International Journal of Economy, Energy and Environment

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Influence of Organic Amendment on Microbial Activities and Growth of Pepper Cultured on Crude Oil Contaminated Niger Delta Soil

Received: 06 May 2017    Accepted: 02 August 2017    Published: 06 September 2017
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Abstract

Land contamination with crude oil is one of the challenges of effective crop production in the Niger Delta Region of Nigeria. This condition is inherent thus creates the need for developing effective remedial methods of managing contaminated lands as well as improve crop production. This study was designed to assess the effect of Brewers Spent Grains (BSG) on microbial activities and growth of Capsicum annum L cultured in crude oil contaminated soil. The results have shown that depending on the level of contamination, crude affected both the physical and chemical attributes of soil. It increased the acidity from pH 5.67 to a range of 5.58 - 5.64 and the total organic carbon content from 0.05% to 0.14% - 0.21 while the total nitrogen and available phosphorus levels of soil were remarkably reduced from 0.39% to 0.21% - 0.28%, and from 0.036% to 0.028% - 0.032% respectively. Amendment of contaminated soil with BSG had variable influence on the microbial properties of test soil. It increases the heterotrophic activity of bacteria in soil while retarding the activities of nitrogen fixing bacteria (Nitrosomonas sp and Nitrobacter sp). However at a stimulating amendment level of 150g of BSG in a 0.208% level of contamination, the activities of hydrocarbon degrading bacteria was enhanced from the hitherto low number of 3.6 log CFU/g found in test soil to 6.2 log CFU/g obtained on the 6th week of the remediation period. This resulted in 62.09% hydrocarbon degradation in soil at the end of the 8-week remediation course. BSG amendment of the oil contaminated had clear but variable influence on some agronomic traits of the cultured pepper. Growth was generally promoted by the amendment and the most significant (P<0.05) growth attributes affected were the plant height, number of branches, leaves, flowers and fruits as well as the leaf area. Pepper seedlings exposed to 0.624% of oil and remedied with 300g (1.5%) had the highest number of flowers but the flower did develop into fruits. However plant exposed to 0.416% of oil contamination and remedied with 0.75% of BSG produced flowers that formed fruits. Although the cultured pepper plants exhibited necrotic spots, this study has shown that plants grow in crude oil contaminated soil if alternative source of nutrients is provided. This tend to grow more in lightly contaminated soil and remediation using BSG has proven to enhance the activities of microorganisms and subsequent degradation of hydrocarbons in contaminated soil. It is highly recommended for use as soil conditioner to "unmask" nutrients in contaminated soils.

DOI 10.11648/j.ijeee.20170204.12
Published in International Journal of Economy, Energy and Environment (Volume 2, Issue 4, August 2017)
Page(s) 56-76
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Contaminated Land, Organic Amendment, Crude Oil and Microbial Activities

