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Bioremediation Potentials of Hydrocarbonoclastic Bacteria Isolated from Petroleum Refinery Effluent

Received: 8 February 2016     Accepted: 17 May 2016     Published: 9 December 2016
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Abstract

Petroleum effluent is discharged into River Rido and this poses a threat to the communities that utilize the water downstream of River Rido, the ultimate recipient of the effluent. A total of five hydrocarbonoclastic bacteria were isolated from the petroleum refinery effluent. The isolates were inoculated separately into mineral salt medium supplemented with 1% crude oil to determine their growth rate in a hydrocarbon substrate. All five bacterial isolates manifested no lag phase of growth. Proteus vulgaris, Klebsiella pneumoniae, Serretia marcescens and Streptomyces sp. exhibited exponential, stationary and death phases of growth while Pseudomonas aeruginosa had only exponential and stationary phases of growth on the 16th day of incubation. Mixed culture consortium of all five bacteria isolates had the highest bioremediation potential of 68% as against the single inoculations of Pseudomonas aeruginosa, Serratia marcescens, Klebsiella pneumoniae, Proteus vulgaris and Streptomyces spp. which had 66.4%, 62.4%, 59.7%, 52% and 57% respectively. Hence, the isolated bacteria can be used for bioremediation of petroleum effluent.

Published in Frontiers in Environmental Microbiology (Volume 2, Issue 6)
DOI 10.11648/j.fem.20160206.12
Page(s) 34-37
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Bioremediation, Hydrocarbonoclastic, Bacteria, Refinery Effluent

References
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[2] Bako S. P., Chukwunonsu D. and Adamu A. K. (2008). Bioremediation of Refinery Effluent By Strains Of Pseudomonas aeruginosa and Penicillium janthinellum. Applied Ecology and Environmental Research, 6(3): 49-60.
[3] Hamza U. D., Mohammed A. I. and Ibrahim S. (2009). Kinetics Of Biological Reduction Of Chemical Oxygen Demand From Petroleum Refinery Wastewater. Researcher, Vol 1(2).
[4] Holt J. G., Krieg N. R., Sneath P. H. A., Stanley J. T and Williams S. T (1994). “Bergey’s manual of determinative Bacteriology”. 9th Ed. Williams and Wilkins, Pp: 71-561.
[5] Jyothi K., Surendra K. B., Nancy C. K. and Kashyap A. (2012). Identification and Isolation of Hydrocarbon Degrading Bacteria By Molecular Characterization. Helix, 2: 105-111.
[6] Kaplan C. W and Kitts C. L (2004). “Bacterial succession in a petroleum land treatment unit”. Applied Environmental Microbiology, 70: 1777-1786.
[7] Leahy G. J and Rita R C. (1990). “Microbial Degradation of Hydrocarbons in the Environment”. Microbiology Revision, Pp: 305-315.s
[8] Ojumu T. V., Bello O., Sonibare J. A., and Solomon B. O. (2004). Evaluation Of Microbial Systems For Bioremediation Of Petroleum Refinery Effluents In Nigeria. African Journal Of Biotechnology, 4(1): 31-35.
[9] Ornston I. N. and Yeh W. K. (2010). “Recurring Themes And Repeated Sequences In Metabolic Evolution In Biodegradation and Detoxification Of Environmental Pollutants”. A. M hakrabart, (ed.), CRC press Miami.
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[11] Thenmozhi R., Nagasathya A. and Thajuddin N. (2011). Studies on Biodegradation of Used Engine Oil by Consortium Cultures. Advances in Environmental Biology, 5(6): 1051-1057.
[12] Usman D. H., Ibrahim A. M. and Abdullahi S. (2012). Potentials Of Bacterial Isolates In Bioremediation Of Petroleum Refinery Wastewater. Journal of Applied Phytotechnology in Environmental Sanitation, 1(3): 131-138.
[13] Van Hamme J. D., Singh A, Ward O. P. (2003). “Recent advances in petroleum microbiology”. Microbiology and Molecular Biology Reviews, 67:503-549.
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  • APA Style

    Enimie Endurance Oaikhena, Dominic Bawa Makaije, Samuel Denwe, Muhammad Muktar Namadi, Omolola Elizabeth Fatanmi. (2016). Bioremediation Potentials of Hydrocarbonoclastic Bacteria Isolated from Petroleum Refinery Effluent. Frontiers in Environmental Microbiology, 2(6), 34-37. https://doi.org/10.11648/j.fem.20160206.12

