Characterization of Bacterial Populations in Urban and Rural Houston Watershed Soil Samples Following a Flooding Event

Folasade Tinuke Adedoyin; Maruthi Sridhar Balaji Bhaskar; Jason Alan Rosenzweig

doi:doi:10.11648/j.fem.20210701.14

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Characterization of Bacterial Populations in Urban and Rural Houston Watershed Soil Samples Following a Flooding Event

Folasade Tinuke Adedoyin, Maruthi Sridhar Balaji Bhaskar, Jason Alan Rosenzweig

Published in Frontiers in Environmental Microbiology (Volume 7, Issue 1)

Received: 11 February 2021 Accepted: 24 February 2021 Published: 12 March 2021

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Abstract

Since Houston is prone to flooding events, bacterial population dynamics in Houston watershed soils pre- and post-Hurricane Harvey were evaluated. Unexpectedly, bayous closer to Houston’s urban core, including Buffalo, Halls, Mustang, and Horsepen Bayous, had significantly higher enteric bacterial loads during the winter than the summer, likely due to water flow rate changes or proximity to wastewater outflow. Following bacterial load determination, isolated colonies were identified using biochemical tests. Additionally, metagenomic sequencing of 16S rDNA allowed for identification of both culturable and unculturable organisms. The phyla Proteobacteria, Actinobacteria, Bacteriodetes and Firmicutes were found to be dominant in our metagenomic analysis and are human gut bacteria. Some opportunistic bacterial Proteobacteria pathogens identified in our metabolomic analysis were Serratia marcenscens, Pseudomonas mendocina, Pseudomonas fulva, and Pseudomonas putida. To our knowledge, this is the first study that compares Houston-area bacterial populations before and after a major flooding event. Taken together, Hurricane Harvey likely contributed to a redistribution of enteric bacteria, as there was a significant increase in the enteric population of Buffalo and Halls Bayous. Similarly, our 2018 winter data set followed the same trend, as significant increases were seen in the enteric populations of Horsepen, Mustang, and Cypress Creek watershed soils.

Published in	Frontiers in Environmental Microbiology (Volume 7, Issue 1)
DOI	10.11648/j.fem.20210701.14
Page(s)	22-34
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Enteric Bacteria, Environmental Isolates, Biochemical Analysis, Metagenomic, GIS

References

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Folasade Tinuke Adedoyin, Maruthi Sridhar Balaji Bhaskar, Jason Alan Rosenzweig. (2021). Characterization of Bacterial Populations in Urban and Rural Houston Watershed Soil Samples Following a Flooding Event. Frontiers in Environmental Microbiology, 7(1), 22-34. https://doi.org/10.11648/j.fem.20210701.14

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Folasade Tinuke Adedoyin; Maruthi Sridhar Balaji Bhaskar; Jason Alan Rosenzweig. Characterization of Bacterial Populations in Urban and Rural Houston Watershed Soil Samples Following a Flooding Event. Front. Environ. Microbiol. 2021, 7(1), 22-34. doi: 10.11648/j.fem.20210701.14

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AMA Style

Folasade Tinuke Adedoyin, Maruthi Sridhar Balaji Bhaskar, Jason Alan Rosenzweig. Characterization of Bacterial Populations in Urban and Rural Houston Watershed Soil Samples Following a Flooding Event. Front Environ Microbiol. 2021;7(1):22-34. doi: 10.11648/j.fem.20210701.14

