Effects of Concentrations of IBA, NAA and 6-BA on the Proliferation and Rooting Culture of Chamomile

Bai Yanrong; Wang Jinying

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Effects of Concentrations of IBA, NAA and 6-BA on the Proliferation and Rooting Culture of Chamomile

Bai Yanrong, Wang Jinying

Published in Asia-Pacific Journal of Biology (Volume 3, Issue 1)

Received: 7 January 2020 Accepted: 9 June 2020 Published: 28 July 2020

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Abstract

Objective: To select the best culture medium for proliferation and root culture. Method: Using aseptic buds obtained from the first generation of culture as the experimental material, different concentrations of auxin (IBA, NAA) and cytokinin 6-BA were combined to conduct proliferation culture and rooting culture of Chamomile. Result: The results showed that the aseptic buds of primary culture were transferred to MS +1.3 mg/L6-BA +0.2 mg LIBA +6.2 g/L agarose +30 g/L sucrose + 0.1 g/L activated carbon culture medium after initial screening. After 40 days of culture, the proliferation effect is the best, the proliferation coefficient is 6.2, the average plant height is 3.4 cm, and the plant grows strongly; The aseptic buds obtained from the initial culture were transferred to the 1/2 MS + 0.4 mg/LIBA + 0.1 mg/L NAA +6.2 g/L agar + 30 g/L sucrose Rhizome medium, and after 30 days of culture, the root rate reached 96 , The number of roots per plant reached 8.2, the average root length was 3.6 cm, and the root growth was the best.

Published in	Asia-Pacific Journal of Biology (Volume 3, Issue 1)
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

African Chrysanthemum, Proliferative Culture, Rooting Culture, 6-BA Concentration

References

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APA Style

Bai Yanrong, Wang Jinying. (2020). Effects of Concentrations of IBA, NAA and 6-BA on the Proliferation and Rooting Culture of Chamomile. Asia-Pacific Journal of Biology, 3(1), 1-6.

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ACS Style

Bai Yanrong; Wang Jinying. Effects of Concentrations of IBA, NAA and 6-BA on the Proliferation and Rooting Culture of Chamomile. Asia-Pac. J. Biol. 2020, 3(1), 1-6.

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AMA Style

Bai Yanrong, Wang Jinying. Effects of Concentrations of IBA, NAA and 6-BA on the Proliferation and Rooting Culture of Chamomile. Asia-Pac J Biol. 2020;3(1):1-6.

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@article{10046032,
  author = {Bai Yanrong and Wang Jinying},
  title = {Effects of Concentrations of IBA, NAA and 6-BA on the Proliferation and Rooting Culture of Chamomile},
  journal = {Asia-Pacific Journal of Biology},
  volume = {3},
  number = {1},
  pages = {1-6},
  url = {https://www.sciencepublishinggroup.com/article/10046032},
  abstract = {Objective: To select the best culture medium for proliferation and root culture. Method: Using aseptic buds obtained from the first generation of culture as the experimental material, different concentrations of auxin (IBA, NAA) and cytokinin 6-BA were combined to conduct proliferation culture and rooting culture of Chamomile. Result: The results showed that the aseptic buds of primary culture were transferred to MS +1.3 mg/L6-BA +0.2 mg LIBA +6.2 g/L agarose +30 g/L sucrose + 0.1 g/L activated carbon culture medium after initial screening. After 40 days of culture, the proliferation effect is the best, the proliferation coefficient is 6.2, the average plant height is 3.4 cm, and the plant grows strongly; The aseptic buds obtained from the initial culture were transferred to the 1/2 MS + 0.4 mg/LIBA + 0.1 mg/L NAA +6.2 g/L agar + 30 g/L sucrose Rhizome medium, and after 30 days of culture, the root rate reached 96 , The number of roots per plant reached 8.2, the average root length was 3.6 cm, and the root growth was the best.},
 year = {2020}
}

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TY  - JOUR
T1  - Effects of Concentrations of IBA, NAA and 6-BA on the Proliferation and Rooting Culture of Chamomile
AU  - Bai Yanrong
AU  - Wang Jinying
Y1  - 2020/07/28
PY  - 2020
T2  - Asia-Pacific Journal of Biology
JF  - Asia-Pacific Journal of Biology
JO  - Asia-Pacific Journal of Biology
SP  - 1
EP  - 6
PB  - Science Publishing Group
UR  - http://www.sciencepg.com/article/10046032
AB  - Objective: To select the best culture medium for proliferation and root culture. Method: Using aseptic buds obtained from the first generation of culture as the experimental material, different concentrations of auxin (IBA, NAA) and cytokinin 6-BA were combined to conduct proliferation culture and rooting culture of Chamomile. Result: The results showed that the aseptic buds of primary culture were transferred to MS +1.3 mg/L6-BA +0.2 mg LIBA +6.2 g/L agarose +30 g/L sucrose + 0.1 g/L activated carbon culture medium after initial screening. After 40 days of culture, the proliferation effect is the best, the proliferation coefficient is 6.2, the average plant height is 3.4 cm, and the plant grows strongly; The aseptic buds obtained from the initial culture were transferred to the 1/2 MS + 0.4 mg/LIBA + 0.1 mg/L NAA +6.2 g/L agar + 30 g/L sucrose Rhizome medium, and after 30 days of culture, the root rate reached 96 , The number of roots per plant reached 8.2, the average root length was 3.6 cm, and the root growth was the best.
VL  - 3
IS  - 1
ER  -

