HPLC-DAD and HPLC-ESI-MS Analysis of Polyphenol-rich Extracts from Mango (<i>Mangifera indica</i> L.), Tommy Atkins and Haden Varieties, Cultivated in Dominican Republic

Alberto Julio Nunez-Selles; Victor Manuel Espaillat Martínez; Lauro Nuevas Paz

doi:doi:10.11648/j.ijpc.20200606.12

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HPLC-DAD and HPLC-ESI-MS Analysis of Polyphenol-rich Extracts from Mango (Mangifera indica L.), Tommy Atkins and Haden Varieties, Cultivated in Dominican Republic

Alberto Julio Nunez-Selles, Victor Manuel Espaillat Martínez, Lauro Nuevas Paz

Received: 25 November 2020 Accepted: 4 December 2020 Published: 16 December 2020

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Abstract

Background: Antioxidant, anti-inflammatory, and analgesic effects of mango stem bark extracts (MSBE) have been reported. A previous published report described the identification of several components (polyphenols, polyols, and sugars) by HPLC, MS, and NMR. Mangiferin (2-β-D-glucopyranosyl-1,3,6,7-tetrahydroxyl-9H- xanthen-9-one) was identified as the major bioactive component of MSBE. However, MSBE has shown more potent effects than model mangiferin solutions in several in vitro experiments at different concentrations. Therefore, there are other extract components which contribute to the observed pharmacological effects. Objective: The identification of other polyphenolic components in MSBE, which may contribute to the observed pharmacological effects through a synergic pathway. Method: Polyphenol-rich extracts from mango stem bark and branch trees from two varieties (Haden and Tommy Atkins), cultivated in Dominican Republic, were analyzed by HPLC-DAD. Butanol MSBE extract from Haden mango stem bark was analyzed by HPLC-MS-ESI. Results: The mangiferin content in Haden mango extracts by HPLC-DAD was considerably high as compared to previous reports in the literature from other mango varieties. The identification of Haden mango stem bark butanolic extract components by HPLC-ESI-MS led to the unambiguous identification of 20 components: 2 benzoic acid derivatives, gallic acid and 8 gallate derivatives, 2 benzophenones (maclaurin and iriflophenone types), 3 flavonoids (catechin, epicatechin, and quercetin), mangiferin, isomangiferin, homomangiferin, and noratyriol. Conclusion: HPLC-ESI-MS analysis of the Haden mango SB extract showed the presence of many polyphenolic components, not previously reported, which may correlate to the antioxidant, anti-inflammatory, and/or analgesic effects of this extract mostly through a synergistic effect of its components. The potential exploitation of mango by-products from the Haden variety would be the best option for obtaining polyphenol-rich extracts from mango agricultural by-products to be used as bioactive ingredients in nutraceutical, cosmeceutical, and/or pharmaceutical formulations.

DOI	10.11648/j.ijpc.20200606.12
Published in	International Journal of Pharmacy and Chemistry (Volume 6, Issue 6, November 2020)
Page(s)	77-88
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Mangiferin, Mangifera indica, Haden Mango, Tommy Atkins Mango, Stem Bark, Branch Tree, Circular Economy

References

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Alberto Julio Nunez-Selles, Victor Manuel Espaillat Martínez, Lauro Nuevas Paz. (2020). HPLC-DAD and HPLC-ESI-MS Analysis of Polyphenol-rich Extracts from Mango (Mangifera indica L.), Tommy Atkins and Haden Varieties, Cultivated in Dominican Republic. International Journal of Pharmacy and Chemistry, 6(6), 77-88. https://doi.org/10.11648/j.ijpc.20200606.12

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Alberto Julio Nunez-Selles; Victor Manuel Espaillat Martínez; Lauro Nuevas Paz. HPLC-DAD and HPLC-ESI-MS Analysis of Polyphenol-rich Extracts from Mango (Mangifera indica L.), Tommy Atkins and Haden Varieties, Cultivated in Dominican Republic. Int. J. Pharm. Chem. 2020, 6(6), 77-88. doi: 10.11648/j.ijpc.20200606.12

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Alberto Julio Nunez-Selles, Victor Manuel Espaillat Martínez, Lauro Nuevas Paz. HPLC-DAD and HPLC-ESI-MS Analysis of Polyphenol-rich Extracts from Mango (Mangifera indica L.), Tommy Atkins and Haden Varieties, Cultivated in Dominican Republic. Int J Pharm Chem. 2020;6(6):77-88. doi: 10.11648/j.ijpc.20200606.12

