Antioxidant and Antiobesogenic Properties of Aqueous Extracts of Hibiscus sabdariffa, Zingiber officinale and Mentha spicata in Wistar High-Fat Diet Rats

Nkepndep Touohou Suzie Vanissa; Dongho Dongmo Fabrice Fabien; Lienou Lienou Landry; Maffo Tazoho Ghislain; Djeukeu Assongni William; Manz Koule Jules Christophe; Bogning Zangueu Calvin; Mbiatat Hilaire De Goeithe; Ebelle Etame Rebecca Madeleine; Gouado Inocent

doi:doi:10.11648/j.jfns.20221005.11

Antioxidant and Antiobesogenic Properties of Aqueous Extracts of Hibiscus sabdariffa, Zingiber officinale and Mentha spicata in Wistar High-Fat Diet Rats

Nkepndep Touohou Suzie Vanissa, Dongho Dongmo Fabrice Fabien, Lienou Lienou Landry, Maffo Tazoho Ghislain, Djeukeu Assongni William, Manz Koule Jules Christophe, Bogning Zangueu Calvin, Mbiatat Hilaire De Goeithe, Ebelle Etame Rebecca Madeleine, Gouado Inocent

Published in Journal of Food and Nutrition Sciences (Volume 10, Issue 5)

Received: 11 August 2022 Accepted: 15 September 2022 Published: 28 September 2022

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Abstract

The world has already undergone several health-related upheavals that have drawn the attention among which obesity. It is an important public health problem as well in developed as in developing countries. In order to sustainably manage this disease and associated damages, medicinal plants could be an alternative solution to synthetic drugs which can present side effects. Thus, the present study aimed at screening In vitro and In vivo antioxidant and antiobesogenic properties of aqueous extracts of some local plant resources of Cameroon including Hibiscus sabdariffa calyces, Zingiber officinale rhizomes and Mentha spicata leaves. A phytochemical screening was performed on the aqueous extracts of the plants, and their In vitro antioxidant activity evaluated. In vivo antioxidant and antiobesogenic activities were assessed on male Wistar rats. ‘Cafeteria’ diet was used as obesity-inducer and Orlistat as standard treatment. Through the experiment, the extracts were orally administered at 1 g/kg bw per day for 28 days. In vivo antioxidant activity consisted of the evaluation of lipid peroxidation and some enzymes in serum, liver and kidneys. Antiobesogenic properties consisted of determining anthropometric parameters, food consumption pattern, blood lipids profile and glucose content. Hepatic and renal functions were also examined. The screening revealed numerous groups of bioactive compounds in the three tested extracts which also showed reasonable total phenolic content (338.67-1141.12 mgGAE/100g). They revealed excellent antioxidant activity through their good free radical DPPH scavenging potential, reducing power activity, significant decrease of tissue malondialdehyde content and increase of superoxide dismutase and catalase activities. Furthermore, they revealed antiobesogenic effect by their induction of significant reduction (p < 0.05) in body mass index, adipose tissue, food intake, blood contents of triglycerides, total cholesterol, LDL-cholesterol, glucose and atherogenic index, while increasing HDL-cholesterol, all compared to untreated obese animals. They exhibited a relatively protective effect on hepatic and renal functions by remedying histopathological damages caused by high-fat diet and by reducing blood transaminases activity, creatinine and urea contents. These effects were comparable or even greater than those observed with Orlistat. The findings of the present research showed that the three plants could be used in the management of obesity and oxidative stress.

