Corn silk contain inherently substantial flavonoid that contribute to its antioxidant and anticancer activity. The objective of the present study was to fabricate a system for efficient delivery of the anticancer compounds using microencapsulation technique. Methanolic corn silk extract was microencapsulated in the polymer Poly (d,l-lactide-co-glycolide) – PLGA using the solvent extraction method. The physicochemical properties such as size, morphology and physical state of free and encapsulated microparticles were measured by dynamic light scattering, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in vitro release of compounds were studied and quantified using High Performance Liquid Chromatography (HPLC). Spherical and relatively small (d=485.9) polymeric microparticles were obtained containing flavonoids with encapsulation efficiency (EE) of 60.66%. In vitro release profile exhibit a slow, sustained release and follows the first order kinetic with release rate 3.34 x 10-3 m-1s-1. The release characteristics data showed that the drug is released from the microsphere even after 108 h. For in vitro cell-based assays, the MTT cell viability assay was performed on HeLa, NIH 3T3 cell lines while cellular uptake of the drug was studied using fluorescence microscopy. Fluorescence studies confirm drug uptake by the cells within 24 h of treatment. For confirmation of mode of cell death Flow Cytometry and DNA ladder assay was performed. The blank polymeric microparticles were non-toxic to cell while, the drug loaded microparticles exhibit apoptic cell death. Thus an efficient delivery system is achieved after encapsulation, that provides protection and controlled release of the bioactive compounds.
| Published in | Journal of Drug Design and Medicinal Chemistry (Volume 7, Issue 1) |
| DOI | 10.11648/j.jddmc.20210701.13 |
| Page(s) | 12-22 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Anticancer, Flavonoids, PLGA, Microencapsulation, Drug Release, Apoptosis
| [1] | F. H. Igney and P. H. Krammer, “Death and anti-death: tumour resistance to apoptosis.,” Nat. Rev. Cancer, vol. 2, no. 4, pp. 277–288, Apr. 2002, doi: 10.1038/nrc776. |
| [2] | E. Safarzadeh, S. S. Shotorbani, and B. Baradaran, “Herbal Medicine as Inducers of Apoptosis in Cancer Treatment,” vol. 4, no. Suppl 1, pp. 421–427, 2014, doi: 10.5681/apb.2014.062. |
| [3] | O. Prakash, A. Kumar, and P. Kumar, “Anticancer Potential of Plants and Natural Products: A Review,” no. April 2017, 2013, doi: 10.12691/ajps-1-6-1. |
| [4] | Y. S. Jhanwar, C. Divgi, N. York, and N. York, “Current Status of Therapy of Solid Tumors,” pp. 141–151. |
| [5] | V. Guillemard and H. U. Saragovi, “Novel approaches for targeted cancer therapy.,” Curr. Cancer Drug Targets, vol. 4, no. 4, pp. 313–326, Jun. 2004, doi: 10.2174/1568009043332989. |
| [6] | S. Antonia, J. J. Mulé, and J. S. Weber, “Current developments of immunotherapy in the clinic,” Curr. Opin. Immunol., vol. 16, no. 2, p. 130—136, Apr. 2004, doi: 10.1016/j.coi.2004.01.012. |
| [7] | F. Caponigro, M. Basile, V. de Rosa, and N. Normanno, “New drugs in cancer therapy, National Tumor Institute, Naples, 17-18 June 2004.,” Anti-cancer drugs, vol. 16, no. 2. England, pp. 211–221, Feb. 2005, doi: 10.1097/00001813-200502000-00014. |
| [8] | T. J. Miner, “Palliative Surgery for Advanced Cancer: Lessons Learned in Patient Selection and Outcome Assessment,” Am. J. Clin. Oncol., vol. 28, no. 4, 2005, [Online]. Available: https://journals.lww.com/amjclinicaloncology/Fulltext/2005/08000/Palliative_Surgery_for_Advanced_Cancer__Lessons.15.aspx. |
| [9] | M. Elshaikh, M. Ljungman, R. Ten Haken, and A. S. Lichter, “Advances in Radiation Oncology,” Annu. Rev. Med., vol. 57, no. 1, pp. 19–31, Jan. 2006, doi: 10.1146/annurev.med.57.121304.131431. |
