Morphometric Characterization of Endomycorrhizal Fungi (Glomeraceae and Acaulosporaceae) from the Bouaflé and Niellé Areas in Côte d'Ivoire

Germain Droh; Kouadio Meliton Djezou; Seydou Tuo; Mamadou Touré; Abou-Bakari Kouassi

doi:doi:10.11648/j.ajbio.20231101.11

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Morphometric Characterization of Endomycorrhizal Fungi (Glomeraceae and Acaulosporaceae) from the Bouaflé and Niellé Areas in Côte d'Ivoire

Germain Droh, Kouadio Meliton Djezou, Seydou Tuo, Mamadou Touré, Abou-Bakari Kouassi

Published in American Journal of BioScience (Volume 11, Issue 1)

Received: 31 October 2022 Accepted: 7 December 2022 Published: 10 January 2023

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Abstract

Mycorrhizal symbioses, which are widespread in various terrestrial ecosystems, constitute a very important research topic for many biologists. Arbuscular mycorrhizal fungi (AMF), belonging to the phylum Glomeromycota, take their name from their characteristic structures: arbuscules. Spores represent the main structures allowing the morphological identification and characterization of AMF. The spores directly extracted from soil samples were identified under an optical microscope. The diversity of AMF in maize rhizospheres in Bouaflé and Niellé areas was highlighted. Thus, 12 genera of AMF divided into 8 families have been identified. Spores of two families of endomycorrhizae (Glomeraceae and Acaulosporaceae) have been described. The spores of the Glomeraceae family represented by 4 genera Glomus, Funneliformis, Septoglomus and Rhizophagus are globose to subglobose, 45 to 200 µm in size. The spores have a single wall composed of 3 to 4 parietal layers. The outer layers stain with Melzer's reagent. The suspensory hypha often characterized by the presence of constriction, light brown in color is the extension of layer 2. Morphologically, the spores of Acaulosporaceae represented by a single genus Acaulospora, are distinguished by the globose to subglobose shape. The spores are whitish, pale yellow, golden yellow to orange-brown in color and 90 to 200µm in diameter. The subcellular structure of spores consists of a spore wall (outer wall) and two inner walls. The outer and inner walls of the spores consist of three parietal layers. The outermost layers are evanescent, hyaline whitish, orange-brown to pale yellow. The layers of the inner wall are adherent to each other appearing as a single layer. The surface of the spores, populated with projections of different shapes resembling often rudimentary hyphae, show scars and ornamentations. This study gives the essential of the status of AMF and revealed the morphometric and structural characteristics of some AMF.

Published in	American Journal of BioScience (Volume 11, Issue 1)
DOI	10.11648/j.ajbio.20231101.11
Page(s)	1-10
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Arbuscular Mycorrhizal Fungi, Glomeraceae, Spore Diversity, Spore Density, Acaulosporaceae

References

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Germain Droh, Kouadio Meliton Djezou, Seydou Tuo, Mamadou Touré, Abou-Bakari Kouassi. (2023). Morphometric Characterization of Endomycorrhizal Fungi (Glomeraceae and Acaulosporaceae) from the Bouaflé and Niellé Areas in Côte d'Ivoire. American Journal of BioScience, 11(1), 1-10. https://doi.org/10.11648/j.ajbio.20231101.11

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Germain Droh; Kouadio Meliton Djezou; Seydou Tuo; Mamadou Touré; Abou-Bakari Kouassi. Morphometric Characterization of Endomycorrhizal Fungi (Glomeraceae and Acaulosporaceae) from the Bouaflé and Niellé Areas in Côte d'Ivoire. Am. J. BioScience 2023, 11(1), 1-10. doi: 10.11648/j.ajbio.20231101.11

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Germain Droh, Kouadio Meliton Djezou, Seydou Tuo, Mamadou Touré, Abou-Bakari Kouassi. Morphometric Characterization of Endomycorrhizal Fungi (Glomeraceae and Acaulosporaceae) from the Bouaflé and Niellé Areas in Côte d'Ivoire. Am J BioScience. 2023;11(1):1-10. doi: 10.11648/j.ajbio.20231101.11

