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Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract

Received: 20 March 2017     Accepted: 31 March 2017     Published: 8 May 2017
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Abstract

Withania somnifera (ashwagandha) root extract is a popular health supplement, with purported health benefits including prevention and treatment of various diseases, i.e. anxiety, stress, etc. The objective of this experiment was to evaluate the impact of Energy of Consciousness Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The liquid chromatographic data of the control and Biofield Energy Treated samples revealed that the retention time of the 25 chromatographic peaks remained same, whereas the peak area% i.e. the relative amount of the phytoconstituents was altered significantly. The peak area% of the treated ashwagandha root extract representing the phytoconstituents was significantly decreased in the range of 6.02% to 39.74% at Rt of 5.2, 5.3, 5.4, 5.6, 5.7, 6.9, 7.1, 7.3, 7.8, 7.9, 8.0, and 8.5 minutes compared to the control sample. On the contrary, the peak area% of the other phytoconstituents present in the treated sample was significantly increased in the range of 4.12% to 82.32% at Rt of 5.1, 6.4, 6.6, 6.8, 8.1, 8.2, 8.4, 8.6, 8.8, 8.9, 9.0, 9.1, and 9.2 minutes, respectively compared to the control sample. A total of 16 withanolides such as sitoindoside IX, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, ixocarpalactone A, withanolide sulfoxide, etc. were proposed from the molecular mass at m/z 605, 489, 473, 471, 505, and 992 at the retention times of 6.9, 7.1, 7.8, 8.2, 8.5, and 9.2 minutes with the help of LC-MS, GC-MS and NMR data of both the samples. Subsequently, the mass peak intensities of the treated sample were significantly changed in the range of -61.24% to 106.61% compared with the control sample at the same retention time. These findings suggest that Energy of Consciousness Healing Treatment could be advantageous for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, stress, cancer, diabetes, sexual disorders, aging, and other chronic infections.

Published in European Journal of Biophysics (Volume 5, Issue 2)
DOI 10.11648/j.ejb.20170502.12
Page(s) 38-47
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Withania somnifera, The Trivedi Effect®, Biofield Energy Healers, Energy of Consciousness Healing Treatment, Withanolides, LC-MS, GC-MS

