A Method of “Scope” PCR Abbreviated by Self-Complementation of Primers 5'end

Jiang Hong; Qu Yue; Qu Chaoqi

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A Method of “Scope” PCR Abbreviated by Self-Complementation of Primers 5'end

Jiang Hong, Qu Yue, Qu Chaoqi

Published in Asia-Pacific Journal of Life Sciences (Volume 1, Issue 1)

Received: 4 March 2019 Accepted: 16 April 2019 Published: 30 April 2019

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Abstract

Scope (-fluorescent PCR) is the abbreviation of self-complementation of primers 5'end. A pair of 5'ends of primers with a central homologous bases is preceded by a complementary sequence with the 5' ends of the opposite primer, and a restriction endonuclease sequence is formed between the two. Now the new 5' ends of primer pairs with two partial homology segments is complemented to each other. So the 3'terminal complementation of the amplified products can also be template and mutually primer, and amplified by＞2ⁿ index or to compensate for the consumption of primers. The sensitivity of Scope fluorescent PCR can be increased by 5.5 cycles in parallel, and there is no primer-dimer non-specificity because of the two homology segments.

Published in	Asia-Pacific Journal of Life Sciences (Volume 1, Issue 1)
Page(s)	1-4
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Fluorescent PCR, Primer-Dimer, Middle Homo-Sequence Primer

References

[1]	美国专利：1987, US patent: 4,683,202.
[2]	Wittwer C.,et al,1997,BioTechniques. ,Vol:22, p30- 138.
[3]	Vogelstein B.,et al,1999,Digital PCR.,PNAS USA Vol:96,p9236-9241.
[4]	Brownie J.,et al,1997, Nucleic Acids Res. Vol.25( No16),p235-3241.
[5]	姜文灿等，2017年5月，临床检验杂志，第35卷第5期，第370-372页。
[6]	美国专利：2018, Patent No US:10,036,062B2.
[7]	Kutyavin IV,2010,Nucleic Acids Res. , Vol: 38(5),e29, p1-12.
[8]	Longo,M.C., et al.,1990,Gene., Vol: 93, p125-128.
[9]	Porter-Jordan k; et al., 1990, J.Med Virol 30(2):85-91.
[10]	Sykes, P.J.; et al., 1992, BioTechniques 13:444-449.

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Jiang Hong, Qu Yue, Qu Chaoqi. (2019). A Method of “Scope” PCR Abbreviated by Self-Complementation of Primers 5'end. Asia-Pacific Journal of Life Sciences, 1(1), 1-4.

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ACS Style

Jiang Hong; Qu Yue; Qu Chaoqi. A Method of “Scope” PCR Abbreviated by Self-Complementation of Primers 5'end. Asia-Pac. J. Life Sci. 2019, 1(1), 1-4.

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AMA Style

Jiang Hong, Qu Yue, Qu Chaoqi. A Method of “Scope” PCR Abbreviated by Self-Complementation of Primers 5'end. Asia-Pac J Life Sci. 2019;1(1):1-4.

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@article{10037976,
  author = {Jiang Hong and Qu Yue and Qu Chaoqi},
  title = {A Method of “Scope” PCR Abbreviated by Self-Complementation of Primers 5'end},
  journal = {Asia-Pacific Journal of Life Sciences},
  volume = {1},
  number = {1},
  pages = {1-4},
  url = {https://www.sciencepublishinggroup.com/article/10037976},
  abstract = {Scope (-fluorescent PCR) is the abbreviation of self-complementation of primers 5'end. A pair of 5'ends of primers with a central homologous bases is preceded by a complementary sequence with the 5' ends of the opposite primer, and a restriction endonuclease sequence is formed between the two. Now the new 5' ends of primer pairs with two partial homology segments is complemented to each other. So the 3'terminal complementation of the amplified products can also be template and mutually primer, and amplified by＞2n index or to compensate for the consumption of primers. The sensitivity of Scope fluorescent PCR can be increased by 5.5 cycles in parallel, and there is no primer-dimer non-specificity because of the two homology segments.},
 year = {2019}
}

