The bacteria Pseudomonas is being used as biological control agent and it is safe alternative for the fungicide. Our objective was to optimize the nutrition and environmental condition for biomass and antifungal efficiency and to evaluate it against wilt disease caused by F. Oxysporum in soybean. Pseudomonas fluorescence, showed antagonistic properties, in vitro, against the pathogen Fusarium oxysporum by dual culture. Effect of the separated secondary metabolites on the fungal growth by broth dilution technique and antifungal activity by agar well diffusion technique was studied. The present studies, purified metabolite of the Pseudomonas fluorescence inhibited the Fusarium oxysporum at the concentration of 0.5% that was analyzed by well diffusion method. The secondary metabolites was subjected for TLC. Further, purified effective metabolite was analyzed by HPLC and GC-MS with its Retension time molecular weight as 2,4 DAPG. In our studies we optimized, the metabolite concentration by OVAT. The number of variable factors was optimized showed that Glucose, Peptone and mineral salts are significantly increasing the production of DAPG up to 38.47ug/ml. Furthermore, RSM suggested that glucose peptone and mineral salt would result in the maximum production of the 2,4Diacetyl Phloroglucinol 46.5 ug/ml. Under natural condition, P. fluorescence formulation was effective in reducing Fusarium wilt in soybean. Seed treatment with Pseudomonas protected crop from disease in comparing of fungicide. In addition, the obtained results showed that bacterial treatment signifi-cantly increased the growth parameters as well as dry weights and yield.
| Published in | Frontiers in Environmental Microbiology (Volume 8, Issue 2) |
| DOI | 10.11648/j.fem.20220802.13 |
| Page(s) | 35-45 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
2,4-Diacetylphloroglucinol, Biocontrol, Metabolite, Pseudomonas, Fusarium
| [1] | Anonymous. (2018). The Soybean Processors Association of India (SOPA) report http://www.sopa.org/india-oilseeds-areaproduction-and-productivity |
| [2] | Amrate, P. K., Pancheshwar, D. K., and Shrivastava M. K. (2020). Screening of Genotypes to Identify the Resistance Source against Major Diseases of Soybean under High Disease Pressure Conditions International Journal of Current Microbiology and Applied Sciences 9 (5): 1739-1745. |
| [3] | Dhingra, O. D., and J. B. Sinclair (1978) Biology and pathology of Macrophomina phaseolina. Imprensa Universitaria, Universidade Feederal de Vicosa, Minas Gerais, BR. |
| [4] | S. S. Gote, P. H. Ghante, U. A. Asalkar, V. S. Mete and S. K. Deshmukh (2021) In-vitro Efficacy of Bio-agents against Sudden Death Syndrome (wilt) Disease of Soybean Caused by Fusarium oxysporum f. sp. virguliformeInt. J. Curr. Microbiol. App. Sci 10 (02): 2808-2812. |
| [5] | Sinclair, J. B. and Blackman, P. A. (1989). Compendium of soybean diseases. The American Phytopathological Society Str., J-106. |
| [6] | Abdel monaim MK., elyousr abo KAM, Morsy M (2011). Effectiveness of plant extracts on suppression of damping-off and wilt diseases of lupine (Lupinus termis Forsik), 30 (20): 185-191. |
| [7] | Singh, Simranjeet, Singh, Nasib, Kumar, Vijay, Datta, Shivika Wani, Abdul, Singh, Damnita Singh, Karan, Singh, Joginder PY (2016) Toxicity, monitoring and biodegradation of the fungicide carbendazim, Environmental Chemistry Letters 14 (3). |
| [8] | Copeland L. O., & Mcdonald, M. B. (2005) Principles of Seed Science and Technology. Fifth Edition. Kluwer Academic Publishers, Massachusetts. |
| [9] | Andrés JA, Correa NS, Rosas SB (1998) Survival and symbiotic properties of Bradyrhizobium japonicum in the presence of thiram. Isolation of fungicide resistant strains. Biol Fertil Soils 26: 141–145. |
| [10] | Campo, R. J. and Hungria, M. (2000). Inoculação da soja em sistema plantio direto. In: Anais do 1° Simpósio sobre Fertilidade do Solo e Nutrição de Plantas no Sistema Plantio Direto. Associação dos Engenheiros Agrônomos dos Campos Gerais, Ponta Grossa, Brazil, pp 146-160. |
| [11] | Kloepper JW, Ryu CM, and Zhang S induced Systemic Resistance and Promotion of Plant Growth by Bacillus sppPhytopathology 94 (11): 1259-66. |
