Hepatitis B Virus A1762T/G1764A Mutation Has a Minor Effect on the Viral Spliced DNA Expression

Zongxin Li; Yihan Xiao; Yuanhai Zhou; Ting Tang; Pin Yang; Long Sun; Xiuji Cui

doi:doi:10.11648/j.bs.20230901.15

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Hepatitis B Virus A1762T/G1764A Mutation Has a Minor Effect on the Viral Spliced DNA Expression

Zongxin Li, Yihan Xiao, Yuanhai Zhou, Ting Tang, Pin Yang, Long Sun, Xiuji Cui

Published in Biomedical Sciences (Volume 9, Issue 1)

Received: 6 March 2023 Accepted: 24 March 2023 Published: 31 March 2023

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Abstract

Background: Hepatitis B virus (HBV) is the major cause of chronic hepatitis B, which can lead to liver cirrhosis and hepatocellular carcinoma. During HBV replication, splicing of viral RNA frequently occurs, and the spliced RNA or DNA has been reported to be related to the development of liver disease. HBV transcription is mainly regulated by core promoter ranging from nucleotide 1613 to 1849 and the A1762T/G1764A mutation, which can increase the viral transcription, is frequently detected in this region. Objective: The study aimed to analyze the effect of the A1762T/G1764A mutation in the core promoter of HBV on viral RNA splicing. Methods: The wild type (WT) and the A1762T/G1764A mutant HBV genome were cloned into the pCDNA3.1 vector, which was then transiently transfected into HepG2 cells. The intracellular HBV DNA and core protein were determined by the Southern blot and the Western blot, respectively. The relative level of HBSD was examined by quantitative PCR using TB green reagent. Results: the A1762T/G1764A mutation could enhance the ability of viral replication, however, had a minor effect on HBSD expression. Conclusion: The double mutation in CP could regulate the viral transcription, but was not involved in the viral RNA splicing.

Published in	Biomedical Sciences (Volume 9, Issue 1)
DOI	10.11648/j.bs.20230901.15
Page(s)	30-34
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Hepatitis B Virus, Basal Core Promoter, Viral Replication

References

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APA Style

Zongxin Li, Yihan Xiao, Yuanhai Zhou, Ting Tang, Pin Yang, et al. (2023). Hepatitis B Virus A1762T/G1764A Mutation Has a Minor Effect on the Viral Spliced DNA Expression. Biomedical Sciences, 9(1), 30-34. https://doi.org/10.11648/j.bs.20230901.15

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ACS Style

Zongxin Li; Yihan Xiao; Yuanhai Zhou; Ting Tang; Pin Yang, et al. Hepatitis B Virus A1762T/G1764A Mutation Has a Minor Effect on the Viral Spliced DNA Expression. Biomed. Sci. 2023, 9(1), 30-34. doi: 10.11648/j.bs.20230901.15

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AMA Style

Zongxin Li, Yihan Xiao, Yuanhai Zhou, Ting Tang, Pin Yang, et al. Hepatitis B Virus A1762T/G1764A Mutation Has a Minor Effect on the Viral Spliced DNA Expression. Biomed Sci. 2023;9(1):30-34. doi: 10.11648/j.bs.20230901.15

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@article{10.11648/j.bs.20230901.15,
  author = {Zongxin Li and Yihan Xiao and Yuanhai Zhou and Ting Tang and Pin Yang and Long Sun and Xiuji Cui},
  title = {Hepatitis B Virus A1762T/G1764A Mutation Has a Minor Effect on the Viral Spliced DNA Expression},
  journal = {Biomedical Sciences},
  volume = {9},
  number = {1},
  pages = {30-34},
  doi = {10.11648/j.bs.20230901.15},
  url = {https://doi.org/10.11648/j.bs.20230901.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20230901.15},
  abstract = {Background: Hepatitis B virus (HBV) is the major cause of chronic hepatitis B, which can lead to liver cirrhosis and hepatocellular carcinoma. During HBV replication, splicing of viral RNA frequently occurs, and the spliced RNA or DNA has been reported to be related to the development of liver disease. HBV transcription is mainly regulated by core promoter ranging from nucleotide 1613 to 1849 and the A1762T/G1764A mutation, which can increase the viral transcription, is frequently detected in this region. Objective: The study aimed to analyze the effect of the A1762T/G1764A mutation in the core promoter of HBV on viral RNA splicing. Methods: The wild type (WT) and the A1762T/G1764A mutant HBV genome were cloned into the pCDNA3.1 vector, which was then transiently transfected into HepG2 cells. The intracellular HBV DNA and core protein were determined by the Southern blot and the Western blot, respectively. The relative level of HBSD was examined by quantitative PCR using TB green reagent. Results: the A1762T/G1764A mutation could enhance the ability of viral replication, however, had a minor effect on HBSD expression. Conclusion: The double mutation in CP could regulate the viral transcription, but was not involved in the viral RNA splicing.},
 year = {2023}
}