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TY  - JOUR
T1  - A Method of “Scope” PCR Abbreviated by Self-Complementation of Primers 5'end
AU  - Jiang Hong
AU  - Qu Yue
AU  - Qu Chaoqi
Y1  - 2019/04/30
PY  - 2019
T2  - Asia-Pacific Journal of Life Sciences
JF  - Asia-Pacific Journal of Life Sciences
JO  - Asia-Pacific Journal of Life Sciences
SP  - 1
EP  - 4
PB  - Science Publishing Group
UR  - http://www.sciencepg.com/article/10037976
AB  - Scope (-fluorescent PCR) is the abbreviation of self-complementation of primers 5'end. A pair of 5'ends of primers with a central homologous bases is preceded by a complementary sequence with the 5' ends of the opposite primer, and a restriction endonuclease sequence is formed between the two. Now the new 5' ends of primer pairs with two partial homology segments is complemented to each other. So the 3'terminal complementation of the amplified products can also be template and mutually primer, and amplified by＞2n index or to compensate for the consumption of primers. The sensitivity of Scope fluorescent PCR can be increased by 5.5 cycles in parallel, and there is no primer-dimer non-specificity because of the two homology segments.
VL  - 1
IS  - 1
ER  -

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Author Information

Jiang Hong

Digen Digital Gene Co., Ltd., Macau, P.R.China; Beijing TagArray Molecular Testing Co., Ltd., Beijing, P.R.China
Qu Yue

Digen Digital Gene Co., Ltd., Macau, P.R.China
Qu Chaoqi

Digen Digital Gene Co., Ltd., Macau, P.R.China

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Plain Text BibTeX RIS

APA Style

Jiang Hong, Qu Yue, Qu Chaoqi. (2019). A Method of “Scope” PCR Abbreviated by Self-Complementation of Primers 5'end. Asia-Pacific Journal of Life Sciences, 1(1), 1-4.

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ACS Style

Jiang Hong; Qu Yue; Qu Chaoqi. A Method of “Scope” PCR Abbreviated by Self-Complementation of Primers 5'end. Asia-Pac. J. Life Sci. 2019, 1(1), 1-4.

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AMA Style

Jiang Hong, Qu Yue, Qu Chaoqi. A Method of “Scope” PCR Abbreviated by Self-Complementation of Primers 5'end. Asia-Pac J Life Sci. 2019;1(1):1-4.

Copy | Download

@article{10037976,
  author = {Jiang Hong and Qu Yue and Qu Chaoqi},
  title = {A Method of “Scope” PCR Abbreviated by Self-Complementation of Primers 5'end},
  journal = {Asia-Pacific Journal of Life Sciences},
  volume = {1},
  number = {1},
  pages = {1-4},
  url = {https://www.sciencepublishinggroup.com/article/10037976},
  abstract = {Scope (-fluorescent PCR) is the abbreviation of self-complementation of primers 5'end. A pair of 5'ends of primers with a central homologous bases is preceded by a complementary sequence with the 5' ends of the opposite primer, and a restriction endonuclease sequence is formed between the two. Now the new 5' ends of primer pairs with two partial homology segments is complemented to each other. So the 3'terminal complementation of the amplified products can also be template and mutually primer, and amplified by＞2n index or to compensate for the consumption of primers. The sensitivity of Scope fluorescent PCR can be increased by 5.5 cycles in parallel, and there is no primer-dimer non-specificity because of the two homology segments.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - A Method of “Scope” PCR Abbreviated by Self-Complementation of Primers 5'end
AU  - Jiang Hong
AU  - Qu Yue
AU  - Qu Chaoqi
Y1  - 2019/04/30
PY  - 2019
T2  - Asia-Pacific Journal of Life Sciences
JF  - Asia-Pacific Journal of Life Sciences
JO  - Asia-Pacific Journal of Life Sciences
SP  - 1
EP  - 4
PB  - Science Publishing Group
UR  - http://www.sciencepg.com/article/10037976
AB  - Scope (-fluorescent PCR) is the abbreviation of self-complementation of primers 5'end. A pair of 5'ends of primers with a central homologous bases is preceded by a complementary sequence with the 5' ends of the opposite primer, and a restriction endonuclease sequence is formed between the two. Now the new 5' ends of primer pairs with two partial homology segments is complemented to each other. So the 3'terminal complementation of the amplified products can also be template and mutually primer, and amplified by＞2n index or to compensate for the consumption of primers. The sensitivity of Scope fluorescent PCR can be increased by 5.5 cycles in parallel, and there is no primer-dimer non-specificity because of the two homology segments.
VL  - 1
IS  - 1
ER  -

Copy | Download