Asia-Pacific Journal of Chemistry

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Preparation and Application of Molecular Imprinting Solid-Phase Microextraction Coating Based on Cytokinins

Received: 28 May 2019    Accepted: 13 June 2019    Published: 09 July 2019
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Abstract

As a class of common plant hormones, cytokinins (CTKs) can greatly promote cell division and have wide application in agricultural production. Therefore, it is necessary to monitor the residue of CTKs in food or environment. Molecular Imprinting polymer (MIP) with kinetin-6-furfurylaminopurine (6-KT) as the template molecule was prepared using multiple polymerizations. The MIP was used as the fiber coating of solid-phase microextraction (SPME), and then molecular imprinting solid-phase microextraction (MISPME) assay was developed. A series of MISPME extraction conditions were optimized in detail. The extraction was performed at 60°C for 30 min with n-hexane as the extraction solvent and stirring speed of 400 rpm, with 15 µL ethanol as the desorption solvent and desorption for 10 min. For vegetable and environmental water samples, two kinds of CTKs were analyzed by high performance liquid chromatography with UV detector. The enrichment factors of kinetin-6-furfurylaminopurine (6-KT) and N6-benzyladenine (6-BA) were 154 and 163, with whose detection limits of 0.3 ng/mL and 0.5 ng/mL, respectively. The proposed method was sensitive and reproducible, and it was suitable to the pretreatment and selective enrichment of plant hormones.

Published in Asia-Pacific Journal of Chemistry (Volume 1, Issue 1, March 2019)
Page(s) 13-18
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Molecular Imprinting Technique, Solid-Phase Microextraction, Cytokinin, High Performance Liquid Chromatography

References
[1] Koprna Radoslav, De Diego Nuria, Dundálková Lucie, Spíchal Lukáš. Use of cytokinins as agrochemicals [J]. Bioorganic & Medicinal Chemistry. 2016, 24(3): 484-492。
[2] 樊彪,赵江哲.细胞分裂素研究进展及其在作物生产中的应 [J].浙江农业科学,2017,58(8):1411-1414。
[3] 吴艳歌,毕波,柴明良.细胞分裂素对沟叶结缕草愈伤组织生长及其抗氧化系统的影响[J].核农学报,2016,30(7):1288-1295。
[4] 中华人民共和国国家卫生部,农业部.食品安全国家标准食品中农药最大残留限量:GB 2763-2012[S].北京:中国标准出版社,2012。
[5] Bel Bruno Joseph J. Molecularly imprinted polymers [J]. Chemical reviews, 2019, 119: 94-119.
[6] 柯珍,朱华,钟世安,邹登峰.分子印迹技术及其应用研究进展[J].化学研究与应用,2018,30(6):865-874。
[7] Guizhen Li, Kyung Ho Row. Recent applications of molecularly imprinted polymers (MIPs) on micro-extraction techniques [J]. Separation and Purification Reviews, 2018, 47(1): 1-18.
[8] Shuyao Huang, Jianqiao Xu, Jiating Zheng, Fang Zhu, Lijun Xie, Gangfeng Ouyang. Synthesis and application of magnetic molecularly imprinted polymers in sample preparation [J]. Analytical and Bioanalytical Chemistry, 2018, 410(17): 3991-4014.
[9] Koster E H M, Crescenzi C, Den H W, et al. Fibers coated with molecularly imprinted polymers for solid-phase microextraction [J]. Analytical Chemistry, 2001, 73(13): 3140-3145.
[10] Fuyou Du, Guihua Ruan, Shenghua Liang, Fuwei Xie, Huwei Liu. Monolithic molecularly imprinted solid-phase extraction for the selective determination of trace cytokinins in plant samples with liquid chromatography-electrospray tandem mass spectrometry [J]. Analytical and Bioanalytical Chemistry, 2012, 404: 489-501.
[11] 孙林,杜甫佑,阮贵华,黄绎嘉.分子印迹整体柱富集-高效液相色谱法测定植物中的痕量细胞分裂素[J].色谱,2013,31(4): 392-396。
[12] 张兰,卢巧梅,范良彪,陈国南.一种6-糠氨基腺嘌呤分子印迹聚合物及其应用[P].中国:ZL201210190656.5,2013-12-11。
[13] Xiangli Sun, Jia He, Guorui Cai, Anqing Lin, Wenjie Zheng, Xuan Liu, Langxing Chen, Xiwen He, Yukui Zhang. Room temperature ionic liquid-mediated molecularly imprinted polymer monolith for the selective recognition of quinolones in pork samples [J]. Journal of Separation Science, 2010, 33: 3786-3793.
Author Information
  • Testing Center of Fuzhou University, Fuzhou, China; Ministry of Education Key Laboratory of Analytical Science and Food Safety and Biology, Fuzhou University, Fuzhou, China

