Phytochemical, Nutritional and Antimicrobial Evaluations of the Aqueous Extract of Brassica Nigra (Brassicaceae) Seeds
American Journal of Applied Chemistry
Volume 4, Issue 4, August 2016, Pages: 161-163
Received: Sep. 1, 2015;
Accepted: Apr. 1, 2016;
Published: Aug. 3, 2016
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Uzama Danlami, Chemistry Advanced Research Centre, Sheda Science and Technology Complex, Garki, Abuja, Nigeria
Orishadipe Abayomi T, Chemistry Advanced Research Centre, Sheda Science and Technology Complex, Garki, Abuja, Nigeria
Danhalilu Rabiu Lawal, Department of Science Laboratory Technology, Federal Polytechnic Kaura Bnamoda, Zamfara State, Nigeria
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The objective of the study was to carry out phytochemical, nutritional and antimicrobial evaluations of the aqueous extract of Brassica nigra seeds. The nutritional and phytochemical analyses were carried out by adopting standard methods. The antimicrobial study was carried out by using agar well diffusion method. The nutritional evaluation of the seeds of Brassica nigra showed that it contained moisture 4.16%, crude fibre 0.30%, crude fat 30.30%, ash 5.14%, crude protein 24.70% and carbohydrate 35.40%. The phytochemical screening of the seeds indicated the presence of saponins, alkaloids, flavonoids, glycosides, reducing sugar, phlobatannins and volatile oil. It was observed that the extract was effective in inhibiting Escherichia coli, Klebsiella pneumonia, Salmonella para-typhi, Pseudomonas aeuriginosa and Staphylococcus aureus, with the zone of inhibition ranging between 7mm and 23mm. The extracts of the Brassica nigra seeds can be used for common cold, painful joints and muscles (rheumatism), arthritis, edema, and increasing appetite. The seeds can also serve as a source of cooking oil because of the high content of crude fat and ethno medicine.
Brassica Nigra, Proximate Analysis, Phytochemicals, Reducing Sugar, Common Cold, Volatile Oil, Antimicrobial
To cite this article
Orishadipe Abayomi T,
Danhalilu Rabiu Lawal,
Phytochemical, Nutritional and Antimicrobial Evaluations of the Aqueous Extract of Brassica Nigra (Brassicaceae) Seeds, American Journal of Applied Chemistry.
Vol. 4, No. 4,
2016, pp. 161-163.
Zemede A., conservation and uses of traditional vegetables in Ethiopia, proceedings of IPGRI International workshop on Genetic Resources of traditional vegetables in Africa (Nairobi) 29th – 31st August, 1995.
Sokman A., Jones B. and Erturk M., Antimicrobial activity of extracts from cell cultures of some Turkish medicinal plants. Phytotherapy Research, 1999; 13: 355-357.
Gomezde Saravia S. G. and Gaylarde C. C., The antimicrobial activity of an extract of Brassica nigra. Biodet. Biodegrad. 1998; 41: 145-148.
Heidari M. R. and Abbsifard M., Evaluation of the analgesic effect of Brassica nigra in mice. J. Mazandaran Univ. Med. Sci, 2002; 12: 18-26.
Zahra K., Mohsen K., Mehrdad R. and Azam S., Antiepileptic and Antioxidant effect of Brassica nigra on Pentyene tertrazol- induced kindly in mice, Iran J. Pharm. Res., 2012; 11 (4): 1209–1217.
Aiyaa S. J., Antibacterial activity of oil extracted of Brassica nigra seeds on some bacteria isolated from plaque and healthy teeth in children (1-5) years. Basrah J. Sci. 2012; 30 (1): 105–119.
AOAC Official Method of Analysis, 13th edition, William Horwitz edition. Washington DC. Association of Official Analytical Chemist. 1980; 7: 56–132.
Uzama D., Phytochemical screening and antibacterial activity of Guava (Psidiumguajava L.) crude extracts. Bio. Env. Sci. J. Trop. 2009; 6 (4): 139–142.
Sofowora L. A., Medicinal plants and traditional medicine in Africa. Spectrum Books Ltd. Ibadan, Harbone, 1993, 55-71.
Perez C., Paul M and Bazerque P., Antibiotic assay by the agar well diffusion method. Acta. Biomed. Exp. 1990, 15: 113-115.
Uzama D., Fagbohun, A. A., Bwai M. D., Danhalilu R. L. and Kabir M. G. Proximate and Phytochemical analyses of the hexane, ethyl acetateand ethanol extracts of Chromolaenaodoratum leaves, Asian J. Pharm. & Bio. Res. 2013; 3 (1): 34–35.
Nwaogu L. A., Ujuwundu C. O. and Mgbemena A. I., Studies on theNutritional and phytochemical composition of Amaratushybridus leaves, Bis-Res. 2000; 4: 28-31.
Asibey-Berko E. and Tayle F. A. K., Proximate analysis of some under utilized Ghanian Vegetables. Ghana J. Sci, 1999; 39: 91-92.
Basha N. S., The invitro anthelmintic activities of Brassica nigra, Ocimum basilicun And Rumex, phytotherapy research, 2011; 13: 421–423.