References
[1] Kesarwani K, Gupta R (2013) Bioavailability enhancers of herbal origin: An overview. Asian Pac J Trop Biomed 3: 253-266.
[2] Singh N, Bhalla M, Jager P, Gilca M (2011) An overview on ashwagandha: A rasayana (rejuvenator) of Ayurveda. Afr J Tradit Complement Altern Med 8: 208-213.
[3] Kulkarni SK, Dhir A (2008) Withania somnifera: An Indian ginseng. Prog Neuropsychopharmacol Biol Psychiatry 32: 1093-1105.
[4] Dar NJ, Hamid A, Ahmad M (2015) Pharmacologic overview of Withania somnifera, the Indian ginseng. Cell Mol Life Sci 72: 4445-4460.
[5] Mir BA, Khazir J, Mir NA, Hasan T-ul, Koul S (2012) Botanical, chemical and pharmacological review of Withania somnifera (Indian ginseng): An Ayurvedic medicinal plant. Indian J Drugs Dis 1: 147-160.
[6] Mishra LC, Singh BB, Dagenais S (2000) Scientific basis for the therapeutic use of Withania somnifera (Ashwagandha): A review. Altern Med Rev 5: 334-346.
[7] Kumar V, Dey A, Hadimani MB, Marcović T, Emerald M (2015) Chemistry and pharmacology of Withania somnifera: An update. Tang (Humanitas Medicine) 5: e1.
[8] Bolleddula J, Fitch W, Vareed SK, Nair MG (2012) Identification of metabolites in Withania sominfera fruits by liquid chromatography and high-resolution mass spectrometry. Rapid Commun Mass Spectrom 26: 1277-1290.
[9] Mirjalili MH, Moyano E, Bonfill M, Cusido RM, Palazón J (2009) Steroidal lactones from Withania somnifera, an ancient plant for novel medicine. Molecules 14: 2373-2393.
[10] Singh A, Duggal S, Singh H, Singh J, Katekhaye S (2010) Withanolides: Phytoconstituents with significant pharmacological activities. Int J Green Pharm 4: 229-237.
[11] Chen LX, He H, Qiu F (2011) Natural withanolides: An overview. Nat Prod Rep 28: 705-740.
[12] Budhiraja RD, Krishan P, Sudhir S (2000) Biological activity of withanolides. J Sci Ind Res 59: 904-911.
[13] Stenger VJ (1999) Bioenergetic fields. Sci Rev Alternative Med 3.
[14] Rogers, M (1989) "Nursing: A Science of Unitary Human Beings." In J. P. Riehl-Sisca (ed.) Conceptual Models for Nursing Practice. 3rd edition. Norwark: Appleton & Lange.
[15] Rosa L, Rosa E, Sarner L, Barrett S (1998) A close look at therapeutic touch. JAMA 279: 1005-1010.
[16] Warber SL, Cornelio D, Straughn, J, Kile G (2004) Biofield energy healing from the inside. J Altern Complement Med 10: 1107-1113.
[17] Nelson LA, Schwartz GE (2005) Human biofield and intention detection: Individual differences. J Altern Complement Med 11: 93-101.
[18] Nemeth L (2008) Energy and biofield therapies in practice. Beginnings. Summer 28: 4-5.
[19] Koithan M (2009) Introducing complementary and alternative therapies. J Nurse Pract 5: 18-20.
[20] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4: 141.
[21] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In vitro evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther 7: 253-257.
[22] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of antibiogram, genotype and phylogenetic analysis of biofield treated Nocardia otitidis. Biol Syst Open Access 4: 143.
[23] Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Antibiogram, biochemical reactions, and genotypic pattern of biofield treated Pseudomonas aeruginosa. J Trop Dis 4: 181.
[24] Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Bacterial identification using 16S rDNA gene sequencing and antibiogram analysis on biofield treated Pseudomonas fluorescens. Clin Med Biochemistry Open Access 1: 101.
[25] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In vitro evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther 7: 253-257.
[26] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Antimicrobial sensitivity, biochemical characteristics and biotyping of Staphylococcus saprophyticus: An impact of biofield energy treatment. J Women’s Health Care 4: 271.
[27] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Effect of biofield treatment on antimicrobials susceptibility pattern of Acinetobacter baumannii - An experimental study. J Clin Diagn Res 3: 1.
[28] Trivedi MK, Patil S, Tallapragada RM (2013) Effect of biofield treatment on the physical and thermal characteristics of vanadium pentoxide powders. J Material Sci Eng S 11: 001.