Published in	Journal of Food and Nutrition Sciences (Volume 10, Issue 5)
DOI	10.11648/j.jfns.20221005.11
Page(s)	151-164
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Hibiscus Sabdariffa, Zingiber Officinale, Mentha Spicata, Obesity, Oxidative Stress

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Nkepndep Touohou Suzie Vanissa, Dongho Dongmo Fabrice Fabien, Lienou Lienou Landry, Maffo Tazoho Ghislain, Djeukeu Assongni William, et al. (2022). Antioxidant and Antiobesogenic Properties of Aqueous Extracts of Hibiscus sabdariffa, Zingiber officinale and Mentha spicata in Wistar High-Fat Diet Rats. Journal of Food and Nutrition Sciences, 10(5), 151-164. https://doi.org/10.11648/j.jfns.20221005.11

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Nkepndep Touohou Suzie Vanissa; Dongho Dongmo Fabrice Fabien; Lienou Lienou Landry; Maffo Tazoho Ghislain; Djeukeu Assongni William, et al. Antioxidant and Antiobesogenic Properties of Aqueous Extracts of Hibiscus sabdariffa, Zingiber officinale and Mentha spicata in Wistar High-Fat Diet Rats. J. Food Nutr. Sci. 2022, 10(5), 151-164. doi: 10.11648/j.jfns.20221005.11

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Nkepndep Touohou Suzie Vanissa, Dongho Dongmo Fabrice Fabien, Lienou Lienou Landry, Maffo Tazoho Ghislain, Djeukeu Assongni William, et al. Antioxidant and Antiobesogenic Properties of Aqueous Extracts of Hibiscus sabdariffa, Zingiber officinale and Mentha spicata in Wistar High-Fat Diet Rats. J Food Nutr Sci. 2022;10(5):151-164. doi: 10.11648/j.jfns.20221005.11

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@article{10.11648/j.jfns.20221005.11,
  author = {Nkepndep Touohou Suzie Vanissa and Dongho Dongmo Fabrice Fabien and Lienou Lienou Landry and Maffo Tazoho Ghislain and Djeukeu Assongni William and Manz Koule Jules Christophe and Bogning Zangueu Calvin and Mbiatat Hilaire De Goeithe and Ebelle Etame Rebecca Madeleine and Gouado Inocent},
  title = {Antioxidant and Antiobesogenic Properties of Aqueous Extracts of Hibiscus sabdariffa, Zingiber officinale and Mentha spicata in Wistar High-Fat Diet Rats},
  journal = {Journal of Food and Nutrition Sciences},
  volume = {10},
  number = {5},
  pages = {151-164},
  doi = {10.11648/j.jfns.20221005.11},
  url = {https://doi.org/10.11648/j.jfns.20221005.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20221005.11},
  abstract = {The world has already undergone several health-related upheavals that have drawn the attention among which obesity. It is an important public health problem as well in developed as in developing countries. In order to sustainably manage this disease and associated damages, medicinal plants could be an alternative solution to synthetic drugs which can present side effects. Thus, the present study aimed at screening In vitro and In vivo antioxidant and antiobesogenic properties of aqueous extracts of some local plant resources of Cameroon including Hibiscus sabdariffa calyces, Zingiber officinale rhizomes and Mentha spicata leaves. A phytochemical screening was performed on the aqueous extracts of the plants, and their In vitro antioxidant activity evaluated. In vivo antioxidant and antiobesogenic activities were assessed on male Wistar rats. ‘Cafeteria’ diet was used as obesity-inducer and Orlistat as standard treatment. Through the experiment, the extracts were orally administered at 1 g/kg bw per day for 28 days. In vivo antioxidant activity consisted of the evaluation of lipid peroxidation and some enzymes in serum, liver and kidneys. Antiobesogenic properties consisted of determining anthropometric parameters, food consumption pattern, blood lipids profile and glucose content. Hepatic and renal functions were also examined. The screening revealed numerous groups of bioactive compounds in the three tested extracts which also showed reasonable total phenolic content (338.67-1141.12 mgGAE/100g). They revealed excellent antioxidant activity through their good free radical DPPH scavenging potential, reducing power activity, significant decrease of tissue malondialdehyde content and increase of superoxide dismutase and catalase activities. Furthermore, they revealed antiobesogenic effect by their induction of significant reduction (p < 0.05) in body mass index, adipose tissue, food intake, blood contents of triglycerides, total cholesterol, LDL-cholesterol, glucose and atherogenic index, while increasing HDL-cholesterol, all compared to untreated obese animals. They exhibited a relatively protective effect on hepatic and renal functions by remedying histopathological damages caused by high-fat diet and by reducing blood transaminases activity, creatinine and urea contents. These effects were comparable or even greater than those observed with Orlistat. The findings of the present research showed that the three plants could be used in the management of obesity and oxidative stress.},
 year = {2022}
}