| [12] | Budge SP, Whipps JM. 2001. Potential for integrated control of Sclerotinia sclerotiorum in glasshouse lettuce using Coniothyrium minitans and reduced fungicide application. Phytopathology 91221–227. |
| [13] | Haas, D. and De´fago, G. (2005) Biological control of soilborne pathogens by fluorescent pseudomonads. Nat Rev Microbiol 3, 307–319. |
| [14] | Sharma A, Diwevidi VD, Smita Singh, Pawar KK, JermanM, SinghLB, Singh S, Srivastaw D (2017) International Journal of Biotechnology and Bioengineering Research. Volume 4 (3): 175-180. |
| [15] | Duffy, B. K., and Défago, G. 1999. Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains. Appl. Environ. Microbiol. 65: 2429-2438. |
| [16] | Shanahan, P., O’Sullivan, D. J., Simpson, P., Glennon, J. D., and O’Gara, F. 1992. Isolation of 2,4-diacethylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production. Appl. Environ. Microbiol. 58: 353-358. |
| [17] | Bajpai A., Singh B., Joshi S., Johri BN., (2017) Production and Characterization of an Antifungal Compoundfrom Pseudomonas protegens Strain W45, Proceedings of national academy of sciences 88 (3). |
| [18] | S. R. Prabhukarthikeyan and T. Raguchander (2016) Antifungal Metabolites of Pseudomonas fluorescens against Pythium aphanidermatum JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 10 (1): 579-584. |
| [19] | Bonsall, R. F., Weller, D. M., and Thomashow, L. S. (1997). Quantification of 2,4-diacetylphloroglucinol produced by fluorescent Pseudomonas spp. in vitro and in the rhizosphere of wheat. Appl. Environ. Microbiol. 63, 951–955. |
| [20] | Shanmugaiah, V., Ramesh, S., Jayaprakashvel, M., Mathivanan, N., 2006. Biocontrol and plant growth promoting potential of Pseudomonas sp. MML2212 from the rice rhizosphere. Mitteilungen-biologischen bundesanstalt fur land und forestwirstschaft. pp. 408: 320. |
| [21] | Couillerot, C0. Prigent-Combaret, J. Caballero-Mellado, Y. Moënne-Loccoz (2009) Pseudomonas fluorescens and closely-related fluorescent pseudomonads as biocontrol agents of soil-borne phytopathogens, 48 (5): 505-512. |
| [22] | Mohammadi P, Tozlu E, Kotan R, Kotan MS. 2020. Potential of some bacteria for biological control of postharvest citrus green mold caused by Penicillum digitatum. Plant Prot Sci. 53: 1–14. |
| [23] | C. Voisard, C. Keel, D. Hass and G. Defago, “Cyanide Production by Pseudomonas fluorescence Suppress Helps Black Root Rot of Tobacco under Gnotobiotic Condi-tions,” EMBO Journal, Vol. 8, No. 2, 1989, 351-358. |
| [24] | Fenton AM, Stephens PM, Crowley J, O’Callaghan M, O’Gara F (1992) Exploiting genes involved in 2,4-diacetylphloroglucinol biosynthesis to improve the biocontrol ability of a pseudomonad strain. Appl Environ Microbiol 58, 3872-3878. |
| [25] | Keel, C., Schnider, U., Maurhefor, M., Voisard, C., Laville, K., Burger, U., Wirthner, P., Hass, D. and Defago, G. (1992). Suppression of rootrot diseases by Pseudomonas fluorescens CHAO: Importance of the bacterial secondary metabolite, 2, 4-diacetylphloroglucinol. Mol. Plant Microbe Interact. 5 (1): 4. |
| [26] | Kraus J, Loper JE. Characterization of a Genomic Region Required for Production of the Antibiotic Pyoluteorin by the Biological Control Agent Pseudomonas fluorescens Pf-5. Appl Environ Microbiol. 1995 Mar; 61 (3): 849–854. |
| [27] | W. F. Pfender, V. P. Fieland, L. M. Ganio, R. J. Seidler, Microbial community structure and activity in wheat straw after inoculation with biological control organisms, Applied Soil Ecology, 1996 3 (1): 69-78. |
| [28] | Ayyadurai, N., P. R. Naik, M. S. Rao, R. S. Kumar, S. K. Samrat, M. Manohar and N. Sakthivel. 2005. Isolation and characterization of a novel banana rhizosphere as fungal antagonist and microbial adjuvant in micropropagation of banana. J. Appl. Microbiol. 100: 926-937. |
| [29] | Q Yang, X Y Wang, Y X Yao, J R Gould, L M CaoA new species of Sclerodermus (Hymenoptera: Bethylidae) parasitizing Agrilus planipennis (Coleoptera: Buprestidae) from China, with a key to Chinese species in the genusAnnals of the Entomological. |
| [30] | Battu. Prasanna Reddy, M. S. Reddy, and K. Vijay Krishna Kumar (2009). Characterization of antifungal metabolites of Pseudomonas fluorescens and their effect on mycelial growth of Magnaporthegrisea and RhizoctoniasolaniInternational Journal of PharmTech Research 1 (4) pp: 1490-1493. |
