Effect of Ethanol Extract of <i>Moringa oleifera</i> Leaf on Nissl Granules, Immunohistochemistry of Inferior Colliculus and Oxidative System Following Quinine Toxicity in Wistar Rats

Idorenyin Umoh; Theresa Ekanem; Herbert Mbagwu; Justina Udotong

doi:doi:10.11648/j.ijcda.20210701.12

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Effect of Ethanol Extract of Moringa oleifera Leaf on Nissl Granules, Immunohistochemistry of Inferior Colliculus and Oxidative System Following Quinine Toxicity in Wistar Rats

Idorenyin Umoh, Theresa Ekanem, Herbert Mbagwu, Justina Udotong

Published in International Journal of Clinical and Developmental Anatomy (Volume 7, Issue 1)

Received: 30 September 2020 Accepted: 22 October 2020 Published: 10 March 2021

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Abstract

The metabolite of Quinine, 3-hydroxyquinine is the active substance and more toxic than quinine hence, it can exert pro-oxidant effect in the body. In this study, the effect of ethanol leaf extract of Moringa oleifera was evaluated against quinine toxicity on nissl granules, immunohistochemistry of inferior colliculus and oxidative system of Wistar rats. Fifty Wistar rats weighing 180 – 200g were alloted into 10 groups of 5 rats each. Group 1 served as control while Groups 2 to 10 were the treatment groups. Groups 2 – 4 received 10, 20 and 30 mg/kg body weight of quinine hypochloride injection intramuscularly eight hourly for 7 days. Groups 5 – 7 were orally administered 250, 500 and 750 mg/kg body weight of M. oleifera leaf extract daily for 7 days respectively. Group 8 received 250mg/kg of leaf extract orally and intramuscular (IM) 10mg/kg of quinine; Group 9 was treated with oral 500mg/kg of the extract and IM 20mg/kg of quinine while Group 10 received 750mg/kg of the extract orally and IM 30mg/kg of quinine for 7 days. Results revealed that quinine significantly (p < 0.05) decreased the activities of anti-oxidative enzymes (superoxide dismutase and catalase) and increased lipid peroxidation (malondialdehyde, MDA) in treated rats. However, Moringa oleifera leaf extract showed significant increase in the activities of anti-oxidant enzymes and decreased lipid peroxidation. Histomorphological and immunohistochemical observations revealed that quinine caused neuronal distortions, nuclear degenerations, and depletion of Nissl granules, vascular congestions and vacuolations and increased astrogliosis in the inferior colliculus. Incorporation of the extract to the quinine treated groups revealed neuronal regenerations, increased staining intensity and density of the Nissl granules, reduced astrogliosis and restoration in the inferior colliculuscyto-architectures. In conclusion, Moringa oleifera leaf extract can be used to ameliorate the adverse effects of quinine toxicity on inferior colliculus.

Published in	International Journal of Clinical and Developmental Anatomy (Volume 7, Issue 1)
DOI	10.11648/j.ijcda.20210701.12
Page(s)	6-17
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Quinine, Moringa oleifera, Inferior Colliculus, Nissl Granules, Oxidative Enzymes

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Idorenyin Umoh, Theresa Ekanem, Herbert Mbagwu, Justina Udotong. (2021). Effect of Ethanol Extract of Moringa oleifera Leaf on Nissl Granules, Immunohistochemistry of Inferior Colliculus and Oxidative System Following Quinine Toxicity in Wistar Rats. International Journal of Clinical and Developmental Anatomy, 7(1), 6-17. https://doi.org/10.11648/j.ijcda.20210701.12

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ACS Style

Idorenyin Umoh; Theresa Ekanem; Herbert Mbagwu; Justina Udotong. Effect of Ethanol Extract of Moringa oleifera Leaf on Nissl Granules, Immunohistochemistry of Inferior Colliculus and Oxidative System Following Quinine Toxicity in Wistar Rats. Int. J. Clin. Dev. Anat. 2021, 7(1), 6-17. doi: 10.11648/j.ijcda.20210701.12