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@article{10.11648/j.ijpc.20200606.12,
  author = {Alberto Julio Nunez-Selles and Victor Manuel Espaillat Martínez and Lauro Nuevas Paz},
  title = {HPLC-DAD and HPLC-ESI-MS Analysis of Polyphenol-rich Extracts from Mango (Mangifera indica L.), Tommy Atkins and Haden Varieties, Cultivated in Dominican Republic},
  journal = {International Journal of Pharmacy and Chemistry},
  volume = {6},
  number = {6},
  pages = {77-88},
  doi = {10.11648/j.ijpc.20200606.12},
  url = {https://doi.org/10.11648/j.ijpc.20200606.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20200606.12},
  abstract = {Background: Antioxidant, anti-inflammatory, and analgesic effects of mango stem bark extracts (MSBE) have been reported. A previous published report described the identification of several components (polyphenols, polyols, and sugars) by HPLC, MS, and NMR. Mangiferin (2-β-D-glucopyranosyl-1,3,6,7-tetrahydroxyl-9H- xanthen-9-one) was identified as the major bioactive component of MSBE. However, MSBE has shown more potent effects than model mangiferin solutions in several in vitro experiments at different concentrations. Therefore, there are other extract components which contribute to the observed pharmacological effects. Objective: The identification of other polyphenolic components in MSBE, which may contribute to the observed pharmacological effects through a synergic pathway. Method: Polyphenol-rich extracts from mango stem bark and branch trees from two varieties (Haden and Tommy Atkins), cultivated in Dominican Republic, were analyzed by HPLC-DAD. Butanol MSBE extract from Haden mango stem bark was analyzed by HPLC-MS-ESI. Results: The mangiferin content in Haden mango extracts by HPLC-DAD was considerably high as compared to previous reports in the literature from other mango varieties. The identification of Haden mango stem bark butanolic extract components by HPLC-ESI-MS led to the unambiguous identification of 20 components: 2 benzoic acid derivatives, gallic acid and 8 gallate derivatives, 2 benzophenones (maclaurin and iriflophenone types), 3 flavonoids (catechin, epicatechin, and quercetin), mangiferin, isomangiferin, homomangiferin, and noratyriol. Conclusion: HPLC-ESI-MS analysis of the Haden mango SB extract showed the presence of many polyphenolic components, not previously reported, which may correlate to the antioxidant, anti-inflammatory, and/or analgesic effects of this extract mostly through a synergistic effect of its components. The potential exploitation of mango by-products from the Haden variety would be the best option for obtaining polyphenol-rich extracts from mango agricultural by-products to be used as bioactive ingredients in nutraceutical, cosmeceutical, and/or pharmaceutical formulations.},
 year = {2020}
}

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TY  - JOUR
T1  - HPLC-DAD and HPLC-ESI-MS Analysis of Polyphenol-rich Extracts from Mango (Mangifera indica L.), Tommy Atkins and Haden Varieties, Cultivated in Dominican Republic
AU  - Alberto Julio Nunez-Selles
AU  - Victor Manuel Espaillat Martínez
AU  - Lauro Nuevas Paz
Y1  - 2020/12/16
PY  - 2020
N1  - https://doi.org/10.11648/j.ijpc.20200606.12
DO  - 10.11648/j.ijpc.20200606.12
T2  - International Journal of Pharmacy and Chemistry
JF  - International Journal of Pharmacy and Chemistry
JO  - International Journal of Pharmacy and Chemistry
SP  - 77
EP  - 88
PB  - Science Publishing Group
SN  - 2575-5749
UR  - https://doi.org/10.11648/j.ijpc.20200606.12
AB  - Background: Antioxidant, anti-inflammatory, and analgesic effects of mango stem bark extracts (MSBE) have been reported. A previous published report described the identification of several components (polyphenols, polyols, and sugars) by HPLC, MS, and NMR. Mangiferin (2-β-D-glucopyranosyl-1,3,6,7-tetrahydroxyl-9H- xanthen-9-one) was identified as the major bioactive component of MSBE. However, MSBE has shown more potent effects than model mangiferin solutions in several in vitro experiments at different concentrations. Therefore, there are other extract components which contribute to the observed pharmacological effects. Objective: The identification of other polyphenolic components in MSBE, which may contribute to the observed pharmacological effects through a synergic pathway. Method: Polyphenol-rich extracts from mango stem bark and branch trees from two varieties (Haden and Tommy Atkins), cultivated in Dominican Republic, were analyzed by HPLC-DAD. Butanol MSBE extract from Haden mango stem bark was analyzed by HPLC-MS-ESI. Results: The mangiferin content in Haden mango extracts by HPLC-DAD was considerably high as compared to previous reports in the literature from other mango varieties. The identification of Haden mango stem bark butanolic extract components by HPLC-ESI-MS led to the unambiguous identification of 20 components: 2 benzoic acid derivatives, gallic acid and 8 gallate derivatives, 2 benzophenones (maclaurin and iriflophenone types), 3 flavonoids (catechin, epicatechin, and quercetin), mangiferin, isomangiferin, homomangiferin, and noratyriol. Conclusion: HPLC-ESI-MS analysis of the Haden mango SB extract showed the presence of many polyphenolic components, not previously reported, which may correlate to the antioxidant, anti-inflammatory, and/or analgesic effects of this extract mostly through a synergistic effect of its components. The potential exploitation of mango by-products from the Haden variety would be the best option for obtaining polyphenol-rich extracts from mango agricultural by-products to be used as bioactive ingredients in nutraceutical, cosmeceutical, and/or pharmaceutical formulations.
VL  - 6
IS  - 6
ER  -

