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Resistance of Bacteria Isolates from Cabbage (Brassica oleracea), Carrots (Daucus carota) and Lettuce (Lactuca sativa) in the Kumasi Metropolis of Ghana

Despite the benefits humanity derives from vegetables, they are as well a good source of foodborne illnesses when contaminated with pathogenic microorganisms especially the antibiotic resistant ones. This study evaluated the antibiotic resistance of bacteria isolated from three commonly consumed vegetables; cabbage (Brassica oleracea), carrots (Daucus carota) and lettuce (Lactuca sativa) in the Kumasi Metropolis in Ghana. The bacteria isolates were subjected to antimicrobial susceptibility testing using the Kirby-Bauer disc diffusion method on Mueller-Hinton agar. A total of 78 bacteria isolates were obtained from the three vegetables with the Gram-positive bacteria accounting for 23.08% (18/78) whilst the Gram-negative bacteria accounted for 76.92% (60/78). The Gram-negative bacteria consisted of Acinectobacter spp., Aeromonas salmonicida, Aeromonas spp., Chryseomonas loteola, Citrobacter spp., Enterobacter cloacae, Enterobacter sakazakii, Erwinia nigrifluence, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeroginosa, Salmonella spp. and Serratia spp. while Gram-positive bacteria consisted of Brevibacillus spp., Brochothrix thermosphacter, Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus spp. All the Gram-positive bacteria isolates showed 100% resistance to penicillin, ampicillin and flucloxacillin, 88.2% were resistant to cefuroxime with 64.7% resistant to tetracycline and 17.6% resistant to cotrimoxazole but were all sensitive to gentamycin. For the Gram-negative bacteria, 96.7% were resistant to ampicillin with 90% being resistant to cefuroxime, 73.3% and 60% were resistant to chloramphenicol and tetracycline, respectively, but unlike the Gram-positives, 15% of the Gram-negative bacteria were resistant to gentamycin. All the Gram-positive isolates demonstrated multi antibiotic resistance (MAR), with Staphylococcus aureus and Streptococcus spp. being resistant to seven (7) out of the eight (8) tested antibiotics. For the Gram-negative bacteria, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeroginosa, Salmonella spp., Citrobacter spp. and Enterobacter were resistant to all eight (8) antibiotics. The implication of the findings in this study presents vegetables as a potential vehicle for microbial food poisoning as well as a source of infectious diseases that cannot be treated with commonly used antibiotics. Thus, good practices should be undertaken to reduce if not eliminate contamination of vegetables.

Bacteria, Ghana, Multiple Antibiotic Resistance, Prevalence, Vegetables

APA Style

Golly Moses Kwaku, Salifu Pandam Samson, Mills-Robertson Felix Charles. (2016). Resistance of Bacteria Isolates from Cabbage (Brassica oleracea), Carrots (Daucus carota) and Lettuce (Lactuca sativa) in the Kumasi Metropolis of Ghana. International Journal of Nutrition and Food Sciences, 5(4), 297-303.

ACS Style

Golly Moses Kwaku; Salifu Pandam Samson; Mills-Robertson Felix Charles. Resistance of Bacteria Isolates from Cabbage (Brassica oleracea), Carrots (Daucus carota) and Lettuce (Lactuca sativa) in the Kumasi Metropolis of Ghana. Int. J. Nutr. Food Sci. 2016, 5(4), 297-303. doi: 10.11648/j.ijnfs.20160504.20

AMA Style

Golly Moses Kwaku, Salifu Pandam Samson, Mills-Robertson Felix Charles. Resistance of Bacteria Isolates from Cabbage (Brassica oleracea), Carrots (Daucus carota) and Lettuce (Lactuca sativa) in the Kumasi Metropolis of Ghana. Int J Nutr Food Sci. 2016;5(4):297-303. doi: 10.11648/j.ijnfs.20160504.20