| [31] | Notz, R., Maurhofer, M., Dubach, H., Haas, D., and Defago, G. (2002). Fusaric acid-producing strains of Fusarium oxysporum alter 2,4-diacetylphloroglucinol biosynthetic gene expression in Pseudomonas fluorescens CHA0 in vitro and in the rhizosphere of wheat. Appl. Environ. Microbiol. 68, 2229–2235. doi: 10.1128/AEM.68.5.2229-2235.2002. |
| [32] | Sumant Chaubey, Malini Kotak and Archana G (2015) New Method for Isolation of Plant Probiotic Fluorescent Pseudomonad and Characterization for 2,4-Diacetylphluoroglucinol Production under Different Carbon Sources and Phosphate Levels J. Plant Pathol Microb 6 (2): 1-7. |
| [33] | Raaijmakers, J. M., Vlami, M., and de Souza, J. T. 2002. Antibiotic production by bacterial biocontrol agents. Antonie van Leeuwenhoek 81: 537-547. |
| [34] | Achkar j., Xian M., Zhao H., Frost J. W (2004). Biosynthesis of Phloroglucinol Journal of Americal Chemical Society 127 (15) 5332-5333. |
| [35] | Shanahan P, O’Sullivan DJ, Simpson P, Glennon JD, O’Gara F. Isolation of 2,4-diacetylphloroglucinol from a pseudomonad and investigation of physiological parameters influencing its production. Appl Environ Microbiol 1992; 58: 353–8. |
| [36] | Jorge T. de souza (2004) showed DAPG from Pseudomonas flouroscence P60 32ug/ml of purified metabolite is completely inhibiting Phythiurg/10.1094/MPMI.1998.11.9.847. |
| [37] | Liu T., Kang J., Liu, (2021). Thymol as a critical component of Thymus vulgaris L. essential oil combats Pseudomonas aeruginosa by intercalating DNA and inactivating biofilm, LWT, 136 (2) 110354. |
| [38] | Kumar A., Saini S, Wray V, Nimtz M. Prakash A. (2012) Characterization of an antifungal compound produced by Bacillus sp. strain A (5) F that inhibits Sclerotinia sclerotiorum. J. Basic Microbiology 52: 670-678. |
| [39] | Haggag WM., Soud MAE ((2012) Production and Optimization of Pseudomonas fluorescens Biomass and Metabolites for Biocontrol of Strawberry Grey Mould American Journal of Plant Sciences, 2012, 3, 836-845. |
| [40] | Yousef, A. E. and C. Carlstrom, 2003. Practical Basic Technique. Food Microbiology. A Laboratory Manual, P: 277. Wiley-Interscience. A John Wiley and Sons, Inc. Publication. |
| [41] | Rodriguez, F., and Pfender, W. F. (1997) Antibiosis and antagonism of Sclerotinia homoeocarpa and Drechslera poae by Pseudomonas fluorescens Pf-5 in vitro and in planta. Phytopathology 87: 614–621. 10.1094/PHYTO.1997.87.6.614. |
| [42] | Saharan K, Sarma MVRK, Srivastava R, Sharma AK, Johri BN, Prakash A, et al. Development of non-sterile inorganic carrier-based formulations of fluorescent pseudomonad R62 and R81 and evaluation of their efficacy on agricultural crops. Appl Soil Ecol 2010, doi: 10.1016/j.apsoil.2010.08.004. |
| [43] | Sarma, M. V. R. K., Sahai, V. and Bisaria, V. S. (2020) Genetic algorithm based medium optimization for enhanced production of fluorescent pseudomonad R81 and siderophores. Biochem Eng J 47, 100–108. |
| [44] | Hultberg M, Alsanius B. Influence of nitrogen source on 2,4- diacetylphloroglucinol production by the biocontrol strain Pf-5. Open Microbiol J 2008; 2: 74–8. |
APA Style
Kiran Paliwal, Anil Prakash. (2022). Optimization & Molecular Characterization of an Antifungal Metabolite (2,4-diacetylphloroglucinol) from Pseudomonas fluorescence in Management of Fusarium wilt in soybean. Frontiers in Environmental Microbiology, 8(2), 35-45. https://doi.org/10.11648/j.fem.20220802.13
ACS Style
Kiran Paliwal; Anil Prakash. Optimization & Molecular Characterization of an Antifungal Metabolite (2,4-diacetylphloroglucinol) from Pseudomonas fluorescence in Management of Fusarium wilt in soybean. Front. Environ. Microbiol. 2022, 8(2), 35-45. doi: 10.11648/j.fem.20220802.13
AMA Style
Kiran Paliwal, Anil Prakash. Optimization & Molecular Characterization of an Antifungal Metabolite (2,4-diacetylphloroglucinol) from Pseudomonas fluorescence in Management of Fusarium wilt in soybean. Front Environ Microbiol. 2022;8(2):35-45. doi: 10.11648/j.fem.20220802.13
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Download@article{10.11648/j.fem.20220802.13,
author = {Kiran Paliwal and Anil Prakash},