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Alberto Julio Nunez-Selles

Research Division, National Evangelic University (UNEV), Santo Domingo, Dominican Republic
Victor Manuel Espaillat Martínez

Research Division, National Evangelic University (UNEV), Santo Domingo, Dominican Republic
Lauro Nuevas Paz

R&D Department, Laboratories Magnachem, San Cristóbal, Dominican Republic

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Alberto Julio Nunez-Selles, Victor Manuel Espaillat Martínez, Lauro Nuevas Paz. (2020). HPLC-DAD and HPLC-ESI-MS Analysis of Polyphenol-rich Extracts from Mango (Mangifera indica L.), Tommy Atkins and Haden Varieties, Cultivated in Dominican Republic. International Journal of Pharmacy and Chemistry, 6(6), 77-88. https://doi.org/10.11648/j.ijpc.20200606.12

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Alberto Julio Nunez-Selles; Victor Manuel Espaillat Martínez; Lauro Nuevas Paz. HPLC-DAD and HPLC-ESI-MS Analysis of Polyphenol-rich Extracts from Mango (Mangifera indica L.), Tommy Atkins and Haden Varieties, Cultivated in Dominican Republic. Int. J. Pharm. Chem. 2020, 6(6), 77-88. doi: 10.11648/j.ijpc.20200606.12

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Alberto Julio Nunez-Selles, Victor Manuel Espaillat Martínez, Lauro Nuevas Paz. HPLC-DAD and HPLC-ESI-MS Analysis of Polyphenol-rich Extracts from Mango (Mangifera indica L.), Tommy Atkins and Haden Varieties, Cultivated in Dominican Republic. Int J Pharm Chem. 2020;6(6):77-88. doi: 10.11648/j.ijpc.20200606.12