Copyright © 2016 Authors retain the copyright of this article.
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1. P. Gilbert and A. J. McBain, “Potential impact of increased use of biocides in consumer products on prevalence of antibiotic resistance,” Clin. Microbiol. Rev., vol. 16 (2), pp. 189-208, April 2003.
2. P. Mensah, M. Armar-Klemesu, A. S. Hammond, A. Haruna, and R. Nyarku, “Bacteria contaminants of lettuce, tomatoes, beef and goat meat from the Accra Metropolis,” Ghana Med. J., vol. 35, pp. 1-6, May 2001.
3. A. S. Obeng, A. G. Kwakye-Nuako, R. H. Asmah, and P. F. Ayeh-Kumi, “Pathogenic parasitic microbes associated with vegetables consumed in Accra,” GJAHS, vol. 1, pp. 11-15, June 2007.
4. E. S. Donkor, B. B. Kayang, J. Quaye, D. A. Edoh, and G. Owusu-Okyere, “Microbial contaminants of green pepper sold in Accra,” GJAHS, vol. 2, pp. 22-28, September 2009.
5. N. M. Nipa, R. M. Mazumdar, M. Md. Hasan, Md. Fakruddin, S. Islam, et al., “Prevalence of multi drug resistant bacteria on raw salad vegetables sold in major markets of Chittagong city, Banglagesh,” Middle-East J. Sci. Res., vol. 10 (1) pp. 70-77, 2011.
6. R. R. Roberts, B. Hota, I. Ahmad, R. D. I. I. Scott, S. D. Foster, et at., “Hospital and societal costs of antimicrobial-resistant infections in a Chicago teaching hospital: implications for antibiotic stewardship,” Clin. Infect Dis., vol. 49, pp. 1175-1178, October 2009.
7. F. J. Angulo, V. N. Nargund, and T. C. Chiller, “Evidence of an association between use of anti‐microbial agents in food animals and anti‐microbial resistance among bacteria isolated from humans and the human health consequences of such resistance,” J. Vet. Med. B. Infect Dis. Vet. Public Health, vol. 51, pp. 374‐379, October-November 2004.
8. C. Walsh, and S. Fanning, “Antimicrobial resistance in foodborne pathogens-a cause for concern?” Curr. Drug Targets, vol. 9 (9), pp. 808-15. September 2008.
9. WHO, Antimicrobial resistance, global report on surveillance. WHO Press, Geneva, Switzerland, pg. 1-6, 2014.
10. O. M. David, and A. O. Oluyege, “Antibiotic resistance and plasmid carriage among Salmonella typhi isolated from food and hands of food handlers in Nigeria University,” Int. J. Curr. Microbiol. App. Sci. vol. 4 (3), pp. 906-914, August 2015.
11. WHO, Antimicrobial resistance. Fact Sheet N°194, Updated April, 2015. (accessed 20 June 2016).
12. S. T. Odonkor, and K. K. Addo, “Bacteria Resistance to Antibiotics: Recent Trends and Challenges,” Int. J. Biol. Med. Res., vol. 2 (4), pp. 1204-1210, October 2011.
13. A. D. Russell, “Whither triclosan.?,” J. Antimicrob. Chemother. vol. 53 pp. 693–5, April 2004.
14. P. Courvalin, “Antimicrobial drug resistance: prediction is very difficult, especially about the future,” Emerging Infectious Diseases, vol. 11 (10), pp. 1503-1506, October 2005.
15. W. Lingling, and Y. Zhongtang, “Antimicrobial resistance arising from food-animal productions and its mitigation, antibiotic resistant bacteria-a continuous challenge in the new millennium,” Dr. Marina Pana (Ed.), April 2012. ISBN: 978-953-51-0472-8.
16. APUA, (Alliance for the Prudent Use of Antibiotics), “Antibiotic resistance, a societal problem,” 2014 (accessed 23 May 2016).
17. WHO (2000): In M. K. Ibrahim, A. M. Gala, I. M. Al-Turk, and K. D. Al-Zhrany, “Antibiotic resistance in Gram-negative pathogenic bacteria in hospitals' drain in A-Madina Al-Munnawara,” JTUSCI, vol. 3, pp. 14-22, January 2010.