References
[1] Abioye, O. P., Agamuthu, P., and Abdul Aziz, A. R (2012) ‘Biodegradation of used motor oil in soil using organic waste amendments’ journal of Biotechnology Research International Vol. 2012 pp. 1-8.
[2] Abu, G. O and Dike O. P (2008) ‘A study of natural attenuation processes involved in a microcosm model of crude oil-impacted wetland sediment in Niger Delta’ Bioresource Technology. Vol 99 pp. 4761-4767.
[3] Adekunle, M. I., (2011) ‘Bioremediation of soils contaminated with Nigerian petroleum products using composted municipal wastes’ bioremediation journal Vol 15 (4) pp. 230-241.
[4] Adedokun, O. M. and Ataga, A. E. (2007). Effects of amendments and bioaugumentation of soil polluted with crude oil, automotive gasoline oil, and spent engine oil on the growth of cowpea (Vigna ungiculata Walp L.). Scientific Research and Essay, 2 (5): 147-149.
[5] Adesodun, J. K., & Mbagwu J. S. (2008) ‘Biodegradation of waste lubricating petroleum oil in a tropical alfisol as mediated by animal droppings’ Bioresource Technology Vol. 85 (13) pp. 5659-5665.
[6] Adesua Teresa Ojeamiren (2014) A Comparative Analysis of Diesel Contaminated Soils Bioremediation with Brewery Spent Grain and Paper Waste. Unpublished MSc Thesis, Department of Environment, Health and Safety, Faculty of Applied Sciences, University of Sunderland, United Kingdom.
[7] Agamuthu, P., and Dadrasnia, A. (2013) ‘Potential of biowastes to remediate diesel fuel contaminated soil’ Journal of Global nest Vol 15 (4) pp. 474-484.
[8] Agbogidi, O. M., Eruotor, P. G., Akparobi, S. O. and Nnaji, G. U. (2007). Evaluation of crude oil contaminated soil on the mineral nutrient elements of maize (Zea mays L.). J. Agron., 6(1): 188-193.
[9] Akoachere, J. T. K., Akenji, T. N., Yongabi, F. N., Nkwelang, G. and Ndip, R. N (2008). Lubricating oil degrading bacteria in soils from filling stations and auto-mechanic workshops in Buea, Cameroon: occurrence and characteristics of isolates. African. J. Biotechnol. 7, 1700-1706.
[10] AOAC (2005) Association of Official Analytical Chemist, Official Methods of Analysis 18th edition Washington D. C. USA.
[11] Atlas, R. M., and Bartha, R., (2006). “Fate and effects of polluting petroleum in the marine environment”. Residue Rev. 49 (1): 49-83.
[12] Aweke Kebede and Taddese Wondimu (2004) Distribution of Trace Elements in Muscle and Organs of Tilapia, Oreochromis Niloticus, from lakes Awassa and Ziway, Ethiopia, Bull. Chem. Soc. Ethiop. 2004, 18 (2), 119-130.
[13] Ayotamuno. M. J., Kogbara R. B., Ogaji S. O. T., Probert, S. D., (2006) ‘Bioremediation of a crude-oil polluted agricultural-soil at Portharcourt, Nigeria.’ Journal of Applied Energy. Vol 83 (11) pp 1249-1257.
[14] Banwo, O. O., (2013) ‘Critical evaluation of the use of brewery spent grain in bioremediation’. Msc Dissertation, University of Sunderland.
[15] Bradi, L., Mattei, A., Steffan, S., and Marzona, M., (2000). “Hydrocarbon degradation by a soil microbial population with β-cyclodeztrin as surfactant to enhance bioavailability”. Enzyme and Microbial Technology: 27 (3); 709-713.
[16] Cheesbrough, M., 2006. District Laboratory Practice in Tropical Countries. 2nd Edn., Cambridge University Press, Cambridge, UK., ISBN-13: 9781139449298.
[17] Das, K., and Murkherjee, A. K., (2007). “Crude petroleumoil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa isolated from a petroleum-oil contaminated soil from North-East India”. Bioresource Technology., 98 (2): 1339-1345.
[18] Dias, R., Ruberto, L., Hernandez, E., Vazquez, S., Balbo A., Panno, M., Cormack, W., (2012) ‘Bioremediation of an aged diesel oil-contaminated antartic soil evaluation of the ‘On site’ biostimulation strategy using different nutrient sources’ international biodeterioration and biodegradation Vol. 75 pp. 96-103.
[19] Dimitrow, D. N. and Markow, E. (2000). Behaviour of available forms of NPK in soils polluted by oil products. Poczwoznanie, Agrochimija Ekologia, 35 (3): 3-8.