Copy | Download

@article{10.11648/j.ijpc.20200606.12,
  author = {Alberto Julio Nunez-Selles and Victor Manuel Espaillat Martínez and Lauro Nuevas Paz},
  title = {HPLC-DAD and HPLC-ESI-MS Analysis of Polyphenol-rich Extracts from Mango (Mangifera indica L.), Tommy Atkins and Haden Varieties, Cultivated in Dominican Republic},
  journal = {International Journal of Pharmacy and Chemistry},
  volume = {6},
  number = {6},
  pages = {77-88},
  doi = {10.11648/j.ijpc.20200606.12},
  url = {https://doi.org/10.11648/j.ijpc.20200606.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20200606.12},
  abstract = {Background: Antioxidant, anti-inflammatory, and analgesic effects of mango stem bark extracts (MSBE) have been reported. A previous published report described the identification of several components (polyphenols, polyols, and sugars) by HPLC, MS, and NMR. Mangiferin (2-β-D-glucopyranosyl-1,3,6,7-tetrahydroxyl-9H- xanthen-9-one) was identified as the major bioactive component of MSBE. However, MSBE has shown more potent effects than model mangiferin solutions in several in vitro experiments at different concentrations. Therefore, there are other extract components which contribute to the observed pharmacological effects. Objective: The identification of other polyphenolic components in MSBE, which may contribute to the observed pharmacological effects through a synergic pathway. Method: Polyphenol-rich extracts from mango stem bark and branch trees from two varieties (Haden and Tommy Atkins), cultivated in Dominican Republic, were analyzed by HPLC-DAD. Butanol MSBE extract from Haden mango stem bark was analyzed by HPLC-MS-ESI. Results: The mangiferin content in Haden mango extracts by HPLC-DAD was considerably high as compared to previous reports in the literature from other mango varieties. The identification of Haden mango stem bark butanolic extract components by HPLC-ESI-MS led to the unambiguous identification of 20 components: 2 benzoic acid derivatives, gallic acid and 8 gallate derivatives, 2 benzophenones (maclaurin and iriflophenone types), 3 flavonoids (catechin, epicatechin, and quercetin), mangiferin, isomangiferin, homomangiferin, and noratyriol. Conclusion: HPLC-ESI-MS analysis of the Haden mango SB extract showed the presence of many polyphenolic components, not previously reported, which may correlate to the antioxidant, anti-inflammatory, and/or analgesic effects of this extract mostly through a synergistic effect of its components. The potential exploitation of mango by-products from the Haden variety would be the best option for obtaining polyphenol-rich extracts from mango agricultural by-products to be used as bioactive ingredients in nutraceutical, cosmeceutical, and/or pharmaceutical formulations.},
 year = {2020}
}

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TY  - JOUR
T1  - HPLC-DAD and HPLC-ESI-MS Analysis of Polyphenol-rich Extracts from Mango (Mangifera indica L.), Tommy Atkins and Haden Varieties, Cultivated in Dominican Republic
AU  - Alberto Julio Nunez-Selles
AU  - Victor Manuel Espaillat Martínez
AU  - Lauro Nuevas Paz
Y1  - 2020/12/16
PY  - 2020
N1  - https://doi.org/10.11648/j.ijpc.20200606.12
DO  - 10.11648/j.ijpc.20200606.12
T2  - International Journal of Pharmacy and Chemistry
JF  - International Journal of Pharmacy and Chemistry
JO  - International Journal of Pharmacy and Chemistry
SP  - 77
EP  - 88
PB  - Science Publishing Group
SN  - 2575-5749
UR  - https://doi.org/10.11648/j.ijpc.20200606.12
AB  - Background: Antioxidant, anti-inflammatory, and analgesic effects of mango stem bark extracts (MSBE) have been reported. A previous published report described the identification of several components (polyphenols, polyols, and sugars) by HPLC, MS, and NMR. Mangiferin (2-β-D-glucopyranosyl-1,3,6,7-tetrahydroxyl-9H- xanthen-9-one) was identified as the major bioactive component of MSBE. However, MSBE has shown more potent effects than model mangiferin solutions in several in vitro experiments at different concentrations. Therefore, there are other extract components which contribute to the observed pharmacological effects. Objective: The identification of other polyphenolic components in MSBE, which may contribute to the observed pharmacological effects through a synergic pathway. Method: Polyphenol-rich extracts from mango stem bark and branch trees from two varieties (Haden and Tommy Atkins), cultivated in Dominican Republic, were analyzed by HPLC-DAD. Butanol MSBE extract from Haden mango stem bark was analyzed by HPLC-MS-ESI. Results: The mangiferin content in Haden mango extracts by HPLC-DAD was considerably high as compared to previous reports in the literature from other mango varieties. The identification of Haden mango stem bark butanolic extract components by HPLC-ESI-MS led to the unambiguous identification of 20 components: 2 benzoic acid derivatives, gallic acid and 8 gallate derivatives, 2 benzophenones (maclaurin and iriflophenone types), 3 flavonoids (catechin, epicatechin, and quercetin), mangiferin, isomangiferin, homomangiferin, and noratyriol. Conclusion: HPLC-ESI-MS analysis of the Haden mango SB extract showed the presence of many polyphenolic components, not previously reported, which may correlate to the antioxidant, anti-inflammatory, and/or analgesic effects of this extract mostly through a synergistic effect of its components. The potential exploitation of mango by-products from the Haden variety would be the best option for obtaining polyphenol-rich extracts from mango agricultural by-products to be used as bioactive ingredients in nutraceutical, cosmeceutical, and/or pharmaceutical formulations.
VL  - 6
IS  - 6
ER  -

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