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TY  - JOUR
T1  - Antioxidant and Antiobesogenic Properties of Aqueous Extracts of Hibiscus sabdariffa, Zingiber officinale and Mentha spicata in Wistar High-Fat Diet Rats
AU  - Nkepndep Touohou Suzie Vanissa
AU  - Dongho Dongmo Fabrice Fabien
AU  - Lienou Lienou Landry
AU  - Maffo Tazoho Ghislain
AU  - Djeukeu Assongni William
AU  - Manz Koule Jules Christophe
AU  - Bogning Zangueu Calvin
AU  - Mbiatat Hilaire De Goeithe
AU  - Ebelle Etame Rebecca Madeleine
AU  - Gouado Inocent
Y1  - 2022/09/28
PY  - 2022
N1  - https://doi.org/10.11648/j.jfns.20221005.11
DO  - 10.11648/j.jfns.20221005.11
T2  - Journal of Food and Nutrition Sciences
JF  - Journal of Food and Nutrition Sciences
JO  - Journal of Food and Nutrition Sciences
SP  - 151
EP  - 164
PB  - Science Publishing Group
SN  - 2330-7293
UR  - https://doi.org/10.11648/j.jfns.20221005.11
AB  - The world has already undergone several health-related upheavals that have drawn the attention among which obesity. It is an important public health problem as well in developed as in developing countries. In order to sustainably manage this disease and associated damages, medicinal plants could be an alternative solution to synthetic drugs which can present side effects. Thus, the present study aimed at screening In vitro and In vivo antioxidant and antiobesogenic properties of aqueous extracts of some local plant resources of Cameroon including Hibiscus sabdariffa calyces, Zingiber officinale rhizomes and Mentha spicata leaves. A phytochemical screening was performed on the aqueous extracts of the plants, and their In vitro antioxidant activity evaluated. In vivo antioxidant and antiobesogenic activities were assessed on male Wistar rats. ‘Cafeteria’ diet was used as obesity-inducer and Orlistat as standard treatment. Through the experiment, the extracts were orally administered at 1 g/kg bw per day for 28 days. In vivo antioxidant activity consisted of the evaluation of lipid peroxidation and some enzymes in serum, liver and kidneys. Antiobesogenic properties consisted of determining anthropometric parameters, food consumption pattern, blood lipids profile and glucose content. Hepatic and renal functions were also examined. The screening revealed numerous groups of bioactive compounds in the three tested extracts which also showed reasonable total phenolic content (338.67-1141.12 mgGAE/100g). They revealed excellent antioxidant activity through their good free radical DPPH scavenging potential, reducing power activity, significant decrease of tissue malondialdehyde content and increase of superoxide dismutase and catalase activities. Furthermore, they revealed antiobesogenic effect by their induction of significant reduction (p < 0.05) in body mass index, adipose tissue, food intake, blood contents of triglycerides, total cholesterol, LDL-cholesterol, glucose and atherogenic index, while increasing HDL-cholesterol, all compared to untreated obese animals. They exhibited a relatively protective effect on hepatic and renal functions by remedying histopathological damages caused by high-fat diet and by reducing blood transaminases activity, creatinine and urea contents. These effects were comparable or even greater than those observed with Orlistat. The findings of the present research showed that the three plants could be used in the management of obesity and oxidative stress.
VL  - 10
IS  - 5
ER  -

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Author Information

Nkepndep Touohou Suzie Vanissa

Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
Dongho Dongmo Fabrice Fabien

Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
Lienou Lienou Landry

Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
Maffo Tazoho Ghislain

Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
Djeukeu Assongni William

Department of Home Economic, Advanced Teacher’s Training College for Technical Education, University of Douala, Douala, Cameroon
Manz Koule Jules Christophe

Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
Bogning Zangueu Calvin

Department of Animal Biology and Physiology, Faculty of Sciences, University of Douala, Douala, Cameroon
Mbiatat Hilaire De Goeithe

