Antidiabetic, Toxicity Studies and Phytochemical Profile of <i>Hedranthera barteri</i> (Hook. f.) Pichon

Marcus Durojaye Ayoola; Kingsley Onyekachi Ngwu; Oluwaseun Alimot Kareem; Khadijah Adewunmi; Oluwabusayo Emmanuel Kasumu; Akintunde Samuel Odediran; Farouk Adedeji Oladoja; Katherine Olabanjo Olufolabo; Adediwura Fred-Jaiyesimi

doi:doi:10.11648/j.jdmp.20230902.13

Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon

Marcus Durojaye Ayoola, Kingsley Onyekachi Ngwu, Oluwaseun Alimot Kareem, Khadijah Adewunmi, Oluwabusayo Emmanuel Kasumu, Akintunde Samuel Odediran, Farouk Adedeji Oladoja, Katherine Olabanjo Olufolabo, Adediwura Fred-Jaiyesimi

Published in Journal of Diseases and Medicinal Plants (Volume 9, Issue 2)

Received: 23 April 2023 Accepted: 17 May 2023 Published: 29 June 2023

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Objective: This work investigated the antihyperglycaemic activities, safety and chemical composition of Hedranthera barteri (Hook. f.) Pichon (Apocynaceae) methanol leaf extract aimed at establishing its antidiabetic folkloric usage. Methods: Acute and sub acute toxicity tests of the plant extract were assessed with a modified OECD test guidelines. The effects of the extract on glucose, haematological and biochemical parameters were evaluated using standard procedures. Its antidiabetic activities were assayed in α-amylase, α-glucosidase, glucose and streptozotocin-induced diabetic rats’ models at various doses with acarbose and glibenclamide (5 mg/kg) as positive controls. Histopathological examination of the pancreas, liver and kidney of rats administered with the extract at 250, 500 and 1000 mg/kg was also carried out. The phytochemical components of the extract were analysed with GC-MS. The results obtained from these studies were subjected to statistical analysis using Analysis of Variance (ANOVA), followed by the Student Newman Keul's test, and p< 0.05 was taken as significant. Results: The median lethal dose, LD₅₀ of the extract was above 5000 mg/kg in rats without adverse effects on blood glucose levels and biochemical components of normal rats especially at 250 and 500 mg/kg while it significantly potentiated PCV, RBC and haemoglobin levels of the rats. The extract caused an inhibition of α-amylase and α-glucosidase enzymes in concentration dependent manner, reduced hyperglycaemia in glucose loaded rats with 200 mg/kg as the most effective dose and elicited hyperglycaemia lowering activity that was comparable to glibenclamide in drug-induced diabetic rats. The extract had no adverse effect on the histology of the pancreas, liver and kidney at 250 mg/kg while 500 and 1000 mg/kg caused moderate and severe effects on these organs, respectively. Tannins, flavonoids, saponins, alkaloids, terpenoids, deoxy-sugars were observed in the extract by preliminary phytochemical screening while some GC-MS identified possible antidiabetic constituents of the plant include 9, 12-Octadecadinoic acid, phytol and catechol. Conclusion: The study validated the folkloric use of the leaves of Hedranthera barteri in the management of diabetes mellitus and confirmed its non toxic effect on different blood components of animals and their organs at low doses.

Published in	Journal of Diseases and Medicinal Plants (Volume 9, Issue 2)
DOI	10.11648/j.jdmp.20230902.13
Page(s)	49-59
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Hedranthera barteri, Diabetes Mellitus, Toxicity, Phytochemical Profile