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AMA Style

Idorenyin Umoh, Theresa Ekanem, Herbert Mbagwu, Justina Udotong. Effect of Ethanol Extract of Moringa oleifera Leaf on Nissl Granules, Immunohistochemistry of Inferior Colliculus and Oxidative System Following Quinine Toxicity in Wistar Rats. Int J Clin Dev Anat. 2021;7(1):6-17. doi: 10.11648/j.ijcda.20210701.12

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@article{10.11648/j.ijcda.20210701.12,
  author = {Idorenyin Umoh and Theresa Ekanem and Herbert Mbagwu and Justina Udotong},
  title = {Effect of Ethanol Extract of Moringa oleifera Leaf on Nissl Granules, Immunohistochemistry of Inferior Colliculus and Oxidative System Following Quinine Toxicity in Wistar Rats},
  journal = {International Journal of Clinical and Developmental Anatomy},
  volume = {7},
  number = {1},
  pages = {6-17},
  doi = {10.11648/j.ijcda.20210701.12},
  url = {https://doi.org/10.11648/j.ijcda.20210701.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcda.20210701.12},
  abstract = {The metabolite of Quinine, 3-hydroxyquinine is the active substance and more toxic than quinine hence, it can exert pro-oxidant effect in the body. In this study, the effect of ethanol leaf extract of Moringa oleifera was evaluated against quinine toxicity on nissl granules, immunohistochemistry of inferior colliculus and oxidative system of Wistar rats. Fifty Wistar rats weighing 180 – 200g were alloted into 10 groups of 5 rats each. Group 1 served as control while Groups 2 to 10 were the treatment groups. Groups 2 – 4 received 10, 20 and 30 mg/kg body weight of quinine hypochloride injection intramuscularly eight hourly for 7 days. Groups 5 – 7 were orally administered 250, 500 and 750 mg/kg body weight of M. oleifera leaf extract daily for 7 days respectively. Group 8 received 250mg/kg of leaf extract orally and intramuscular (IM) 10mg/kg of quinine; Group 9 was treated with oral 500mg/kg of the extract and IM 20mg/kg of quinine while Group 10 received 750mg/kg of the extract orally and IM 30mg/kg of quinine for 7 days. Results revealed that quinine significantly (p Moringa oleifera leaf extract showed significant increase in the activities of anti-oxidant enzymes and decreased lipid peroxidation. Histomorphological and immunohistochemical observations revealed that quinine caused neuronal distortions, nuclear degenerations, and depletion of Nissl granules, vascular congestions and vacuolations and increased astrogliosis in the inferior colliculus. Incorporation of the extract to the quinine treated groups revealed neuronal regenerations, increased staining intensity and density of the Nissl granules, reduced astrogliosis and restoration in the inferior colliculuscyto-architectures. In conclusion, Moringa oleifera leaf extract can be used to ameliorate the adverse effects of quinine toxicity on inferior colliculus.},
 year = {2021}
}

