To potentiate the immune responses of the Newcastle Disease vaccine, many adjuvants such as, Saponin, Paraffin oil, sunflower oil, Nigella Sativa oil, and Chitosan Nanoparticles “CHNPs”, were prepared with live and inactivated I-2 vaccines. The formulated vaccines were tested for their biological and physical characteristics, including sterility, safety, Immunogenicity protective efficacy, protein estimation for CHNPs, and the completion of the emulsification. The protein concentrations of pre and post encapsulation for CHNPs were 0.23g/100 ml and 0.065g/100 ml respectively. The encapsulation efficiency was 71.7%. To test the safety, immunogenicity and efficacy, a 180 white leghorn day-old-chicks divided randomly in to 9 groups of A, B, C, D, E, F, G, H, and I, a 20 chicks for each. Group A (Saponin + Iinactivated I-2), group B (Paraffin oil + Iinactivated I-2), group C (CH-NPs+ Inactivated I-2), group D (Saponin + Live + Inactivated), group E (Nigella Sativa oil+Live I-2), group F (Sunflower oil + Live I-2), group G (Saponin+Live I-2) each groups from A to G simultaneously and inrtaocularly (I/O) inoculated with 107 EID50 I-2 live vaccine. Group H (control group I) received only (Saponin+Inactivated), group I (control group II) received the placebo (Normal Saline) control group, in this group only 5 chicks out of 20 inoculated I/O with 106 EID50 of live I-2, and the remaining chicks were left unvaccinated. There was no evidence for bacterial growth for the tested and control groups, nor post vaccination reactions neither ND clinical signs observed (except for Nigella Sativa oil). Immunogenicity was estimated using ELISA test. The highest Abs mean titer was for group E While the lowest Abs mean titer was for group C. Mean values were analyzed using one way analysis of variance test (ANOVA). Mean Differences were considered to be statistically significant at P<.05. The nine comparisons were associated with statistically insignificant effect (P > .05). Groups A, B, C, D, and H challenged via mixing with clinically ill chickens for 14 days. The highest protection level was 90% for group D, while the lowest protection 50% was for group B (Paraffin oil+ Inactivated).
| Published in | Animal and Veterinary Sciences (Volume 9, Issue 3) |
| DOI | 10.11648/j.avs.20210903.13 |
| Page(s) | 56-64 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Adjuvant, Saponin, Mineral Oils, Vegetable Oils, Chitosan Nanoparticles, Newcastle Disease Vaccine, Immune Response, ELISA Test
| [1] | Linde AM, M Munir, S Zohari, K Stahl, C Baule, et al. (2011) Complete genome characterisation of a Newcastle disease virus isolated during an outbreak in Sweden in 1997. Virus Genes 41 (2): 165-173. |
| [2] | Narayanan MS, M Parthiban, P Sathiya, K Kumanan (2010) Molecular Detection of Newcastle disease virus using Flinders Molecular Detection of Newcastle Disease virus using Flinders Technology Associates-PCR Technology Associates-PCR. J Veterinarski Arhiv, 80 (1): 51-60. |
| [3] | Yune N, Abdela N (2017) Update on Epidemiology, Diagnosis and Control Technique of Newcastle Disease. Journal of Veterinary Science & Technology 8: 429. |
| [4] | Belloc C, Dupuis L, Deville S, Aucouturier J, Laval A. (2008) Evaluation of safety and immune response induced by several adjuvants included in Pasteurella multocida vaccines in chickens. Revue Méd. Vet.; 159 (7): 371-375. |
| [5] | Jang SI, Lillehoj HS, Lee SH, Lee KW, Lillehoj EP, Bertrand F, Dupuis L, Deville S (2011) enhances antibody and cell-mediated immune responses to profilin subunit antigen vaccination and promotes protection against. |