  • Chemical and Materials College, Quanzhou Normal University, Quanzhou, China

  • Ministry of Education Key Laboratory of Analytical Science and Food Safety and Biology, Fuzhou University, Fuzhou, China

Cite This Article
  • APA Style

    Lu Qiaomei, Fan Liangbiao, Zhang Lan. (2019). Preparation and Application of Molecular Imprinting Solid-Phase Microextraction Coating Based on Cytokinins. Asia-Pacific Journal of Chemistry, 1(1), 13-18.

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    ACS Style

    Lu Qiaomei; Fan Liangbiao; Zhang Lan. Preparation and Application of Molecular Imprinting Solid-Phase Microextraction Coating Based on Cytokinins. Asia-Pac. J. Chem. 2019, 1(1), 13-18.

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    AMA Style

    Lu Qiaomei, Fan Liangbiao, Zhang Lan. Preparation and Application of Molecular Imprinting Solid-Phase Microextraction Coating Based on Cytokinins. Asia-Pac J Chem. 2019;1(1):13-18.

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  • @article{10040015,
      author = {Lu Qiaomei and Fan Liangbiao and Zhang Lan},
      title = {Preparation and Application of Molecular Imprinting Solid-Phase Microextraction Coating Based on Cytokinins},
      journal = {Asia-Pacific Journal of Chemistry},
      volume = {1},
      number = {1},
      pages = {13-18},
      url = {http://www.sciencepg.com/article/10040015},
      abstract = {As a class of common plant hormones, cytokinins (CTKs) can greatly promote cell division and have wide application in agricultural production. Therefore, it is necessary to monitor the residue of CTKs in food or environment. Molecular Imprinting polymer (MIP) with kinetin-6-furfurylaminopurine (6-KT) as the template molecule was prepared using multiple polymerizations. The MIP was used as the fiber coating of solid-phase microextraction (SPME), and then molecular imprinting solid-phase microextraction (MISPME) assay was developed. A series of MISPME extraction conditions were optimized in detail. The extraction was performed at 60°C for 30 min with n-hexane as the extraction solvent and stirring speed of 400 rpm, with 15 µL ethanol as the desorption solvent and desorption for 10 min. For vegetable and environmental water samples, two kinds of CTKs were analyzed by high performance liquid chromatography with UV detector. The enrichment factors of kinetin-6-furfurylaminopurine (6-KT) and N6-benzyladenine (6-BA) were 154 and 163, with whose detection limits of 0.3 ng/mL and 0.5 ng/mL, respectively. The proposed method was sensitive and reproducible, and it was suitable to the pretreatment and selective enrichment of plant hormones.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Preparation and Application of Molecular Imprinting Solid-Phase Microextraction Coating Based on Cytokinins
    AU  - Lu Qiaomei
    AU  - Fan Liangbiao
    AU  - Zhang Lan
    Y1  - 2019/07/09
    PY  - 2019
    T2  - Asia-Pacific Journal of Chemistry
    JF  - Asia-Pacific Journal of Chemistry
    JO  - Asia-Pacific Journal of Chemistry
    SP  - 13
    EP  - 18
    PB  - Science Publishing Group
    UR  - http://www.sciencepg.com/article/10040015
    AB  - As a class of common plant hormones, cytokinins (CTKs) can greatly promote cell division and have wide application in agricultural production. Therefore, it is necessary to monitor the residue of CTKs in food or environment. Molecular Imprinting polymer (MIP) with kinetin-6-furfurylaminopurine (6-KT) as the template molecule was prepared using multiple polymerizations. The MIP was used as the fiber coating of solid-phase microextraction (SPME), and then molecular imprinting solid-phase microextraction (MISPME) assay was developed. A series of MISPME extraction conditions were optimized in detail. The extraction was performed at 60°C for 30 min with n-hexane as the extraction solvent and stirring speed of 400 rpm, with 15 µL ethanol as the desorption solvent and desorption for 10 min. For vegetable and environmental water samples, two kinds of CTKs were analyzed by high performance liquid chromatography with UV detector. The enrichment factors of kinetin-6-furfurylaminopurine (6-KT) and N6-benzyladenine (6-BA) were 154 and 163, with whose detection limits of 0.3 ng/mL and 0.5 ng/mL, respectively. The proposed method was sensitive and reproducible, and it was suitable to the pretreatment and selective enrichment of plant hormones.
    VL  - 1
    IS  - 1
    ER  - 

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