[20] Ekpo M. A, and Nwaankpa, I. L. (2005). Effect of crude oil on microorganisms and growth of ginger (Zingiber officinale) in the tropics. J. Sustainable Trop. Agric. Res. 16: 67-71.
[21] Ekudanyo, E. O., and Obuekwe, O. O. (2004). “Effect of oil spill on soil physico-chemical properties of a spill site in a Typical Udipsamment of Niger Delta basin of Nigeria”. Environmental Monitoring and Assessment, Springer Netherlands, vol. 60, no. 2, pp. 235-249.
[22] Emufurieta, W. O., Kayode, A. A. & Coker, S. A. (1992). Mineralogy, geochemistry and economic evaluation of kaolin deposit near Ubulu – Uku, Awo- Omana and Buan in Southern Nigeria. Journal of Mineralogy and Geology, 28, 210 – 281.
[23] Encyclopædia Britannica. (2012). Encyclopædia Britannica Ultimate Reference Suite. Chicago: Encyclopædia Britannica.
[24] Essien, J. P., Udotong, I. R. (2008). Amino Acid Profile of Biodegraded Brewers Spent Grains (BSG). J. Appl. Sci. Environ. Manage. 12 (1): 109-111.
[25] Essien, J. P., Ebong, G. A., Asuquo, J. E., and Olajire, A. A (2012). Hydrocarbons contamination and microbial degradation in mangrove sediments of the Niger Delta region (Nigeria).
[26] Gallant Adrien TURF and Recreation (2004). Biostimulants: what they are and how they work.
[27] Ghazali, F. M, Rahman, RNZA, Salleh, A. B and Basri, M (2004). Biodegradation of hydrocarbons in soil by microbial consortium. Intl. Biodeter. Biodegrad. 54: 61-67.
[28] Hollday, C. H. and Deuel, L. E. (1994). Hydrocarbon and produced water contaminated soil and waste remediation, soil remediation for petroleum extraction industry, soil remediation seminar text. American petroleum institute publication, Washington D. C.
[29] Hou, D., and Al-Tabbaa A. A (2014) ‘Sustainability: A new imperative in contaminated land remediation’ Environmental Science and Policy Vol. 39 pp. 25-34.
[30] Institute of Petroleum. (IP) (2001). Standard Methods. The Institute of Petroleum, London, UK.
[31] Joo, H. S., Shoda, M. and Phae, C. G. (2007). Degradation of diesel oil in soil using a food waste composting process. Biodegradation 18 (5): 597–605.
[32] Kaczorek, I., Salek, K., Guzik, U., Teofil, J., and Cybulski, Z. (2013) ‘Biodegradation of alkyl derivatives of aromatic hydrocarbon and cell surface properties of a strain of pseudomonas stutzeri’ chemosphere Vol. 90(2) pp. 471-478.
[33] Kim, S. J., Choi, D. H., Sim, D. S. and Oh, Y. S. (2005) “Evaluation of bioremediation effectiveness on crude oil-contaminated sand,” Chemosphere 59(6): 845–852.
[34] Mbah, C. N., Nwite, J. N. and Nweke, I. A. (2009). Ameriolation of spent oil contaminated ultisol with organic wastes and its effect on soil properties and maize (Zea mays L) yield. World. J. Agric. Sci. 5(2), 163-168.
[35] Merckl, N., Schutze-Kraft, R and Arias, M. (2005). Influence of fertilizer level on phytoremediation of crude oil-contaminated soils with the tropical grass Brachiaria brizantha (Hochst. ex A. Rich.) Stapf. In: Phytoremediation of petroleum-contaminated soil. Merkl, N. (Ed), Margraf Publisher, Weikershim; pp 71-83.
[36] Miyashi, K., Sato, J. and Takahashi, N. (1996). Difference in the Effects of Dehusking during Formation of Seeds on Germination of Indica and Japaonia Rice (Oryza sativa L.). Annals of Botany, 71(8): 569-604.
[37] Njoku, K. L., Akinola, M. O. and Oboh, B. O. (2008). Germination, survival and growth of accession of Glycine max L. (Merril) (Soybean) and Lycopersicon esculentum L. (Tomato) in crude oil polluted soil. Res. J. Environ. Toxicol., 2(2): 77-84.
[38] Odedina, S. A., S. O. Odedina, S. Ayeni, A. A. Arowojolu, S. D. Areyeye and S. O. Ojeniyi, (2003). Effects of types of wood ash on soil fertility nutrient availability and yield of tomato and pepper. Niger. J. Soil Sci., 13: 61-67.
[39] Odjegba, V. and Sadiq, A. O. (2002). Effects of spent engine oil on the growth parameters, chlorophyll and protein levels of Amaranthus hybridus L. The Environmentalist, 22: 23-28.