| [6] | Jang SI, Lillehoj HS, Lee SH, Lee KW, Park KW, Bauchan GR, Lillehoj EP, Bertrand F, Dupuis L, Deville S. (2010) Immunoenhancing effects of Montanide ISA oil-based adjuvants on recombinant coccidia antigen vaccination against Eimeria. |
| [7] | Jang SI, Lillehoj HS, Lee SH, Lee KW, Lillehoj EP, Hong YH, An DJ, Jeong W, Chun JE, Bertrand F, Dupuis L, Deville S, Ben Arous J. Vaccination with Clostridium perfringens recombinant proteins in combination with Montanide ISA 71 VG (2012) adjuvant increases protection against experimental necrotic enteritis in commercial broiler chickens. Vaccine.; 30: 5401-5406. |
| [8] | Yang, Y., Xing, R., Liu, S., Qin, Y., Li, K., Yu, H., & Li, P. (2020). Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine. Carbohydrate polymers, 229, 115423. https://doi.org/10.1016/j.carbpol.2019.115423 |
| [9] | Zhai RL, Xu HY, Wang YL, Qin ZM, Jiang SJ. [Study on immune efficacy of Newcastle disease chitosan microsphere vaccine]. Wei Sheng Wu Xue Bao. (2007) Aug; 47 (4): 692-6. Chinese. PMID: 17944374. |
| [10] | Ezeifeka G O, Nzewi K P, Amadi E S (2008) Effect of Oil Adjuvanted Newcastle Disease Vaccine on Immune Response in Chickens, Nigerian Journal of Microbiology, Vol. 22 (1): 1754-1758. |
| [11] | Rauw, F., Gardin, Y., Palya, V., Anbari, S., Gonze, M., Lemaire, S., van den Berg, T., & Lambrecht, B. (2010). The positive adjuvant effect of chitosan on antigen-specific cell-mediated immunity after chickens vaccination with live Newcastle disease vaccine. Veterinary immunology and immunopathology, 134 (3-4), 249–258. https://doi.org/10.1016/j.vetimm.2009.10.028 |
| [12] | Parimal Roy AT, Venugopalan A, Koteeswaran (1999) Efficacy of live adjuvanted mesogenic Newcastle disease vaccine in chickens: Vaccine 17 2674±2676 |
| [13] | Samina Y, Khinich B, Gutter A, Michael Peleg B A (1999) Day-old vaccination with live-in-oil vaccines: Newcastle disease (ND) and infectious bursal disease (IBD) in chicks and ND in turkey poults, Avian Pathology, 28: 1, 73-78. |
| [14] | Usman Waheed, Muhammad Siddique, Muhammad Arshad, Muhammad Ali, Ali Saeed (2013) Preparation of Newcastle Disease Vaccine from VG/GA Strain and its Evaluation in Commercial Broiler Chicks, Pakistan J. Zool., vol. 45 (2), pp. 339-344. |
| [15] | Zhao K, Chen G, Shi X-m, Gao T-t, Li W, et al.(2012) Preparation and Efficacy of a Live Newcastle Disease Virus Vaccine Encapsulated in Chitosan Nanoparticles. PLoS ONE 7 (12): e53314. doi: 10.1371/journal.pone.0053314. |
| [16] | Wisanu Wanasawaeng, Achara Tawatsin, Jiroj Sasipreeyajan, Prachak Poomvises, Niwat Chansiripornchai (2009) Development of Inactivated Newcastle Disease Vaccine using Palm Oil as an Adjuvant: Thai J. Vet. Med.,. 39 (1): 9-16. |
| [17] | Palya (1991) Manual for the production of marek's disease, Gumboro disease and inactivated Newcastle disease vaccines FAO animal production and health paper Rome, 1991 part B, preparation and control of inactivated Newcastle disease vaccine p. 29-43. |
| [18] | Jonase Salk M D, Angelam Laurent (1952) The use of adjuvants in studies on Influenza Immunization, From the eirus Research Laboratory, Department of Bacteriology, School of Medicine, University of Pittsburgh, Pittsburgh. |
| [19] | Office Intenationa de Epizootic (2012) Chapter 2.3.14. Newcastle disease in: Manual of Diagnostic tests and vaccines for Terrestrial Animals. |