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@article{10.11648/j.ajbio.20231101.11,
  author = {Germain Droh and Kouadio Meliton Djezou and Seydou Tuo and Mamadou Touré and Abou-Bakari Kouassi},
  title = {Morphometric Characterization of Endomycorrhizal Fungi (Glomeraceae and Acaulosporaceae) from the Bouaflé and Niellé Areas in Côte d'Ivoire},
  journal = {American Journal of BioScience},
  volume = {11},
  number = {1},
  pages = {1-10},
  doi = {10.11648/j.ajbio.20231101.11},
  url = {https://doi.org/10.11648/j.ajbio.20231101.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20231101.11},
  abstract = {Mycorrhizal symbioses, which are widespread in various terrestrial ecosystems, constitute a very important research topic for many biologists. Arbuscular mycorrhizal fungi (AMF), belonging to the phylum Glomeromycota, take their name from their characteristic structures: arbuscules. Spores represent the main structures allowing the morphological identification and characterization of AMF. The spores directly extracted from soil samples were identified under an optical microscope. The diversity of AMF in maize rhizospheres in Bouaflé and Niellé areas was highlighted. Thus, 12 genera of AMF divided into 8 families have been identified. Spores of two families of endomycorrhizae (Glomeraceae and Acaulosporaceae) have been described. The spores of the Glomeraceae family represented by 4 genera Glomus, Funneliformis, Septoglomus and Rhizophagus are globose to subglobose, 45 to 200 µm in size. The spores have a single wall composed of 3 to 4 parietal layers. The outer layers stain with Melzer's reagent. The suspensory hypha often characterized by the presence of constriction, light brown in color is the extension of layer 2. Morphologically, the spores of Acaulosporaceae represented by a single genus Acaulospora, are distinguished by the globose to subglobose shape. The spores are whitish, pale yellow, golden yellow to orange-brown in color and 90 to 200µm in diameter. The subcellular structure of spores consists of a spore wall (outer wall) and two inner walls. The outer and inner walls of the spores consist of three parietal layers. The outermost layers are evanescent, hyaline whitish, orange-brown to pale yellow. The layers of the inner wall are adherent to each other appearing as a single layer. The surface of the spores, populated with projections of different shapes resembling often rudimentary hyphae, show scars and ornamentations. This study gives the essential of the status of AMF and revealed the morphometric and structural characteristics of some AMF.},
 year = {2023}
}

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TY - JOUR
T1 - Morphometric Characterization of Endomycorrhizal Fungi (Glomeraceae and Acaulosporaceae) from the Bouaflé and Niellé Areas in Côte d'Ivoire
AU - Germain Droh
AU - Kouadio Meliton Djezou
AU - Seydou Tuo
AU - Mamadou Touré
AU - Abou-Bakari Kouassi
Y1 - 2023/01/10
PY - 2023
N1 - https://doi.org/10.11648/j.ajbio.20231101.11
DO - 10.11648/j.ajbio.20231101.11
T2 - American Journal of BioScience
JF - American Journal of BioScience
JO - American Journal of BioScience
SP - 1
EP - 10
PB - Science Publishing Group
SN - 2330-0167
UR - https://doi.org/10.11648/j.ajbio.20231101.11
AB - Mycorrhizal symbioses, which are widespread in various terrestrial ecosystems, constitute a very important research topic for many biologists. Arbuscular mycorrhizal fungi (AMF), belonging to the phylum Glomeromycota, take their name from their characteristic structures: arbuscules. Spores represent the main structures allowing the morphological identification and characterization of AMF. The spores directly extracted from soil samples were identified under an optical microscope. The diversity of AMF in maize rhizospheres in Bouaflé and Niellé areas was highlighted. Thus, 12 genera of AMF divided into 8 families have been identified. Spores of two families of endomycorrhizae (Glomeraceae and Acaulosporaceae) have been described. The spores of the Glomeraceae family represented by 4 genera Glomus, Funneliformis, Septoglomus and Rhizophagus are globose to subglobose, 45 to 200 µm in size. The spores have a single wall composed of 3 to 4 parietal layers. The outer layers stain with Melzer's reagent. The suspensory hypha often characterized by the presence of constriction, light brown in color is the extension of layer 2. Morphologically, the spores of Acaulosporaceae represented by a single genus Acaulospora, are distinguished by the globose to subglobose shape. The spores are whitish, pale yellow, golden yellow to orange-brown in color and 90 to 200µm in diameter. The subcellular structure of spores consists of a spore wall (outer wall) and two inner walls. The outer and inner walls of the spores consist of three parietal layers. The outermost layers are evanescent, hyaline whitish, orange-brown to pale yellow. The layers of the inner wall are adherent to each other appearing as a single layer. The surface of the spores, populated with projections of different shapes resembling often rudimentary hyphae, show scars and ornamentations. This study gives the essential of the status of AMF and revealed the morphometric and structural characteristics of some AMF.
VL - 11
IS - 1
ER -