References

[1]	Alam S, Hasan MK, Neaz S, Hussain N, Hossain MF, Rahman T. (2021). Diabetes Mellitus: insights from epidemiology, biochemistry, risk factors, diagnosis, complications and comprehensive management. Diabetology, 16 (2): 36-50.
[2]	World Health Organization. Global Report on Diabetes; Geneva, Switzerland, 2017.
[3]	Saeedi P.; Petersohn, I.; Salpea, P.; Malanda, B.; Karuranga, S.; Unwin, N.; Colagiuri, S.; Guariguata, L.; Motala, AA.; Ogurtsova, K.; et al. (2019). Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9th edition. Diabetes Res. Clin. Pract. 157: 107843.
[4]	Lee IM.; Shiroma, EJ; Lobelo, F.; Puska, P. Blair, SN.; Katzmarzyk, PT. (2012). Effect of physical inactivity on major non-communicable diseases worldwide: An analysis of burden of disease and life expectancy. Lancet, 380: 219–229.
[5]	Knott, C.; Bell, S.; Britton, A. Alcohol consumption and the risk of type 2 diabetes (2015): A systematic review and dose-response meta-analysis of more than 1.9 million individuals from 38 observational studies. Diabetes Care, 38: 1804–1812.
[6]	Akter, S.; Goto, A.; Mizoue, T. Smoking and the risk of type 2 diabetes in Japan (2017): A systematic review and meta-analysis. J. Epidemiol. 27: 553–561.
[7]	Danaei, G.; Finucane, MM.; Lu, Y.; Singh, GM.; Cowan, MJ.; Paciorek, CJ.; Lin, JK.; Farzadfar, F.; Khang, Y. H.; Stevens, A. G.; et al. (2011). National, regional, and global trends in fasting plasma glucose and diabetes prevalence since 1980: Systematic analysis of health examination surveys and epidemiological studies with 370 country-years and 2•7 million participants. Lancet 378, 31–40.
[8]	Chen, L.; Chen, R.; Wang, H.; Liang, F. (2015). Mechanisms linking inflammation to insulin resistance. Int. J. Endocrinol. 1–9.
[9]	Burkill, Humphrey M. 1995. 'The useful plants of west tropical Africa, Vols. 1-3', The useful plants of west tropical Africa, Vols. 1-3.
[10]	Thomas, NW. (1910). Report on Edo-spreading people of Nigeria, London. 1640 (Nig. Ser.).
[11]	Thomas, NW. (1967). Awon Ewe Osanyin (Yoruba medicinal leaves), University of Ife, Ile-Ife. 2068 (Nig Ser) K. Verger, P. F.
[12]	Onasanwo, SA. and Elegbe, RA. (2006). Antinociceptive and anti-inflammatory properties of the leaf extracts of Hedranthera barteri in rats and mice. African Journal of Biomedical Research, 9: 109-117.
[13]	William and Li (1970): Alkaloid-bearing plants and their contained alkaloid. Lloydia 33 (3A).
[14]	Onasanwo SA, Chatterjee M, Palit G. (2010a). Antidepressant and anxiolytic potentials of dichloromethane fraction from Hedranthera barteri. Afr J Biomed Res, 13, 76–81.
[15]	Duru CM, and Mbata TI. (2010). The antimicrobial activities and phytochemical screening of ethanolic leaf extracts of Hedranthera barteri Hook and Tabernaemontana pachysiphon Stapf. J Dev Bio Tissue, 2, 1–4.
[16]	Chukwujekwu JC, Staden JV and Smith P. (2005): Antibacterial, anti-inflammatory and antimalarial activities of some Nigerian medicinal plants. South Afr. J. Bot. 71 (3 & 4): 316 – 325.
