Culture based diversity of Rhizobacteria associated with Sorghum wild relatives were identified by this study. Rhizobacteria, which include rhizosphere bacteria, rhizoplane bacteria and endophytic bacteria were isolated and identified. The isolates were also evaluated and screened for their ability to solubilize phosphates on Pikovskaya’s agar plates. One hundred twenty-six rhizobacteria were isolated on nutrient agar from the samples. Out of these Rhizobacterial isolates, 21 of them were endophyte bacteria, 40 of them were rhizosphere bacteria and 65 of them were rhizoplane bacteria. The 126 isolated rhizobacteria were clustered into 36 similar representative Morphotype groups. And, they were identified using GEN III Biolog microbial identification system. Gram negative rhizobacteria were more predominant than Gram positive rhizobacteria. Genus Pseudomonas was found as the most dominant Rhizobacterial taxa among the identified P-solubilizing Rhizobacteria and Non-P-solubilizing Rhizobacteria isolates. Forty seven percent (47.2%) of the clustered rhizobacterial isolates showed clearly visible haloes (>0.50 cm) around their colonies on Pikovskaya’s agar after seven days of incubation at 30 ± 2°C. They showed a significant difference of solubilization index (SI) [p<0.05] and ranged from 1.3 to 5.3 with a mean value of 2.4. Gram positive bacteria (Bacillus cibi) produced the largest solubilisation index when compared with the Gram-negative isolates. This study indicated that P-solubilizing rhizobacteria associated with Sorghum Wild Relative have very large phosphate solubilization index. Thus, it is recommended to include them in research programs that intend to screen P-solubilizing bacteria for microbial inoculant development.
| Published in | Frontiers in Environmental Microbiology (Volume 7, Issue 2) |
| DOI | 10.11648/j.fem.20210702.13 |
| Page(s) | 63-68 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Rhizobacterial Diversity, Sorghum Wild Relatives, P-solubilizing Rhizobacteria, Biolog
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APA Style
Eleni Belay, Befekadu Teshome. (2021). Diversity of Rhizobacteria Associated with Sorghum Wild Relatives in Assosa Zone of Ethiopia and Their Ability to Solubilize Phosphate. Frontiers in Environmental Microbiology, 7(2), 63-68. https://doi.org/10.11648/j.fem.20210702.13
ACS Style
Eleni Belay; Befekadu Teshome. Diversity of Rhizobacteria Associated with Sorghum Wild Relatives in Assosa Zone of Ethiopia and Their Ability to Solubilize Phosphate. Front. Environ. Microbiol. 2021, 7(2), 63-68. doi: 10.11648/j.fem.20210702.13
AMA Style
Eleni Belay, Befekadu Teshome. Diversity of Rhizobacteria Associated with Sorghum Wild Relatives in Assosa Zone of Ethiopia and Their Ability to Solubilize Phosphate. Front Environ Microbiol. 2021;7(2):63-68. doi: 10.11648/j.fem.20210702.13
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Download@article{10.11648/j.fem.20210702.13,
author = {Eleni Belay and Befekadu Teshome},
title = {Diversity of Rhizobacteria Associated with Sorghum Wild Relatives in Assosa Zone of Ethiopia and Their Ability to Solubilize Phosphate},
journal = {Frontiers in Environmental Microbiology},
volume = {7},
number = {2},
pages = {63-68},
doi = {10.11648/j.fem.20210702.13},
url = {https://doi.org/10.11648/j.fem.20210702.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20210702.13},
abstract = {Culture based diversity of Rhizobacteria associated with Sorghum wild relatives were identified by this study. Rhizobacteria, which include rhizosphere bacteria, rhizoplane bacteria and endophytic bacteria were isolated and identified. The isolates were also evaluated and screened for their ability to solubilize phosphates on Pikovskaya’s agar plates. One hundred twenty-six rhizobacteria were isolated on nutrient agar from the samples. Out of these Rhizobacterial isolates, 21 of them were endophyte bacteria, 40 of them were rhizosphere bacteria and 65 of them were rhizoplane bacteria. The 126 isolated rhizobacteria were clustered into 36 similar representative Morphotype groups. And, they were identified using GEN III Biolog microbial identification system. Gram negative rhizobacteria were more predominant than Gram positive rhizobacteria. Genus Pseudomonas was found as the most dominant Rhizobacterial taxa among the identified P-solubilizing Rhizobacteria and Non-P-solubilizing Rhizobacteria isolates. Forty seven percent (47.2%) of the clustered rhizobacterial isolates showed clearly visible haloes (>0.50 cm) around their colonies on Pikovskaya’s agar after seven days of incubation at 30 ± 2°C. They showed a significant difference of solubilization index (SI) [p<0.05] and ranged from 1.3 to 5.3 with a mean value of 2.4. Gram positive bacteria (Bacillus cibi) produced the largest solubilisation index when compared with the Gram-negative isolates. This study indicated that P-solubilizing rhizobacteria associated with Sorghum Wild Relative have very large phosphate solubilization index. Thus, it is recommended to include them in research programs that intend to screen P-solubilizing bacteria for microbial inoculant development.},
year = {2021}
}
TY - JOUR T1 - Diversity of Rhizobacteria Associated with Sorghum Wild Relatives in Assosa Zone of Ethiopia and Their Ability to Solubilize Phosphate AU - Eleni Belay AU - Befekadu Teshome Y1 - 2021/05/21 PY - 2021 N1 - https://doi.org/10.11648/j.fem.20210702.13 DO - 10.11648/j.fem.20210702.13 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 63 EP - 68 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20210702.13 AB - Culture based diversity of Rhizobacteria associated with Sorghum wild relatives were identified by this study. Rhizobacteria, which include rhizosphere bacteria, rhizoplane bacteria and endophytic bacteria were isolated and identified. The isolates were also evaluated and screened for their ability to solubilize phosphates on Pikovskaya’s agar plates. One hundred twenty-six rhizobacteria were isolated on nutrient agar from the samples. Out of these Rhizobacterial isolates, 21 of them were endophyte bacteria, 40 of them were rhizosphere bacteria and 65 of them were rhizoplane bacteria. The 126 isolated rhizobacteria were clustered into 36 similar representative Morphotype groups. And, they were identified using GEN III Biolog microbial identification system. Gram negative rhizobacteria were more predominant than Gram positive rhizobacteria. Genus Pseudomonas was found as the most dominant Rhizobacterial taxa among the identified P-solubilizing Rhizobacteria and Non-P-solubilizing Rhizobacteria isolates. Forty seven percent (47.2%) of the clustered rhizobacterial isolates showed clearly visible haloes (>0.50 cm) around their colonies on Pikovskaya’s agar after seven days of incubation at 30 ± 2°C. They showed a significant difference of solubilization index (SI) [p<0.05] and ranged from 1.3 to 5.3 with a mean value of 2.4. Gram positive bacteria (Bacillus cibi) produced the largest solubilisation index when compared with the Gram-negative isolates. This study indicated that P-solubilizing rhizobacteria associated with Sorghum Wild Relative have very large phosphate solubilization index. Thus, it is recommended to include them in research programs that intend to screen P-solubilizing bacteria for microbial inoculant development. VL - 7 IS - 2 ER -
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