A liquid crystal / fluorescence dual-signal immunosensor is developed for the sensitive detection of aflatoxin B1 (AFB1). Through click chemistry reaction, DNA probe is covalently conjugated to the antibody, constructing a detection system with liquid crystal (LC)/fluorescence dual-signal output capabilities. After co-incubation of DNA-antibody (Ab) conjugate, active DNA, and AFB1, the mixture is transferred to 96-well plates that have been pre-functionalized with AFB1 antigen for competitive binding. In the presence of AFB1, the separated supernatant can induce alignment changes in LC molecules, while the DNA-Ab complexes captured on the surface of 96-well plates can activate CRISPR/Cas12a to cleavage the ssDNA reporter, generating fluorescent signals. Notably, the active DNA demonstrated dual functionality. It can not only hybridize with DNA-antibody conjugate but also activate the CRISPR/Cas12a to cleavage the ssDNA reporter, generating fluorescent signals. As AFB1 concentrations increase, the reduced capture of DNA-Ab complexes on the 96-well plates leads to an increase in DNA and DNA-antibody complexes in the supernatant. It causes the LC image change from dark to bright, while the decreased capture of activator DNA results in the decrease of fluorescence signals. Overall, this dual-signal sensor exhibits high specificity, stability, and reproducibility, with simple operation enabling rapid AFB1 detection, it has the potential of application in real samples.
| Published in | Modern Chemistry (Volume 13, Issue 1) |
| DOI | 10.11648/j.mc.20251301.12 |
| Page(s) | 9-13 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2025. Published by Science Publishing Group |
DNA-Antibody Conjugate, Aflatoxin B1, CRISPR/Cas12a, Liquid Crystal, Fluorescence
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APA Style
Zhu, L., Hu, Q., Zhao, R. (2025). The Construction of Liquid Crystal / Fluorescence Dual-Signal Immunosensor Based on DNA-Antibody Conjugate. Modern Chemistry, 13(1), 9-13. https://doi.org/10.11648/j.mc.20251301.12
ACS Style
Zhu, L.; Hu, Q.; Zhao, R. The Construction of Liquid Crystal / Fluorescence Dual-Signal Immunosensor Based on DNA-Antibody Conjugate. Mod. Chem. 2025, 13(1), 9-13. doi: 10.11648/j.mc.20251301.12
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Download@article{10.11648/j.mc.20251301.12,
author = {Lijuan Zhu and Qiongzheng Hu and Ru-Song Zhao},
title = {The Construction of Liquid Crystal / Fluorescence Dual-Signal Immunosensor Based on DNA-Antibody Conjugate
},
journal = {Modern Chemistry},
volume = {13},
number = {1},
pages = {9-13},
doi = {10.11648/j.mc.20251301.12},
url = {https://doi.org/10.11648/j.mc.20251301.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.mc.20251301.12},
abstract = {A liquid crystal / fluorescence dual-signal immunosensor is developed for the sensitive detection of aflatoxin B1 (AFB1). Through click chemistry reaction, DNA probe is covalently conjugated to the antibody, constructing a detection system with liquid crystal (LC)/fluorescence dual-signal output capabilities. After co-incubation of DNA-antibody (Ab) conjugate, active DNA, and AFB1, the mixture is transferred to 96-well plates that have been pre-functionalized with AFB1 antigen for competitive binding. In the presence of AFB1, the separated supernatant can induce alignment changes in LC molecules, while the DNA-Ab complexes captured on the surface of 96-well plates can activate CRISPR/Cas12a to cleavage the ssDNA reporter, generating fluorescent signals. Notably, the active DNA demonstrated dual functionality. It can not only hybridize with DNA-antibody conjugate but also activate the CRISPR/Cas12a to cleavage the ssDNA reporter, generating fluorescent signals. As AFB1 concentrations increase, the reduced capture of DNA-Ab complexes on the 96-well plates leads to an increase in DNA and DNA-antibody complexes in the supernatant. It causes the LC image change from dark to bright, while the decreased capture of activator DNA results in the decrease of fluorescence signals. Overall, this dual-signal sensor exhibits high specificity, stability, and reproducibility, with simple operation enabling rapid AFB1 detection, it has the potential of application in real samples.
},
year = {2025}
}
TY - JOUR T1 - The Construction of Liquid Crystal / Fluorescence Dual-Signal Immunosensor Based on DNA-Antibody Conjugate AU - Lijuan Zhu AU - Qiongzheng Hu AU - Ru-Song Zhao Y1 - 2025/05/22 PY - 2025 N1 - https://doi.org/10.11648/j.mc.20251301.12 DO - 10.11648/j.mc.20251301.12 T2 - Modern Chemistry JF - Modern Chemistry JO - Modern Chemistry SP - 9 EP - 13 PB - Science Publishing Group SN - 2329-180X UR - https://doi.org/10.11648/j.mc.20251301.12 AB - A liquid crystal / fluorescence dual-signal immunosensor is developed for the sensitive detection of aflatoxin B1 (AFB1). Through click chemistry reaction, DNA probe is covalently conjugated to the antibody, constructing a detection system with liquid crystal (LC)/fluorescence dual-signal output capabilities. After co-incubation of DNA-antibody (Ab) conjugate, active DNA, and AFB1, the mixture is transferred to 96-well plates that have been pre-functionalized with AFB1 antigen for competitive binding. In the presence of AFB1, the separated supernatant can induce alignment changes in LC molecules, while the DNA-Ab complexes captured on the surface of 96-well plates can activate CRISPR/Cas12a to cleavage the ssDNA reporter, generating fluorescent signals. Notably, the active DNA demonstrated dual functionality. It can not only hybridize with DNA-antibody conjugate but also activate the CRISPR/Cas12a to cleavage the ssDNA reporter, generating fluorescent signals. As AFB1 concentrations increase, the reduced capture of DNA-Ab complexes on the 96-well plates leads to an increase in DNA and DNA-antibody complexes in the supernatant. It causes the LC image change from dark to bright, while the decreased capture of activator DNA results in the decrease of fluorescence signals. Overall, this dual-signal sensor exhibits high specificity, stability, and reproducibility, with simple operation enabling rapid AFB1 detection, it has the potential of application in real samples. VL - 13 IS - 1 ER -
Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Jinan, China
Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Jinan, China
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