Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early <i>Dictyostelium</i> Development Upon Starvation

Hideo Taniura; Shuhei Soeda; Ai Fujii; Mio Morioka; Minori Nakamura; Yui Sano

doi:doi:10.11648/j.cb.20210901.11

| Peer-Reviewed

Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation

Hideo Taniura, Shuhei Soeda, Ai Fujii, Mio Morioka, Minori Nakamura, Yui Sano

Published in Cell Biology (Volume 9, Issue 1)

Received: 4 January 2021 Accepted: 18 January 2021 Published: 25 January 2021

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Sirtuin involves in cellular processes to adapt to starvation in the endocrine and metabolic systems. We examined Sir2B function during early Dictyostelium development upon starvation. GFP-tagged Sir2B was expressed and the immunoreactivity was detected a punctate pattern near cell surface. Sir2B mRNA was expressed in vegetative cells and peaked at 2 h after starvation. Ectopic expression of Sir2B enhanced aggregate formation with increased expressions of cell adhesion molecules such as cadA, csaA, TgrB1, and TgrC1 at 8h compared to those in control cells. Sir2B null-mutant cells (Sir2B KO cells) were generated and they formed aggregates smaller than those of wild-type cells after 24 h. Because cell-cell adhesion affects aggregation size, cell cohesion assay of Sir2B KO cells showed cell-cell adhesion impairment at 8 and 10 h during development, and the expressions of four cell adhesion molecules were reduced in Sir2B KO cells compared to wild-type cells until 8 h after starvation. When RNAi-mediated cadA or csaA knockdown cells (cadA KD or csaA KD cells) were generated, csaA KD cells formed smaller aggregates and affected the expressions of other cell adhesion molecules to reduce, but cadA KD cells did not show to affect aggregation size and cell adhesion molecules expressions apparently. These results suggest that Sir2B is involved in csaA expression and affects other cell adhesion molecules during early development upon starvation.

Published in	Cell Biology (Volume 9, Issue 1)
DOI	10.11648/j.cb.20210901.11
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Sirtuin, Sir2B, Cell Adhesion, Development

