Preparation and Antioxidant Activities of New Di-Amino-Schiff Base Derivatives of Chitosan

Zhimei Zhong; Guangnan Zhu; Zhihong Zhao; Haijie Li; Zhenting Zhang

doi:doi:10.11648/j.bio.20210906.13

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Preparation and Antioxidant Activities of New Di-Amino-Schiff Base Derivatives of Chitosan

Zhimei Zhong, Guangnan Zhu, Zhihong Zhao, Haijie Li, Zhenting Zhang

Published in American Journal of Bioscience and Bioengineering (Volume 9, Issue 6)

Received: 18 October 2021 Accepted: 24 November 2021 Published: 2 December 2021

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Abstract

The damage caused by reactive oxygen radicals to the body is huge, and it can lead to aging of the human body and cause the occurrence of many diseases. Therefore, finding new antioxidant substance is urgent. In order to obtain new non-toxic antioxidant reagent, 3 kinds of di-amino-Schiff base derivatives of chitosan were prepared by grafting urea molecule onto chitosan chain using chitosan as raw material in this paper. The reaction conditions such as different reaction time, temperature, solvent and the molar ratio of reaction materials were discussed, and the structures of the derivatives were characterized by FT-IR and ¹³C NMR spectroscopy and elemental analysis. The antioxidant activities of chitosan and its derivatives were tested, including the scavenging ability of superoxide anions, hydroxyl radicals and DPPH radicals. The experimental results show that the derivatives and chitosan all have good antioxidant activities, of which the removal rate of HCS for ·OH is 78.2%; and the clearance of DSABHCS at concentration of 500 μg·mL^-1 toward O₂^- is 97.8%; the clearance of LCS to DPPH is 94.3% at concentration of 600 μg·mL^-1. These results laid the foundation for the development of chitosan and its derivatives using as new antioxidant reagents.

Published in	American Journal of Bioscience and Bioengineering (Volume 9, Issue 6)
DOI	10.11648/j.bio.20210906.13
Page(s)	162-168
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Chitosan, Urea, Derivatives, Preparation, Antioxidant Activity

References

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APA Style

Zhimei Zhong, Guangnan Zhu, Zhihong Zhao, Haijie Li, Zhenting Zhang. (2021). Preparation and Antioxidant Activities of New Di-Amino-Schiff Base Derivatives of Chitosan. American Journal of Bioscience and Bioengineering, 9(6), 162-168. https://doi.org/10.11648/j.bio.20210906.13

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ACS Style

Zhimei Zhong; Guangnan Zhu; Zhihong Zhao; Haijie Li; Zhenting Zhang. Preparation and Antioxidant Activities of New Di-Amino-Schiff Base Derivatives of Chitosan. Am. J. BioSci. Bioeng. 2021, 9(6), 162-168. doi: 10.11648/j.bio.20210906.13

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AMA Style

Zhimei Zhong, Guangnan Zhu, Zhihong Zhao, Haijie Li, Zhenting Zhang. Preparation and Antioxidant Activities of New Di-Amino-Schiff Base Derivatives of Chitosan. Am J BioSci Bioeng. 2021;9(6):162-168. doi: 10.11648/j.bio.20210906.13

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@article{10.11648/j.bio.20210906.13,
  author = {Zhimei Zhong and Guangnan Zhu and Zhihong Zhao and Haijie Li and Zhenting Zhang},
  title = {Preparation and Antioxidant Activities of New Di-Amino-Schiff Base Derivatives of Chitosan},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {9},
  number = {6},
  pages = {162-168},
  doi = {10.11648/j.bio.20210906.13},
  url = {https://doi.org/10.11648/j.bio.20210906.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20210906.13},
  abstract = {The damage caused by reactive oxygen radicals to the body is huge, and it can lead to aging of the human body and cause the occurrence of many diseases. Therefore, finding new antioxidant substance is urgent. In order to obtain new non-toxic antioxidant reagent, 3 kinds of di-amino-Schiff base derivatives of chitosan were prepared by grafting urea molecule onto chitosan chain using chitosan as raw material in this paper. The reaction conditions such as different reaction time, temperature, solvent and the molar ratio of reaction materials were discussed, and the structures of the derivatives were characterized by FT-IR and 13C NMR spectroscopy and elemental analysis. The antioxidant activities of chitosan and its derivatives were tested, including the scavenging ability of superoxide anions, hydroxyl radicals and DPPH radicals. The experimental results show that the derivatives and chitosan all have good antioxidant activities, of which the removal rate of HCS for ·OH is 78.2%; and the clearance of DSABHCS at concentration of 500 μg·mL-1 toward O2- is 97.8%; the clearance of LCS to DPPH is 94.3% at concentration of 600 μg·mL-1. These results laid the foundation for the development of chitosan and its derivatives using as new antioxidant reagents.},
 year = {2021}
}

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TY  - JOUR
T1  - Preparation and Antioxidant Activities of New Di-Amino-Schiff Base Derivatives of Chitosan
AU  - Zhimei Zhong
AU  - Guangnan Zhu
AU  - Zhihong Zhao
AU  - Haijie Li
AU  - Zhenting Zhang
Y1  - 2021/12/02
PY  - 2021
N1  - https://doi.org/10.11648/j.bio.20210906.13
DO  - 10.11648/j.bio.20210906.13
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 162
EP  - 168
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20210906.13
AB  - The damage caused by reactive oxygen radicals to the body is huge, and it can lead to aging of the human body and cause the occurrence of many diseases. Therefore, finding new antioxidant substance is urgent. In order to obtain new non-toxic antioxidant reagent, 3 kinds of di-amino-Schiff base derivatives of chitosan were prepared by grafting urea molecule onto chitosan chain using chitosan as raw material in this paper. The reaction conditions such as different reaction time, temperature, solvent and the molar ratio of reaction materials were discussed, and the structures of the derivatives were characterized by FT-IR and 13C NMR spectroscopy and elemental analysis. The antioxidant activities of chitosan and its derivatives were tested, including the scavenging ability of superoxide anions, hydroxyl radicals and DPPH radicals. The experimental results show that the derivatives and chitosan all have good antioxidant activities, of which the removal rate of HCS for ·OH is 78.2%; and the clearance of DSABHCS at concentration of 500 μg·mL-1 toward O2- is 97.8%; the clearance of LCS to DPPH is 94.3% at concentration of 600 μg·mL-1. These results laid the foundation for the development of chitosan and its derivatives using as new antioxidant reagents.
VL  - 9
IS  - 6
ER  -