title = {Optimization & Molecular Characterization of an Antifungal Metabolite (2,4-diacetylphloroglucinol) from Pseudomonas fluorescence in Management of Fusarium wilt in soybean},
journal = {Frontiers in Environmental Microbiology},
volume = {8},
number = {2},
pages = {35-45},
doi = {10.11648/j.fem.20220802.13},
url = {https://doi.org/10.11648/j.fem.20220802.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20220802.13},
abstract = {The bacteria Pseudomonas is being used as biological control agent and it is safe alternative for the fungicide. Our objective was to optimize the nutrition and environmental condition for biomass and antifungal efficiency and to evaluate it against wilt disease caused by F. Oxysporum in soybean. Pseudomonas fluorescence, showed antagonistic properties, in vitro, against the pathogen Fusarium oxysporum by dual culture. Effect of the separated secondary metabolites on the fungal growth by broth dilution technique and antifungal activity by agar well diffusion technique was studied. The present studies, purified metabolite of the Pseudomonas fluorescence inhibited the Fusarium oxysporum at the concentration of 0.5% that was analyzed by well diffusion method. The secondary metabolites was subjected for TLC. Further, purified effective metabolite was analyzed by HPLC and GC-MS with its Retension time molecular weight as 2,4 DAPG. In our studies we optimized, the metabolite concentration by OVAT. The number of variable factors was optimized showed that Glucose, Peptone and mineral salts are significantly increasing the production of DAPG up to 38.47ug/ml. Furthermore, RSM suggested that glucose peptone and mineral salt would result in the maximum production of the 2,4Diacetyl Phloroglucinol 46.5 ug/ml. Under natural condition, P. fluorescence formulation was effective in reducing Fusarium wilt in soybean. Seed treatment with Pseudomonas protected crop from disease in comparing of fungicide. In addition, the obtained results showed that bacterial treatment signifi-cantly increased the growth parameters as well as dry weights and yield.},
year = {2022}
}
TY - JOUR T1 - Optimization & Molecular Characterization of an Antifungal Metabolite (2,4-diacetylphloroglucinol) from Pseudomonas fluorescence in Management of Fusarium wilt in soybean AU - Kiran Paliwal AU - Anil Prakash Y1 - 2022/06/21 PY - 2022 N1 - https://doi.org/10.11648/j.fem.20220802.13 DO - 10.11648/j.fem.20220802.13 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 35 EP - 45 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20220802.13 AB - The bacteria Pseudomonas is being used as biological control agent and it is safe alternative for the fungicide. Our objective was to optimize the nutrition and environmental condition for biomass and antifungal efficiency and to evaluate it against wilt disease caused by F. Oxysporum in soybean. Pseudomonas fluorescence, showed antagonistic properties, in vitro, against the pathogen Fusarium oxysporum by dual culture. Effect of the separated secondary metabolites on the fungal growth by broth dilution technique and antifungal activity by agar well diffusion technique was studied. The present studies, purified metabolite of the Pseudomonas fluorescence inhibited the Fusarium oxysporum at the concentration of 0.5% that was analyzed by well diffusion method. The secondary metabolites was subjected for TLC. Further, purified effective metabolite was analyzed by HPLC and GC-MS with its Retension time molecular weight as 2,4 DAPG. In our studies we optimized, the metabolite concentration by OVAT. The number of variable factors was optimized showed that Glucose, Peptone and mineral salts are significantly increasing the production of DAPG up to 38.47ug/ml. Furthermore, RSM suggested that glucose peptone and mineral salt would result in the maximum production of the 2,4Diacetyl Phloroglucinol 46.5 ug/ml. Under natural condition, P. fluorescence formulation was effective in reducing Fusarium wilt in soybean. Seed treatment with Pseudomonas protected crop from disease in comparing of fungicide. In addition, the obtained results showed that bacterial treatment signifi-cantly increased the growth parameters as well as dry weights and yield. VL - 8 IS - 2 ER -
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