[17]	Onasanwo SA, Singh N, Olaleye SB, Mishra V, Palit G. (2010b). Antiulcer and antioxidant activities of Hedranthera barteri {(Hook F.) Pichon} with possible involvement of H+, K+ ATPase inhibitory activity. Indian J Med Res, 132, 442–449.
[18]	Sowemimo, A., Odunlami, O. and Fageyinbo, M. (2012). Anticonvulsant activity of Hedranthera barteri, Pharmaceutical Biology, 50: 7, 828-831.
[19]	Bello, OI, Ayoola, MD, Obembe, O. and Akinwunmi, KF. Antidiabetic and Toxicity Studies of the Extract of Four Nigerian Medicinal Plants (2022). European Journal of Medicinal Plants, 33 (11): 32-45.
[20]	OECD. (2001). The OECD guidelines for the testing of chemicals: 423. Acute Oral Toxicity – Acute Toxic Class Method.
[21]	OECD. (2008). The OECD guidelines for the testing of chemicals: 407. Repeated dose 28-day oral toxicity study in rodents.
[22]	Sharma, Hemant, Mauro E Tun-Abraham, and Vivian McAlister. (2019). 'Bidirectional graft-host hematological traffic in liver transplantation', Hepatobiliary Surgery and Nutrition, 8: 253.
[23]	Friedewald, William T, Robert I Levy, and Donald S Fredrickson. 1972. 'Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge', Clinical chemistry, 18: 499-502.
[24]	Baker, Francis Joseph, and Reginald Edward Silverton. (2014). Introduction to medical laboratory technology (Butterworth-Heinemann).
[25]	Sulaimon, L. A., Anise, E. O., Obuotor, E. M., Samuel, A. I. Moshood, T. A., Olajide, M. and Fatoke, T (2020a). In vitro antidiabetic potentials, antioxidant activities and phytochemical profile of African black pepper (Piper guineense). Clinical Phytoscience 6: 1-13.
[26]	Famuyiwa FG, Ayoola, MD, Famuyiwa SO, Aladesanmi AJ. (2018). Hyperglycaemia lowering effect of Kaurane Diterpenoids from the fruits of Xylopia aethiopica (A. Dunal) Rich. International Journal of Medicinal Plants and Natural Products, 4 (3): 11-19.
[27]	Ayoola MD, Adebajo AC, Zotor FB, Pinkoane MG. (2019). Justifying antidiabetic ethnomedicinal claim of Senecio biafrae through its antihyperglycemic and antioxidant activities. Ann Complement Altern Med., 1 (2): 1006.
[28]	Ayoola MD, Oriola AO, Faloye KO, Aladesanmi AJ (2020). Justifying the antidiabetic ethnomedicinal claim of Massularia acuminata through its antihyperglycaemic activity. Am J Biomed Sci & Res., 8 (2): 76- 258.
[29]	Ajileye AJ, Ayoola MD, Elujoba AA, Akinwunmi KF. (2020). Antihyperglycaemic and antioxidant activities of Sansevieria liberica as justification for its antidiabetic claims. Afr. J. Pharm. Pharmacol. 14 (3): 59- 66.
[30]	Faloye, Kolade Olatubosun, Samson Oluwaseyi Famuyiwa, Marcus Durojaye Ayoola, and Derek Tahton Ndinteh. (2020). 'Isolation and Characterization of Two New Polyphenols from the Anti-Diabetic Fraction of Pod Extract of Caesalpinia pulcherrima Swartz', European Journal of Medicinal Plants: 48-56.
[31]	Committee for the update of the guide for the care and use of laboratory animals, institute for laboratory animal research, division on earth and life studies, national research council of the national academies. Guide for the Care and Use of Laboratory Animals, 8th ed. The National Academies Press, Washington, DC. 2011.