| [20] | Murphy, Gibbs FA, Horzinek E P M C, Studdent, M S (1999) Paramyxo virus chapter In Veterinary virology (3rd Ed) Academic press, USA: 411-428. |
| [21] | Peleg BA, Samina I, Branner J.(1993) Immunization of chickens with live Newcastle disease vaccine adjuvanted with oil. Vaccine; 11: 1974±6. |
| [22] | Volkova, M. A., Irza, A. V., Chvala, I. A., Frolov, S. F., Drygin, V. V., & Kapczynski, D. R. (2014). Adjuvant effects of chitosan and calcium phosphate particles in an inactivated Newcastle disease vaccine. Avian diseases, 58 (1), 46–52. https://doi.org/10.1637/10510-020413-Reg.1 |
APA Style
Abdelmhmoud Atalmanan Abdelsadig, Sobhi Ahmed Mohammed Khair, Abdelgadir Ballal Mohamed, Tageldin Abdulla Mohamed Nour. (2021). Immunogenicity and Efficacy Study on Newcastle Disease Vaccine Using Many Adjuvants and Chitosan Nanoparticles. Animal and Veterinary Sciences, 9(3), 56-64. https://doi.org/10.11648/j.avs.20210903.13
ACS Style
Abdelmhmoud Atalmanan Abdelsadig; Sobhi Ahmed Mohammed Khair; Abdelgadir Ballal Mohamed; Tageldin Abdulla Mohamed Nour. Immunogenicity and Efficacy Study on Newcastle Disease Vaccine Using Many Adjuvants and Chitosan Nanoparticles. Anim. Vet. Sci. 2021, 9(3), 56-64. doi: 10.11648/j.avs.20210903.13
AMA Style
Abdelmhmoud Atalmanan Abdelsadig, Sobhi Ahmed Mohammed Khair, Abdelgadir Ballal Mohamed, Tageldin Abdulla Mohamed Nour. Immunogenicity and Efficacy Study on Newcastle Disease Vaccine Using Many Adjuvants and Chitosan Nanoparticles. Anim Vet Sci. 2021;9(3):56-64. doi: 10.11648/j.avs.20210903.13
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author = {Abdelmhmoud Atalmanan Abdelsadig and Sobhi Ahmed Mohammed Khair and Abdelgadir Ballal Mohamed and Tageldin Abdulla Mohamed Nour},
title = {Immunogenicity and Efficacy Study on Newcastle Disease Vaccine Using Many Adjuvants and Chitosan Nanoparticles},
journal = {Animal and Veterinary Sciences},
volume = {9},
number = {3},
pages = {56-64},
doi = {10.11648/j.avs.20210903.13},
url = {https://doi.org/10.11648/j.avs.20210903.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20210903.13},
abstract = {To potentiate the immune responses of the Newcastle Disease vaccine, many adjuvants such as, Saponin, Paraffin oil, sunflower oil, Nigella Sativa oil, and Chitosan Nanoparticles “CHNPs”, were prepared with live and inactivated I-2 vaccines. The formulated vaccines were tested for their biological and physical characteristics, including sterility, safety, Immunogenicity protective efficacy, protein estimation for CHNPs, and the completion of the emulsification. The protein concentrations of pre and post encapsulation for CHNPs were 0.23g/100 ml and 0.065g/100 ml respectively. The encapsulation efficiency was 71.7%. To test the safety, immunogenicity and efficacy, a 180 white leghorn day-old-chicks divided randomly in to 9 groups of A, B, C, D, E, F, G, H, and I, a 20 chicks for each. Group A (Saponin + Iinactivated I-2), group B (Paraffin oil + Iinactivated I-2), group C (CH-NPs+ Inactivated I-2), group D (Saponin + Live + Inactivated), group E (Nigella Sativa oil+Live I-2), group F (Sunflower oil + Live I-2), group G (Saponin+Live I-2) each groups from A to G simultaneously and inrtaocularly (I/O) inoculated with 107 EID50 I-2 live vaccine. Group H (control group I) received only (Saponin+Inactivated), group I (control group II) received the placebo (Normal Saline) control group, in this group only 5 chicks out of 20 inoculated I/O with 106 EID50 of live I-2, and the remaining chicks