Isolation, Structural Elucidation, and Bioactivity Studies of Leaf Extract of Vernonia Amygdalina

Muluye Melak Zenebe; Bitew Kassaw Dessie; Getachew G/Mariam W/Hana; Adhena Ayaliew Werkneh

doi:doi:10.11648/j.ajac.20150301.13

| Peer-Reviewed

Isolation, Structural Elucidation, and Bioactivity Studies of Leaf Extract of Vernonia Amygdalina

Muluye Melak Zenebe, Bitew Kassaw Dessie, Getachew G/Mariam W/Hana, Adhena Ayaliew Werkneh

Published in American Journal of Applied Chemistry (Volume 3, Issue 1)

Received: 1 January 2015 Accepted: 10 January 2015 Published: 21 January 2015

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Vernonia Amygdalina (VA) is a tropical African plant of the Asteraceae family and is occasionally cultivated for its medicinal uses, which include as a treatment for diarrhea, skin wounds, fever mastitis and worms infection. The aim of this study is to isolate and elucidate the structure of some of the chemical constituents from the leaf of Vernonia Amygdalina and its bioactivities. The methanol extract of Vernonia Amygdalina, after repeated chromatography led to the isolation of a different compound, one of this compound (MM-4) is partially characterized based on the spectral data (IR, 1H NMR, 13C NMR, and DEPT) and extracts from Vernonia Amygdalina has been shown to have antimicrobial activity.

Published in	American Journal of Applied Chemistry (Volume 3, Issue 1)
DOI	10.11648/j.ajac.20150301.13
Page(s)	14-20
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Vernonia Amygdalina, Bioactivity, Phytochemical Investigation

References

[1]	Thomson, R. H. (Ed.). (1993). The chemistry of natural products. Springer.
[2]	Khalafalla, M. M., Daffalla, H. M., El-Shemy, H. A., & Abdellatef, E. (2009). Establishment of in vitro fast-growing normal root culture of Vernonia amygdalina-a potent African medicinal plant. African Journal of Biotechnology, 8(21).
[3]	Erasto, P., Grierson, D. S., &Afolayan, A. J. (2006). Bioactive sesquiterpene lactones from the leaves of< i> Vernonia amygdalina. Journal of ethnopharmacology, 106(1), 117-120.
[4]	Havsteen, B. H. (2002). The biochemistry and medical significance of the flavonoids. Pharmacology & therapeutics, 96(2), 67-202.
[5]	Heim, K. E., Tagliaferro, A. R., &Bobilya, D. J. (2002). Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. The Journal of nutritional biochemistry, 13(10), 572-584.
[6]	Eyong, E. U., Agiang, M. A., Atangwho, I. J., Iwara, I. A., Odey, M. O., &Ebong, P. E. (2011). Phytochemicals and micronutrients composition of root and stem bark extracts of Vernonia amygdalina Del. Journal of Medicine and Medical Science, 2(6), 900-903.
[7]	Awe, S. O., Makinde, J. M., &Olajide, O. A. (1999). Cathartic effect of the leaf extract of Vernonia amygdalina. Fitoterapia, 70(2), 161-165.
[8]	Oboh, F. O. J., &Enobhayisobo, E. I.(2009). Effect of aqueous extract of Vernonia amygdalina leaves on plasma lipids of hyperlipidaemic adult male albino New Zealand rabbits, 10 (4).
[9]	Yeap, S. K., Ho, W. Y., Beh, B. K., Liang, W. S., Ky, H., Yousr, A. H. N., &Alitheen, N. B. (2010). Vernonia amygdalina, an ethnoveterinary and ethnomedical used green vegetable with multiple bioactivities. J Medicinal Plants Res, 4, 2787-2812.
[10]	Ijeh, I. I., &Ejike, C. E. (2011). Current perspectives on the medicinal potentials of Vernonia amygdalina Del. Journal of Medicinal Plants Research, 5(7), 1051-1061.
[11]	Atangwho, I. J., Ebong, P. E., Eyong, E. U., &Eteng, M. U. (2010). Combined administration of extracts of Vernonia amygdalina (Del) and Azadirachtaindica (A. Juss) mimic insulin in time-course body weight and glucose regulation in diabetic and non-diabetic rats. Nigerian Journal of Biochemistry and Molecular Biology, 25(1), 44-49.
[12]	Asawalam, E. F., Emosairue, S. O., &Hassanali, A. (2008). Contribution of different constituents to the toxicity of the essential oil constituents of Vernonia amygdalina (Compositae) and Xylopiaaetiopica (Annonaceae) on maize weevil Sitophilus zeamaisMotschulsky (Coleoptera: Curculionidae). African Journal of Biotechnology, 7(16).
[13]	Oboh, G. (2006). Nutritive value and haemolytic properties (in vitro) of the leaves of Vernonia amygdalina on human erythrocyte. Nutrition and health, 18(2), 151-160.Alawa, C. B. I., Adamu, A. M., Gefu, J. O., Ajanusi, O. J.,
[14]	Abdu, P. A., Chiezey, N. P., & Bowman, D. D. (2003). In vitro screening of two Nigerian medicinal plants Vernonia amygdalina and Annona senegalensisfor anthelmintic activity. Veterinary Parasitology, 113(1), 73-81.
[15]	Oyugi, D. A., Ayorinde, F. O., Gugssa, A., Allen, A., Izevbigie, E. B., Eribo, B., & Anderson, W. A. (2011). Biological activity and mass spectrometric analysis of Vernonia amygdalina fractions. J Biosci Tech, 2(3), 287-304.
[16]	Ayoola, G. A., Coker, H. A., Adesegun, S. A., Adepoju-Bello, A. A., Obaweya, K., Ezennia, E. C., & Atangbayila, T. O. (2008). Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria. Tropical Journal of Pharmaceutical Research, 7(3), 1019-1024.
[17]	Oboh, F. O., & Masodje, H. I. (2009). Nutritional and antimicrobial properties of Vernonia amygdalina leaves. Int. J. Biomed & Hlth. Sci, 5(2).
[18]	Erasto, P., Grierson, D. S., & Afolayan, A. J. (2007). Evaluation of antioxidant activity and the fatty acid profile of the leaves of Vernonia amygdalina growing in South Africa. Food chemistry, 104(2), 636-642.