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TY  - JOUR
T1  - Hepatitis B Virus A1762T/G1764A Mutation Has a Minor Effect on the Viral Spliced DNA Expression
AU  - Zongxin Li
AU  - Yihan Xiao
AU  - Yuanhai Zhou
AU  - Ting Tang
AU  - Pin Yang
AU  - Long Sun
AU  - Xiuji Cui
Y1  - 2023/03/31
PY  - 2023
N1  - https://doi.org/10.11648/j.bs.20230901.15
DO  - 10.11648/j.bs.20230901.15
T2  - Biomedical Sciences
JF  - Biomedical Sciences
JO  - Biomedical Sciences
SP  - 30
EP  - 34
PB  - Science Publishing Group
SN  - 2575-3932
UR  - https://doi.org/10.11648/j.bs.20230901.15
AB  - Background: Hepatitis B virus (HBV) is the major cause of chronic hepatitis B, which can lead to liver cirrhosis and hepatocellular carcinoma. During HBV replication, splicing of viral RNA frequently occurs, and the spliced RNA or DNA has been reported to be related to the development of liver disease. HBV transcription is mainly regulated by core promoter ranging from nucleotide 1613 to 1849 and the A1762T/G1764A mutation, which can increase the viral transcription, is frequently detected in this region. Objective: The study aimed to analyze the effect of the A1762T/G1764A mutation in the core promoter of HBV on viral RNA splicing. Methods: The wild type (WT) and the A1762T/G1764A mutant HBV genome were cloned into the pCDNA3.1 vector, which was then transiently transfected into HepG2 cells. The intracellular HBV DNA and core protein were determined by the Southern blot and the Western blot, respectively. The relative level of HBSD was examined by quantitative PCR using TB green reagent. Results: the A1762T/G1764A mutation could enhance the ability of viral replication, however, had a minor effect on HBSD expression. Conclusion: The double mutation in CP could regulate the viral transcription, but was not involved in the viral RNA splicing.
VL  - 9
IS  - 1
ER  -

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Author Information

Zongxin Li

Department of Pathogenic Biology, School of Basic Medicine and Life Science, Hainan Medical University, Haikou, China
Yihan Xiao

Department of Clinical Laboratory, The Second Affiliated Hospital of Hainan Medical University, Haikou, China
Yuanhai Zhou

Department of Pathogenic Biology, School of Basic Medicine and Life Science, Hainan Medical University, Haikou, China
Ting Tang

Department of Pathogenic Biology, School of Basic Medicine and Life Science, Hainan Medical University, Haikou, China
Pin Yang

Department of Pathogenic Biology, School of Basic Medicine and Life Science, Hainan Medical University, Haikou, China
Long Sun

Department of Infectious Disease, The First Affiliated Hospital of Hainan Medical University, Haikou, China
Xiuji Cui

Department of Pathogenic Biology, School of Basic Medicine and Life Science, Hainan Medical University, Haikou, China

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APA Style

Zongxin Li, Yihan Xiao, Yuanhai Zhou, Ting Tang, Pin Yang, et al. (2023). Hepatitis B Virus A1762T/G1764A Mutation Has a Minor Effect on the Viral Spliced DNA Expression. Biomedical Sciences, 9(1), 30-34. https://doi.org/10.11648/j.bs.20230901.15

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ACS Style

Zongxin Li; Yihan Xiao; Yuanhai Zhou; Ting Tang; Pin Yang, et al. Hepatitis B Virus A1762T/G1764A Mutation Has a Minor Effect on the Viral Spliced DNA Expression. Biomed. Sci. 2023, 9(1), 30-34. doi: 10.11648/j.bs.20230901.15