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Author Information

Zhimei Zhong

College of Sciences, Inner Mongolia Agricultural University, Huhhot, China
Guangnan Zhu

College of Sciences, Inner Mongolia Agricultural University, Huhhot, China
Zhihong Zhao

College of Sciences, Inner Mongolia Agricultural University, Huhhot, China
Haijie Li

College of Sciences, Inner Mongolia Agricultural University, Huhhot, China
Zhenting Zhang

College of Sciences, Inner Mongolia Agricultural University, Huhhot, China

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APA Style

Zhimei Zhong, Guangnan Zhu, Zhihong Zhao, Haijie Li, Zhenting Zhang. (2021). Preparation and Antioxidant Activities of New Di-Amino-Schiff Base Derivatives of Chitosan. American Journal of Bioscience and Bioengineering, 9(6), 162-168. https://doi.org/10.11648/j.bio.20210906.13

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ACS Style

Zhimei Zhong; Guangnan Zhu; Zhihong Zhao; Haijie Li; Zhenting Zhang. Preparation and Antioxidant Activities of New Di-Amino-Schiff Base Derivatives of Chitosan. Am. J. BioSci. Bioeng. 2021, 9(6), 162-168. doi: 10.11648/j.bio.20210906.13

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AMA Style

Zhimei Zhong, Guangnan Zhu, Zhihong Zhao, Haijie Li, Zhenting Zhang. Preparation and Antioxidant Activities of New Di-Amino-Schiff Base Derivatives of Chitosan. Am J BioSci Bioeng. 2021;9(6):162-168. doi: 10.11648/j.bio.20210906.13

Copy | Download

@article{10.11648/j.bio.20210906.13,
  author = {Zhimei Zhong and Guangnan Zhu and Zhihong Zhao and Haijie Li and Zhenting Zhang},
  title = {Preparation and Antioxidant Activities of New Di-Amino-Schiff Base Derivatives of Chitosan},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {9},
  number = {6},
  pages = {162-168},
  doi = {10.11648/j.bio.20210906.13},
  url = {https://doi.org/10.11648/j.bio.20210906.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20210906.13},
  abstract = {The damage caused by reactive oxygen radicals to the body is huge, and it can lead to aging of the human body and cause the occurrence of many diseases. Therefore, finding new antioxidant substance is urgent. In order to obtain new non-toxic antioxidant reagent, 3 kinds of di-amino-Schiff base derivatives of chitosan were prepared by grafting urea molecule onto chitosan chain using chitosan as raw material in this paper. The reaction conditions such as different reaction time, temperature, solvent and the molar ratio of reaction materials were discussed, and the structures of the derivatives were characterized by FT-IR and 13C NMR spectroscopy and elemental analysis. The antioxidant activities of chitosan and its derivatives were tested, including the scavenging ability of superoxide anions, hydroxyl radicals and DPPH radicals. The experimental results show that the derivatives and chitosan all have good antioxidant activities, of which the removal rate of HCS for ·OH is 78.2%; and the clearance of DSABHCS at concentration of 500 μg·mL-1 toward O2- is 97.8%; the clearance of LCS to DPPH is 94.3% at concentration of 600 μg·mL-1. These results laid the foundation for the development of chitosan and its derivatives using as new antioxidant reagents.},
 year = {2021}
}

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TY  - JOUR
T1  - Preparation and Antioxidant Activities of New Di-Amino-Schiff Base Derivatives of Chitosan
AU  - Zhimei Zhong
AU  - Guangnan Zhu
AU  - Zhihong Zhao
AU  - Haijie Li
AU  - Zhenting Zhang
Y1  - 2021/12/02
PY  - 2021
N1  - https://doi.org/10.11648/j.bio.20210906.13
DO  - 10.11648/j.bio.20210906.13
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 162
EP  - 168
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20210906.13
AB  - The damage caused by reactive oxygen radicals to the body is huge, and it can lead to aging of the human body and cause the occurrence of many diseases. Therefore, finding new antioxidant substance is urgent. In order to obtain new non-toxic antioxidant reagent, 3 kinds of di-amino-Schiff base derivatives of chitosan were prepared by grafting urea molecule onto chitosan chain using chitosan as raw material in this paper. The reaction conditions such as different reaction time, temperature, solvent and the molar ratio of reaction materials were discussed, and the structures of the derivatives were characterized by FT-IR and 13C NMR spectroscopy and elemental analysis. The antioxidant activities of chitosan and its derivatives were tested, including the scavenging ability of superoxide anions, hydroxyl radicals and DPPH radicals. The experimental results show that the derivatives and chitosan all have good antioxidant activities, of which the removal rate of HCS for ·OH is 78.2%; and the clearance of DSABHCS at concentration of 500 μg·mL-1 toward O2- is 97.8%; the clearance of LCS to DPPH is 94.3% at concentration of 600 μg·mL-1. These results laid the foundation for the development of chitosan and its derivatives using as new antioxidant reagents.
VL  - 9
IS  - 6
ER  -

Copy | Download