[29] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Characterization of physical and structural properties of aluminum carbide powder: Impact of biofield treatment. J Aeronaut Aerospace Eng 4: 142.
[30] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) The potential impact of biofield energy treatment on the atomic and physical properties of antimony tin oxide nanopowder. American Journal of Optics and Photonics 3: 123-128.
[31] Trivedi MK, Branton A, Trivedi D, Nayak G, Panda P, Jana S (2016) Gas chromatography-mass spectrometric analysis of isotopic abundance of 13C, 2H, and 18O in biofield energy treated p-tertiary butylphenol (PTBP). American Journal of Chemical Engineering 4: 78-86.
[32] Trivedi MK, Branton A, Trivedi D, Nayak G, Sethi KK, Jana S (2016) Gas chromatography-mass spectrometry based isotopic abundance ratio analysis of biofield energy treated methyl-2-napthylether (Nerolin). American Journal of Physical Chemistry 5: 80-86.
[33] Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, Jana S (2015) Spectroscopic characterization of disodium hydrogen orthophosphate and sodium nitrate after biofield treatment. J Chromatogr Sep Tech 6: 282.
[34] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Potential impact of biofield treatment on atomic and physical characteristics of magnesium. Vitam Miner 3: 129.
[35] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mishra RK, Jana S (2015) Biofield treatment: A potential strategy for modification of physical and thermal properties of gluten hydrolysate and ipomoea macroelements. J Nutr Food Sci 5: 414.
[36] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Effect of biofield treatment on spectral properties of paracetamol and piroxicam. Chem Sci J 6: 98.
[37] Trivedi MK, Patil S, Shettigar H, Singh R, Jana S (2015) An impact of biofield treatment on spectroscopic characterization of pharmaceutical compounds. Mod Chem Appl 3: 159.
[38] Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Agronomic characteristics, growth analysis, and yield response of biofield treated mustard, cowpea, horse gram, and groundnuts. International Journal of Genetics and Genomics. 3: 74-80.
[39] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of plant growth, yield and yield attributes of biofield energy treated mustard (brassica juncea) and chick pea (Cicer arietinum) seeds. Agriculture, Forestry and Fisheries. 4: 291-295.
[40] Chatterjee S, Srivastava S, Khalid A, Singh N, Sangwan RS, Sidhu OP, Roy R, Khetrapal CL, Tuli R (2010) Comprehensive metabolic fingerprinting of Withania somnifera leaf and root extracts. Phytochemistry 71: 1085-1094.
[41] Chaurasiya ND, Uniyal GC, Lal P, Misra L, Sangwan NS, Tuli R, Sangwan RS (2008) Analysis of withanolides in root and leaf of Withania somnifera by HPLC with photodiode array and evaporative light scattering detection. Phytochem Anal 19: 148-154.
[42] Musharraf SG, Ali A, Ali RA, Yousuf S, Rahman AU, Choudhary MI (2011) Analysis and development of structure-fragmentation relationships in withanolides using an electrospray ionization quadropole time-of-flight tandem mass spectrometry hybrid instrument. Rapid Commun Mass Spectrom 25: 104-114.
[43] Trivedi MK, Panda P, Sethi KK, Jana S (2017) Metabolite profiling of Withania somnifera roots hydroalcoholic extract using LC-MS, GC-MS and NMR spectroscopy. Chem Biodivers 14: e1600280.
[44] https://www.scribd.com/document/220440935/Theory-of-HPLC-Quantitative-and-Qualitative-HPLC.
[45] Mulabagal V, Subbaraju GV, Rao CV, Sivaramakrishna C, Dewitt DL, Holmes D, Sung B, Aggarwal BB, Tsay HS, Nair MG (2009) Withanolide sulfoxide from Aswagandha roots inhibits nuclear transcription factor-B, cyclooxygenase and tumor cell proliferation. Phytother Res 23: 987-992.
Cite This Article
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    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, et al. (2017). Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract. European Journal of Biophysics, 5(2), 38-47. https://doi.org/10.11648/j.ejb.20170502.12

