American Journal of Plant Biology

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In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India

Received: 18 April 2018    Accepted: 03 May 2018    Published: 30 May 2018
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Abstract

The present investigation has been concentrated on the establishment of an efficacious method for in vitro regeneration of a Musa cultivar Gopi of Tripura. Focus has been greatly anchored to the sterilization procedure for assuring successful aseptic culture establishment of this cultivar. The culture protocol for shoot induction and proliferation comprised of four different treatments in basal MS medium fortified with 6-Benzylaminopurine of varying concentrations. The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l-1 for shoot bud induction and multiplication. Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l-1). Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid. The best rooting was manifested in treatment with IBA at concentration of 2mg l-1. The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India.

DOI 10.11648/j.ajpb.20180301.13
Published in American Journal of Plant Biology (Volume 3, Issue 1, March 2018)
Page(s) 12-16
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

In Vitro Regeneration, Musa Cultivar Gopi, Benzylaminopurine, Indole Butyric Acid, Clonal Propagation, Tripura

References
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[16] P. Karule, V. Dalvi, A. Kadu, R. Chaudhari, V. R. SubramaniamA. B. Patil “A commercial micropropagation protocol for virupakshi (AAB) banana via apical meristem” Afr J Biotechnol, vol.15(11), pp. 401-407. 2016.
[17] K. Maharani, S. Beura, P.S. Munsi “A Fast Protocol for in vitro cloning of Banana (Musaacuminata) cv. Amritpani”Int J Curr Microbiol, App Sci, vol. 6(10), pp. 586-594.2017.
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Author Information
  • Cytogenetics and Plant Biotechnology Lab., Department of Botany, Tripura University, Suryamaninagar, India

  • Cytogenetics and Plant Biotechnology Lab., Department of Botany, Tripura University, Suryamaninagar, India

  • Department of Botany, Utkal University, Bhubaneswar, India

  • Genetics and Molecular Biology, Council of Scientific and Industrial Research-National Botanical Research Institute, Lucknow, India

  • Cytogenetics and Plant Biotechnology Lab., Department of Botany, Tripura University, Suryamaninagar, India

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    Rabindra Kumar Sinha, Puja Rani Saha, Anath Bandhu Das, Satya Narayan Jena, Sangram Sinha. (2018). In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India. American Journal of Plant Biology, 3(1), 12-16. https://doi.org/10.11648/j.ajpb.20180301.13

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    ACS Style

    Rabindra Kumar Sinha; Puja Rani Saha; Anath Bandhu Das; Satya Narayan Jena; Sangram Sinha. In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India. Am. J. Plant Biol. 2018, 3(1), 12-16. doi: 10.11648/j.ajpb.20180301.13

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    AMA Style

    Rabindra Kumar Sinha, Puja Rani Saha, Anath Bandhu Das, Satya Narayan Jena, Sangram Sinha. In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India. Am J Plant Biol. 2018;3(1):12-16. doi: 10.11648/j.ajpb.20180301.13

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  • @article{10.11648/j.ajpb.20180301.13,
      author = {Rabindra Kumar Sinha and Puja Rani Saha and Anath Bandhu Das and Satya Narayan Jena and Sangram Sinha},
      title = {In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India},
      journal = {American Journal of Plant Biology},
      volume = {3},
      number = {1},
      pages = {12-16},
      doi = {10.11648/j.ajpb.20180301.13},
      url = {https://doi.org/10.11648/j.ajpb.20180301.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajpb.20180301.13},
      abstract = {The present investigation has been concentrated on the establishment of an efficacious method for in vitro regeneration of a Musa cultivar Gopi of Tripura. Focus has been greatly anchored to the sterilization procedure for assuring successful aseptic culture establishment of this cultivar. The culture protocol for shoot induction and proliferation comprised of four different treatments in basal MS medium fortified with 6-Benzylaminopurine of varying concentrations. The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l-1 for shoot bud induction and multiplication. Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l-1). Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid. The best rooting was manifested in treatment with IBA at concentration of 2mg l-1. The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India.},
     year = {2018}
    }
    

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    T1  - In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India
    AU  - Rabindra Kumar Sinha
    AU  - Puja Rani Saha
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    AU  - Satya Narayan Jena
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    DO  - 10.11648/j.ajpb.20180301.13
    T2  - American Journal of Plant Biology
    JF  - American Journal of Plant Biology
    JO  - American Journal of Plant Biology
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    SN  - 2578-8337
    UR  - https://doi.org/10.11648/j.ajpb.20180301.13
    AB  - The present investigation has been concentrated on the establishment of an efficacious method for in vitro regeneration of a Musa cultivar Gopi of Tripura. Focus has been greatly anchored to the sterilization procedure for assuring successful aseptic culture establishment of this cultivar. The culture protocol for shoot induction and proliferation comprised of four different treatments in basal MS medium fortified with 6-Benzylaminopurine of varying concentrations. The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l-1 for shoot bud induction and multiplication. Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l-1). Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid. The best rooting was manifested in treatment with IBA at concentration of 2mg l-1. The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India.
    VL  - 3
    IS  - 1
    ER  - 

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