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Author Information

Bai Yanrong

College of Agronomy and Life Sciences, Kunming University, Kun Ming, China
Wang Jinying

School of Architectural Engineering and Management, Kunming University, Kunming, China

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Plain Text BibTeX RIS

APA Style

Bai Yanrong, Wang Jinying. (2020). Effects of Concentrations of IBA, NAA and 6-BA on the Proliferation and Rooting Culture of Chamomile. Asia-Pacific Journal of Biology, 3(1), 1-6.

Copy | Download

ACS Style

Bai Yanrong; Wang Jinying. Effects of Concentrations of IBA, NAA and 6-BA on the Proliferation and Rooting Culture of Chamomile. Asia-Pac. J. Biol. 2020, 3(1), 1-6.

Copy | Download

AMA Style

Bai Yanrong, Wang Jinying. Effects of Concentrations of IBA, NAA and 6-BA on the Proliferation and Rooting Culture of Chamomile. Asia-Pac J Biol. 2020;3(1):1-6.

Copy | Download

@article{10046032,
  author = {Bai Yanrong and Wang Jinying},
  title = {Effects of Concentrations of IBA, NAA and 6-BA on the Proliferation and Rooting Culture of Chamomile},
  journal = {Asia-Pacific Journal of Biology},
  volume = {3},
  number = {1},
  pages = {1-6},
  url = {https://www.sciencepublishinggroup.com/article/10046032},
  abstract = {Objective: To select the best culture medium for proliferation and root culture. Method: Using aseptic buds obtained from the first generation of culture as the experimental material, different concentrations of auxin (IBA, NAA) and cytokinin 6-BA were combined to conduct proliferation culture and rooting culture of Chamomile. Result: The results showed that the aseptic buds of primary culture were transferred to MS +1.3 mg/L6-BA +0.2 mg LIBA +6.2 g/L agarose +30 g/L sucrose + 0.1 g/L activated carbon culture medium after initial screening. After 40 days of culture, the proliferation effect is the best, the proliferation coefficient is 6.2, the average plant height is 3.4 cm, and the plant grows strongly; The aseptic buds obtained from the initial culture were transferred to the 1/2 MS + 0.4 mg/LIBA + 0.1 mg/L NAA +6.2 g/L agar + 30 g/L sucrose Rhizome medium, and after 30 days of culture, the root rate reached 96 , The number of roots per plant reached 8.2, the average root length was 3.6 cm, and the root growth was the best.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Effects of Concentrations of IBA, NAA and 6-BA on the Proliferation and Rooting Culture of Chamomile
AU  - Bai Yanrong
AU  - Wang Jinying
Y1  - 2020/07/28
PY  - 2020
T2  - Asia-Pacific Journal of Biology
JF  - Asia-Pacific Journal of Biology
JO  - Asia-Pacific Journal of Biology
SP  - 1
EP  - 6
PB  - Science Publishing Group
UR  - http://www.sciencepg.com/article/10046032
AB  - Objective: To select the best culture medium for proliferation and root culture. Method: Using aseptic buds obtained from the first generation of culture as the experimental material, different concentrations of auxin (IBA, NAA) and cytokinin 6-BA were combined to conduct proliferation culture and rooting culture of Chamomile. Result: The results showed that the aseptic buds of primary culture were transferred to MS +1.3 mg/L6-BA +0.2 mg LIBA +6.2 g/L agarose +30 g/L sucrose + 0.1 g/L activated carbon culture medium after initial screening. After 40 days of culture, the proliferation effect is the best, the proliferation coefficient is 6.2, the average plant height is 3.4 cm, and the plant grows strongly; The aseptic buds obtained from the initial culture were transferred to the 1/2 MS + 0.4 mg/LIBA + 0.1 mg/L NAA +6.2 g/L agar + 30 g/L sucrose Rhizome medium, and after 30 days of culture, the root rate reached 96 , The number of roots per plant reached 8.2, the average root length was 3.6 cm, and the root growth was the best.
VL  - 3
IS  - 1
ER  -

Copy | Download