Cucumber green mottle mosaic virus (CGMMV) is one of the most important viruses affecting Cucurbitaceae crops. It has been found all over the world. Once spread, it may cause significant loss on economic crops. During a project from 2019 to 2021, occurrence of Cucumber green mottle mosaic virus (CGMMV) on main leguminosae crops (Pisum sativum, Vicia faba, Vigna unguiculata, Glycine max, Phaseolus vulgaris) was surveyed via random sampling in Ningbo, Zhejiang, China. Leaf chlorosis, yellowing (or whitening), shrinkage, spots and deformation were observed and sampled accordingly. Altogether 47 legume leaf samples were collected from 10 locations during different seasons. Total RNA was analyzed through real-time RT-PCR. Among 141 RNA extractions (3 repetitive extractions for each sample) tested, 15 were positive (corresponding to 9 samples, Ct<35), and 126 negative. Nine positive samples were further verified by conventional RT-PCR and sequencing. BLAST analysis showed that 7 sequences were over 98% identical to MP and CP regions of CGMMV isolate from Guangdong, China (Accession: MK933286), while the other 2 were over 97% identical to MP and CP regions of CGMMV isolate from Zhejiang, China (Accession: KM873783). Among 47 samples, 9 (19.15%) were positive, and out of 141 nucleic acid extractions, 15 were positive, accounting for 10.64%. Inoculation test on legume seedlings (V. faba var. Beidouqixing and P. sativum var. Jingpindawuxu) was carried out using infectious cDNA clone of CGMMV (pCB-CGMMV). Stunted growth, leaf deformation, chlorosis, and whitening were observed on both V. faba and P. sativum at 25 dpi (days post inoculation). Symptomatic leaves were diagnosed by real-time RT-PCR, which turned out to be CGMMV positive. It is speculated that leguminous plants are alternative hosts of CGMMV under natural conditions, and can serve as "virus reservoir" when cucurbitaceae plants are rare. Background research on economic significant pests is supposed to be conducted regularly. On one hand, it contributes to control of agricultural diseases and reduction of related losses. On the other, it would provide basic data for follow-up researches and supplement information for important pests.
| Published in | Journal of Plant Sciences (Volume 9, Issue 6) |
| DOI | 10.11648/j.jps.20210906.15 |
| Page(s) | 305-308 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2021. Published by Science Publishing Group |
Cucumber green mottle mosaic virus, Leguminous Crops, Host Range, Disease Research
| [1] | Dombrovsky, A., Tran-Nguyen, L. T. T., & Jones, R. A. C. (2017) Cucumber green mottle mosaic virus: Rapidly Increasing Global Distribution, Etiology, Epidemiology, and Management. Annual Review of Phytopathology, 55, 231-256. |
| [2] | Chen, J., & Li, M. F. (2007). The new intruder--Cucumber green mottle mosaic virus. Plant Quarantine, 2 (21): 94-96. |
| [3] | Wu, Y. H., Li, L. M., Zhao, X. X., Wang, W. H., Wang, L., Cai, M. (2010). Risk analysis of colonization and spread of Cucumber green mottle mosaic virus in China. Plant Protection, 36 (1): 33-36. |
| [4] | Yoshimi, O. (1986). Cucumber green mottle mosaic virus. The plant viruses, Springer, Boston, MA, 267-281. |
| [5] | Blattný, C., & Janecková, M. (1979). Apricot bare twig and unfruitfulness. International Symposium on Fruit Tree Virus Diseases, 94, 383-390. |
| [6] | Antignus, Y., Pearlsman, M., Ben-Yoseph, R., & Cohen, S. (1990). Occurrence of a variant of cucumber green mottle mosaic virus in ISRAEL. Phytoparasitica, 18 (1): 50-56. |
