Physicochemical Characterization and Pesticide Residue Analysis of Honey Produced in West Shewa Zone, Oromia Region, Ethiopia
American Journal of Applied Chemistry
Volume 5, Issue 6, December 2017, Pages: 101-109
Received: Oct. 9, 2017; Accepted: Oct. 30, 2017; Published: Dec. 8, 2017
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Eyobel Mulugeta, Department of Chemistry, Ambo University, Ambo, Ethiopia
Wodaje Addis, Department of Chemistry, Ambo University, Ambo, Ethiopia
Lemessa Benti, Department of Chemistry, Ambo University, Ambo, Ethiopia
Miresa Tadese, Department of Chemistry, Ambo University, Ambo, Ethiopia
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This study investigated physicochemical properties and pesticide residue levels of honey from West Shewa Zone, Ethiopia. The results of moisture, electrical conductivity, pH, free acidity, reducing sugar, sucrose, ash, and total solids were found to be 16.61–18.64%, 0.384–0.646 mS/cm, 3.77–4.22, 7.42–13.87 meq/kg, 61.38–72.87%, 6.84–15.94%, 0.030–0.095%, and 81.36–83.28%, respectively. Correlation analysis has shown moderate positive correlation at P < 0.01 between sucrose and ash (r = 0.555), and a strong negative correlation between moisture and total solids (r = -0.9171). Residues of 4,4-DDD, 4,4-DDT, Dieldrin, α-Endosulfan, and β-Endosulfan were detected, among 20 organochlorine pesticides analyzed. Most physicochemical parameters have been found within the acceptable range set by national and international standards except sucrose content from Ejere and Toke Kutaye. The estimated daily intakes (EDIs) of pesticides identified in samples were below acceptable daily intakes (ADIs), that indicate honey ingesting has a negligible influence to toxicological risk.
Honey, Physicochemical Properties, Honey Quality, Pesticide Residues, Estimated Daily Intakes
To cite this article
Eyobel Mulugeta, Wodaje Addis, Lemessa Benti, Miresa Tadese, Physicochemical Characterization and Pesticide Residue Analysis of Honey Produced in West Shewa Zone, Oromia Region, Ethiopia, American Journal of Applied Chemistry. Vol. 5, No. 6, 2017, pp. 101-109. doi: 10.11648/j.ajac.20170506.13
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This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Adenekan, M. O.; Amusa, N. A.; Lawal, A. O.; Okpeze, V. E., Physico-chemical and Microbiological Properties of Honey Samples Obtained from Ibadan. Journal of Microbiology and Antimicrobials 2010, 2, 100–104.
Kavapurayil, J. B.; Karalam, S.; Chandran, R. P., Analysis of Physicochemical, Biochemical, and Antibacterial Properties of Indian Honey Samples with Special Reference to their Non-conformity. Acta Alimentaria 2014, 43, 9–18.
Awad, M. H.; Elgornazi, A. M., Physicochemical Characterization of Honey from Kasr Khiar and Garaboli Areas-Libya. Asian Journal of Plant Science and Research 2016, 6, 8–12.
Kowsalya, V., Antibacterial Activity of Honey and Erytlaria Acualis against Bacteria Isolated from Burnt Wound Sepsis. IOSR Journal of Pharmacy and Biological Sciences 2012, 1, 1–2.
Omoya, F. O.; Ijabadeniyi, O. A.; Ogonnoh, O. B., Physicochemical Properties of Honey Samples from Ondo State, Nigeria, and their Bioactivity against Spoilage and Pathogenic Organisms. Journal of Food, Agriculture and Environment 2014, 12, 104–107.
Delphine, D. N.; Joseph, T., Palynological and Physicochemical Characterization of Honey in the Sudano-Guinean Zone of Cameroon. Food and Nutrition Sciences 2015, 6, 1339–1350.
Gairola, A.; Tiwari, P.; Tiwari, J. K., Physico-chemical Properties of Apis Cerana-indica F. Honey from Uttarkashi District of Uttarakhand, India. Journal of Global Biosciences 2013, 2, 20–25.
Gomes, S.; Dias, L. G.; Moreira, L. L.; Rodrigues, P.; Estevinho, L., Physicochemical, Microbiological and Antimicrobial Properties of Commercial Honeys from Portugal. Food and Chemical Toxicology 2010, 48, 544–548.