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Germain Droh

Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, Faculty of Biosciences, University Félix HOUPHOU?T-BOIGNY, Abidjan, C?te d’Ivoire
Kouadio Meliton Djezou

Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, Faculty of Biosciences, University Félix HOUPHOU?T-BOIGNY, Abidjan, C?te d’Ivoire
Seydou Tuo

Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, Faculty of Biosciences, University Félix HOUPHOU?T-BOIGNY, Abidjan, C?te d’Ivoire
Mamadou Touré

Laboratory of Ecology and Sustainable Development, University of NANGUI ABROGOUA, Abidjan, C?te d’Ivoire
Abou-Bakari Kouassi

Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, Faculty of Biosciences, University Félix HOUPHOU?T-BOIGNY, Abidjan, C?te d’Ivoire

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Germain Droh, Kouadio Meliton Djezou, Seydou Tuo, Mamadou Touré, Abou-Bakari Kouassi. (2023). Morphometric Characterization of Endomycorrhizal Fungi (Glomeraceae and Acaulosporaceae) from the Bouaflé and Niellé Areas in Côte d'Ivoire. American Journal of BioScience, 11(1), 1-10. https://doi.org/10.11648/j.ajbio.20231101.11

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Germain Droh; Kouadio Meliton Djezou; Seydou Tuo; Mamadou Touré; Abou-Bakari Kouassi. Morphometric Characterization of Endomycorrhizal Fungi (Glomeraceae and Acaulosporaceae) from the Bouaflé and Niellé Areas in Côte d'Ivoire. Am. J. BioScience 2023, 11(1), 1-10. doi: 10.11648/j.ajbio.20231101.11

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Germain Droh, Kouadio Meliton Djezou, Seydou Tuo, Mamadou Touré, Abou-Bakari Kouassi. Morphometric Characterization of Endomycorrhizal Fungi (Glomeraceae and Acaulosporaceae) from the Bouaflé and Niellé Areas in Côte d'Ivoire. Am J BioScience. 2023;11(1):1-10. doi: 10.11648/j.ajbio.20231101.11

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@article{10.11648/j.ajbio.20231101.11,
  author = {Germain Droh and Kouadio Meliton Djezou and Seydou Tuo and Mamadou Touré and Abou-Bakari Kouassi},
  title = {Morphometric Characterization of Endomycorrhizal Fungi (Glomeraceae and Acaulosporaceae) from the Bouaflé and Niellé Areas in Côte d'Ivoire},
  journal = {American Journal of BioScience},
  volume = {11},
  number = {1},
  pages = {1-10},
  doi = {10.11648/j.ajbio.20231101.11},
  url = {https://doi.org/10.11648/j.ajbio.20231101.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20231101.11},
  abstract = {Mycorrhizal symbioses, which are widespread in various terrestrial ecosystems, constitute a very important research topic for many biologists. Arbuscular mycorrhizal fungi (AMF), belonging to the phylum Glomeromycota, take their name from their characteristic structures: arbuscules. Spores represent the main structures allowing the morphological identification and characterization of AMF. The spores directly extracted from soil samples were identified under an optical microscope. The diversity of AMF in maize rhizospheres in Bouaflé and Niellé areas was highlighted. Thus, 12 genera of AMF divided into 8 families have been identified. Spores of two families of endomycorrhizae (Glomeraceae and Acaulosporaceae) have been described. The spores of the Glomeraceae family represented by 4 genera Glomus, Funneliformis, Septoglomus and Rhizophagus are globose to subglobose, 45 to 200 µm in size. The spores have a single wall composed of 3 to 4 parietal layers. The outer layers stain with Melzer's reagent. The suspensory hypha often characterized by the presence of constriction, light brown in color is the extension of layer 2. Morphologically, the spores of Acaulosporaceae represented by a single genus Acaulospora, are distinguished by the globose to subglobose shape. The spores are whitish, pale yellow, golden yellow to orange-brown in color and 90 to 200µm in diameter. The subcellular structure of spores consists of a spore wall (outer wall) and two inner walls. The outer and inner walls of the spores consist of three parietal layers. The outermost layers are evanescent, hyaline whitish, orange-brown to pale yellow. The layers of the inner wall are adherent to each other appearing as a single layer. The surface of the spores, populated with projections of different shapes resembling often rudimentary hyphae, show scars and ornamentations. This study gives the essential of the status of AMF and revealed the morphometric and structural characteristics of some AMF.},
 year = {2023}
}

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