Cite This Article

Plain Text BibTeX RIS

APA Style

Muluye Melak Zenebe, Bitew Kassaw Dessie, Getachew G/Mariam W/Hana, Adhena Ayaliew Werkneh. (2015). Isolation, Structural Elucidation, and Bioactivity Studies of Leaf Extract of Vernonia Amygdalina. American Journal of Applied Chemistry, 3(1), 14-20. https://doi.org/10.11648/j.ajac.20150301.13

Copy | Download

ACS Style

Muluye Melak Zenebe; Bitew Kassaw Dessie; Getachew G/Mariam W/Hana; Adhena Ayaliew Werkneh. Isolation, Structural Elucidation, and Bioactivity Studies of Leaf Extract of Vernonia Amygdalina. Am. J. Appl. Chem. 2015, 3(1), 14-20. doi: 10.11648/j.ajac.20150301.13

Copy | Download

AMA Style

Muluye Melak Zenebe, Bitew Kassaw Dessie, Getachew G/Mariam W/Hana, Adhena Ayaliew Werkneh. Isolation, Structural Elucidation, and Bioactivity Studies of Leaf Extract of Vernonia Amygdalina. Am J Appl Chem. 2015;3(1):14-20. doi: 10.11648/j.ajac.20150301.13

Copy | Download

@article{10.11648/j.ajac.20150301.13,
  author = {Muluye Melak Zenebe and Bitew Kassaw Dessie and Getachew G/Mariam W/Hana and Adhena Ayaliew Werkneh},
  title = {Isolation, Structural Elucidation, and Bioactivity Studies of Leaf Extract of Vernonia Amygdalina},
  journal = {American Journal of Applied Chemistry},
  volume = {3},
  number = {1},
  pages = {14-20},
  doi = {10.11648/j.ajac.20150301.13},
  url = {https://doi.org/10.11648/j.ajac.20150301.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20150301.13},
  abstract = {Vernonia Amygdalina (VA) is a tropical African plant of the Asteraceae family and is occasionally cultivated for its medicinal uses, which include as a treatment for diarrhea, skin wounds, fever mastitis and worms infection. The aim of this study is to isolate and elucidate the structure of some of the chemical constituents from the leaf of Vernonia Amygdalina and its bioactivities. The methanol extract of Vernonia Amygdalina, after repeated chromatography led to the isolation of a different compound, one of this compound (MM-4) is partially characterized based on the spectral data (IR, 1H NMR, 13C NMR, and DEPT) and extracts from Vernonia Amygdalina has been shown to have antimicrobial activity.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Isolation, Structural Elucidation, and Bioactivity Studies of Leaf Extract of Vernonia Amygdalina
AU  - Muluye Melak Zenebe
AU  - Bitew Kassaw Dessie
AU  - Getachew G/Mariam W/Hana
AU  - Adhena Ayaliew Werkneh
Y1  - 2015/01/21
PY  - 2015
N1  - https://doi.org/10.11648/j.ajac.20150301.13
DO  - 10.11648/j.ajac.20150301.13
T2  - American Journal of Applied Chemistry
JF  - American Journal of Applied Chemistry
JO  - American Journal of Applied Chemistry
SP  - 14
EP  - 20
PB  - Science Publishing Group
SN  - 2330-8745
UR  - https://doi.org/10.11648/j.ajac.20150301.13
AB  - Vernonia Amygdalina (VA) is a tropical African plant of the Asteraceae family and is occasionally cultivated for its medicinal uses, which include as a treatment for diarrhea, skin wounds, fever mastitis and worms infection. The aim of this study is to isolate and elucidate the structure of some of the chemical constituents from the leaf of Vernonia Amygdalina and its bioactivities. The methanol extract of Vernonia Amygdalina, after repeated chromatography led to the isolation of a different compound, one of this compound (MM-4) is partially characterized based on the spectral data (IR, 1H NMR, 13C NMR, and DEPT) and extracts from Vernonia Amygdalina has been shown to have antimicrobial activity.
VL  - 3
IS  - 1
ER  -