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    ACS Style

    Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; William Dean Plikerd, et al. Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract. Eur. J. Biophys. 2017, 5(2), 38-47. doi: 10.11648/j.ejb.20170502.12

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    AMA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, et al. Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract. Eur J Biophys. 2017;5(2):38-47. doi: 10.11648/j.ejb.20170502.12

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  • @article{10.11648/j.ejb.20170502.12,
      author = {Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and William Dean Plikerd and Peter L. Surguy and Robert John Kock and Rolando Baptista Piedad and Russell Phillip Callas and Sakina A. Ansari and Sandra Lee Barrett and Sara Friedman and Steven Lee Christie and Su-Mei Chen Liu and Susan Elizabeth Starling and Susan Jones and Susan Mardis Allen and Susanne Kathrin Wasmus and Terry Ann Benczik and Thomas Charles Slade and Thomas Orban and Victoria L. Vannes and Victoria Margot Schlosser and Yusif Sarkis Yamin Albino and Kalyan Kumar Sethi and Parthasarathi Panda and Snehasis Jana},
      title = {Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract},
      journal = {European Journal of Biophysics},
      volume = {5},
      number = {2},
      pages = {38-47},
      doi = {10.11648/j.ejb.20170502.12},
      url = {https://doi.org/10.11648/j.ejb.20170502.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejb.20170502.12},
      abstract = {Withania somnifera (ashwagandha) root extract is a popular health supplement, with purported health benefits including prevention and treatment of various diseases, i.e. anxiety, stress, etc. The objective of this experiment was to evaluate the impact of Energy of Consciousness Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The liquid chromatographic data of the control and Biofield Energy Treated samples revealed that the retention time of the 25 chromatographic peaks remained same, whereas the peak area% i.e. the relative amount of the phytoconstituents was altered significantly. The peak area% of the treated ashwagandha root extract representing the phytoconstituents was significantly decreased in the range of 6.02% to 39.74% at Rt of 5.2, 5.3, 5.4, 5.6, 5.7, 6.9, 7.1, 7.3, 7.8, 7.9, 8.0, and 8.5 minutes compared to the control sample. On the contrary, the peak area% of the other phytoconstituents present in the treated sample was significantly increased in the range of 4.12% to 82.32% at Rt of 5.1, 6.4, 6.6, 6.8, 8.1, 8.2, 8.4, 8.6, 8.8, 8.9, 9.0, 9.1, and 9.2 minutes, respectively compared to the control sample. A total of 16 withanolides such as sitoindoside IX, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, ixocarpalactone A, withanolide sulfoxide, etc. were proposed from the molecular mass at m/z 605, 489, 473, 471, 505, and 992 at the retention times of 6.9, 7.1, 7.8, 8.2, 8.5, and 9.2 minutes with the help of LC-MS, GC-MS and NMR data of both the samples. Subsequently, the mass peak intensities of the treated sample were significantly changed in the range of -61.24% to 106.61% compared with the control sample at the same retention time. These findings suggest that Energy of Consciousness Healing Treatment could be advantageous for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, stress, cancer, diabetes, sexual disorders, aging, and other chronic infections.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract
    AU  - Mahendra Kumar Trivedi
    AU  - Alice Branton
    AU  - Dahryn Trivedi
    AU  - Gopal Nayak
    AU  - William Dean Plikerd
    AU  - Peter L. Surguy
    AU  - Robert John Kock
    AU  - Rolando Baptista Piedad
    AU  - Russell Phillip Callas
    AU  - Sakina A. Ansari
    AU  - Sandra Lee Barrett
    AU  - Sara Friedman
    AU  - Steven Lee Christie
    AU  - Su-Mei Chen Liu
    AU  - Susan Elizabeth Starling
    AU  - Susan Jones
    AU  - Susan Mardis Allen
    AU  - Susanne Kathrin Wasmus
    AU  - Terry Ann Benczik
    AU  - Thomas Charles Slade
    AU  - Thomas Orban
    AU  - Victoria L. Vannes
    AU  - Victoria Margot Schlosser
    AU  - Yusif Sarkis Yamin Albino
    AU  - Kalyan Kumar Sethi
    AU  - Parthasarathi Panda
    AU  - Snehasis Jana
    Y1  - 2017/05/08
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ejb.20170502.12
    DO  - 10.11648/j.ejb.20170502.12
    T2  - European Journal of Biophysics
    JF  - European Journal of Biophysics
    JO  - European Journal of Biophysics
    SP  - 38
    EP  - 47
    PB  - Science Publishing Group
    SN  - 2329-1737
    UR  - https://doi.org/10.11648/j.ejb.20170502.12
    AB  - Withania somnifera (ashwagandha) root extract is a popular health supplement, with purported health benefits including prevention and treatment of various diseases, i.e. anxiety, stress, etc. The objective of this experiment was to evaluate the impact of Energy of Consciousness Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The liquid chromatographic data of the control and Biofield Energy Treated samples revealed that the retention time of the 25 chromatographic peaks remained same, whereas the peak area% i.e. the relative amount of the phytoconstituents was altered significantly. The peak area% of the treated ashwagandha root extract representing the phytoconstituents was significantly decreased in the range of 6.02% to 39.74% at Rt of 5.2, 5.3, 5.4, 5.6, 5.7, 6.9, 7.1, 7.3, 7.8, 7.9, 8.0, and 8.5 minutes compared to the control sample. On the contrary, the peak area% of the other phytoconstituents present in the treated sample was significantly increased in the range of 4.12% to 82.32% at Rt of 5.1, 6.4, 6.6, 6.8, 8.1, 8.2, 8.4, 8.6, 8.8, 8.9, 9.0, 9.1, and 9.2 minutes, respectively compared to the control sample. A total of 16 withanolides such as sitoindoside IX, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, ixocarpalactone A, withanolide sulfoxide, etc. were proposed from the molecular mass at m/z 605, 489, 473, 471, 505, and 992 at the retention times of 6.9, 7.1, 7.8, 8.2, 8.5, and 9.2 minutes with the help of LC-MS, GC-MS and NMR data of both the samples. Subsequently, the mass peak intensities of the treated sample were significantly changed in the range of -61.24% to 106.61% compared with the control sample at the same retention time. These findings suggest that Energy of Consciousness Healing Treatment could be advantageous for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, stress, cancer, diabetes, sexual disorders, aging, and other chronic infections.
    VL  - 5
    IS  - 2
    ER  - 

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Author Information
  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India

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