| [10] | J. J. Smith, P. Tully, and R. M. Padberg, “Chemoprevention: A Primary Cancer Prevention Strategy,” Semin. Oncol. Nurs., vol. 21, no. 4, pp. 243–251, 2005, doi: https://doi.org/10.1016/j.soncn.2005.06.009. |
| [11] | M. B. Sporn and K. T. Liby, “Cancer chemoprevention: scientific promise, clinical uncertainty.,” Nat. Clin. Pract. Oncol., vol. 2, no. 10, pp. 518–525, Oct. 2005, doi: 10.1038/ncponc0319. |
| [12] | D. E. Brenner and A. J. Gescher, “Cancer chemoprevention: lessons learned and future directions,” Br. J. Cancer, vol. 93, no. 7, pp. 735–739, Oct. 2005, doi: 10.1038/sj.bjc.6602765. |
| [13] | E. S. Kim and W. K. Hong, “An Apple a Day…Does It Really Keep the Doctor Away? The Current State of Cancer Chemoprevention,” JNCI J. Natl. Cancer Inst., vol. 97, no. 7, pp. 468–470, Apr. 2005, doi: 10.1093/jnci/dji103. |
| [14] | J. R. Patil, K. N. Chidambara Murthy, G. K. Jayaprakasha, M. B. Chetti, and B. S. Patil, “Bioactive compounds from Mexican lime (Citrus aurantifolia) juice induce apoptosis in human pancreatic cells.,” J. Agric. Food Chem., vol. 57, no. 22, pp. 10933–10942, Nov. 2009, doi: 10.1021/jf901718u. |
| [15] | Q. Hu and Z. Deng, “Protective effects of flavonoids from corn silk on oxidative stress induced by exhaustive exercise in mice,” vol. 10, no. 16, pp. 3163–3167, 2011, doi: 10.5897/AJB10.2671. |
| [16] | Grieve M., A Modern Herbal. New York; Dover Publication, 1971. |
| [17] | Z. Maksimović, D. Malencić, and N. Kovacević, “Polyphenol contents and antioxidant activity of Maydis stigma extracts.,” Bioresour. Technol., vol. 96, no. 8, pp. 873–877, May 2005, doi: 10.1016/j.biortech.2004.09.006. |
| [18] | Z. A. Maksimović and N. Kovačević, “Preliminary assay on the antioxidative activity of Maydis stigma extracts,” Fitoterapia, vol. 74, no. 1, pp. 144–147, 2003, doi: https://doi.org/10.1016/S0367-326X(02)00311-8. |
| [19] | S. Mohsen and A. Ammar, “Total phenolic contents and antioxidant activity of corn tassel extracts,” Food Chem. - FOOD CHEM, vol. 112, pp. 595–598, Feb. 2009, doi: 10.1016/j.foodchem.2008.06.014. |
| [20] | A. El-Ghorab, K. F. El-Massry, and T. Shibamoto, “Chemical composition of the volatile extract and antioxidant activities of the volatile and nonvolatile extracts of Egyptian corn silk (Zea mays L.).,” J. Agric. Food Chem., vol. 55, no. 22, pp. 9124–9127, Oct. 2007, doi: 10.1021/jf071646e. |
| [21] | J. Guo, T. Liu, L. Han, and Y. Liu, “The effects of corn silk on glycaemic metabolism,” Nutr. Metab. (Lond)., vol. 6, p. 47, Nov. 2009, doi: 10.1186/1743-7075-6-47. |
| [22] | O. Rau, M. Wurglics, T. Dingermann, M. Abdel-Tawab, and M. Schubert-Zsilavecz, “Screening of herbal extracts for activation of the human peroxisome proliferator-activated receptor,” Pharmazie, vol. 61, pp. 952–956, Dec. 2006. |
| [23] | A. C. Waiss Jr. et al., “Maysin, a Flavone Glycoside from Corn Silks with Antibiotic Activity Toward Corn Earworm13,” J. Econ. Entomol., vol. 72, no. 2, pp. 256–258, Apr. 1979, doi: 10.1093/jee/72.2.256. |
| [24] | S. Habtemariam, “Extract of corn silk (stigma of Zea mays) inhibits the tumour necrosis factor-alpha- and bacterial lipopolysaccharide-induced cell adhesion and ICAM-1 expression.,” Planta Med., vol. 64, no. 4, pp. 314–318, May 1998, doi: 10.1055/s-2006-957441. |
| [25] | C. A. Elliger, B. G. Chan, A. C. Waiss, R. E. Lundin, and W. F. Haddon, “C-Glycosylflavones from Zea mays that inhibit insect development,” Phytochemistry, vol. 19, no. 2, pp. 293–297, 1980, doi: https://doi.org/10.1016/S0031-9422(00)81977-9. |
| [26] | M. E. Snook et al., “Levels of maysin and maysin analogs in silks of maize germplasm,” J. Agric. Food Chem., vol. 41, no. 9, pp. 1481–1485, Sep. 1993, doi: 10.1021/jf00033a024. |