[32]	Sunmonu TO, and Oloyede OB. 2010. 'Performance and haematological indices in rats exposed to monocrotophos contamination'. Human & experimental toxicology, 29: 845-50.
[33]	Bashir L, Shittu OK, Rotimi AA, Olalekan IA, Kamooru AA, and Ossai PC. 2015. Effect of Methanol Extract of Telfairia occcidentalis on Haematological Parameters in Wister Rats. J. Med. Sci., 15 (5): 246-250.
[34]	Ojo AO., Oludapo O., Onaseso OO., Adedayo LD., Oluranti OI., Timothy EO., Olubayode, B., Ayoka, A. (2020). Aspilia Africana Altered Haematological Parameters and Plasma Electrolytes in Rats (Rattus norvengicus). Haya Saudi J Life Sci, 5 (5): 58-64.
[35]	Ladokun O., Ojezele M., Arojojoye O. (2015). Comparative study on the effects of aqueous extracts of Viscum album (Mistletoe) from three host plants on hematological parameters in albino rats. African Health Sciences, 15 (2): 606-612.
[36]	Akinwunmi KF, and Ayoola, MD. 2018. Anti-hyperglycaemic, anti-inflammatory and anti-oxidant activities of Carica papaya and Citrullus lanatus seeds. Ife Journal of Science, 20 (2), 207-218.
[37]	Verspohl EJ. 2002. Recommended testing in diabetes research. Planta Med. 68 (7): 581-590.
[38]	Luzi L and Pozza G. 1997. Glibenclamide: An old drug with a novel mechanism of action? Acta Diabetol. 34 (4): 239-244.
[39]	Adebajo AC, Ayoola MD, Obagbemi OR, Obuotor EM, Ogunsina MO, Verspohl EJ. 2013a. Antihyperglycaemic and antioxidant activities of Eugenia uniflora leaf: evaluation of ethnomedical claims IV. Ife J Sci Technol. 1 (1): 1-18.
[40]	Adebajo AC, Ayoola MD, Odediran SA, Aladesanmi AJ, Schmidt TJ, Verspohl EJ. 2013b. Evaluation of ethnomedical claims III: anti-hyperglycaemic activities of Gongronema latifolium root and stem. J Diabetes, (3): 336-43.
[41]	Kar A, Choudhary BK, Bandyopadhyay NG (1999). Preliminary studies on the inorganic constituents of some indigenous hypoglycaemic herbs on oral glucose tolerance test. Journal of Ethnopharmacology, 64 (2): 179- 184.
[42]	Ayoola, M D, Faloye, K O, Famuyiwa, S O, Oyetunde, Y M, Bello, O I, Adewole, A H and Amoo, EA (2022). Antihyperglycaemic Constituents from Cleistopholis patens and Sansevieria liberica as Justification of their Antidiabetic Ethnomedicinal Claims. American Journal of Biomedical Science & Research, 7 (4): 95-101.
[43]	Sulaimon LA, Adisa RA, Obuotor EM, Lawal MO, Moshood AI, Muhammad NH 2020b. Chemical composition, antioxidant, and anticholine esterase activities of essential oil of Xylopia aethiopica seeds. Phcog Res., 12: 112–8.
[44]	Shettar, A. K., Sateesh, M. K., Kaliwal, B. B. Vedamurthy, A. B. 2017. In vitro antidiabetic activities and GC-MS phytochemical analysis of Ximenia americana extracts. South African Journal of Botany, 111: 202-211.
[45]	Kumar, M., Rawat, P., Rahuja, N., Srivastava A., and Maurya, R. 2009. Antihyperglycemic activity of Phenylpropanoyl esters of catechol glycoside and its dimers from Dodecadenia grandiflora. Phytochemistry, 70 (11-12): 1448-1455.