18. A. Erb, and T. Sturmer, “Prevalence of antibiotic resistance in Escherichia coli: overview of geographical, temporal, and methodological variations,” Eur. J. Clin. Microbiol. Infect. Dis., vol. 26 (2), pp. 83-90, February 2007.
19. R. Laxminarayan, “Extending the cure: policy responses to the growing threat of antibiotic resistance,” RFF (Resources for the Future), Washington, DC, 2007.
20. A. Mora, and J. E. Blanco, “Antimicrobial resistance of Shiga toxin (verotoxin)-producing Escherichia coli O157: H7 and non-O157 strains isolated from humans, cattle, sheep and food in Spain,” Res. Microbiol., vol. 156 (7), pp. 793-806, August 2005.
21. D. L. Stevens, and Y. Ma, “Impact of antibiotics on expression of virulence-associated exotoxin genes in methicillin-sensitive and methicillin-resistant Staphylococcus aureus,” J. Infect. Dis., vol. 195 (2), pp. 202-211, January 2007.
22. R. M. Klevens, M. A. Morrison, J. Nadle, S. Petit, K. Gershman, and S. Ray, et al., “Active Bacterial Core surveillance (ABCs) MRSA Investigators. Invasive methicillin-resistant Staphylococcus aureus infections in the United States,” JAMA, vol. 298 (15), pp. 1763-71, October 2007.
23. T. Luangtongkum, B. Jeon, J. Han, Plumme, P, C. M. Logue, and Q. Zhang, “Antibiotic resistance in Campylobacter: emergence, transmission and persistence,” Future Microbiol., vol. 4 (2), pp. 189-200, March 2009.
24. A. Ohene, “Bacterial pathogens and their antimicrobial susceptibility in Kumasi, Ghana,” East Afr. Med. J., vol. 74 (7), pp. 450–455, July 1997.
25. J. A. Opintan, and M. J. Newman, “Distribution of serogroups and serotypes of multiple drug resistant Shigella isolates,” Ghana Med. J., vol. 41 (1), pp. 50–54, March 2007.
26. M. J. Newman, E. Frimpong, E. S. Donkor, J. A. Opintan, and A. Asamoah-Adu, “Resistance to antimicrobial drugs in Ghana,” Infections and Drug Resistance, vol. 4, pp. 215-220, December 2011.
27. P. Amoah, B. Keraita, M. Akple, P. Drechsel, R. C. Abaidoo, and F. Konradsen, “Low-cost options for reducing consumer health risks from farm to fork where crops are irrigated with polluted water in West Africa. IWMI Research Report 141, 2011. Colombo: International Water Management Institute. doi: 10.5337/2011.201.
28. F. Amponsah-Doku, K. Obiri-Danso, R. C. Abaidoo, L. A. Andoh, P. Drechesel, and F. Kondrasen, “Bacterial contamination of lettuce and associated risk factors at production sites, markets and street food restaurants in urban and peri-urban Kumasi, Ghana,” Scientific Research and Essay, vol. 5 (2), pp. 217-223, January 2010.
29. A. Canizalez-Roman, E. Gonzalez-Nunez, J. E. Vidal, H. Flores-Villasenor, N and Leon-Sicairos, “Prevalence and antibiotic resistance profiles of diarrheagenic Escherichia coli strains isolated from food items in northwestern Mexico,” Int. J. Food Microbiol., vol. 164, pp. 36–45, March 2013. (doi: 10.1016/j.ijfoodmicro.2013.03.020).
30. CLSI (Clinical and Laboratory Standards Institute), “Performance standards for antimicrobial susceptibility testing,” in Twenty-First Informational Suplement., Vol. 31, 2011, (Wayne, PA: Clinical and Laboratory Standards Institute), M02-A10 and M07-A08.
31. W. Chihab, A. S. Alaoui, and M. Amar, “Chryseomonas luteola Identified as the Source of Serious Infections in a Moroccan University Hospital,” Journal of Clinical Microbiology, vol. 42 (4), pp. 1837-1839, April 2004. doi: 10.1128/JCM.42.4.1837-1839.
32. A. Davin-Regli, and J-M. Pagès, “Enterobacter aerogenes and Enterobacter cloacae; versatile bacterial pathogens confronting antibiotic treatment,” Frontiers in Microbiology, vol. 6: 392, pp. 1-10, May 2015. doi: 10.3389/fmicb.2015.00392.