| [27] | R. S. Sosa A, Rosa EL, Fusca MDR, “Flavonoids and saponins from styles and stigmas of Zea mays L.(Gramineae),” Acta Farm Bonaer., vol. 16, pp. 215–218, 1997. |
| [28] | N. M. Fazilatun N, Zhari I, “Phytochemicals from corn silk (Zea mays),” J Trop Med Plants, no. 2, pp. 189–92., 2001. |
| [29] | M. A. Ebrahimzadeh, F. Pourmorad, and S. Hafezi, “Antioxidant activities of Iranian corn silk,” Turkish J. Biol., vol. 32, no. 1, pp. 43–49, 2008. |
| [30] | A. Bilia, B. Isacchi, C. Righeschi, C. Guccione, C. Maria, and M. Bergonzi, “Flavonoids Loaded in Nanocarriers: An Opportunity to Increase Oral Bioavailability and Bioefficacy,” Food Nutr. Sci., vol. 05, Jul. 2014, doi: 10.4236/fns.2014.513132. |
| [31] | S. Kumar and A. K. Pandey, “Chemistry and Biological Activities of Flavonoids: An Overview,” Sci. World J., vol. 2013, p. 162750, 2013, doi: 10.1155/2013/162750. |
| [32] | J. Jin, G. E. Sklar, V. Min Sen Oh, and S. Chuen Li, “Factors affecting therapeutic compliance: A review from the patient’s perspective,” Ther. Clin. Risk Manag., vol. 4, no. 1, pp. 269–286, Feb. 2008, doi: 10.2147/tcrm.s1458. |
| [33] | R. S. Solecki, “Shanidar IV, a Neanderthal Flower Burial in Northern Iraq,” Science (80-.)., vol. 190, no. 4217, pp. 880 LP – 881, Nov. 1975, doi: 10.1126/science.190.4217.880. |
| [34] | A. Saklani and S. K. Kutty, “Plant-derived compounds in clinical trials.,” Drug Discov. Today, vol. 13, no. 3–4, pp. 161–171, Feb. 2008, doi: 10.1016/j.drudis.2007.10.010. |
| [35] | F. Shahidi and X. Han, “Encapsulation of food ingredients,” Crit. Rev. Food Sci. Nutr., vol. 33, no. 6, pp. 501–547, Jan. 1993, doi: 10.1080/10408399309527645. |
| [36] | Available from: http://www.apps.who.int/medicinedocs/ en/d/Js7916e/7.12.html., “No Title,” vol. 2015. |
| [37] | E. Marin, M. I. Briceño, and C. Caballero-George, “Critical evaluation of biodegradable polymers used in nanodrugs.,” Int. J. Nanomedicine, vol. 8, pp. 3071–3090, 2013, doi: 10.2147/IJN.S47186. |
| [38] | M. A. Augustin and Y. Hemar, “Nano- and micro-structured assemblies for encapsulation of food ingredients.,” Chem. Soc. Rev., vol. 38, no. 4, pp. 902–912, Apr. 2009, doi: 10.1039/b801739p. |
| [39] | Honkgkee Sah, Melissa Smith, Rey Chern (1996) A novel method of preparing PLGA microcapsules utilizing methylethyl ketone. Pharmaceutical Research, Vol 13, No 3, 1996, 360-365. |
| [40] | Mandal, Sanchita & Kumar, S. & Krishnamoorthy, Balakrishnam & Basu, Sanat. (2010). Development and evaluation of calcium alginate beads prepared by sequential and simultaneous methods. Brazilian Journal of Pharmaceutical Sciences. 46. 785-793. 10.1590/S1984-82502010000400021. |
| [41] | George A. Romar, Thomas S. Kupper, Sherrie J. Divito, Research Techniques Made Simple: Techniques to Assess Cell Proliferation, Journal of Investigative Dermatology, Volume 136, Issue 1, 2016, Pages e1-e7. |
| [42] | Meindl Claudia, Öhlinger Kristin, Ober Jennifer, Roblegg Eva, Fröhlich Eleonore, Comparison of fluorescence-based methods to determine nanoparticle uptake by phagocytes and non-phagocytic cells in vitro, Toxicology, Volume 378, 2017, Pages 25-36. |
| [43] | Fischer AH, Jacobson KA, Rose J, Zeller R. Hematoxylin and eosin staining of tissue and cell sections. CSH Protoc. 2008 May 1; 2008: pdb. prot 4986. doi: 10.1101/pdb.prot4986. PMID: 21356829. |
| [44] | Riss TL, Moravec RA, Niles AL, et al. Cell Viability Assays. 2013 May 1 [Updated 2016 Jul 1]. In: Markossian S, Sittampalam GS, Grossman A, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK144065/. |
| [45] | Rahbar Saadat Y, Saeidi N, Zununi Vahed S, Barzegari A, Barar J. An update to DNA ladder assay for apoptosis detection. Bioimpacts. 2015; 5 (1): 25-28. doi: 10.15171/bi.2015.01 |