[40] Okerentugba, P. O. and Ezeronye, O. U. (2003). Petroleum degrading potentials of single and mixed microbial cultures isolated from rivers and refinery effluents in Nigeria. Afr. J. Biotechnol. 2(9): 288-292.
[41] Okoh, I. A. (2006). Biodegradation Alternative in the Clean up of Petroleum Hydrocarbon Pollutants. Biotech. Mol. Biol. Rev. 1 (2): 38-50.
[42] Okon, J. E and Mbong, E. O. (2013). Effects of Nutrient Amendments of Spent Engine Oil Polluted Soil on Some Growth Parameters of Abelmoschus esculentus (L.) Moench. in South-South Nigeria. Bulletin of Environment, Pharmacology and Life Sciences Bull. Env. Pharmacol. Life Sci., Vol 2 (5) April 2013: 75-78 ©2013 Academy for Environment and Life Sciences, India Online ISSN 2277-1808.
[43] Okon, J. E., Esenowo, G. J. and Umoh, N. S. (2012). Effect of Crude Oil Pollution of Soil and Amelioration Treatment on the Growth of Eight Varieties of Manihot esculenta Crantz. International Journal of Chemical, Environmental and Pharmaceutical Research, 3 (2): 163-169.
[44] Oruru, J. A. (2014) ‘Is the use of brewery spent grain in bioremediation of diesel contaminated soil sustainable’ PhD. Dissertation University of Sunderland, UK.
[45] Raimi, M. O. (2008). The Effect of Vehicular Emission on Human Health, A Case Study of Yenagoa Motor Parks. Unpublished, A Seminar Paper Presented to the Department of Geography and Environmental Management, Niger Delta University, Wilberforce Island, Bayelsa State.
[46] Rainbow, A. and F. N. Wilson. (2002). Composting for Soil Improvement in the United Kingdom. In: Proceedings of the 12th International Soil Conservation Organization Conference. May 26-31, Beijing, China. Pp 63-67.
[47] Sabate, J., M. Vinas and A. M. Solanas, (2004). Laboratory scale bioremediation experiments on hydrocarbon contaminated soils. Intl. Biodeter. Biodegrad., 54: 19-25.
[48] Semple, K. T., Reid, B. J, and Fermor, T. R., (2001). “Impact of composting strategies on the treatment of soils contaminated with organic pollutants”. Environmental Pollution. 112 (1): 269-283.
[49] Senthilkumar, S., T. V. Viswanathan, A. D. Mercy, P. Gangadevi, K. Ally and K. Shyama (2010.) Chemical composition of brewery waste Tamilnadu J. Veterinary & Animal Sciences 6 (1) 49-51.
[50] Sihag, S. and Pathak, H. (2014) Factors Affecting the Rate of Biodegradation of Polyaromatic Hydrocarbons. International Journal of Pure Applied Bioscience [online]. 2 (3), pp. 185-202.
[51] Sun, W. H., Lo, J. B., Robert, F. M., Ray, C. and Tang, C. S. (2004). Phytoremediation of petroleum hydrocarbons in tropical coastal soil: I. Selection of promising woody plants. ESPR-Environ. Sci. Pollut. Res., 11 (4): 260-266.
[52] Tang, D., Yin, G., He, Y., Hu, S., Li, B., Li, L., Liang, H., and Borthakur, D. (2009). Recovery of protein from brewer’s spent grain by ultrafiltration. Biochem. Eng. J. 48: 1-5.
[53] Tian Y, Liu HJ, Zheng TL, Kwon KK, Kim SJ, Yan CL (2008) PAHs contamination and bacterial communities in mangrove surface sediments of the Jiulong River Estuary, China. Mar Pollut Bull 57: 707–715.
[54] Umoh, N. S. and Esenowo, G. J. (1996). Effects of used engine oil soil pollution on growth and yield of Walker, R. L., Burns, I. G. and Moorby, I. (2001). Response of plant growth rate to nitrogen supply: A comparison of relative addition and nitrogen interruption treatments. J. Expt. Bot., 52 (355): 309-317.
[55] Walter, M., Boyd-Wilson, K. S. H., McNaughton, D., Northcott, G., (2005). “Laboratory trials on the bioremediation of aged pentachlorophenol residues”. J. Int. Biodeter & Biodegr, 55 (3): 121-130.
[56] Yao, Z., Li, J., Xie, H., Conghai, Y., (2012) ‘Review on remediation technologies of soil contaminated by heavy metals’ procedia environmental sciences Vol. 16 pp. 722-729.
Author Information
  • Department of Community Medicine, Environmental Health Unit, Niger Delta University, Wilberforce Island, Nigeria

