Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage

Ke Niu; Fengjie Xu; Guangyong Wang; Manhong Zeng

doi:doi:10.11648/j.bs.20210703.11

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Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage

Ke Niu, Fengjie Xu, Guangyong Wang, Manhong Zeng

Published in Biomedical Sciences (Volume 7, Issue 3)

Received: 2 June 2021 Accepted: 19 June 2021 Published: 15 July 2021

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Abstract

Neonatal hypoxic-ischemic brain damage (HIBD) is the main cause of severe neurological diseases and death in newborns. Macamide B is an effective monomer extracted from Maca (Lepidium meyenii Walpers), which has important biological activities such as neuroprotection and neuromodulation. The purpose of this study was to investigate whether Macamide B can exert neuroprotective effects on HIBD in newborn mice by regulating Silent information regulator factor 2-related enzyme 1 (SIRT1). A modified Rice-Vannucci method was used to construct the HIBD model of newborn mice. The pups were divided into the following groups: sham group, HI group, and Macamide B group. On the first and third days after hypoxic-ischemic (HI), immunofluorescence and Western blot experiments were used to detect the expression level of SIRT1 in the brain tissue of infants. The results of the immunofluorescence experiment showed that compared with the sham group, the expression level of SIRT1 is significantly decreased in HI group pups, while the expression level of SIRT1 in pups pretreated with Macamide B increased significantly. The results of Western blot experiments are consistent with the results of immunofluorescence experiments. Our data indicate that Macamide B may exert a neuroprotective effect on HIBD in newborn mice by up-regulating the expression of SIRT1. Macamide B may become a new medicine effective in preventing and treating HIBD.

Published in	Biomedical Sciences (Volume 7, Issue 3)
DOI	10.11648/j.bs.20210703.11
Page(s)	64-68
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Macamide B, SIRT1, Neonatal Hypoxic-ischemic Brain Damage

References

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APA Style

Ke Niu, Fengjie Xu, Guangyong Wang, Manhong Zeng. (2021). Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage. Biomedical Sciences, 7(3), 64-68. https://doi.org/10.11648/j.bs.20210703.11

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ACS Style

Ke Niu; Fengjie Xu; Guangyong Wang; Manhong Zeng. Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage. Biomed. Sci. 2021, 7(3), 64-68. doi: 10.11648/j.bs.20210703.11

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AMA Style

Ke Niu, Fengjie Xu, Guangyong Wang, Manhong Zeng. Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage. Biomed Sci. 2021;7(3):64-68. doi: 10.11648/j.bs.20210703.11

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@article{10.11648/j.bs.20210703.11,
  author = {Ke Niu and Fengjie Xu and Guangyong Wang and Manhong Zeng},
  title = {Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage},
  journal = {Biomedical Sciences},
  volume = {7},
  number = {3},
  pages = {64-68},
  doi = {10.11648/j.bs.20210703.11},
  url = {https://doi.org/10.11648/j.bs.20210703.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20210703.11},
  abstract = {Neonatal hypoxic-ischemic brain damage (HIBD) is the main cause of severe neurological diseases and death in newborns. Macamide B is an effective monomer extracted from Maca (Lepidium meyenii Walpers), which has important biological activities such as neuroprotection and neuromodulation. The purpose of this study was to investigate whether Macamide B can exert neuroprotective effects on HIBD in newborn mice by regulating Silent information regulator factor 2-related enzyme 1 (SIRT1). A modified Rice-Vannucci method was used to construct the HIBD model of newborn mice. The pups were divided into the following groups: sham group, HI group, and Macamide B group. On the first and third days after hypoxic-ischemic (HI), immunofluorescence and Western blot experiments were used to detect the expression level of SIRT1 in the brain tissue of infants. The results of the immunofluorescence experiment showed that compared with the sham group, the expression level of SIRT1 is significantly decreased in HI group pups, while the expression level of SIRT1 in pups pretreated with Macamide B increased significantly. The results of Western blot experiments are consistent with the results of immunofluorescence experiments. Our data indicate that Macamide B may exert a neuroprotective effect on HIBD in newborn mice by up-regulating the expression of SIRT1. Macamide B may become a new medicine effective in preventing and treating HIBD.},
 year = {2021}
}