References

[1]	Picard, F., Kurtev, M., Chung, N., Topark-Ngarm, A., Senawong, T., de Oliveira, R. M., Leid, M., McBurney, M. W., Guarente, L. (2004) Sirt1 promotes fat mobilization in white adipocytes by repressing PPAR-γ. Nature 429: 771-776.
[2]	Michan, S., Sinclair, D. (2007) Sirtuins in mammals: insights into their biological function. Biochem. J. 404: 1-13.
[3]	Vassilopoulos, A., Fritz, K. S., Petersen, D. R., Gius, D. (2011) The human sirtuin family: evolutionary divergences and functions. Hum. Genom. 5: 485-496.
[4]	Yamamoto, M., Takahashi, Y. (2018) The essential role of SIRT1 in hypothalamic-pituitary axis. Front. Endocrinol. 9: 605.
[5]	Soeda, S., Taniura, H. (2018) Sirtuin activator and inhibitor affect early Dictyostelium development upon starvation. Cell Biol. 6: 13-19.
[6]	Taniura, H., Soeda, S., Ohta, T., Oki, M., Tsuboi, R. (2019) Sir2D, a Sirtuin family protein, regulates adenylate cyclase A expression through interaction with the MybB transcription factor early in Dictyostelium development upon starvation. Heliyon 3: e01301.
[7]	Katayama, T., Okuwa, T., Yasukawa, H. (2008) Difference in sensitivity to amino acid starvation between the wild type and Sir2A-null mutant of Dictyostelium. Jpn. J. Protozool. 41: 153-158.
[8]	Saxton, R. A., Sabatini, D. M. (2017) mTOR signaling in growth, metabolism, and disease. Cell 168: 960-976.
[9]	Kapahi, P., Kaeberlein, M., Hansen, K. (2017) Dietary restriction and lifespan: Lessons from invertebrate models. Ageing Res. Reviews 39: 3-14.
[10]	Lee, S., Comer, F. I., Sasaki, A., McLeod, I. X., Duong, Y., Okumura, K., Yates III, J. R., Parent, C. A., Firtel, R. A. (2005) TOR complex 2 integrates cell movement during chemotaxis and signal relay in Dictyostelium. Mol. Biol. Cell 16: 4572-4583.
[11]	Jaiswal, P., Kimmel, A. (2019) mTORC1/AMPK responses define a core gene set for developmental cell fate switching. BMC Biol. 17: 58.
[12]	Raisin-Bouffay, C., Jang, W., Caprette, D. R., Gomer, R. H. (2000) A precise group size in Dictyostelium is generated by a cell-counting factor modulating cell-cell adhesion. Mol. Cell 6: 953-959.
[13]	Siu, C. H., Srikanthadevan, S., Wang, J., Hou, L., Chen, G., Xu, X., Thomson, A., Yang, C. (2011) Regulation of spatiotemporal expression of cell-cell adhesion molecules during development of Dictyostelium discoideum. Dev. Growth Differ. 53: 518-527.
[14]	Taniura, H., Tanabe, N., Bando, Y., Arai, N. (2015) Nse1 and Nse4, subunits of the Smc5-Smc6 complex, are involved in Dictyostelium development upon starvation. Dev. Growth Differ. 57: 430-443.
[15]	Levi, S., Polyakov, M., Egelhoff, T. T. (2000) Green fluorescent protein and epitope tag fusion vectors for Dictyostelium discoideum. Plasmid 44: 231-238.
[16]	Martens, H., Novotny, J., Oberstrass, L., Steck, T. L., Postlethwait, P., Nellen, W. (2002) RNAi in Dictostelium: the role of RNA-directed RNA polymerase and double-stranded RNase. Mol. Biol. Cell 13: 445-453.
[17]	Chen, G., Xu, X., Wu, X., Thomson, A., Siu, C-H. (2014) Assembly of the TgrB1-TgrC1 cell adhesion complex during Dictyostelium discoideum development. Biochem. J. 459: 241-249.
[18]	Coates, J. C., Harwood, A. J. (2001) Cell-cell adhesion transduction during Dictyostelium development. J. Cell Sci. 114: 4349-4358.
[19]	Otsuka, H., Van Haastert, P. J. M. (1998) A novel Myb homolog initiates Dictyostelium development by induction of adenylyl cyclase expression. Genes Dev. 12: 1738-1748.
[20]	Schaap, P. (2016) Evolution of developmental signaling in Dictyostelid social amoebas. Curr. Opin. Genet. Dev. 39: 29-34.

Cite This Article

Plain Text BibTeX RIS

APA Style

Hideo Taniura, Shuhei Soeda, Ai Fujii, Mio Morioka, Minori Nakamura, et al. (2021). Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation. Cell Biology, 9(1), 1-6. https://doi.org/10.11648/j.cb.20210901.11

Copy | Download

ACS Style

Hideo Taniura; Shuhei Soeda; Ai Fujii; Mio Morioka; Minori Nakamura, et al. Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation. Cell Biol. 2021, 9(1), 1-6. doi: 10.11648/j.cb.20210901.11

Copy | Download

AMA Style

Hideo Taniura, Shuhei Soeda, Ai Fujii, Mio Morioka, Minori Nakamura, et al. Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation. Cell Biol. 2021;9(1):1-6. doi: 10.11648/j.cb.20210901.11

