivity (DTH) response was significantly (p≤0.05) increased by 55.56% in the G4 group compared to the G2 group. The hematological results revealed that the platelet count was significantly increased by 8.46% in the G4 group with respect to the G2 group. Moreover, the level of phosphorus was significantly increased by 11.16% and 16.35% in the levamisole (G3) and G4 groups, respectively compared to the G2 group. In conclusion, the Biofield Energy Treated Test Formulation would be the powerful immunomodulatory product, which was found safe at the tested doses. Therefore, The Trivedi Effect®-Biofield Energy Healing based herbomineral formulation can be applied to potentiate the immune system that helps to fight against many infectious diseases. Overall, these data suggest that the Biofield Energy Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.
    VL  - 5
    IS  - 6
    ER  - 

    Copy | Download

Author Information
  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Sections