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AMA Style

Zongxin Li, Yihan Xiao, Yuanhai Zhou, Ting Tang, Pin Yang, et al. Hepatitis B Virus A1762T/G1764A Mutation Has a Minor Effect on the Viral Spliced DNA Expression. Biomed Sci. 2023;9(1):30-34. doi: 10.11648/j.bs.20230901.15

Copy | Download

@article{10.11648/j.bs.20230901.15,
  author = {Zongxin Li and Yihan Xiao and Yuanhai Zhou and Ting Tang and Pin Yang and Long Sun and Xiuji Cui},
  title = {Hepatitis B Virus A1762T/G1764A Mutation Has a Minor Effect on the Viral Spliced DNA Expression},
  journal = {Biomedical Sciences},
  volume = {9},
  number = {1},
  pages = {30-34},
  doi = {10.11648/j.bs.20230901.15},
  url = {https://doi.org/10.11648/j.bs.20230901.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20230901.15},
  abstract = {Background: Hepatitis B virus (HBV) is the major cause of chronic hepatitis B, which can lead to liver cirrhosis and hepatocellular carcinoma. During HBV replication, splicing of viral RNA frequently occurs, and the spliced RNA or DNA has been reported to be related to the development of liver disease. HBV transcription is mainly regulated by core promoter ranging from nucleotide 1613 to 1849 and the A1762T/G1764A mutation, which can increase the viral transcription, is frequently detected in this region. Objective: The study aimed to analyze the effect of the A1762T/G1764A mutation in the core promoter of HBV on viral RNA splicing. Methods: The wild type (WT) and the A1762T/G1764A mutant HBV genome were cloned into the pCDNA3.1 vector, which was then transiently transfected into HepG2 cells. The intracellular HBV DNA and core protein were determined by the Southern blot and the Western blot, respectively. The relative level of HBSD was examined by quantitative PCR using TB green reagent. Results: the A1762T/G1764A mutation could enhance the ability of viral replication, however, had a minor effect on HBSD expression. Conclusion: The double mutation in CP could regulate the viral transcription, but was not involved in the viral RNA splicing.},
 year = {2023}
}

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TY  - JOUR
T1  - Hepatitis B Virus A1762T/G1764A Mutation Has a Minor Effect on the Viral Spliced DNA Expression
AU  - Zongxin Li
AU  - Yihan Xiao
AU  - Yuanhai Zhou
AU  - Ting Tang
AU  - Pin Yang
AU  - Long Sun
AU  - Xiuji Cui
Y1  - 2023/03/31
PY  - 2023
N1  - https://doi.org/10.11648/j.bs.20230901.15
DO  - 10.11648/j.bs.20230901.15
T2  - Biomedical Sciences
JF  - Biomedical Sciences
JO  - Biomedical Sciences
SP  - 30
EP  - 34
PB  - Science Publishing Group
SN  - 2575-3932
UR  - https://doi.org/10.11648/j.bs.20230901.15
AB  - Background: Hepatitis B virus (HBV) is the major cause of chronic hepatitis B, which can lead to liver cirrhosis and hepatocellular carcinoma. During HBV replication, splicing of viral RNA frequently occurs, and the spliced RNA or DNA has been reported to be related to the development of liver disease. HBV transcription is mainly regulated by core promoter ranging from nucleotide 1613 to 1849 and the A1762T/G1764A mutation, which can increase the viral transcription, is frequently detected in this region. Objective: The study aimed to analyze the effect of the A1762T/G1764A mutation in the core promoter of HBV on viral RNA splicing. Methods: The wild type (WT) and the A1762T/G1764A mutant HBV genome were cloned into the pCDNA3.1 vector, which was then transiently transfected into HepG2 cells. The intracellular HBV DNA and core protein were determined by the Southern blot and the Western blot, respectively. The relative level of HBSD was examined by quantitative PCR using TB green reagent. Results: the A1762T/G1764A mutation could enhance the ability of viral replication, however, had a minor effect on HBSD expression. Conclusion: The double mutation in CP could regulate the viral transcription, but was not involved in the viral RNA splicing.
VL  - 9
IS  - 1
ER  -

Copy | Download