Services of Catering and Dietetics, Douala General Hospital, Douala, Cameroon
Ebelle Etame Rebecca Madeleine

Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
Gouado Inocent

Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon

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APA Style

Nkepndep Touohou Suzie Vanissa, Dongho Dongmo Fabrice Fabien, Lienou Lienou Landry, Maffo Tazoho Ghislain, Djeukeu Assongni William, et al. (2022). Antioxidant and Antiobesogenic Properties of Aqueous Extracts of Hibiscus sabdariffa, Zingiber officinale and Mentha spicata in Wistar High-Fat Diet Rats. Journal of Food and Nutrition Sciences, 10(5), 151-164. https://doi.org/10.11648/j.jfns.20221005.11

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ACS Style

Nkepndep Touohou Suzie Vanissa; Dongho Dongmo Fabrice Fabien; Lienou Lienou Landry; Maffo Tazoho Ghislain; Djeukeu Assongni William, et al. Antioxidant and Antiobesogenic Properties of Aqueous Extracts of Hibiscus sabdariffa, Zingiber officinale and Mentha spicata in Wistar High-Fat Diet Rats. J. Food Nutr. Sci. 2022, 10(5), 151-164. doi: 10.11648/j.jfns.20221005.11

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AMA Style

Nkepndep Touohou Suzie Vanissa, Dongho Dongmo Fabrice Fabien, Lienou Lienou Landry, Maffo Tazoho Ghislain, Djeukeu Assongni William, et al. Antioxidant and Antiobesogenic Properties of Aqueous Extracts of Hibiscus sabdariffa, Zingiber officinale and Mentha spicata in Wistar High-Fat Diet Rats. J Food Nutr Sci. 2022;10(5):151-164. doi: 10.11648/j.jfns.20221005.11