| [7] | Boubourakas, I. N., Hatziloukas, E., Antignus, Y., & Katis, N. I. (2010). Etiology of leaf chlorosis and deterioration of the fruit interior of watermelon plants. Journal of Phytopathology, 152 (10): 580-588. |
| [8] | Valery, P., Irena, B., Tetyana, S., & Svitlana, O. (2010). Evidence for plant viruses in the region of argentina islands, antarctica. Fems Microbiology Ecology, 2, 409-417. |
| [9] | Shargil, D., Smith, E., Lachman, O., Reingold, V., & Dombrovsky, A. (2017). New weed hosts for cucumber green mottle mosaic virus in wild mediterranean vegetation. European Journal of Plant Pathology, 148: 473-480. |
| [10] | Chen, H., Zhao, W., Gu, Q., Chen, Q., Lin, S., & Zhu, S. (2008). Real time taqman rt-pcr assay for the detection of cucumber green mottle mosaic virus. Journal of Virological Methods, 149 (2): 326-329. |
| [11] | Huang, J., Liao, F. R., Lin, S. M., Chen, Q., & Wang, Z. H. (2007). Identification and detection of Cucumber green mottle mosaic virus. Chinese Agricultural Science Bulletin, 23 (4): 318-322. |
| [12] | Zheng, H. Y., Xiao, C. L., Han, K. L., Peng, J. J., & Lin, L. (2015). Development of an agroinoculation system for full-length and GFP-tagged cDNA clones of cucumber green mottle mosaic virus. Archives of Virology, 160 (11): 2867-2872. |
| [13] | Varveri, C., Vassilakos, N., & Bem, F. (2002). Characterization and detection of cucumber green mottle mosaic virus in greece. Phytoparasitica, 30 (5): 493-501. |
| [14] | Chanda, B., Shamimuzzaman, M., Gilliard, A., & Ling, K. S. (2021). Effectiveness of disinfectants against the spread of tobamoviruses: tomato brown rugose fruit virus and cucumber green mottle mosaic virus. Virology Journal, 18 (7): https://doi.org/10.1186/s12985-020-01479-8. |
| [15] | Wu, H. J., Qin, B. X., Chen, H. Y., Peng, B., Cai, J. H., & Gu, Q. S. (2011). The Rate of Seed Contamination and Transmission of Cucumber green mottle mosaic virus in Watermelon and Melon. Scientia Agricultura Sinica, 44 (7): 1527-1532. |
| [16] | Zhao, H. R., Lin, Z. Y., Huang, Y., Qu, J. L. & Gao, B. D. (2011). The First Report of Cucumber Green Mottle Mosaic Virus in Hunan Province. Chinese Society of Plant Pathology, Annual Conference, 1-311. |
APA Style
Wang Jiaying, Cui Junxia, Zhang Jihong, Zhao Xiuling, Li Wen, et al. (2021). Research on Infectivity of Cucumber Green Mottle Mosaic Virus on Important Leguminous Crops. Journal of Plant Sciences, 9(6), 305-308. https://doi.org/10.11648/j.jps.20210906.15
ACS Style
Wang Jiaying; Cui Junxia; Zhang Jihong; Zhao Xiuling; Li Wen, et al. Research on Infectivity of Cucumber Green Mottle Mosaic Virus on Important Leguminous Crops. J. Plant Sci. 2021, 9(6), 305-308. doi: 10.11648/j.jps.20210906.15
AMA Style
Wang Jiaying, Cui Junxia, Zhang Jihong, Zhao Xiuling, Li Wen, et al. Research on Infectivity of Cucumber Green Mottle Mosaic Virus on Important Leguminous Crops. J Plant Sci. 2021;9(6):305-308. doi: 10.11648/j.jps.20210906.15
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Download@article{10.11648/j.jps.20210906.15,
author = {Wang Jiaying and Cui Junxia and Zhang Jihong and Zhao Xiuling and Li Wen and Chen Xianfeng},
title = {Research on Infectivity of Cucumber Green Mottle Mosaic Virus on Important Leguminous Crops},
journal = {Journal of Plant Sciences},
volume = {9},
number = {6},
pages = {305-308},
doi = {10.11648/j.jps.20210906.15},
url = {https://doi.org/10.11648/j.jps.20210906.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20210906.15},