33. M. Österblad, O. Pensala, M. Peterzéns, H. Heleniusc, and P. Huovinen, “Antimicrobial susceptibility of Enterobactericeae isolated from vegetables,” Journal of Antimicrobial Chemotherapy, vol. 43, pp. 503-509, April, 1999.
34. C. Rodríguez, L. Lang, A. Wang, K. Altendorf, F. García, and A. Lipski, “Lettuce for human consumption collected in Costa Rica contains complex communities of culturable oxytetracycline- and gentamycin-resistant bacteria,” Appl. Environ. Microbiol., vol. 72, pp. 5870-5876, September 2006.
35. R. Marti, A. Scott, Y. Tien, R. Murray, L. Sabourin, et al., “Impact of manure fertilization on the abundance of antibitic-resistant bacteria and frequency of detection of antibiotic resistance genes in soil and on vegetables at harvest,” Appl. Environ. Microbiol., vol. 79 (18), pp. 5701-5709, July 2013. doi: 10.1128/AEM.01682-13.
36. D. A. Abavelim, “Determination of antibiotics residues in beef and mutton from selected markets in Kumasi-Ghana,” MPhil thesis, KNUSTSpace, University of Science and Technology, Kumasi, Ghana, 2014.
37. N. Duran, B. Ozer, G. G. Duran, Y. Onlen, and C. Demir, “Antimicrobial resistance genes and susceptibility patterns in staphylococci,” Indian J. Med. Res., vol. 135, pp. 389-396, March 2012.
38. G. G. Khachatourians, “Agricultural use of antibiotics and evolution and transfer of antibiotic-resistant bacteria,” CMAJ, vol. 159, pp. 1129-36, November 1998.
39. S. Boehme, G. Werner, I. Klare, R. Reissbrodt, and W. Witte, “Occurrence of antibiotic-resistant enterobacteria in agricultural foodstuffs,” Mol. Nutr. Food Res., vol. 48, pp. 522-531, December 2004.
40. Government of Canada, “Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS) 2007”. Public Health Agency of Canada, Guelph, ON, Canada, 2010.
41. L. Łaniewska-Trokenheim, M. Sobota, and I. Warmińska-Radyko, “Antibiotic resistance of bacteria of the family enterobacteriacea isolated from vegetables – short report,” Pol. J. Food Nutr. Sci., vol. 15/56 (4), pp. 427–431, April 2006.
42. A. Mukherjee, D. Speh, and F. Diez-Gonzalez, “Association of farm management practices with risk of Escherichia coli contamination in pre-harvest produce grown in Minnesota and Wisconsin,” Int. J. Food Microbiol., vol. 120, pp. 296-302, December 2007.
43. R. S. Gupta, “Origin of diderm (gram-negative) bacteria: antibiotic selection pressure rather than endosymbiosis likely led to the evolution of bacterial cells with two membranes,” Antonie van Leeuwenhoek, vol. 100, pp. 171–182, August 2011. doi: 10.1007/s10482-011-9616-8. PMC: 3133647.
44. R. Ruimy, A. Brisabois, C. Bernede, D. Skurnik, S. Barnat, et al., “Organic and conventional fruits and vegetables contain equivalent counts of Gram-negative bacteria expressing resistance to antibacterial agents,” Environmental Microbiology, vol. 12 (3), pp. 608–615, March 2010. (doi: 10.1111/j.1462-2920.2009.02100.x).
45. M. Will, and D. Guenther, “Food Quality and Safety Standards as required by EU Law and the Private Industry with special Reference to MEDA Countries’ Exports of Fresh and Processed Fruits and Vegetables, Herbs and Spices. A Practitioners’ Reference Book, 2nd Edition, GTZ – Division 45, January 2007.
46. P. Drechsel, and H. Karg, “Motivating behaviour change for safe waste-water irrigation in urban and peri-urban Ghana,” Sustainable Sanitation Practice, vol. 16, pp. 10-20, March 2013.