Determination of the needed amount of liquid sterile Lactamator™ to be mixed to cephalexin, β-lactam antibiotic, for optimum deactivation of its molecule's antibacterial properties was conducted using RP-HPLC method. RP-HPLC method was validated for the parameters as linearity, accuracy, LOD, LOQ, and precision. Before the routine microbiological examination for any pharmaceutical dosage form containing β-lactam antibiotic, it is a must to make inactivate of β-lactam active pharmaceutical ingredient (API) by mixing with beta-lactamase before testing. So, the study indicated that mixing of 0.5 ml liquid sterile Lactamator™ with phosphate buffer solution pH (7.2) containing 50 mg cephalexin, with holding the test sample for 90 minutes prior to HPLC measurement will deactivation of cefalexin molecule's antibacterial properties.
| Published in | World Journal of Applied Chemistry (Volume 2, Issue 4) |
| DOI | 10.11648/j.wjac.20170204.11 |
| Page(s) | 120-128 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Cephalexin, Lactamator, Keflex, Method Validation
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APA Style
Mahmoud Faysal Mshref, Hesham Mohamed Ghonemy, Amr Helmy Ali. (2017). Determination of Beta-Lactamase Inactivation of Cephalexin by Validated RP-HPLC Method. World Journal of Applied Chemistry, 2(4), 120-128. https://doi.org/10.11648/j.wjac.20170204.11
ACS Style
Mahmoud Faysal Mshref; Hesham Mohamed Ghonemy; Amr Helmy Ali. Determination of Beta-Lactamase Inactivation of Cephalexin by Validated RP-HPLC Method. World J. Appl. Chem. 2017, 2(4), 120-128. doi: 10.11648/j.wjac.20170204.11
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Download@article{10.11648/j.wjac.20170204.11,
author = {Mahmoud Faysal Mshref and Hesham Mohamed Ghonemy and Amr Helmy Ali},
title = {Determination of Beta-Lactamase Inactivation of Cephalexin by Validated RP-HPLC Method},
journal = {World Journal of Applied Chemistry},
volume = {2},
number = {4},
pages = {120-128},
doi = {10.11648/j.wjac.20170204.11},
url = {https://doi.org/10.11648/j.wjac.20170204.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjac.20170204.11},
abstract = {Determination of the needed amount of liquid sterile Lactamator™ to be mixed to cephalexin, β-lactam antibiotic, for optimum deactivation of its molecule's antibacterial properties was conducted using RP-HPLC method. RP-HPLC method was validated for the parameters as linearity, accuracy, LOD, LOQ, and precision. Before the routine microbiological examination for any pharmaceutical dosage form containing β-lactam antibiotic, it is a must to make inactivate of β-lactam active pharmaceutical ingredient (API) by mixing with beta-lactamase before testing. So, the study indicated that mixing of 0.5 ml liquid sterile Lactamator™ with phosphate buffer solution pH (7.2) containing 50 mg cephalexin, with holding the test sample for 90 minutes prior to HPLC measurement will deactivation of cefalexin molecule's antibacterial properties.},
year = {2017}
}
TY - JOUR T1 - Determination of Beta-Lactamase Inactivation of Cephalexin by Validated RP-HPLC Method AU - Mahmoud Faysal Mshref AU - Hesham Mohamed Ghonemy AU - Amr Helmy Ali Y1 - 2017/09/13 PY - 2017 N1 - https://doi.org/10.11648/j.wjac.20170204.11 DO - 10.11648/j.wjac.20170204.11 T2 - World Journal of Applied Chemistry JF - World Journal of Applied Chemistry JO - World Journal of Applied Chemistry SP - 120 EP - 128 PB - Science Publishing Group SN - 2637-5982 UR - https://doi.org/10.11648/j.wjac.20170204.11 AB - Determination of the needed amount of liquid sterile Lactamator™ to be mixed to cephalexin, β-lactam antibiotic, for optimum deactivation of its molecule's antibacterial properties was conducted using RP-HPLC method. RP-HPLC method was validated for the parameters as linearity, accuracy, LOD, LOQ, and precision. Before the routine microbiological examination for any pharmaceutical dosage form containing β-lactam antibiotic, it is a must to make inactivate of β-lactam active pharmaceutical ingredient (API) by mixing with beta-lactamase before testing. So, the study indicated that mixing of 0.5 ml liquid sterile Lactamator™ with phosphate buffer solution pH (7.2) containing 50 mg cephalexin, with holding the test sample for 90 minutes prior to HPLC measurement will deactivation of cefalexin molecule's antibacterial properties. VL - 2 IS - 4 ER -
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