Awraris, G.; Hailemariam, G.; Dejen, A.; Zerihun, T., Physico-chemical Properties of Honey Produced in Masha, Gesha, and Sheko Districts in South-western Ethiopia. Current Research in Agricultural Sciences 2014, 1, 110–116.
Kayode, J.; Oyeyemi, S. D., Physico-chemical Investigation of Honey Samples from Bee Farmers in Ekiti State, Southwest Nigeria. Journal of Plant Sciences 2014, 2, 246–249.
Oyeyemi, S. D.; Kayode, J.; Owolabi, M. O., Comparative Nutritional Studies on Honey Samples in Ado Ekiti, Ekiti State, Nigeria. Donnish Journal of Medicinal Plant Research 2015, 2, 016–020.
El-Sohaimy, S. A.; Masry, S. H. D.; Shehata, M. G., Physicochemical Characteristics of Honey from Different Origins. Annals of Agricultural Sciences 2015, 60, 279–287.
Belay, A.; Solomon, W. K.; Bultossa, G.; Adgaba, N.; Melaku, S., Physicochemical Properties of the Harenna Forest Honey, Bale, Ethiopia. Food Chemistry 2013, 141, 3386–3392.
Blasco, C.; Vazquez-Roig, P.; Onghena, M.; Masia, A.; Picó, Y., Analysis of Insecticides in Honey by Liquid Chromatography–ion Trap-mass Spectrometry: Comparison of Different Extraction Procedures. Journal of Chromatography A 2011, 1218, 4892–4901.
European Union Reference Laboratory (EURL), Pesticides Residues in Food of Animal Origin and Commodities with High Fat Content, European Commission, Freiburg, Germany, 2012.
Gangwar, S. K.; Gebremariam, H.; Ebrahim, A.; Tajebe, S., Characteristics of Honey Produced by Different Plant Species in Ethiopia. Advances in Bioresearch 2010, 1, 101–105.
Kebede, N; Subramanian, P. A.; Gebrekidan, M., Physicochemical Analysis of Tigray Honey: An Attempt to Determine Major Quality Markets of Honey. Bulletin of the Chemical Society of Ethiopia 2012, 26, 127–133.
Sisay, G.; Eyasu S.; Amsalu, B., Physicochemical Properties of Honey Produced in the Homesha District of Western Ethiopia. Journal of Apicultural Science 2012, 56, 33–40.
Tewodros, A.; Eyassu, S.; Amsalu, B., Physicochemical Properties of Honey Produced in Sekota District, Northern Ethiopia. International Food Research Journal 2013, 20, 3061–3067.
Tadesse, G.; Gebregziabher, B., Determination of Quality and Adulteration Effects of Honey from Adigrat and its Surrounding Areas. International Journal of Technology Enhancements and Emerging Engineering Research 2014, 2, 71–76.
Gebru, E.; Berhanu, A.; Hayal, L.; Solomon, A., Physicochemical Characterization of Honey from Debre-Nazret Kebele of Tigray Region, Ethiopia. World Applied Sciences Journal 2015, 33, 1806–1814.
Bekele, T.; Desalegn, B.; Mitiku, E., Evaluation of Physico-chemical Properties of Honey Produced in Bale Natural Forest, Southeastern Ethiopia. International Journal of Agricultural Science and Food Technology 2016, 2, 021–027.
Agbagwa, O. E.; Otokunefor, T. V.; Nnenna, F. P., Quality Assessment of Nigeria Honey and Manuka Honey. Journal of Microbiology and Biotechnology Research 2011, 1, 20–31.
Saxena, S.; Gautam, S.; Sharma, A., Physical, Biochemical and Antioxidant Properties of some Indian Honeys. Food Chemistry 2010, 118, 391–397.
Akbulut, M.; Ozcan, M. M.; Coklar, H., Evaluation of Antioxidant Activity, Phenolic, Mineral Contents and some Physicochemical Properties of Several Pine Honeys Collected from Western Anatolia. International Journal of Food Science and Nutrition 2009, 60, 577–589.
Ozcan, M. M.; Tastepe, B.; Arslan, D.; Unver, A., Some Qualitative Properties of Different Monofloral Honeys. Journal of Agroalimentary Processes and Technologies 2013, 19, 355–361.