Cite This Article

Plain Text BibTeX RIS

APA Style

Marcus Durojaye Ayoola, Kingsley Onyekachi Ngwu, Oluwaseun Alimot Kareem, Khadijah Adewunmi, Oluwabusayo Emmanuel Kasumu, et al. (2023). Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon. Journal of Diseases and Medicinal Plants, 9(2), 49-59. https://doi.org/10.11648/j.jdmp.20230902.13

Copy | Download

ACS Style

Marcus Durojaye Ayoola; Kingsley Onyekachi Ngwu; Oluwaseun Alimot Kareem; Khadijah Adewunmi; Oluwabusayo Emmanuel Kasumu, et al. Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon. J. Dis. Med. Plants 2023, 9(2), 49-59. doi: 10.11648/j.jdmp.20230902.13

Copy | Download

AMA Style

Marcus Durojaye Ayoola, Kingsley Onyekachi Ngwu, Oluwaseun Alimot Kareem, Khadijah Adewunmi, Oluwabusayo Emmanuel Kasumu, et al. Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon. J Dis Med Plants. 2023;9(2):49-59. doi: 10.11648/j.jdmp.20230902.13

Copy | Download

@article{10.11648/j.jdmp.20230902.13,
  author = {Marcus Durojaye Ayoola and Kingsley Onyekachi Ngwu and Oluwaseun Alimot Kareem and Khadijah Adewunmi and Oluwabusayo Emmanuel Kasumu and Akintunde Samuel Odediran and Farouk Adedeji Oladoja and Katherine Olabanjo Olufolabo and Adediwura Fred-Jaiyesimi},
  title = {Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {9},
  number = {2},
  pages = {49-59},
  doi = {10.11648/j.jdmp.20230902.13},
  url = {https://doi.org/10.11648/j.jdmp.20230902.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20230902.13},
  abstract = {Objective: This work investigated the antihyperglycaemic activities, safety and chemical composition of Hedranthera barteri (Hook. f.) Pichon (Apocynaceae) methanol leaf extract aimed at establishing its antidiabetic folkloric usage. Methods: Acute and sub acute toxicity tests of the plant extract were assessed with a modified OECD test guidelines. The effects of the extract on glucose, haematological and biochemical parameters were evaluated using standard procedures. Its antidiabetic activities were assayed in α-amylase, α-glucosidase, glucose and streptozotocin-induced diabetic rats’ models at various doses with acarbose and glibenclamide (5 mg/kg) as positive controls. Histopathological examination of the pancreas, liver and kidney of rats administered with the extract at 250, 500 and 1000 mg/kg was also carried out. The phytochemical components of the extract were analysed with GC-MS. The results obtained from these studies were subjected to statistical analysis using Analysis of Variance (ANOVA), followed by the Student Newman Keul's test, and pResults: The median lethal dose, LD50 of the extract was above 5000 mg/kg in rats without adverse effects on blood glucose levels and biochemical components of normal rats especially at 250 and 500 mg/kg while it significantly potentiated PCV, RBC and haemoglobin levels of the rats. The extract caused an inhibition of α-amylase and α-glucosidase enzymes in concentration dependent manner, reduced hyperglycaemia in glucose loaded rats with 200 mg/kg as the most effective dose and elicited hyperglycaemia lowering activity that was comparable to glibenclamide in drug-induced diabetic rats. The extract had no adverse effect on the histology of the pancreas, liver and kidney at 250 mg/kg while 500 and 1000 mg/kg caused moderate and severe effects on these organs, respectively. Tannins, flavonoids, saponins, alkaloids, terpenoids, deoxy-sugars were observed in the extract by preliminary phytochemical screening while some GC-MS identified possible antidiabetic constituents of the plant include 9, 12-Octadecadinoic acid, phytol and catechol. Conclusion: The study validated the folkloric use of the leaves of Hedranthera barteri in the management of diabetes mellitus and confirmed its non toxic effect on different blood components of animals and their organs at low doses.},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon
AU  - Marcus Durojaye Ayoola
AU  - Kingsley Onyekachi Ngwu
AU  - Oluwaseun Alimot Kareem
AU  - Khadijah Adewunmi
AU  - Oluwabusayo Emmanuel Kasumu
AU  - Akintunde Samuel Odediran
AU  - Farouk Adedeji Oladoja
AU  - Katherine Olabanjo Olufolabo
AU  - Adediwura Fred-Jaiyesimi
Y1  - 2023/06/29
PY  - 2023
N1  - https://doi.org/10.11648/j.jdmp.20230902.13
DO  - 10.11648/j.jdmp.20230902.13
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 49
EP  - 59
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20230902.13
AB  - Objective: This work investigated the antihyperglycaemic activities, safety and chemical composition of Hedranthera barteri (Hook. f.) Pichon (Apocynaceae) methanol leaf extract aimed at establishing its antidiabetic folkloric usage. Methods: Acute and sub acute toxicity tests of the plant extract were assessed with a modified OECD test guidelines. The effects of the extract on glucose, haematological and biochemical parameters were evaluated using standard procedures. Its antidiabetic activities were assayed in α-amylase, α-glucosidase, glucose and streptozotocin-induced diabetic rats’ models at various doses with acarbose and glibenclamide (5 mg/kg) as positive controls. Histopathological examination of the pancreas, liver and kidney of rats administered with the extract at 250, 500 and 1000 mg/kg was also carried out. The phytochemical components of the extract were analysed with GC-MS. The results obtained from these studies were subjected to statistical analysis using Analysis of Variance (ANOVA), followed by the Student Newman Keul's test, and pResults: The median lethal dose, LD50 of the extract was above 5000 mg/kg in rats without adverse effects on blood glucose levels and biochemical components of normal rats especially at 250 and 500 mg/kg while it significantly potentiated PCV, RBC and haemoglobin levels of the rats. The extract caused an inhibition of α-amylase and α-glucosidase enzymes in concentration dependent manner, reduced hyperglycaemia in glucose loaded rats with 200 mg/kg as the most effective dose and elicited hyperglycaemia lowering activity that was comparable to glibenclamide in drug-induced diabetic rats. The extract had no adverse effect on the histology of the pancreas, liver and kidney at 250 mg/kg while 500 and 1000 mg/kg caused moderate and severe effects on these organs, respectively. Tannins, flavonoids, saponins, alkaloids, terpenoids, deoxy-sugars were observed in the extract by preliminary phytochemical screening while some GC-MS identified possible antidiabetic constituents of the plant include 9, 12-Octadecadinoic acid, phytol and catechol. Conclusion: The study validated the folkloric use of the leaves of Hedranthera barteri in the management of diabetes mellitus and confirmed its non toxic effect on different blood components of animals and their organs at low doses.
VL  - 9
IS  - 2
ER  -