Copy | Download

Author Information

Muluye Melak Zenebe

Department of Chemistry, College of Natural and Computational Science, University of Gondar, Gondar, Ethiopia
Bitew Kassaw Dessie

Department of Chemistry, College of Natural and Computational Science, University of Gondar, Gondar, Ethiopia
Getachew G/Mariam W/Hana

Department of Chemistry, College of Natural and Computational Science, University of Gondar, Gondar, Ethiopia
Adhena Ayaliew Werkneh

Department of Chemistry, College of Natural Science, Jigjiga University, Jigjiga, Ethiopia

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Muluye Melak Zenebe, Bitew Kassaw Dessie, Getachew G/Mariam W/Hana, Adhena Ayaliew Werkneh. (2015). Isolation, Structural Elucidation, and Bioactivity Studies of Leaf Extract of Vernonia Amygdalina. American Journal of Applied Chemistry, 3(1), 14-20. https://doi.org/10.11648/j.ajac.20150301.13

Copy | Download

ACS Style

Muluye Melak Zenebe; Bitew Kassaw Dessie; Getachew G/Mariam W/Hana; Adhena Ayaliew Werkneh. Isolation, Structural Elucidation, and Bioactivity Studies of Leaf Extract of Vernonia Amygdalina. Am. J. Appl. Chem. 2015, 3(1), 14-20. doi: 10.11648/j.ajac.20150301.13

Copy | Download

AMA Style

Muluye Melak Zenebe, Bitew Kassaw Dessie, Getachew G/Mariam W/Hana, Adhena Ayaliew Werkneh. Isolation, Structural Elucidation, and Bioactivity Studies of Leaf Extract of Vernonia Amygdalina. Am J Appl Chem. 2015;3(1):14-20. doi: 10.11648/j.ajac.20150301.13

Copy | Download

@article{10.11648/j.ajac.20150301.13,
  author = {Muluye Melak Zenebe and Bitew Kassaw Dessie and Getachew G/Mariam W/Hana and Adhena Ayaliew Werkneh},
  title = {Isolation, Structural Elucidation, and Bioactivity Studies of Leaf Extract of Vernonia Amygdalina},
  journal = {American Journal of Applied Chemistry},
  volume = {3},
  number = {1},
  pages = {14-20},
  doi = {10.11648/j.ajac.20150301.13},
  url = {https://doi.org/10.11648/j.ajac.20150301.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20150301.13},
  abstract = {Vernonia Amygdalina (VA) is a tropical African plant of the Asteraceae family and is occasionally cultivated for its medicinal uses, which include as a treatment for diarrhea, skin wounds, fever mastitis and worms infection. The aim of this study is to isolate and elucidate the structure of some of the chemical constituents from the leaf of Vernonia Amygdalina and its bioactivities. The methanol extract of Vernonia Amygdalina, after repeated chromatography led to the isolation of a different compound, one of this compound (MM-4) is partially characterized based on the spectral data (IR, 1H NMR, 13C NMR, and DEPT) and extracts from Vernonia Amygdalina has been shown to have antimicrobial activity.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Isolation, Structural Elucidation, and Bioactivity Studies of Leaf Extract of Vernonia Amygdalina
AU  - Muluye Melak Zenebe
AU  - Bitew Kassaw Dessie
AU  - Getachew G/Mariam W/Hana
AU  - Adhena Ayaliew Werkneh
Y1  - 2015/01/21
PY  - 2015
N1  - https://doi.org/10.11648/j.ajac.20150301.13
DO  - 10.11648/j.ajac.20150301.13
T2  - American Journal of Applied Chemistry
JF  - American Journal of Applied Chemistry
JO  - American Journal of Applied Chemistry
SP  - 14
EP  - 20
PB  - Science Publishing Group
SN  - 2330-8745
UR  - https://doi.org/10.11648/j.ajac.20150301.13
AB  - Vernonia Amygdalina (VA) is a tropical African plant of the Asteraceae family and is occasionally cultivated for its medicinal uses, which include as a treatment for diarrhea, skin wounds, fever mastitis and worms infection. The aim of this study is to isolate and elucidate the structure of some of the chemical constituents from the leaf of Vernonia Amygdalina and its bioactivities. The methanol extract of Vernonia Amygdalina, after repeated chromatography led to the isolation of a different compound, one of this compound (MM-4) is partially characterized based on the spectral data (IR, 1H NMR, 13C NMR, and DEPT) and extracts from Vernonia Amygdalina has been shown to have antimicrobial activity.
VL  - 3
IS  - 1
ER  -

Copy | Download