As a potent antifungal drug, fluconazole clinically used to eradicate both systemic and superficial mycoses resulting in hepatotoxicity. The objective of the current study was to evaluate hepatotoxicity and genotoxicity in newborn male mice. Mice were treated orally with 0.5 ml fluconazole doses of (0, 25, 50, and 100 mg/kgbw) per day for five consecutive weeks. Micronucleus test, chromosomal aberrations in bone marrow cells, histopathological investigation and DNA fragmentation in the liver tissue was done. Micronuclei are significantly noticed in bone marrow cells of mice given 50 and 100 mg/kgbw fluconazole however, there is no effect on the genotoxicity induced by 25 mg/kgbw dose of fluconazole. A dose-dependent and significant increase in structural and numerical chromosomal aberrations were detected in the 50 and 100 mg/kgbw fluconazole-treated group but a 100 mg/kg was highly significant. The chromosomal aberrations were manifested in hypoploidy, deletion, centric fusion and stickiness. Besides, hepatocellular massive infiltration, cytoplasmic vacuolation, congestion and dilatation in the central veins were seen in 50 and 100 mg/kgbw fluconazole. Interestingly, 25mg/kgbw fluconazole-treated mice showed mild hepatocellular degeneration. Consequently, these findings confirmed that fluconazole to a greater extent was a potent hepatotoxic drug in vivo in newborn mice.
| Published in | Biomedical Sciences (Volume 7, Issue 1) |
| DOI | 10.11648/j.bs.20210701.11 |
| Page(s) | 1-9 |
| Creative Commons |
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Copyright © The Author(s), 2024. Published by Science Publishing Group |
Fluconazole, Genotoxicity, Micronucleus, Chromosomal Aberration, Hepatotoxicity
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APA Style
Azza Attia, Cecil Matta, Reda Elmazoudy, Zeinab Elhenshery. (2021). Cytogenetic and Ultrastructural Studies of Effects of Antifungal Drug, Fluconazole on Liver of New-born Mice. Biomedical Sciences, 7(1), 1-9. https://doi.org/10.11648/j.bs.20210701.11
ACS Style
Azza Attia; Cecil Matta; Reda Elmazoudy; Zeinab Elhenshery. Cytogenetic and Ultrastructural Studies of Effects of Antifungal Drug, Fluconazole on Liver of New-born Mice. Biomed. Sci. 2021, 7(1), 1-9. doi: 10.11648/j.bs.20210701.11
AMA Style
Azza Attia, Cecil Matta, Reda Elmazoudy, Zeinab Elhenshery. Cytogenetic and Ultrastructural Studies of Effects of Antifungal Drug, Fluconazole on Liver of New-born Mice. Biomed Sci. 2021;7(1):1-9. doi: 10.11648/j.bs.20210701.11
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Download@article{10.11648/j.bs.20210701.11,
author = {Azza Attia and Cecil Matta and Reda Elmazoudy and Zeinab Elhenshery},
title = {Cytogenetic and Ultrastructural Studies of Effects of Antifungal Drug, Fluconazole on Liver of New-born Mice},
journal = {Biomedical Sciences},
volume = {7},
number = {1},
pages = {1-9},
doi = {10.11648/j.bs.20210701.11},
url = {https://doi.org/10.11648/j.bs.20210701.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20210701.11},
abstract = {As a potent antifungal drug, fluconazole clinically used to eradicate both systemic and superficial mycoses resulting in hepatotoxicity. The objective of the current study was to evaluate hepatotoxicity and genotoxicity in newborn male mice. Mice were treated orally with 0.5 ml fluconazole doses of (0, 25, 50, and 100 mg/kgbw) per day for five consecutive weeks. Micronucleus test, chromosomal aberrations in bone marrow cells, histopathological investigation and DNA fragmentation in the liver tissue was done. Micronuclei are significantly noticed in bone marrow cells of mice given 50 and 100 mg/kgbw fluconazole however, there is no effect on the genotoxicity induced by 25 mg/kgbw dose of fluconazole. A dose-dependent and significant increase in structural and numerical chromosomal aberrations were detected in the 50 and 100 mg/kgbw fluconazole-treated group but a 100 mg/kg was highly significant. The chromosomal aberrations were manifested in hypoploidy, deletion, centric fusion and stickiness. Besides, hepatocellular massive infiltration, cytoplasmic vacuolation, congestion and dilatation in the central veins were seen in 50 and 100 mg/kgbw fluconazole. Interestingly, 25mg/kgbw fluconazole-treated mice showed mild hepatocellular degeneration. Consequently, these findings confirmed that fluconazole to a greater extent was a potent hepatotoxic drug in vivo in newborn mice.},
year = {2021}
}
TY - JOUR T1 - Cytogenetic and Ultrastructural Studies of Effects of Antifungal Drug, Fluconazole on Liver of New-born Mice AU - Azza Attia AU - Cecil Matta AU - Reda Elmazoudy AU - Zeinab Elhenshery Y1 - 2021/01/12 PY - 2021 N1 - https://doi.org/10.11648/j.bs.20210701.11 DO - 10.11648/j.bs.20210701.11 T2 - Biomedical Sciences JF - Biomedical Sciences JO - Biomedical Sciences SP - 1 EP - 9 PB - Science Publishing Group SN - 2575-3932 UR - https://doi.org/10.11648/j.bs.20210701.11 AB - As a potent antifungal drug, fluconazole clinically used to eradicate both systemic and superficial mycoses resulting in hepatotoxicity. The objective of the current study was to evaluate hepatotoxicity and genotoxicity in newborn male mice. Mice were treated orally with 0.5 ml fluconazole doses of (0, 25, 50, and 100 mg/kgbw) per day for five consecutive weeks. Micronucleus test, chromosomal aberrations in bone marrow cells, histopathological investigation and DNA fragmentation in the liver tissue was done. Micronuclei are significantly noticed in bone marrow cells of mice given 50 and 100 mg/kgbw fluconazole however, there is no effect on the genotoxicity induced by 25 mg/kgbw dose of fluconazole. A dose-dependent and significant increase in structural and numerical chromosomal aberrations were detected in the 50 and 100 mg/kgbw fluconazole-treated group but a 100 mg/kg was highly significant. The chromosomal aberrations were manifested in hypoploidy, deletion, centric fusion and stickiness. Besides, hepatocellular massive infiltration, cytoplasmic vacuolation, congestion and dilatation in the central veins were seen in 50 and 100 mg/kgbw fluconazole. Interestingly, 25mg/kgbw fluconazole-treated mice showed mild hepatocellular degeneration. Consequently, these findings confirmed that fluconazole to a greater extent was a potent hepatotoxic drug in vivo in newborn mice. VL - 7 IS - 1 ER -
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