| [46] | F. Ahsan, I. P. Rivas, M. A. Khan, and A. I. Torres Suarez, “Targeting to macrophages: role of physicochemical properties of particulate carriers--liposomes and microspheres--on the phagocytosis by macrophages.,” J. Control. Release, vol. 79, no. 1–3, pp. 29–40, Feb. 2002, doi: 10.1016/s0168-3659(01)00549-1. |
| [47] | S. Galindo-Rodriguez, E. Allémann, H. Fessi, and E. Doelker, “Physicochemical parameters associated with nanoparticle formation in the salting-out, emulsification-diffusion, and nanoprecipitation methods.,” Pharm. Res., vol. 21, no. 8, pp. 1428–1439, Aug. 2004, doi: 10.1023/b:pham.0000036917.75634.be. |
| [48] | O. I. Corrigan and X. Li, “Quantifying drug release from PLGA nanoparticulates.,” Eur. J. Pharm. Sci. Off. J. Eur. Fed. Pharm. Sci., vol. 37, no. 3–4, pp. 477–485, Jun. 2009, doi: 10.1016/j.ejps.2009.04.004. |
| [49] | X. Song et al., “Dual agents loaded PLGA nanoparticles: systematic study of particle size and drug entrapment efficiency.,” Eur. J. Pharm. Biopharm. Off. J. Arbeitsgemeinschaft fur Pharm. Verfahrenstechnik e. V, vol. 69, no. 2, pp. 445–453, Jun. 2008, doi: 10.1016/j.ejpb.2008.01.013. |
| [50] | R. M. Mainardes and R. C. Evangelista, “PLGA nanoparticles containing praziquantel: effect of formulation variables on size distribution.,” Int. J. Pharm., vol. 290, no. 1–2, pp. 137–144, Feb. 2005, doi: 10.1016/j.ijpharm.2004.11.027. |
| [51] | X. Song et al., “PLGA nanoparticles simultaneously loaded with vincristine sulfate and verapamil hydrochloride: Systematic study of particle size and drug entrapment efficiency,” Int. J. Pharm., vol. 350, pp. 320–329, Mar. 2008, doi: 10.1016/j.ijpharm.2007.08.034. |
| [52] | M. D. Blanco and M. J. Alonso, “Development and characterization of protein-loaded poly(lactide-co-glycolide) nanospheres,” Eur. J. Pharm. Biopharm., vol. 43, no. 3, pp. 287–294, 1997, doi: https://doi.org/10.1016/S0939-6411(97)00056-8. |
| [53] | C. Gómez-Gaete, N. Tsapis, M. Besnard, A. Bochot, and E. Fattal, “Encapsulation of dexamethasone into biodegradable polymeric nanoparticles.,” Int. J. Pharm., vol. 331, no. 2, pp. 153–159, Mar. 2007, doi: 10.1016/j.ijpharm.2006.11.028. |
APA Style
Prajakta Kulkarni, Madhumita Tawre, Mahesh Dere, Ayesha Khan. (2021). Microencapsulation of Extracts from Corn Hair: A Study on Drug Release and Anticancer Activity. Journal of Drug Design and Medicinal Chemistry, 7(1), 12-22. https://doi.org/10.11648/j.jddmc.20210701.13
ACS Style
Prajakta Kulkarni; Madhumita Tawre; Mahesh Dere; Ayesha Khan. Microencapsulation of Extracts from Corn Hair: A Study on Drug Release and Anticancer Activity. J. Drug Des. Med. Chem. 2021, 7(1), 12-22. doi: 10.11648/j.jddmc.20210701.13
AMA Style
Prajakta Kulkarni, Madhumita Tawre, Mahesh Dere, Ayesha Khan. Microencapsulation of Extracts from Corn Hair: A Study on Drug Release and Anticancer Activity. J Drug Des Med Chem. 2021;7(1):12-22. doi: 10.11648/j.jddmc.20210701.13
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Download@article{10.11648/j.jddmc.20210701.13,
author = {Prajakta Kulkarni and Madhumita Tawre and Mahesh Dere and Ayesha Khan},
title = {Microencapsulation of Extracts from Corn Hair: A Study on Drug Release and Anticancer Activity},
journal = {Journal of Drug Design and Medicinal Chemistry},
volume = {7},
number = {1},
pages = {12-22},
doi = {10.11648/j.jddmc.20210701.13},
url = {https://doi.org/10.11648/j.jddmc.20210701.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jddmc.20210701.13},