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    ACS Style

    Enimie Endurance Oaikhena; Dominic Bawa Makaije; Samuel Denwe; Muhammad Muktar Namadi; Omolola Elizabeth Fatanmi. Bioremediation Potentials of Hydrocarbonoclastic Bacteria Isolated from Petroleum Refinery Effluent. Front. Environ. Microbiol. 2016, 2(6), 34-37. doi: 10.11648/j.fem.20160206.12

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    AMA Style

    Enimie Endurance Oaikhena, Dominic Bawa Makaije, Samuel Denwe, Muhammad Muktar Namadi, Omolola Elizabeth Fatanmi. Bioremediation Potentials of Hydrocarbonoclastic Bacteria Isolated from Petroleum Refinery Effluent. Front Environ Microbiol. 2016;2(6):34-37. doi: 10.11648/j.fem.20160206.12

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  • @article{10.11648/j.fem.20160206.12,
      author = {Enimie Endurance Oaikhena and Dominic Bawa Makaije and Samuel Denwe and Muhammad Muktar Namadi and Omolola Elizabeth Fatanmi},
      title = {Bioremediation Potentials of Hydrocarbonoclastic Bacteria Isolated from Petroleum Refinery Effluent},
      journal = {Frontiers in Environmental Microbiology},
      volume = {2},
      number = {6},
      pages = {34-37},
      doi = {10.11648/j.fem.20160206.12},
      url = {https://doi.org/10.11648/j.fem.20160206.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20160206.12},
      abstract = {Petroleum effluent is discharged into River Rido and this poses a threat to the communities that utilize the water downstream of River Rido, the ultimate recipient of the effluent. A total of five hydrocarbonoclastic bacteria were isolated from the petroleum refinery effluent. The isolates were inoculated separately into mineral salt medium supplemented with 1% crude oil to determine their growth rate in a hydrocarbon substrate. All five bacterial isolates manifested no lag phase of growth. Proteus vulgaris, Klebsiella pneumoniae, Serretia marcescens and Streptomyces sp. exhibited exponential, stationary and death phases of growth while Pseudomonas aeruginosa had only exponential and stationary phases of growth on the 16th day of incubation. Mixed culture consortium of all five bacteria isolates had the highest bioremediation potential of 68% as against the single inoculations of Pseudomonas aeruginosa, Serratia marcescens, Klebsiella pneumoniae, Proteus vulgaris and Streptomyces spp. which had 66.4%, 62.4%, 59.7%, 52% and 57% respectively. Hence, the isolated bacteria can be used for bioremediation of petroleum effluent.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Bioremediation Potentials of Hydrocarbonoclastic Bacteria Isolated from Petroleum Refinery Effluent
    AU  - Enimie Endurance Oaikhena
    AU  - Dominic Bawa Makaije
    AU  - Samuel Denwe
    AU  - Muhammad Muktar Namadi
    AU  - Omolola Elizabeth Fatanmi
    Y1  - 2016/12/09
    PY  - 2016
    N1  - https://doi.org/10.11648/j.fem.20160206.12
    DO  - 10.11648/j.fem.20160206.12
    T2  - Frontiers in Environmental Microbiology
    JF  - Frontiers in Environmental Microbiology
    JO  - Frontiers in Environmental Microbiology
    SP  - 34
    EP  - 37
    PB  - Science Publishing Group
    SN  - 2469-8067
    UR  - https://doi.org/10.11648/j.fem.20160206.12
    AB  - Petroleum effluent is discharged into River Rido and this poses a threat to the communities that utilize the water downstream of River Rido, the ultimate recipient of the effluent. A total of five hydrocarbonoclastic bacteria were isolated from the petroleum refinery effluent. The isolates were inoculated separately into mineral salt medium supplemented with 1% crude oil to determine their growth rate in a hydrocarbon substrate. All five bacterial isolates manifested no lag phase of growth. Proteus vulgaris, Klebsiella pneumoniae, Serretia marcescens and Streptomyces sp. exhibited exponential, stationary and death phases of growth while Pseudomonas aeruginosa had only exponential and stationary phases of growth on the 16th day of incubation. Mixed culture consortium of all five bacteria isolates had the highest bioremediation potential of 68% as against the single inoculations of Pseudomonas aeruginosa, Serratia marcescens, Klebsiella pneumoniae, Proteus vulgaris and Streptomyces spp. which had 66.4%, 62.4%, 59.7%, 52% and 57% respectively. Hence, the isolated bacteria can be used for bioremediation of petroleum effluent.
    VL  - 2
    IS  - 6
    ER  - 

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Author Information
  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria

  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria

  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria

  • Department of Chemistry, Nigerian Defence Academy, Kaduna, Nigeria

  • Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria

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