  • Department of Environmental Health, University of Uyo, Uyo, Nigeria

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    Raimi Morufu Olalekan, Sabinus Chibuzor Ezugwu. (2017). Influence of Organic Amendment on Microbial Activities and Growth of Pepper Cultured on Crude Oil Contaminated Niger Delta Soil. International Journal of Economy, Energy and Environment, 2(4), 56-76. https://doi.org/10.11648/j.ijeee.20170204.12

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    Raimi Morufu Olalekan; Sabinus Chibuzor Ezugwu. Influence of Organic Amendment on Microbial Activities and Growth of Pepper Cultured on Crude Oil Contaminated Niger Delta Soil. Int. J. Econ. Energy Environ. 2017, 2(4), 56-76. doi: 10.11648/j.ijeee.20170204.12

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    Raimi Morufu Olalekan, Sabinus Chibuzor Ezugwu. Influence of Organic Amendment on Microbial Activities and Growth of Pepper Cultured on Crude Oil Contaminated Niger Delta Soil. Int J Econ Energy Environ. 2017;2(4):56-76. doi: 10.11648/j.ijeee.20170204.12

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  • @article{10.11648/j.ijeee.20170204.12,
      author = {Raimi Morufu Olalekan and Sabinus Chibuzor Ezugwu},
      title = {Influence of Organic Amendment on Microbial Activities and Growth of Pepper Cultured on Crude Oil Contaminated Niger Delta Soil},
      journal = {International Journal of Economy, Energy and Environment},
      volume = {2},
      number = {4},
      pages = {56-76},
      doi = {10.11648/j.ijeee.20170204.12},
      url = {https://doi.org/10.11648/j.ijeee.20170204.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijeee.20170204.12},
      abstract = {Land contamination with crude oil is one of the challenges of effective crop production in the Niger Delta Region of Nigeria. This condition is inherent thus creates the need for developing effective remedial methods of managing contaminated lands as well as improve crop production. This study was designed to assess the effect of Brewers Spent Grains (BSG) on microbial activities and growth of Capsicum annum L cultured in crude oil contaminated soil. The results have shown that depending on the level of contamination, crude affected both the physical and chemical attributes of soil. It increased the acidity from pH 5.67 to a range of 5.58 - 5.64 and the total organic carbon content from 0.05% to 0.14% - 0.21 while the total nitrogen and available phosphorus levels of soil were remarkably reduced from 0.39% to 0.21% - 0.28%, and from 0.036% to 0.028% - 0.032% respectively. Amendment of contaminated soil with BSG had variable influence on the microbial properties of test soil. It increases the heterotrophic activity of bacteria in soil while retarding the activities of nitrogen fixing bacteria (Nitrosomonas sp and Nitrobacter sp). However at a stimulating amendment level of 150g of BSG in a 0.208% level of contamination, the activities of hydrocarbon degrading bacteria was enhanced from the hitherto low number of 3.6 log CFU/g found in test soil to 6.2 log CFU/g obtained on the 6th week of the remediation period. This resulted in 62.09% hydrocarbon degradation in soil at the end of the 8-week remediation course. BSG amendment of the oil contaminated had clear but variable influence on some agronomic traits of the cultured pepper. Growth was generally promoted by the amendment and the most significant (P<0.05) growth attributes affected were the plant height, number of branches, leaves, flowers and fruits as well as the leaf area. Pepper seedlings exposed to 0.624% of oil and remedied with 300g (1.5%) had the highest number of flowers but the flower did develop into fruits. However plant exposed to 0.416% of oil contamination and remedied with 0.75% of BSG produced flowers that formed fruits. Although the cultured pepper plants exhibited necrotic spots, this study has shown that plants grow in crude oil contaminated soil if alternative source of nutrients is provided. This tend to grow more in lightly contaminated soil and remediation using BSG has proven to enhance the activities of microorganisms and subsequent degradation of hydrocarbons in contaminated soil. It is highly recommended for use as soil conditioner to "unmask" nutrients in contaminated soils.