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@article{10.11648/j.jfns.20221005.11,
  author = {Nkepndep Touohou Suzie Vanissa and Dongho Dongmo Fabrice Fabien and Lienou Lienou Landry and Maffo Tazoho Ghislain and Djeukeu Assongni William and Manz Koule Jules Christophe and Bogning Zangueu Calvin and Mbiatat Hilaire De Goeithe and Ebelle Etame Rebecca Madeleine and Gouado Inocent},
  title = {Antioxidant and Antiobesogenic Properties of Aqueous Extracts of Hibiscus sabdariffa, Zingiber officinale and Mentha spicata in Wistar High-Fat Diet Rats},
  journal = {Journal of Food and Nutrition Sciences},
  volume = {10},
  number = {5},
  pages = {151-164},
  doi = {10.11648/j.jfns.20221005.11},
  url = {https://doi.org/10.11648/j.jfns.20221005.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20221005.11},
  abstract = {The world has already undergone several health-related upheavals that have drawn the attention among which obesity. It is an important public health problem as well in developed as in developing countries. In order to sustainably manage this disease and associated damages, medicinal plants could be an alternative solution to synthetic drugs which can present side effects. Thus, the present study aimed at screening In vitro and In vivo antioxidant and antiobesogenic properties of aqueous extracts of some local plant resources of Cameroon including Hibiscus sabdariffa calyces, Zingiber officinale rhizomes and Mentha spicata leaves. A phytochemical screening was performed on the aqueous extracts of the plants, and their In vitro antioxidant activity evaluated. In vivo antioxidant and antiobesogenic activities were assessed on male Wistar rats. ‘Cafeteria’ diet was used as obesity-inducer and Orlistat as standard treatment. Through the experiment, the extracts were orally administered at 1 g/kg bw per day for 28 days. In vivo antioxidant activity consisted of the evaluation of lipid peroxidation and some enzymes in serum, liver and kidneys. Antiobesogenic properties consisted of determining anthropometric parameters, food consumption pattern, blood lipids profile and glucose content. Hepatic and renal functions were also examined. The screening revealed numerous groups of bioactive compounds in the three tested extracts which also showed reasonable total phenolic content (338.67-1141.12 mgGAE/100g). They revealed excellent antioxidant activity through their good free radical DPPH scavenging potential, reducing power activity, significant decrease of tissue malondialdehyde content and increase of superoxide dismutase and catalase activities. Furthermore, they revealed antiobesogenic effect by their induction of significant reduction (p < 0.05) in body mass index, adipose tissue, food intake, blood contents of triglycerides, total cholesterol, LDL-cholesterol, glucose and atherogenic index, while increasing HDL-cholesterol, all compared to untreated obese animals. They exhibited a relatively protective effect on hepatic and renal functions by remedying histopathological damages caused by high-fat diet and by reducing blood transaminases activity, creatinine and urea contents. These effects were comparable or even greater than those observed with Orlistat. The findings of the present research showed that the three plants could be used in the management of obesity and oxidative stress.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Antioxidant and Antiobesogenic Properties of Aqueous Extracts of Hibiscus sabdariffa, Zingiber officinale and Mentha spicata in Wistar High-Fat Diet Rats
AU  - Nkepndep Touohou Suzie Vanissa
AU  - Dongho Dongmo Fabrice Fabien
AU  - Lienou Lienou Landry
AU  - Maffo Tazoho Ghislain
AU  - Djeukeu Assongni William
AU  - Manz Koule Jules Christophe
AU  - Bogning Zangueu Calvin
AU  - Mbiatat Hilaire De Goeithe
AU  - Ebelle Etame Rebecca Madeleine
AU  - Gouado Inocent
Y1  - 2022/09/28
PY  - 2022
N1  - https://doi.org/10.11648/j.jfns.20221005.11
DO  - 10.11648/j.jfns.20221005.11
T2  - Journal of Food and Nutrition Sciences
JF  - Journal of Food and Nutrition Sciences
JO  - Journal of Food and Nutrition Sciences
SP  - 151
EP  - 164
PB  - Science Publishing Group
SN  - 2330-7293
UR  - https://doi.org/10.11648/j.jfns.20221005.11
AB  - The world has already undergone several health-related upheavals that have drawn the attention among which obesity. It is an important public health problem as well in developed as in developing countries. In order to sustainably manage this disease and associated damages, medicinal plants could be an alternative solution to synthetic drugs which can present side effects. Thus, the present study aimed at screening In vitro and In vivo antioxidant and antiobesogenic properties of aqueous extracts of some local plant resources of Cameroon including Hibiscus sabdariffa calyces, Zingiber officinale rhizomes and Mentha spicata leaves. A phytochemical screening was performed on the aqueous extracts of the plants, and their In vitro antioxidant activity evaluated. In vivo antioxidant and antiobesogenic activities were assessed on male Wistar rats. ‘Cafeteria’ diet was used as obesity-inducer and Orlistat as standard treatment. Through the experiment, the extracts were orally administered at 1 g/kg bw per day for 28 days. In vivo antioxidant activity consisted of the evaluation of lipid peroxidation and some enzymes in serum, liver and kidneys. Antiobesogenic properties consisted of determining anthropometric parameters, food consumption pattern, blood lipids profile and glucose content. Hepatic and renal functions were also examined. The screening revealed numerous groups of bioactive compounds in the three tested extracts which also showed reasonable total phenolic content (338.67-1141.12 mgGAE/100g). They revealed excellent antioxidant activity through their good free radical DPPH scavenging potential, reducing power activity, significant decrease of tissue malondialdehyde content and increase of superoxide dismutase and catalase activities. Furthermore, they revealed antiobesogenic effect by their induction of significant reduction (p < 0.05) in body mass index, adipose tissue, food intake, blood contents of triglycerides, total cholesterol, LDL-cholesterol, glucose and atherogenic index, while increasing HDL-cholesterol, all compared to untreated obese animals. They exhibited a relatively protective effect on hepatic and renal functions by remedying histopathological damages caused by high-fat diet and by reducing blood transaminases activity, creatinine and urea contents. These effects were comparable or even greater than those observed with Orlistat. The findings of the present research showed that the three plants could be used in the management of obesity and oxidative stress.
VL  - 10
IS  - 5
ER  -

Copy | Download