abstract = {Cucumber green mottle mosaic virus (CGMMV) is one of the most important viruses affecting Cucurbitaceae crops. It has been found all over the world. Once spread, it may cause significant loss on economic crops. During a project from 2019 to 2021, occurrence of Cucumber green mottle mosaic virus (CGMMV) on main leguminosae crops (Pisum sativum, Vicia faba, Vigna unguiculata, Glycine max, Phaseolus vulgaris) was surveyed via random sampling in Ningbo, Zhejiang, China. Leaf chlorosis, yellowing (or whitening), shrinkage, spots and deformation were observed and sampled accordingly. Altogether 47 legume leaf samples were collected from 10 locations during different seasons. Total RNA was analyzed through real-time RT-PCR. Among 141 RNA extractions (3 repetitive extractions for each sample) tested, 15 were positive (corresponding to 9 samples, CtV. faba var. Beidouqixing and P. sativum var. Jingpindawuxu) was carried out using infectious cDNA clone of CGMMV (pCB-CGMMV). Stunted growth, leaf deformation, chlorosis, and whitening were observed on both V. faba and P. sativum at 25 dpi (days post inoculation). Symptomatic leaves were diagnosed by real-time RT-PCR, which turned out to be CGMMV positive. It is speculated that leguminous plants are alternative hosts of CGMMV under natural conditions, and can serve as "virus reservoir" when cucurbitaceae plants are rare. Background research on economic significant pests is supposed to be conducted regularly. On one hand, it contributes to control of agricultural diseases and reduction of related losses. On the other, it would provide basic data for follow-up researches and supplement information for important pests.},
year = {2021}
}
TY - JOUR T1 - Research on Infectivity of Cucumber Green Mottle Mosaic Virus on Important Leguminous Crops AU - Wang Jiaying AU - Cui Junxia AU - Zhang Jihong AU - Zhao Xiuling AU - Li Wen AU - Chen Xianfeng Y1 - 2021/12/09 PY - 2021 N1 - https://doi.org/10.11648/j.jps.20210906.15 DO - 10.11648/j.jps.20210906.15 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 305 EP - 308 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20210906.15 AB - Cucumber green mottle mosaic virus (CGMMV) is one of the most important viruses affecting Cucurbitaceae crops. It has been found all over the world. Once spread, it may cause significant loss on economic crops. During a project from 2019 to 2021, occurrence of Cucumber green mottle mosaic virus (CGMMV) on main leguminosae crops (Pisum sativum, Vicia faba, Vigna unguiculata, Glycine max, Phaseolus vulgaris) was surveyed via random sampling in Ningbo, Zhejiang, China. Leaf chlorosis, yellowing (or whitening), shrinkage, spots and deformation were observed and sampled accordingly. Altogether 47 legume leaf samples were collected from 10 locations during different seasons. Total RNA was analyzed through real-time RT-PCR. Among 141 RNA extractions (3 repetitive extractions for each sample) tested, 15 were positive (corresponding to 9 samples, CtV. faba var. Beidouqixing and P. sativum var. Jingpindawuxu) was carried out using infectious cDNA clone of CGMMV (pCB-CGMMV). Stunted growth, leaf deformation, chlorosis, and whitening were observed on both V. faba and P. sativum at 25 dpi (days post inoculation). Symptomatic leaves were diagnosed by real-time RT-PCR, which turned out to be CGMMV positive. It is speculated that leguminous plants are alternative hosts of CGMMV under natural conditions, and can serve as "virus reservoir" when cucurbitaceae plants are rare. Background research on economic significant pests is supposed to be conducted regularly. On one hand, it contributes to control of agricultural diseases and reduction of related losses. On the other, it would provide basic data for follow-up researches and supplement information for important pests. VL - 9 IS - 6 ER -
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