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@article{10.11648/j.fem.20210701.14,
  author = {Folasade Tinuke Adedoyin and Maruthi Sridhar Balaji Bhaskar and Jason Alan Rosenzweig},
  title = {Characterization of Bacterial Populations in Urban and Rural Houston Watershed Soil Samples Following a Flooding Event},
  journal = {Frontiers in Environmental Microbiology},
  volume = {7},
  number = {1},
  pages = {22-34},
  doi = {10.11648/j.fem.20210701.14},
  url = {https://doi.org/10.11648/j.fem.20210701.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20210701.14},
  abstract = {Since Houston is prone to flooding events, bacterial population dynamics in Houston watershed soils pre- and post-Hurricane Harvey were evaluated. Unexpectedly, bayous closer to Houston’s urban core, including Buffalo, Halls, Mustang, and Horsepen Bayous, had significantly higher enteric bacterial loads during the winter than the summer, likely due to water flow rate changes or proximity to wastewater outflow. Following bacterial load determination, isolated colonies were identified using biochemical tests. Additionally, metagenomic sequencing of 16S rDNA allowed for identification of both culturable and unculturable organisms. The phyla Proteobacteria, Actinobacteria, Bacteriodetes and Firmicutes were found to be dominant in our metagenomic analysis and are human gut bacteria. Some opportunistic bacterial Proteobacteria pathogens identified in our metabolomic analysis were Serratia marcenscens, Pseudomonas mendocina, Pseudomonas fulva, and Pseudomonas putida. To our knowledge, this is the first study that compares Houston-area bacterial populations before and after a major flooding event. Taken together, Hurricane Harvey likely contributed to a redistribution of enteric bacteria, as there was a significant increase in the enteric population of Buffalo and Halls Bayous. Similarly, our 2018 winter data set followed the same trend, as significant increases were seen in the enteric populations of Horsepen, Mustang, and Cypress Creek watershed soils.},
 year = {2021}
}

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TY  - JOUR
T1  - Characterization of Bacterial Populations in Urban and Rural Houston Watershed Soil Samples Following a Flooding Event
AU  - Folasade Tinuke Adedoyin
AU  - Maruthi Sridhar Balaji Bhaskar
AU  - Jason Alan Rosenzweig
Y1  - 2021/03/12
PY  - 2021
N1  - https://doi.org/10.11648/j.fem.20210701.14
DO  - 10.11648/j.fem.20210701.14
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 22
EP  - 34
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20210701.14
AB  - Since Houston is prone to flooding events, bacterial population dynamics in Houston watershed soils pre- and post-Hurricane Harvey were evaluated. Unexpectedly, bayous closer to Houston’s urban core, including Buffalo, Halls, Mustang, and Horsepen Bayous, had significantly higher enteric bacterial loads during the winter than the summer, likely due to water flow rate changes or proximity to wastewater outflow. Following bacterial load determination, isolated colonies were identified using biochemical tests. Additionally, metagenomic sequencing of 16S rDNA allowed for identification of both culturable and unculturable organisms. The phyla Proteobacteria, Actinobacteria, Bacteriodetes and Firmicutes were found to be dominant in our metagenomic analysis and are human gut bacteria. Some opportunistic bacterial Proteobacteria pathogens identified in our metabolomic analysis were Serratia marcenscens, Pseudomonas mendocina, Pseudomonas fulva, and Pseudomonas putida. To our knowledge, this is the first study that compares Houston-area bacterial populations before and after a major flooding event. Taken together, Hurricane Harvey likely contributed to a redistribution of enteric bacteria, as there was a significant increase in the enteric population of Buffalo and Halls Bayous. Similarly, our 2018 winter data set followed the same trend, as significant increases were seen in the enteric populations of Horsepen, Mustang, and Cypress Creek watershed soils.
VL  - 7
IS  - 1
ER  -

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Author Information

Folasade Tinuke Adedoyin

Department of Environmental and Interdisciplinary Science, Texas Southern University, Houston, the United States
Maruthi Sridhar Balaji Bhaskar

Department of Earth and Environment Florida International University, Miami, the United States
Jason Alan Rosenzweig

Department of Biology Texas Southern University, Houston, the United States

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APA Style

Folasade Tinuke Adedoyin, Maruthi Sridhar Balaji Bhaskar, Jason Alan Rosenzweig. (2021). Characterization of Bacterial Populations in Urban and Rural Houston Watershed Soil Samples Following a Flooding Event. Frontiers in Environmental Microbiology, 7(1), 22-34. https://doi.org/10.11648/j.fem.20210701.14

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ACS Style

Folasade Tinuke Adedoyin; Maruthi Sridhar Balaji Bhaskar; Jason Alan Rosenzweig. Characterization of Bacterial Populations in Urban and Rural Houston Watershed Soil Samples Following a Flooding Event. Front. Environ. Microbiol. 2021, 7(1), 22-34. doi: 10.11648/j.fem.20210701.14