abstract = {Corn silk contain inherently substantial flavonoid that contribute to its antioxidant and anticancer activity. The objective of the present study was to fabricate a system for efficient delivery of the anticancer compounds using microencapsulation technique. Methanolic corn silk extract was microencapsulated in the polymer Poly (d,l-lactide-co-glycolide) – PLGA using the solvent extraction method. The physicochemical properties such as size, morphology and physical state of free and encapsulated microparticles were measured by dynamic light scattering, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in vitro release of compounds were studied and quantified using High Performance Liquid Chromatography (HPLC). Spherical and relatively small (d=485.9) polymeric microparticles were obtained containing flavonoids with encapsulation efficiency (EE) of 60.66%. In vitro release profile exhibit a slow, sustained release and follows the first order kinetic with release rate 3.34 x 10-3 m-1s-1. The release characteristics data showed that the drug is released from the microsphere even after 108 h. For in vitro cell-based assays, the MTT cell viability assay was performed on HeLa, NIH 3T3 cell lines while cellular uptake of the drug was studied using fluorescence microscopy. Fluorescence studies confirm drug uptake by the cells within 24 h of treatment. For confirmation of mode of cell death Flow Cytometry and DNA ladder assay was performed. The blank polymeric microparticles were non-toxic to cell while, the drug loaded microparticles exhibit apoptic cell death. Thus an efficient delivery system is achieved after encapsulation, that provides protection and controlled release of the bioactive compounds.},
year = {2021}
}
TY - JOUR T1 - Microencapsulation of Extracts from Corn Hair: A Study on Drug Release and Anticancer Activity AU - Prajakta Kulkarni AU - Madhumita Tawre AU - Mahesh Dere AU - Ayesha Khan Y1 - 2021/03/30 PY - 2021 N1 - https://doi.org/10.11648/j.jddmc.20210701.13 DO - 10.11648/j.jddmc.20210701.13 T2 - Journal of Drug Design and Medicinal Chemistry JF - Journal of Drug Design and Medicinal Chemistry JO - Journal of Drug Design and Medicinal Chemistry SP - 12 EP - 22 PB - Science Publishing Group SN - 2472-3576 UR - https://doi.org/10.11648/j.jddmc.20210701.13 AB - Corn silk contain inherently substantial flavonoid that contribute to its antioxidant and anticancer activity. The objective of the present study was to fabricate a system for efficient delivery of the anticancer compounds using microencapsulation technique. Methanolic corn silk extract was microencapsulated in the polymer Poly (d,l-lactide-co-glycolide) – PLGA using the solvent extraction method. The physicochemical properties such as size, morphology and physical state of free and encapsulated microparticles were measured by dynamic light scattering, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in vitro release of compounds were studied and quantified using High Performance Liquid Chromatography (HPLC). Spherical and relatively small (d=485.9) polymeric microparticles were obtained containing flavonoids with encapsulation efficiency (EE) of 60.66%. In vitro release profile exhibit a slow, sustained release and follows the first order kinetic with release rate 3.34 x 10-3 m-1s-1. The release characteristics data showed that the drug is released from the microsphere even after 108 h. For in vitro cell-based assays, the MTT cell viability assay was performed on HeLa, NIH 3T3 cell lines while cellular uptake of the drug was studied using fluorescence microscopy. Fluorescence studies confirm drug uptake by the cells within 24 h of treatment. For confirmation of mode of cell death Flow Cytometry and DNA ladder assay was performed. The blank polymeric microparticles were non-toxic to cell while, the drug loaded microparticles exhibit apoptic cell death. Thus an efficient delivery system is achieved after encapsulation, that provides protection and controlled release of the bioactive compounds. VL - 7 IS - 1 ER -
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