A molecular research was carried out in the areas of Lahore in order to confirm the existence of B. abortus antigen in Caprine and ovine by utilizing molecular techniques in this study. Small ruminants can be infected with B. abortus (the causative agent of bovine brucellosis), which complicates brucellosis control efforts because most brucellosis control programmes rely on immunological testing rather than genomic testing to determine the specific species circulating in ruminants. Now at this investigation, n = 1270 goat serum samples and n = 770 sheep serum samples were collected, respectively. After already being tested with the Rose Bengal test, all positive specimens were examined to the real-time PCR technique. RBT confirmed brucellosis prevalence of 21.43±0.37% and 18.11±0.12 in caprine and ovine respectively. Out of 230 positive goat samples, real-time PCR found B. abortus in 150 samples (65.21±0.51) in sheep and goats and 118 samples (71.51±0.21%) out of 165 seropositive sheep samples. Brucella abortus infection in small ruminants could be caused by a combination of factors including mixed farming of small and large ruminants, sharing of the same pasture, and the presence of reservoir hosts on a farm, all of which could be risk factors for Brucella species cross-infection in non-species hosts. It has been determined that B. abortus is the causative agent of caprine and ovine brucellosis in the country of Pakistan. Results of this study can be utilised to develop successful brucellosis eradication and control strategies in small ruminants, which can be applied to other animals.
| Published in | Animal and Veterinary Sciences (Volume 9, Issue 5) |
| DOI | 10.11648/j.avs.20210905.13 |
| Page(s) | 141-144 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Brucella Abortus, Sheep, Goat, Real-time PCR, RBPT
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APA Style
Muhammad Bilawal Arain, Abdullah Babar, Muhammad Ibrahim Panhwar, Khush Hal, Muhammad Mubashir Farooq, et al. (2021). Detection of Brucella Abortus in Caprine and Ovine by Real-Time PCR Assay. Animal and Veterinary Sciences, 9(5), 141-144. https://doi.org/10.11648/j.avs.20210905.13
ACS Style
Muhammad Bilawal Arain; Abdullah Babar; Muhammad Ibrahim Panhwar; Khush Hal; Muhammad Mubashir Farooq, et al. Detection of Brucella Abortus in Caprine and Ovine by Real-Time PCR Assay. Anim. Vet. Sci. 2021, 9(5), 141-144. doi: 10.11648/j.avs.20210905.13
AMA Style
Muhammad Bilawal Arain, Abdullah Babar, Muhammad Ibrahim Panhwar, Khush Hal, Muhammad Mubashir Farooq, et al. Detection of Brucella Abortus in Caprine and Ovine by Real-Time PCR Assay. Anim Vet Sci. 2021;9(5):141-144. doi: 10.11648/j.avs.20210905.13
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Download@article{10.11648/j.avs.20210905.13,
author = {Muhammad Bilawal Arain and Abdullah Babar and Muhammad Ibrahim Panhwar and Khush Hal and Muhammad Mubashir Farooq and Zainab Lanjar and Ali Gul Soomro and Saqib Ali Fazilani and Muhammad Naeem Jan and Loveson Lakhani and Adnan Yousaf and Inayatullah Sarki and Rehana Shahnawaz and Mathan},
title = {Detection of Brucella Abortus in Caprine and Ovine by Real-Time PCR Assay},
journal = {Animal and Veterinary Sciences},
volume = {9},
number = {5},
pages = {141-144},
doi = {10.11648/j.avs.20210905.13},
url = {https://doi.org/10.11648/j.avs.20210905.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20210905.13},
abstract = {A molecular research was carried out in the areas of Lahore in order to confirm the existence of B. abortus antigen in Caprine and ovine by utilizing molecular techniques in this study. Small ruminants can be infected with B. abortus (the causative agent of bovine brucellosis), which complicates brucellosis control efforts because most brucellosis control programmes rely on immunological testing rather than genomic testing to determine the specific species circulating in ruminants. Now at this investigation, n = 1270 goat serum samples and n = 770 sheep serum samples were collected, respectively. After already being tested with the Rose Bengal test, all positive specimens were examined to the real-time PCR technique. RBT confirmed brucellosis prevalence of 21.43±0.37% and 18.11±0.12 in caprine and ovine respectively. Out of 230 positive goat samples, real-time PCR found B. abortus in 150 samples (65.21±0.51) in sheep and goats and 118 samples (71.51±0.21%) out of 165 seropositive sheep samples. Brucella abortus infection in small ruminants could be caused by a combination of factors including mixed farming of small and large ruminants, sharing of the same pasture, and the presence of reservoir hosts on a farm, all of which could be risk factors for Brucella species cross-infection in non-species hosts. It has been determined that B. abortus is the causative agent of caprine and ovine brucellosis in the country of Pakistan. Results of this study can be utilised to develop successful brucellosis eradication and control strategies in small ruminants, which can be applied to other animals.},
year = {2021}
}
TY - JOUR T1 - Detection of Brucella Abortus in Caprine and Ovine by Real-Time PCR Assay AU - Muhammad Bilawal Arain AU - Abdullah Babar AU - Muhammad Ibrahim Panhwar AU - Khush Hal AU - Muhammad Mubashir Farooq AU - Zainab Lanjar AU - Ali Gul Soomro AU - Saqib Ali Fazilani AU - Muhammad Naeem Jan AU - Loveson Lakhani AU - Adnan Yousaf AU - Inayatullah Sarki AU - Rehana Shahnawaz AU - Mathan Y1 - 2021/10/21 PY - 2021 N1 - https://doi.org/10.11648/j.avs.20210905.13 DO - 10.11648/j.avs.20210905.13 T2 - Animal and Veterinary Sciences JF - Animal and Veterinary Sciences JO - Animal and Veterinary Sciences SP - 141 EP - 144 PB - Science Publishing Group SN - 2328-5850 UR - https://doi.org/10.11648/j.avs.20210905.13 AB - A molecular research was carried out in the areas of Lahore in order to confirm the existence of B. abortus antigen in Caprine and ovine by utilizing molecular techniques in this study. Small ruminants can be infected with B. abortus (the causative agent of bovine brucellosis), which complicates brucellosis control efforts because most brucellosis control programmes rely on immunological testing rather than genomic testing to determine the specific species circulating in ruminants. Now at this investigation, n = 1270 goat serum samples and n = 770 sheep serum samples were collected, respectively. After already being tested with the Rose Bengal test, all positive specimens were examined to the real-time PCR technique. RBT confirmed brucellosis prevalence of 21.43±0.37% and 18.11±0.12 in caprine and ovine respectively. Out of 230 positive goat samples, real-time PCR found B. abortus in 150 samples (65.21±0.51) in sheep and goats and 118 samples (71.51±0.21%) out of 165 seropositive sheep samples. Brucella abortus infection in small ruminants could be caused by a combination of factors including mixed farming of small and large ruminants, sharing of the same pasture, and the presence of reservoir hosts on a farm, all of which could be risk factors for Brucella species cross-infection in non-species hosts. It has been determined that B. abortus is the causative agent of caprine and ovine brucellosis in the country of Pakistan. Results of this study can be utilised to develop successful brucellosis eradication and control strategies in small ruminants, which can be applied to other animals. VL - 9 IS - 5 ER -
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