Amaranthus hybridus L. is an important vegetable in Nigeria, grown mainly for its highly nutritious leaves. In a bid to explore the possibility of increasing the infra-specific diversity of the crop, we induced three accessions of A. hybridus from Anambra State, Nigeria with x-ray particles using various concentration levels. The experimental layout composed a 5 x 3 x 3 Randomized Complete Block Design arrangement, with treatment levels: 1 MGY, 2 MGY, 4 MGY, and 6 MGY, selectively allotted to the different accession based on a preliminary dosimetry test. Morphological data were recorded and subsequently leaves samples subjected to Deoxyribonucleic Acid (DNA) extraction and Random Amplified Polymorphic DNA (RAPD) analysis. The results show that except for inflorescence weight, other morphological data were not statistically significant. However, RAPD techniques identify some variations induced by x-ray particles on the different accessions. Ogbunike accession irradiated with 4 MGY appears to show the optimum concentration of x-ray particles required to induce genetic variability in A. hybridus. These findings are of paramount importance to plant breeders, whose primary tool is to identify variations to produce better yielding varieties.
| Published in | International Journal of Genetics and Genomics (Volume 7, Issue 2) |
| DOI | 10.11648/j.ijgg.20190702.11 |
| Page(s) | 18-26 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
X-Ray, Amaranthus hybridus L., Genetic Variability, Mutation, Diversity, RAPD
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APA Style
Beluchukwu Joseph Nwankwo, Garuba Omosun, Hilary Odo Edeoga, Odoligie Imarhiagbe, Jeffrey Iyare Omoruyi, et al. (2019). X-ray Induced Genetic Variability in Amaranthus hybridus L. and Analysis of Variants Using Morphological and Random Amplified Polymorphic DNA Data. International Journal of Genetics and Genomics, 7(2), 18-26. https://doi.org/10.11648/j.ijgg.20190702.11
ACS Style
Beluchukwu Joseph Nwankwo; Garuba Omosun; Hilary Odo Edeoga; Odoligie Imarhiagbe; Jeffrey Iyare Omoruyi, et al. X-ray Induced Genetic Variability in Amaranthus hybridus L. and Analysis of Variants Using Morphological and Random Amplified Polymorphic DNA Data. Int. J. Genet. Genomics 2019, 7(2), 18-26. doi: 10.11648/j.ijgg.20190702.11
AMA Style
Beluchukwu Joseph Nwankwo, Garuba Omosun, Hilary Odo Edeoga, Odoligie Imarhiagbe, Jeffrey Iyare Omoruyi, et al. X-ray Induced Genetic Variability in Amaranthus hybridus L. and Analysis of Variants Using Morphological and Random Amplified Polymorphic DNA Data. Int J Genet Genomics. 2019;7(2):18-26. doi: 10.11648/j.ijgg.20190702.11
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Download@article{10.11648/j.ijgg.20190702.11,
author = {Beluchukwu Joseph Nwankwo and Garuba Omosun and Hilary Odo Edeoga and Odoligie Imarhiagbe and Jeffrey Iyare Omoruyi and Edeji Franklin Uzodinma},
title = {X-ray Induced Genetic Variability in Amaranthus hybridus L. and Analysis of Variants Using Morphological and Random Amplified Polymorphic DNA Data},
journal = {International Journal of Genetics and Genomics},
volume = {7},
number = {2},
pages = {18-26},
doi = {10.11648/j.ijgg.20190702.11},
url = {https://doi.org/10.11648/j.ijgg.20190702.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20190702.11},
abstract = {Amaranthus hybridus L. is an important vegetable in Nigeria, grown mainly for its highly nutritious leaves. In a bid to explore the possibility of increasing the infra-specific diversity of the crop, we induced three accessions of A. hybridus from Anambra State, Nigeria with x-ray particles using various concentration levels. The experimental layout composed a 5 x 3 x 3 Randomized Complete Block Design arrangement, with treatment levels: 1 MGY, 2 MGY, 4 MGY, and 6 MGY, selectively allotted to the different accession based on a preliminary dosimetry test. Morphological data were recorded and subsequently leaves samples subjected to Deoxyribonucleic Acid (DNA) extraction and Random Amplified Polymorphic DNA (RAPD) analysis. The results show that except for inflorescence weight, other morphological data were not statistically significant. However, RAPD techniques identify some variations induced by x-ray particles on the different accessions. Ogbunike accession irradiated with 4 MGY appears to show the optimum concentration of x-ray particles required to induce genetic variability in A. hybridus. These findings are of paramount importance to plant breeders, whose primary tool is to identify variations to produce better yielding varieties.},
year = {2019}
}
TY - JOUR T1 - X-ray Induced Genetic Variability in Amaranthus hybridus L. and Analysis of Variants Using Morphological and Random Amplified Polymorphic DNA Data AU - Beluchukwu Joseph Nwankwo AU - Garuba Omosun AU - Hilary Odo Edeoga AU - Odoligie Imarhiagbe AU - Jeffrey Iyare Omoruyi AU - Edeji Franklin Uzodinma Y1 - 2019/06/26 PY - 2019 N1 - https://doi.org/10.11648/j.ijgg.20190702.11 DO - 10.11648/j.ijgg.20190702.11 T2 - International Journal of Genetics and Genomics JF - International Journal of Genetics and Genomics JO - International Journal of Genetics and Genomics SP - 18 EP - 26 PB - Science Publishing Group SN - 2376-7359 UR - https://doi.org/10.11648/j.ijgg.20190702.11 AB - Amaranthus hybridus L. is an important vegetable in Nigeria, grown mainly for its highly nutritious leaves. In a bid to explore the possibility of increasing the infra-specific diversity of the crop, we induced three accessions of A. hybridus from Anambra State, Nigeria with x-ray particles using various concentration levels. The experimental layout composed a 5 x 3 x 3 Randomized Complete Block Design arrangement, with treatment levels: 1 MGY, 2 MGY, 4 MGY, and 6 MGY, selectively allotted to the different accession based on a preliminary dosimetry test. Morphological data were recorded and subsequently leaves samples subjected to Deoxyribonucleic Acid (DNA) extraction and Random Amplified Polymorphic DNA (RAPD) analysis. The results show that except for inflorescence weight, other morphological data were not statistically significant. However, RAPD techniques identify some variations induced by x-ray particles on the different accessions. Ogbunike accession irradiated with 4 MGY appears to show the optimum concentration of x-ray particles required to induce genetic variability in A. hybridus. These findings are of paramount importance to plant breeders, whose primary tool is to identify variations to produce better yielding varieties. VL - 7 IS - 2 ER -
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