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TY  - JOUR
T1  - Effect of Ethanol Extract of Moringa oleifera Leaf on Nissl Granules, Immunohistochemistry of Inferior Colliculus and Oxidative System Following Quinine Toxicity in Wistar Rats
AU  - Idorenyin Umoh
AU  - Theresa Ekanem
AU  - Herbert Mbagwu
AU  - Justina Udotong
Y1  - 2021/03/10
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcda.20210701.12
DO  - 10.11648/j.ijcda.20210701.12
T2  - International Journal of Clinical and Developmental Anatomy
JF  - International Journal of Clinical and Developmental Anatomy
JO  - International Journal of Clinical and Developmental Anatomy
SP  - 6
EP  - 17
PB  - Science Publishing Group
SN  - 2469-8008
UR  - https://doi.org/10.11648/j.ijcda.20210701.12
AB  - The metabolite of Quinine, 3-hydroxyquinine is the active substance and more toxic than quinine hence, it can exert pro-oxidant effect in the body. In this study, the effect of ethanol leaf extract of Moringa oleifera was evaluated against quinine toxicity on nissl granules, immunohistochemistry of inferior colliculus and oxidative system of Wistar rats. Fifty Wistar rats weighing 180 – 200g were alloted into 10 groups of 5 rats each. Group 1 served as control while Groups 2 to 10 were the treatment groups. Groups 2 – 4 received 10, 20 and 30 mg/kg body weight of quinine hypochloride injection intramuscularly eight hourly for 7 days. Groups 5 – 7 were orally administered 250, 500 and 750 mg/kg body weight of M. oleifera leaf extract daily for 7 days respectively. Group 8 received 250mg/kg of leaf extract orally and intramuscular (IM) 10mg/kg of quinine; Group 9 was treated with oral 500mg/kg of the extract and IM 20mg/kg of quinine while Group 10 received 750mg/kg of the extract orally and IM 30mg/kg of quinine for 7 days. Results revealed that quinine significantly (p Moringa oleifera leaf extract showed significant increase in the activities of anti-oxidant enzymes and decreased lipid peroxidation. Histomorphological and immunohistochemical observations revealed that quinine caused neuronal distortions, nuclear degenerations, and depletion of Nissl granules, vascular congestions and vacuolations and increased astrogliosis in the inferior colliculus. Incorporation of the extract to the quinine treated groups revealed neuronal regenerations, increased staining intensity and density of the Nissl granules, reduced astrogliosis and restoration in the inferior colliculuscyto-architectures. In conclusion, Moringa oleifera leaf extract can be used to ameliorate the adverse effects of quinine toxicity on inferior colliculus.
VL  - 7
IS  - 1
ER  -

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Author Information

Idorenyin Umoh

Department of Anatomy, Faculty of Basic Medical Sciences, University of Uyo, Uyo, Nigeria
Theresa Ekanem

Department of Anatomy, Faculty of Basic Medical Sciences, University of Uyo, Uyo, Nigeria
Herbert Mbagwu

Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria
Justina Udotong

Department of Biochemistry, Faculty of Basic Medical Sciences, University of Uyo, Uyo, Nigeria

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Idorenyin Umoh, Theresa Ekanem, Herbert Mbagwu, Justina Udotong. (2021). Effect of Ethanol Extract of Moringa oleifera Leaf on Nissl Granules, Immunohistochemistry of Inferior Colliculus and Oxidative System Following Quinine Toxicity in Wistar Rats. International Journal of Clinical and Developmental Anatomy, 7(1), 6-17. https://doi.org/10.11648/j.ijcda.20210701.12

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ACS Style

Idorenyin Umoh; Theresa Ekanem; Herbert Mbagwu; Justina Udotong. Effect of Ethanol Extract of Moringa oleifera Leaf on Nissl Granules, Immunohistochemistry of Inferior Colliculus and Oxidative System Following Quinine Toxicity in Wistar Rats. Int. J. Clin. Dev. Anat. 2021, 7(1), 6-17. doi: 10.11648/j.ijcda.20210701.12

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AMA Style

Idorenyin Umoh, Theresa Ekanem, Herbert Mbagwu, Justina Udotong. Effect of Ethanol Extract of Moringa oleifera Leaf on Nissl Granules, Immunohistochemistry of Inferior Colliculus and Oxidative System Following Quinine Toxicity in Wistar Rats. Int J Clin Dev Anat. 2021;7(1):6-17. doi: 10.11648/j.ijcda.20210701.12