AOAC, Association of Official Analytical Chemists (16th ed). Official Methods of Analysis, 962.19: Washington, DC, 1999.
Codex Alimentarius. Revised Codex Standard for Honey, Codex Stan. 12–1981, Rev. 1 (1987), Rev. 2 (2001), 2001, 12, 1–7.
Bogdanov, S.; Martine, P.; Lullmann C., Harmonized Methods of the International Honey Commission. Apidologie 1997, 1-59.
Lehotay S. J., Determination of Pesticide Residues in Foods by Acetonitrile Extraction and Partitioning with Magnesium Sulfate: Collaborative Study. Journal of Association Official Analytical Chemists International 2007, 90, 485–520.
European Union (EU). Council Directive 2001/110/EC of 20th, December 2001 relating to honey. Official Journal of the European Communities, L10, 47–52, 2002.
Ethiopia Standard (ES). Honey Specification: Ethiopian Standard, ES 1202:2005, First edition. Addis Ababa, Ethiopia, 2005.
Finola, M. S.; Lasagno, M. C.; Marioli, J. M., Microbiological and Chemical Characterization of Honeys from Central Argentina. Food Chemistry 2007, 100, 1649–1653.
Reshma, M. V.; Shyma, S.; George, T. M.; Rishin, A. V.; Ravi, K. C.; Shilu, L., Study on the Physicochemical Parameters, Phenolic Profile and Antioxidant Properties of Indian Honey Samples from Extrafloral Sources and Multi Floral Sources. International Food Research Journal 2016, 23, 2021–2028.
Moniruzzaman, M.; Khalil, M. I.; Sulaiman, S. A.; Gan, S. H., Physicochemical and Antioxidant Properties of Malaysian Honeys Produced by Apis Cerana, Apis Dorsata and Apis Mellifera. BMC Complementary and Alternative Medicine 2013, 13, 1–12.
Nascimento, A. S.; Marchini, L. C.; Carvalho, C. A. L.; Araújo, D. F. D.; Olinda, R. A.; Silveira, T. A., Physical-chemical Parameters of Honey of Stingless Bee (Hymenoptera: Apidae). American Chemical Science Journal 2015, 7, 139–149.
Terrab, A.; Diez, M. J.; Heredia, F. J., Characterization of Moroccan Unifloral Honeys by their Physicochemical Characteristics. Food Chemistry 2002, 79, 373–379.
Kumar, H. M.; Ananda, A. P.; Vishwanathan, D.; Siddagangaiah, D., Study of Physicochemical Parameters and Antioxidant in Honey Collected from Different Locations of India. International Journal of Pharmacy and Life Sciences 2013, 4, 3159–3165.
Nanda, V.; Sarkar, B. C.; Sharmaa, H. K.; Bawa, A. S., Physicochemical Properties and Estimation of Mineral Content in Honey Produced from Different Plants in Northern India. Journal of Food Composition and Analysis 2003, 16, 613–619.
Admasu, A.; Nuru, A., Effect of Honeybee Pollination on Seed Yield and Oil Content of Niger (Guizotia abyssinica): Proceedings of the First National Conference of Ethiopian Beekeepers Association, June 7-8, 1999, Addis Ababa, Ethiopia, 2000, 67–73.
Manzoor, M.; Mathivanan, V.; Shah, G. N.; Mir, G. M.; Selvisabhanayakam. Physico-chemical Analysis of Honey of Apis Cerana Indica and Apis Mellifera from Different Regions of Anantnag District, Jammu and Kashmir. International Journal of Pharmacy and Pharmaceutical Sciences 2013, 5, 635–638.
Lawal, R. A.; Lawal, A. K.; Adekalu, J. B., Physicochemical Studies on Adulteration of Honey in Nigeria. Pakistan Journal of Biological Sciences 2009, 12, 1080–1084.
World Health Organization (WHO), Guidelines for Predicting Dietary Intake of Pesticide Residues (revised). Global Environment Monitoring System- Food Contamination and Assessment Programme (GEMS/Food) in Collaboration with Codex Committee on Pesticide Residues, 1997.
Food and Agriculture Organization (FAO), Submission and Evaluation of Pesticide Residues Data for the Estimation of Maximum Residue Levels in Food and Feed (1st ed.). Rome, 2002.
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