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AMA Style

Folasade Tinuke Adedoyin, Maruthi Sridhar Balaji Bhaskar, Jason Alan Rosenzweig. Characterization of Bacterial Populations in Urban and Rural Houston Watershed Soil Samples Following a Flooding Event. Front Environ Microbiol. 2021;7(1):22-34. doi: 10.11648/j.fem.20210701.14

Copy | Download

@article{10.11648/j.fem.20210701.14,
  author = {Folasade Tinuke Adedoyin and Maruthi Sridhar Balaji Bhaskar and Jason Alan Rosenzweig},
  title = {Characterization of Bacterial Populations in Urban and Rural Houston Watershed Soil Samples Following a Flooding Event},
  journal = {Frontiers in Environmental Microbiology},
  volume = {7},
  number = {1},
  pages = {22-34},
  doi = {10.11648/j.fem.20210701.14},
  url = {https://doi.org/10.11648/j.fem.20210701.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20210701.14},
  abstract = {Since Houston is prone to flooding events, bacterial population dynamics in Houston watershed soils pre- and post-Hurricane Harvey were evaluated. Unexpectedly, bayous closer to Houston’s urban core, including Buffalo, Halls, Mustang, and Horsepen Bayous, had significantly higher enteric bacterial loads during the winter than the summer, likely due to water flow rate changes or proximity to wastewater outflow. Following bacterial load determination, isolated colonies were identified using biochemical tests. Additionally, metagenomic sequencing of 16S rDNA allowed for identification of both culturable and unculturable organisms. The phyla Proteobacteria, Actinobacteria, Bacteriodetes and Firmicutes were found to be dominant in our metagenomic analysis and are human gut bacteria. Some opportunistic bacterial Proteobacteria pathogens identified in our metabolomic analysis were Serratia marcenscens, Pseudomonas mendocina, Pseudomonas fulva, and Pseudomonas putida. To our knowledge, this is the first study that compares Houston-area bacterial populations before and after a major flooding event. Taken together, Hurricane Harvey likely contributed to a redistribution of enteric bacteria, as there was a significant increase in the enteric population of Buffalo and Halls Bayous. Similarly, our 2018 winter data set followed the same trend, as significant increases were seen in the enteric populations of Horsepen, Mustang, and Cypress Creek watershed soils.},
 year = {2021}
}

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TY  - JOUR
T1  - Characterization of Bacterial Populations in Urban and Rural Houston Watershed Soil Samples Following a Flooding Event
AU  - Folasade Tinuke Adedoyin
AU  - Maruthi Sridhar Balaji Bhaskar
AU  - Jason Alan Rosenzweig
Y1  - 2021/03/12
PY  - 2021
N1  - https://doi.org/10.11648/j.fem.20210701.14
DO  - 10.11648/j.fem.20210701.14
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 22
EP  - 34
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20210701.14
AB  - Since Houston is prone to flooding events, bacterial population dynamics in Houston watershed soils pre- and post-Hurricane Harvey were evaluated. Unexpectedly, bayous closer to Houston’s urban core, including Buffalo, Halls, Mustang, and Horsepen Bayous, had significantly higher enteric bacterial loads during the winter than the summer, likely due to water flow rate changes or proximity to wastewater outflow. Following bacterial load determination, isolated colonies were identified using biochemical tests. Additionally, metagenomic sequencing of 16S rDNA allowed for identification of both culturable and unculturable organisms. The phyla Proteobacteria, Actinobacteria, Bacteriodetes and Firmicutes were found to be dominant in our metagenomic analysis and are human gut bacteria. Some opportunistic bacterial Proteobacteria pathogens identified in our metabolomic analysis were Serratia marcenscens, Pseudomonas mendocina, Pseudomonas fulva, and Pseudomonas putida. To our knowledge, this is the first study that compares Houston-area bacterial populations before and after a major flooding event. Taken together, Hurricane Harvey likely contributed to a redistribution of enteric bacteria, as there was a significant increase in the enteric population of Buffalo and Halls Bayous. Similarly, our 2018 winter data set followed the same trend, as significant increases were seen in the enteric populations of Horsepen, Mustang, and Cypress Creek watershed soils.
VL  - 7
IS  - 1
ER  -

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