were left unvaccinated. There was no evidence for bacterial growth for the tested and control groups, nor post vaccination reactions neither ND clinical signs observed (except for Nigella Sativa oil). Immunogenicity was estimated using ELISA test. The highest Abs mean titer was for group E While the lowest Abs mean titer was for group C. Mean values were analyzed using one way analysis of variance test (ANOVA). Mean Differences were considered to be statistically significant at PP > .05). Groups A, B, C, D, and H challenged via mixing with clinically ill chickens for 14 days. The highest protection level was 90% for group D, while the lowest protection 50% was for group B (Paraffin oil+ Inactivated).},
year = {2021}
}
TY - JOUR T1 - Immunogenicity and Efficacy Study on Newcastle Disease Vaccine Using Many Adjuvants and Chitosan Nanoparticles AU - Abdelmhmoud Atalmanan Abdelsadig AU - Sobhi Ahmed Mohammed Khair AU - Abdelgadir Ballal Mohamed AU - Tageldin Abdulla Mohamed Nour Y1 - 2021/06/07 PY - 2021 N1 - https://doi.org/10.11648/j.avs.20210903.13 DO - 10.11648/j.avs.20210903.13 T2 - Animal and Veterinary Sciences JF - Animal and Veterinary Sciences JO - Animal and Veterinary Sciences SP - 56 EP - 64 PB - Science Publishing Group SN - 2328-5850 UR - https://doi.org/10.11648/j.avs.20210903.13 AB - To potentiate the immune responses of the Newcastle Disease vaccine, many adjuvants such as, Saponin, Paraffin oil, sunflower oil, Nigella Sativa oil, and Chitosan Nanoparticles “CHNPs”, were prepared with live and inactivated I-2 vaccines. The formulated vaccines were tested for their biological and physical characteristics, including sterility, safety, Immunogenicity protective efficacy, protein estimation for CHNPs, and the completion of the emulsification. The protein concentrations of pre and post encapsulation for CHNPs were 0.23g/100 ml and 0.065g/100 ml respectively. The encapsulation efficiency was 71.7%. To test the safety, immunogenicity and efficacy, a 180 white leghorn day-old-chicks divided randomly in to 9 groups of A, B, C, D, E, F, G, H, and I, a 20 chicks for each. Group A (Saponin + Iinactivated I-2), group B (Paraffin oil + Iinactivated I-2), group C (CH-NPs+ Inactivated I-2), group D (Saponin + Live + Inactivated), group E (Nigella Sativa oil+Live I-2), group F (Sunflower oil + Live I-2), group G (Saponin+Live I-2) each groups from A to G simultaneously and inrtaocularly (I/O) inoculated with 107 EID50 I-2 live vaccine. Group H (control group I) received only (Saponin+Inactivated), group I (control group II) received the placebo (Normal Saline) control group, in this group only 5 chicks out of 20 inoculated I/O with 106 EID50 of live I-2, and the remaining chicks were left unvaccinated. There was no evidence for bacterial growth for the tested and control groups, nor post vaccination reactions neither ND clinical signs observed (except for Nigella Sativa oil). Immunogenicity was estimated using ELISA test. The highest Abs mean titer was for group E While the lowest Abs mean titer was for group C. Mean values were analyzed using one way analysis of variance test (ANOVA). Mean Differences were considered to be statistically significant at PP > .05). Groups A, B, C, D, and H challenged via mixing with clinically ill chickens for 14 days. The highest protection level was 90% for group D, while the lowest protection 50% was for group B (Paraffin oil+ Inactivated). VL - 9 IS - 3 ER -
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