Biochemical experimental teaching is an indispensable key link in the process of professional training in the college of life sciences. To develop comprehensive and designing experiments courses can improve the quality of biochemical experiment teaching. Fusion protein expression and purification technology is an important research means to research protein structure and function. In the field of protein science, getting high purity and bioactive proteins often is the basis of protein structure and function studies. Affinity chromatography is one of effective methods of protein separation and purification. Which fusion protein purification technology acts as the core, our teaching platform has opened an innovative, comprehensive experimental teaching course called "Purification of His-tagged proteins using Ni2+ Sepharose affinity chromatography ". This paper expounds the implementation of the teaching goal, the key and difficult, experimental principle, experimental procedures and results, etc. Practice shows that the experimental teaching course can subtly make an organic integration of multi-disciplinary knowledge points, just like biochemistry, molecular biology, gene engineering technology and so on. Using this strategy, we can not only consolidate and strengthen the basic knowledge of biochemistry theory students have studied, but also can improve their comprehensive abilities to experimental designs and cultivate their innovative spirits meanwhile. The innovative experiment course, with moderate difficulty, can be widely applied in the other colleges those have biochemistry major.
| Published in | Asia-Pacific Journal of Biology (Volume 2, Issue 2) |
| Page(s) | 15-21 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Biochemistry, Experiment Teaching, Fusion Proteins, Educational Reform
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APA Style
Qian Shuyi, Kang Yujia. (2020). Application of Fusion Protein Expression and Purification Technology in Innovative Experimental Teaching of Biochemistry. Asia-Pacific Journal of Biology, 2(2), 15-21.
ACS Style
Qian Shuyi; Kang Yujia. Application of Fusion Protein Expression and Purification Technology in Innovative Experimental Teaching of Biochemistry. Asia-Pac. J. Biol. 2020, 2(2), 15-21.
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Download@article{10045047,
author = {Qian Shuyi and Kang Yujia},
title = {Application of Fusion Protein Expression and Purification Technology in Innovative Experimental Teaching of Biochemistry},
journal = {Asia-Pacific Journal of Biology},
volume = {2},
number = {2},
pages = {15-21},
url = {https://www.sciencepublishinggroup.com/article/10045047},
abstract = {Biochemical experimental teaching is an indispensable key link in the process of professional training in the college of life sciences. To develop comprehensive and designing experiments courses can improve the quality of biochemical experiment teaching. Fusion protein expression and purification technology is an important research means to research protein structure and function. In the field of protein science, getting high purity and bioactive proteins often is the basis of protein structure and function studies. Affinity chromatography is one of effective methods of protein separation and purification. Which fusion protein purification technology acts as the core, our teaching platform has opened an innovative, comprehensive experimental teaching course called "Purification of His-tagged proteins using Ni2+ Sepharose affinity chromatography ". This paper expounds the implementation of the teaching goal, the key and difficult, experimental principle, experimental procedures and results, etc. Practice shows that the experimental teaching course can subtly make an organic integration of multi-disciplinary knowledge points, just like biochemistry, molecular biology, gene engineering technology and so on. Using this strategy, we can not only consolidate and strengthen the basic knowledge of biochemistry theory students have studied, but also can improve their comprehensive abilities to experimental designs and cultivate their innovative spirits meanwhile. The innovative experiment course, with moderate difficulty, can be widely applied in the other colleges those have biochemistry major.},
year = {2020}
}
TY - JOUR T1 - Application of Fusion Protein Expression and Purification Technology in Innovative Experimental Teaching of Biochemistry AU - Qian Shuyi AU - Kang Yujia Y1 - 2020/03/02 PY - 2020 T2 - Asia-Pacific Journal of Biology JF - Asia-Pacific Journal of Biology JO - Asia-Pacific Journal of Biology SP - 15 EP - 21 PB - Science Publishing Group UR - http://www.sciencepg.com/article/10045047 AB - Biochemical experimental teaching is an indispensable key link in the process of professional training in the college of life sciences. To develop comprehensive and designing experiments courses can improve the quality of biochemical experiment teaching. Fusion protein expression and purification technology is an important research means to research protein structure and function. In the field of protein science, getting high purity and bioactive proteins often is the basis of protein structure and function studies. Affinity chromatography is one of effective methods of protein separation and purification. Which fusion protein purification technology acts as the core, our teaching platform has opened an innovative, comprehensive experimental teaching course called "Purification of His-tagged proteins using Ni2+ Sepharose affinity chromatography ". This paper expounds the implementation of the teaching goal, the key and difficult, experimental principle, experimental procedures and results, etc. Practice shows that the experimental teaching course can subtly make an organic integration of multi-disciplinary knowledge points, just like biochemistry, molecular biology, gene engineering technology and so on. Using this strategy, we can not only consolidate and strengthen the basic knowledge of biochemistry theory students have studied, but also can improve their comprehensive abilities to experimental designs and cultivate their innovative spirits meanwhile. The innovative experiment course, with moderate difficulty, can be widely applied in the other colleges those have biochemistry major. VL - 2 IS - 2 ER -
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