Copy | Download

Author Information

Marcus Durojaye Ayoola

Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria
Kingsley Onyekachi Ngwu

Department of Pharmacognosy, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Nigeria
Oluwaseun Alimot Kareem

Department of Pharmacognosy, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Nigeria
Khadijah Adewunmi

Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria
Oluwabusayo Emmanuel Kasumu

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Nigeria
Akintunde Samuel Odediran

Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria
Farouk Adedeji Oladoja

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Nigeria
Katherine Olabanjo Olufolabo

Department of Pharmacognosy, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Nigeria
Adediwura Fred-Jaiyesimi

Department of Pharmacognosy, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Nigeria

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Marcus Durojaye Ayoola, Kingsley Onyekachi Ngwu, Oluwaseun Alimot Kareem, Khadijah Adewunmi, Oluwabusayo Emmanuel Kasumu, et al. (2023). Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon. Journal of Diseases and Medicinal Plants, 9(2), 49-59. https://doi.org/10.11648/j.jdmp.20230902.13

Copy | Download

ACS Style

Marcus Durojaye Ayoola; Kingsley Onyekachi Ngwu; Oluwaseun Alimot Kareem; Khadijah Adewunmi; Oluwabusayo Emmanuel Kasumu, et al. Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon. J. Dis. Med. Plants 2023, 9(2), 49-59. doi: 10.11648/j.jdmp.20230902.13

Copy | Download

AMA Style

Marcus Durojaye Ayoola, Kingsley Onyekachi Ngwu, Oluwaseun Alimot Kareem, Khadijah Adewunmi, Oluwabusayo Emmanuel Kasumu, et al. Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon. J Dis Med Plants. 2023;9(2):49-59. doi: 10.11648/j.jdmp.20230902.13