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TY - JOUR
T1 - Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage
AU - Ke Niu
AU - Fengjie Xu
AU - Guangyong Wang
AU - Manhong Zeng
Y1 - 2021/07/15
PY - 2021
N1 - https://doi.org/10.11648/j.bs.20210703.11
DO - 10.11648/j.bs.20210703.11
T2 - Biomedical Sciences
JF - Biomedical Sciences
JO - Biomedical Sciences
SP - 64
EP - 68
PB - Science Publishing Group
SN - 2575-3932
UR - https://doi.org/10.11648/j.bs.20210703.11
AB - Neonatal hypoxic-ischemic brain damage (HIBD) is the main cause of severe neurological diseases and death in newborns. Macamide B is an effective monomer extracted from Maca (Lepidium meyenii Walpers), which has important biological activities such as neuroprotection and neuromodulation. The purpose of this study was to investigate whether Macamide B can exert neuroprotective effects on HIBD in newborn mice by regulating Silent information regulator factor 2-related enzyme 1 (SIRT1). A modified Rice-Vannucci method was used to construct the HIBD model of newborn mice. The pups were divided into the following groups: sham group, HI group, and Macamide B group. On the first and third days after hypoxic-ischemic (HI), immunofluorescence and Western blot experiments were used to detect the expression level of SIRT1 in the brain tissue of infants. The results of the immunofluorescence experiment showed that compared with the sham group, the expression level of SIRT1 is significantly decreased in HI group pups, while the expression level of SIRT1 in pups pretreated with Macamide B increased significantly. The results of Western blot experiments are consistent with the results of immunofluorescence experiments. Our data indicate that Macamide B may exert a neuroprotective effect on HIBD in newborn mice by up-regulating the expression of SIRT1. Macamide B may become a new medicine effective in preventing and treating HIBD.
VL - 7
IS - 3
ER -

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Author Information

Ke Niu

School of Clinical Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Fengjie Xu

School of Clinical Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Guangyong Wang

Department of Forensic Science, Intermediate People’s Court of Huangshan City, Huangshan, People's Republic of China
Manhong Zeng

School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China

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APA Style

Ke Niu, Fengjie Xu, Guangyong Wang, Manhong Zeng. (2021). Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage. Biomedical Sciences, 7(3), 64-68. https://doi.org/10.11648/j.bs.20210703.11

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ACS Style

Ke Niu; Fengjie Xu; Guangyong Wang; Manhong Zeng. Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage. Biomed. Sci. 2021, 7(3), 64-68. doi: 10.11648/j.bs.20210703.11

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AMA Style

Ke Niu, Fengjie Xu, Guangyong Wang, Manhong Zeng. Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage. Biomed Sci. 2021;7(3):64-68. doi: 10.11648/j.bs.20210703.11

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@article{10.11648/j.bs.20210703.11,
  author = {Ke Niu and Fengjie Xu and Guangyong Wang and Manhong Zeng},
  title = {Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage},
  journal = {Biomedical Sciences},
  volume = {7},
  number = {3},
  pages = {64-68},
  doi = {10.11648/j.bs.20210703.11},
  url = {https://doi.org/10.11648/j.bs.20210703.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20210703.11},
  abstract = {Neonatal hypoxic-ischemic brain damage (HIBD) is the main cause of severe neurological diseases and death in newborns. Macamide B is an effective monomer extracted from Maca (Lepidium meyenii Walpers), which has important biological activities such as neuroprotection and neuromodulation. The purpose of this study was to investigate whether Macamide B can exert neuroprotective effects on HIBD in newborn mice by regulating Silent information regulator factor 2-related enzyme 1 (SIRT1). A modified Rice-Vannucci method was used to construct the HIBD model of newborn mice. The pups were divided into the following groups: sham group, HI group, and Macamide B group. On the first and third days after hypoxic-ischemic (HI), immunofluorescence and Western blot experiments were used to detect the expression level of SIRT1 in the brain tissue of infants. The results of the immunofluorescence experiment showed that compared with the sham group, the expression level of SIRT1 is significantly decreased in HI group pups, while the expression level of SIRT1 in pups pretreated with Macamide B increased significantly. The results of Western blot experiments are consistent with the results of immunofluorescence experiments. Our data indicate that Macamide B may exert a neuroprotective effect on HIBD in newborn mice by up-regulating the expression of SIRT1. Macamide B may become a new medicine effective in preventing and treating HIBD.},
 year = {2021}
}

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