Copy | Download

@article{10.11648/j.ijcda.20210701.12,
  author = {Idorenyin Umoh and Theresa Ekanem and Herbert Mbagwu and Justina Udotong},
  title = {Effect of Ethanol Extract of Moringa oleifera Leaf on Nissl Granules, Immunohistochemistry of Inferior Colliculus and Oxidative System Following Quinine Toxicity in Wistar Rats},
  journal = {International Journal of Clinical and Developmental Anatomy},
  volume = {7},
  number = {1},
  pages = {6-17},
  doi = {10.11648/j.ijcda.20210701.12},
  url = {https://doi.org/10.11648/j.ijcda.20210701.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcda.20210701.12},
  abstract = {The metabolite of Quinine, 3-hydroxyquinine is the active substance and more toxic than quinine hence, it can exert pro-oxidant effect in the body. In this study, the effect of ethanol leaf extract of Moringa oleifera was evaluated against quinine toxicity on nissl granules, immunohistochemistry of inferior colliculus and oxidative system of Wistar rats. Fifty Wistar rats weighing 180 – 200g were alloted into 10 groups of 5 rats each. Group 1 served as control while Groups 2 to 10 were the treatment groups. Groups 2 – 4 received 10, 20 and 30 mg/kg body weight of quinine hypochloride injection intramuscularly eight hourly for 7 days. Groups 5 – 7 were orally administered 250, 500 and 750 mg/kg body weight of M. oleifera leaf extract daily for 7 days respectively. Group 8 received 250mg/kg of leaf extract orally and intramuscular (IM) 10mg/kg of quinine; Group 9 was treated with oral 500mg/kg of the extract and IM 20mg/kg of quinine while Group 10 received 750mg/kg of the extract orally and IM 30mg/kg of quinine for 7 days. Results revealed that quinine significantly (p Moringa oleifera leaf extract showed significant increase in the activities of anti-oxidant enzymes and decreased lipid peroxidation. Histomorphological and immunohistochemical observations revealed that quinine caused neuronal distortions, nuclear degenerations, and depletion of Nissl granules, vascular congestions and vacuolations and increased astrogliosis in the inferior colliculus. Incorporation of the extract to the quinine treated groups revealed neuronal regenerations, increased staining intensity and density of the Nissl granules, reduced astrogliosis and restoration in the inferior colliculuscyto-architectures. In conclusion, Moringa oleifera leaf extract can be used to ameliorate the adverse effects of quinine toxicity on inferior colliculus.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Effect of Ethanol Extract of Moringa oleifera Leaf on Nissl Granules, Immunohistochemistry of Inferior Colliculus and Oxidative System Following Quinine Toxicity in Wistar Rats
AU  - Idorenyin Umoh
AU  - Theresa Ekanem
AU  - Herbert Mbagwu
AU  - Justina Udotong
Y1  - 2021/03/10
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcda.20210701.12
DO  - 10.11648/j.ijcda.20210701.12
T2  - International Journal of Clinical and Developmental Anatomy
JF  - International Journal of Clinical and Developmental Anatomy
JO  - International Journal of Clinical and Developmental Anatomy
SP  - 6
EP  - 17
PB  - Science Publishing Group
SN  - 2469-8008
UR  - https://doi.org/10.11648/j.ijcda.20210701.12
AB  - The metabolite of Quinine, 3-hydroxyquinine is the active substance and more toxic than quinine hence, it can exert pro-oxidant effect in the body. In this study, the effect of ethanol leaf extract of Moringa oleifera was evaluated against quinine toxicity on nissl granules, immunohistochemistry of inferior colliculus and oxidative system of Wistar rats. Fifty Wistar rats weighing 180 – 200g were alloted into 10 groups of 5 rats each. Group 1 served as control while Groups 2 to 10 were the treatment groups. Groups 2 – 4 received 10, 20 and 30 mg/kg body weight of quinine hypochloride injection intramuscularly eight hourly for 7 days. Groups 5 – 7 were orally administered 250, 500 and 750 mg/kg body weight of M. oleifera leaf extract daily for 7 days respectively. Group 8 received 250mg/kg of leaf extract orally and intramuscular (IM) 10mg/kg of quinine; Group 9 was treated with oral 500mg/kg of the extract and IM 20mg/kg of quinine while Group 10 received 750mg/kg of the extract orally and IM 30mg/kg of quinine for 7 days. Results revealed that quinine significantly (p Moringa oleifera leaf extract showed significant increase in the activities of anti-oxidant enzymes and decreased lipid peroxidation. Histomorphological and immunohistochemical observations revealed that quinine caused neuronal distortions, nuclear degenerations, and depletion of Nissl granules, vascular congestions and vacuolations and increased astrogliosis in the inferior colliculus. Incorporation of the extract to the quinine treated groups revealed neuronal regenerations, increased staining intensity and density of the Nissl granules, reduced astrogliosis and restoration in the inferior colliculuscyto-architectures. In conclusion, Moringa oleifera leaf extract can be used to ameliorate the adverse effects of quinine toxicity on inferior colliculus.
VL  - 7
IS  - 1
ER  -

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