Department of Plant Protection, Agricultural and Natural Resources Research and Education Center,
Nematotoxicity of three nanoparticles I.e. Silver (AgNP, 20 nm), Silicon oxide (SiO₂NP, 11-14 nm) and Titanium oxide (TiO₂NP, 20 nm) on root knot nematode, Meloidogyne incognita (Kofoid and White) Chitwood, were studied as laboratory and pot experiments during 2011-2012. Application rates of all nanoparticles were ranged as 1.5, 3, 6, 12.5, 25, 50, 100, 200, 400 and 800 mg nanoparticles/ml water dissolved in water media for laboratory experiment to see their effects on motility and mortality of second stage juveniles (J2s) of M. incognita. In pot experiment, 0.02, 0.01, 0.005, 0.025, 0.0125 and 0.0007 % (w/w) of nano-silver and nano-Titanium were applied and examined on nematode activity and plant growth components of tomato (Solanum lycopersicum L.). Results showed 100% J2 immobility and mortality in treatments of 800, 400 and 200 mg/ml of AgNP. LC50 for AgNP was treatment of 100 mg/ml. J2 mortality was recorded as 48, 16 and 7.3 per cent in treatments of 100, 50 and 25 mg/ml of AgNP which were 4.3 and 2 per cent in treatments of 800 and 400 mg/ml of TiO₂NP respectively. No J2 mortality was seen in all other treatments after 24 h exposing time. In pot experiment, all treatments of AgNP and treatment of TiO2NP 0.02% completely controlled nematode. Treatments of 0.02, 0.01 and 0.005% of AgNP as well as 0/02, 0.01% of TiO₂NP were highly toxic to the tomato and significantly (P=0.01) reduced tomato root and stem length and fresh weights in comparison to control.