Copy | Download

@article{10.11648/j.jdmp.20230902.13,
  author = {Marcus Durojaye Ayoola and Kingsley Onyekachi Ngwu and Oluwaseun Alimot Kareem and Khadijah Adewunmi and Oluwabusayo Emmanuel Kasumu and Akintunde Samuel Odediran and Farouk Adedeji Oladoja and Katherine Olabanjo Olufolabo and Adediwura Fred-Jaiyesimi},
  title = {Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {9},
  number = {2},
  pages = {49-59},
  doi = {10.11648/j.jdmp.20230902.13},
  url = {https://doi.org/10.11648/j.jdmp.20230902.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20230902.13},
  abstract = {Objective: This work investigated the antihyperglycaemic activities, safety and chemical composition of Hedranthera barteri (Hook. f.) Pichon (Apocynaceae) methanol leaf extract aimed at establishing its antidiabetic folkloric usage. Methods: Acute and sub acute toxicity tests of the plant extract were assessed with a modified OECD test guidelines. The effects of the extract on glucose, haematological and biochemical parameters were evaluated using standard procedures. Its antidiabetic activities were assayed in α-amylase, α-glucosidase, glucose and streptozotocin-induced diabetic rats’ models at various doses with acarbose and glibenclamide (5 mg/kg) as positive controls. Histopathological examination of the pancreas, liver and kidney of rats administered with the extract at 250, 500 and 1000 mg/kg was also carried out. The phytochemical components of the extract were analysed with GC-MS. The results obtained from these studies were subjected to statistical analysis using Analysis of Variance (ANOVA), followed by the Student Newman Keul's test, and pResults: The median lethal dose, LD50 of the extract was above 5000 mg/kg in rats without adverse effects on blood glucose levels and biochemical components of normal rats especially at 250 and 500 mg/kg while it significantly potentiated PCV, RBC and haemoglobin levels of the rats. The extract caused an inhibition of α-amylase and α-glucosidase enzymes in concentration dependent manner, reduced hyperglycaemia in glucose loaded rats with 200 mg/kg as the most effective dose and elicited hyperglycaemia lowering activity that was comparable to glibenclamide in drug-induced diabetic rats. The extract had no adverse effect on the histology of the pancreas, liver and kidney at 250 mg/kg while 500 and 1000 mg/kg caused moderate and severe effects on these organs, respectively. Tannins, flavonoids, saponins, alkaloids, terpenoids, deoxy-sugars were observed in the extract by preliminary phytochemical screening while some GC-MS identified possible antidiabetic constituents of the plant include 9, 12-Octadecadinoic acid, phytol and catechol. Conclusion: The study validated the folkloric use of the leaves of Hedranthera barteri in the management of diabetes mellitus and confirmed its non toxic effect on different blood components of animals and their organs at low doses.},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - Antidiabetic, Toxicity Studies and Phytochemical Profile of Hedranthera barteri (Hook. f.) Pichon
AU  - Marcus Durojaye Ayoola
AU  - Kingsley Onyekachi Ngwu
AU  - Oluwaseun Alimot Kareem
AU  - Khadijah Adewunmi
AU  - Oluwabusayo Emmanuel Kasumu
AU  - Akintunde Samuel Odediran
AU  - Farouk Adedeji Oladoja
AU  - Katherine Olabanjo Olufolabo
AU  - Adediwura Fred-Jaiyesimi
Y1  - 2023/06/29
PY  - 2023
N1  - https://doi.org/10.11648/j.jdmp.20230902.13
DO  - 10.11648/j.jdmp.20230902.13
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 49
EP  - 59
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20230902.13
AB  - Objective: This work investigated the antihyperglycaemic activities, safety and chemical composition of Hedranthera barteri (Hook. f.) Pichon (Apocynaceae) methanol leaf extract aimed at establishing its antidiabetic folkloric usage. Methods: Acute and sub acute toxicity tests of the plant extract were assessed with a modified OECD test guidelines. The effects of the extract on glucose, haematological and biochemical parameters were evaluated using standard procedures. Its antidiabetic activities were assayed in α-amylase, α-glucosidase, glucose and streptozotocin-induced diabetic rats’ models at various doses with acarbose and glibenclamide (5 mg/kg) as positive controls. Histopathological examination of the pancreas, liver and kidney of rats administered with the extract at 250, 500 and 1000 mg/kg was also carried out. The phytochemical components of the extract were analysed with GC-MS. The results obtained from these studies were subjected to statistical analysis using Analysis of Variance (ANOVA), followed by the Student Newman Keul's test, and pResults: The median lethal dose, LD50 of the extract was above 5000 mg/kg in rats without adverse effects on blood glucose levels and biochemical components of normal rats especially at 250 and 500 mg/kg while it significantly potentiated PCV, RBC and haemoglobin levels of the rats. The extract caused an inhibition of α-amylase and α-glucosidase enzymes in concentration dependent manner, reduced hyperglycaemia in glucose loaded rats with 200 mg/kg as the most effective dose and elicited hyperglycaemia lowering activity that was comparable to glibenclamide in drug-induced diabetic rats. The extract had no adverse effect on the histology of the pancreas, liver and kidney at 250 mg/kg while 500 and 1000 mg/kg caused moderate and severe effects on these organs, respectively. Tannins, flavonoids, saponins, alkaloids, terpenoids, deoxy-sugars were observed in the extract by preliminary phytochemical screening while some GC-MS identified possible antidiabetic constituents of the plant include 9, 12-Octadecadinoic acid, phytol and catechol. Conclusion: The study validated the folkloric use of the leaves of Hedranthera barteri in the management of diabetes mellitus and confirmed its non toxic effect on different blood components of animals and their organs at low doses.
VL  - 9
IS  - 2
ER  -

Copy | Download