The aim of this study was to test the in vitro toxicity of an extract of Strychnos johnsonii (Loganiaceae) on a strain of Staphylococcus aureus. The tests were performed in the bacteriology unit of the Douala General Hospital biology laboratory, dealing with an extract from the bark of stem of Strychnos johnsonii (Loganiaceae) harvested at Etome village, South West Cameroon and authenticated by a botanist. The plant extract was obtained by maceration in 300 mL of ethanol for 120 hours. The filtrate obtained was evaporated under vacuum, at 50° C, 250 mbar of pressure and at a speed of 125 rounds per minute. The residual solvent was eliminated in an incubator at 37° C for one week to give dry extract. Selected bacterial strain came from pus collected from an in-patient. By its biochemical and enzymatic characters, this strain showed 90.9% homology with the Staphylococcus aureus ATCC 29213-reference strain. No bacterial growth were observed on Mannitol Salt, EMB and Sabouraud-Chloramphenicol agar plates after 48 hours of incubation, evidence that the extract contained no germs before the test. The number of initial colonies for the time t0 averaged 225. The point of intersection between the inhibition curve and the x-axis as the MIC corresponds to 0,04 g/mL. The smallest concentration of the extract for which the growth of Staphylococcus aureus is zero on the Mannitol salt agar was 0.04 g/mL. Therefore, the MIC amounts to MBC. The results obtained showed a bactericidal effect, which could be, attributed to the presence of indole alkaloids in the plant.
| DOI | 10.11648/j.jdmp.20150103.12 |
| Published in | Journal of Diseases and Medicinal Plants (Volume 1, Issue 3, August 2015) |
| Page(s) | 48-52 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Strychnos johnsonii, In Vitro Toxicity, Staphylococcus aureus
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APA Style
Cecile Okalla Ebongue, Fanny Aimee Essombe Malolo, François Eya Ane Meva, Lidwine Ngah, Jean Claude Ndom, et al. (2015). In Vitro Toxicity Test of Strichnos johnsonnii (Loganiaceae) on a Strain of Staphylococcus aureus. Journal of Diseases and Medicinal Plants, 1(3), 48-52. https://doi.org/10.11648/j.jdmp.20150103.12
ACS Style
Cecile Okalla Ebongue; Fanny Aimee Essombe Malolo; François Eya Ane Meva; Lidwine Ngah; Jean Claude Ndom, et al. In Vitro Toxicity Test of Strichnos johnsonnii (Loganiaceae) on a Strain of Staphylococcus aureus. J. Dis. Med. Plants 2015, 1(3), 48-52. doi: 10.11648/j.jdmp.20150103.12
AMA Style
Cecile Okalla Ebongue, Fanny Aimee Essombe Malolo, François Eya Ane Meva, Lidwine Ngah, Jean Claude Ndom, et al. In Vitro Toxicity Test of Strichnos johnsonnii (Loganiaceae) on a Strain of Staphylococcus aureus. J Dis Med Plants. 2015;1(3):48-52. doi: 10.11648/j.jdmp.20150103.12
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Download@article{10.11648/j.jdmp.20150103.12,
author = {Cecile Okalla Ebongue and Fanny Aimee Essombe Malolo and François Eya Ane Meva and Lidwine Ngah and Jean Claude Ndom and Emmanuel Mpondo Mpondo},
title = {In Vitro Toxicity Test of Strichnos johnsonnii (Loganiaceae) on a Strain of Staphylococcus aureus},
journal = {Journal of Diseases and Medicinal Plants},
volume = {1},
number = {3},
pages = {48-52},
doi = {10.11648/j.jdmp.20150103.12},
url = {https://doi.org/10.11648/j.jdmp.20150103.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20150103.12},
abstract = {The aim of this study was to test the in vitro toxicity of an extract of Strychnos johnsonii (Loganiaceae) on a strain of Staphylococcus aureus. The tests were performed in the bacteriology unit of the Douala General Hospital biology laboratory, dealing with an extract from the bark of stem of Strychnos johnsonii (Loganiaceae) harvested at Etome village, South West Cameroon and authenticated by a botanist. The plant extract was obtained by maceration in 300 mL of ethanol for 120 hours. The filtrate obtained was evaporated under vacuum, at 50° C, 250 mbar of pressure and at a speed of 125 rounds per minute. The residual solvent was eliminated in an incubator at 37° C for one week to give dry extract. Selected bacterial strain came from pus collected from an in-patient. By its biochemical and enzymatic characters, this strain showed 90.9% homology with the Staphylococcus aureus ATCC 29213-reference strain. No bacterial growth were observed on Mannitol Salt, EMB and Sabouraud-Chloramphenicol agar plates after 48 hours of incubation, evidence that the extract contained no germs before the test. The number of initial colonies for the time t0 averaged 225. The point of intersection between the inhibition curve and the x-axis as the MIC corresponds to 0,04 g/mL. The smallest concentration of the extract for which the growth of Staphylococcus aureus is zero on the Mannitol salt agar was 0.04 g/mL. Therefore, the MIC amounts to MBC. The results obtained showed a bactericidal effect, which could be, attributed to the presence of indole alkaloids in the plant.},
year = {2015}
}
TY - JOUR T1 - In Vitro Toxicity Test of Strichnos johnsonnii (Loganiaceae) on a Strain of Staphylococcus aureus AU - Cecile Okalla Ebongue AU - Fanny Aimee Essombe Malolo AU - François Eya Ane Meva AU - Lidwine Ngah AU - Jean Claude Ndom AU - Emmanuel Mpondo Mpondo Y1 - 2015/07/01 PY - 2015 N1 - https://doi.org/10.11648/j.jdmp.20150103.12 DO - 10.11648/j.jdmp.20150103.12 T2 - Journal of Diseases and Medicinal Plants JF - Journal of Diseases and Medicinal Plants JO - Journal of Diseases and Medicinal Plants SP - 48 EP - 52 PB - Science Publishing Group SN - 2469-8210 UR - https://doi.org/10.11648/j.jdmp.20150103.12 AB - The aim of this study was to test the in vitro toxicity of an extract of Strychnos johnsonii (Loganiaceae) on a strain of Staphylococcus aureus. The tests were performed in the bacteriology unit of the Douala General Hospital biology laboratory, dealing with an extract from the bark of stem of Strychnos johnsonii (Loganiaceae) harvested at Etome village, South West Cameroon and authenticated by a botanist. The plant extract was obtained by maceration in 300 mL of ethanol for 120 hours. The filtrate obtained was evaporated under vacuum, at 50° C, 250 mbar of pressure and at a speed of 125 rounds per minute. The residual solvent was eliminated in an incubator at 37° C for one week to give dry extract. Selected bacterial strain came from pus collected from an in-patient. By its biochemical and enzymatic characters, this strain showed 90.9% homology with the Staphylococcus aureus ATCC 29213-reference strain. No bacterial growth were observed on Mannitol Salt, EMB and Sabouraud-Chloramphenicol agar plates after 48 hours of incubation, evidence that the extract contained no germs before the test. The number of initial colonies for the time t0 averaged 225. The point of intersection between the inhibition curve and the x-axis as the MIC corresponds to 0,04 g/mL. The smallest concentration of the extract for which the growth of Staphylococcus aureus is zero on the Mannitol salt agar was 0.04 g/mL. Therefore, the MIC amounts to MBC. The results obtained showed a bactericidal effect, which could be, attributed to the presence of indole alkaloids in the plant. VL - 1 IS - 3 ER -
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