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Influence of Organic Amendment on Microbial Activities and Growth of Pepper Cultured on Crude Oil Contaminated Niger Delta Soil
    AU  - Raimi Morufu Olalekan
    AU  - Sabinus Chibuzor Ezugwu
    Y1  - 2017/09/06
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ijeee.20170204.12
    DO  - 10.11648/j.ijeee.20170204.12
    T2  - International Journal of Economy, Energy and Environment
    JF  - International Journal of Economy, Energy and Environment
    JO  - International Journal of Economy, Energy and Environment
    SP  - 56
    EP  - 76
    PB  - Science Publishing Group
    SN  - 2575-5021
    UR  - https://doi.org/10.11648/j.ijeee.20170204.12
    AB  - Land contamination with crude oil is one of the challenges of effective crop production in the Niger Delta Region of Nigeria. This condition is inherent thus creates the need for developing effective remedial methods of managing contaminated lands as well as improve crop production. This study was designed to assess the effect of Brewers Spent Grains (BSG) on microbial activities and growth of Capsicum annum L cultured in crude oil contaminated soil. The results have shown that depending on the level of contamination, crude affected both the physical and chemical attributes of soil. It increased the acidity from pH 5.67 to a range of 5.58 - 5.64 and the total organic carbon content from 0.05% to 0.14% - 0.21 while the total nitrogen and available phosphorus levels of soil were remarkably reduced from 0.39% to 0.21% - 0.28%, and from 0.036% to 0.028% - 0.032% respectively. Amendment of contaminated soil with BSG had variable influence on the microbial properties of test soil. It increases the heterotrophic activity of bacteria in soil while retarding the activities of nitrogen fixing bacteria (Nitrosomonas sp and Nitrobacter sp). However at a stimulating amendment level of 150g of BSG in a 0.208% level of contamination, the activities of hydrocarbon degrading bacteria was enhanced from the hitherto low number of 3.6 log CFU/g found in test soil to 6.2 log CFU/g obtained on the 6th week of the remediation period. This resulted in 62.09% hydrocarbon degradation in soil at the end of the 8-week remediation course. BSG amendment of the oil contaminated had clear but variable influence on some agronomic traits of the cultured pepper. Growth was generally promoted by the amendment and the most significant (P<0.05) growth attributes affected were the plant height, number of branches, leaves, flowers and fruits as well as the leaf area. Pepper seedlings exposed to 0.624% of oil and remedied with 300g (1.5%) had the highest number of flowers but the flower did develop into fruits. However plant exposed to 0.416% of oil contamination and remedied with 0.75% of BSG produced flowers that formed fruits. Although the cultured pepper plants exhibited necrotic spots, this study has shown that plants grow in crude oil contaminated soil if alternative source of nutrients is provided. This tend to grow more in lightly contaminated soil and remediation using BSG has proven to enhance the activities of microorganisms and subsequent degradation of hydrocarbons in contaminated soil. It is highly recommended for use as soil conditioner to "unmask" nutrients in contaminated soils.
    VL  - 2
    IS  - 4
    ER  - 

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