Copy | Download

@article{10.11648/j.cb.20210901.11,
  author = {Hideo Taniura and Shuhei Soeda and Ai Fujii and Mio Morioka and Minori Nakamura and Yui Sano},
  title = {Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation},
  journal = {Cell Biology},
  volume = {9},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.cb.20210901.11},
  url = {https://doi.org/10.11648/j.cb.20210901.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20210901.11},
  abstract = {Sirtuin involves in cellular processes to adapt to starvation in the endocrine and metabolic systems. We examined Sir2B function during early Dictyostelium development upon starvation. GFP-tagged Sir2B was expressed and the immunoreactivity was detected a punctate pattern near cell surface. Sir2B mRNA was expressed in vegetative cells and peaked at 2 h after starvation. Ectopic expression of Sir2B enhanced aggregate formation with increased expressions of cell adhesion molecules such as cadA, csaA, TgrB1, and TgrC1 at 8h compared to those in control cells. Sir2B null-mutant cells (Sir2B KO cells) were generated and they formed aggregates smaller than those of wild-type cells after 24 h. Because cell-cell adhesion affects aggregation size, cell cohesion assay of Sir2B KO cells showed cell-cell adhesion impairment at 8 and 10 h during development, and the expressions of four cell adhesion molecules were reduced in Sir2B KO cells compared to wild-type cells until 8 h after starvation. When RNAi-mediated cadA or csaA knockdown cells (cadA KD or csaA KD cells) were generated, csaA KD cells formed smaller aggregates and affected the expressions of other cell adhesion molecules to reduce, but cadA KD cells did not show to affect aggregation size and cell adhesion molecules expressions apparently. These results suggest that Sir2B is involved in csaA expression and affects other cell adhesion molecules during early development upon starvation.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation
AU  - Hideo Taniura
AU  - Shuhei Soeda
AU  - Ai Fujii
AU  - Mio Morioka
AU  - Minori Nakamura
AU  - Yui Sano
Y1  - 2021/01/25
PY  - 2021
N1  - https://doi.org/10.11648/j.cb.20210901.11
DO  - 10.11648/j.cb.20210901.11
T2  - Cell Biology
JF  - Cell Biology
JO  - Cell Biology
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2330-0183
UR  - https://doi.org/10.11648/j.cb.20210901.11
AB  - Sirtuin involves in cellular processes to adapt to starvation in the endocrine and metabolic systems. We examined Sir2B function during early Dictyostelium development upon starvation. GFP-tagged Sir2B was expressed and the immunoreactivity was detected a punctate pattern near cell surface. Sir2B mRNA was expressed in vegetative cells and peaked at 2 h after starvation. Ectopic expression of Sir2B enhanced aggregate formation with increased expressions of cell adhesion molecules such as cadA, csaA, TgrB1, and TgrC1 at 8h compared to those in control cells. Sir2B null-mutant cells (Sir2B KO cells) were generated and they formed aggregates smaller than those of wild-type cells after 24 h. Because cell-cell adhesion affects aggregation size, cell cohesion assay of Sir2B KO cells showed cell-cell adhesion impairment at 8 and 10 h during development, and the expressions of four cell adhesion molecules were reduced in Sir2B KO cells compared to wild-type cells until 8 h after starvation. When RNAi-mediated cadA or csaA knockdown cells (cadA KD or csaA KD cells) were generated, csaA KD cells formed smaller aggregates and affected the expressions of other cell adhesion molecules to reduce, but cadA KD cells did not show to affect aggregation size and cell adhesion molecules expressions apparently. These results suggest that Sir2B is involved in csaA expression and affects other cell adhesion molecules during early development upon starvation.
VL  - 9
IS  - 1
ER  -

Copy | Download

Author Information

Hideo Taniura

Laboratory of Neurochemistry, College of Pharmacy, Ritsumeikan University, Kusatsu, Japan
Shuhei Soeda

Laboratory of Neurochemistry, College of Pharmacy, Ritsumeikan University, Kusatsu, Japan
Ai Fujii

Laboratory of Neurochemistry, College of Pharmacy, Ritsumeikan University, Kusatsu, Japan
Mio Morioka

Laboratory of Neurochemistry, College of Pharmacy, Ritsumeikan University, Kusatsu, Japan
Minori Nakamura

Laboratory of Neurochemistry, College of Pharmacy, Ritsumeikan University, Kusatsu, Japan
Yui Sano

Laboratory of Neurochemistry, College of Pharmacy, Ritsumeikan University, Kusatsu, Japan

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Hideo Taniura, Shuhei Soeda, Ai Fujii, Mio Morioka, Minori Nakamura, et al. (2021). Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation. Cell Biology, 9(1), 1-6. https://doi.org/10.11648/j.cb.20210901.11

Copy | Download

ACS Style

Hideo Taniura; Shuhei Soeda; Ai Fujii; Mio Morioka; Minori Nakamura, et al. Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation. Cell Biol. 2021, 9(1), 1-6. doi: 10.11648/j.cb.20210901.11

Copy | Download

AMA Style

Hideo Taniura, Shuhei Soeda, Ai Fujii, Mio Morioka, Minori Nakamura, et al. Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation. Cell Biol. 2021;9(1):1-6. doi: 10.11648/j.cb.20210901.11

Copy | Download

@article{10.11648/j.cb.20210901.11,
  author = {Hideo Taniura and Shuhei Soeda and Ai Fujii and Mio Morioka and Minori Nakamura and Yui Sano},
  title = {Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation},
  journal = {Cell Biology},
  volume = {9},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.cb.20210901.11},
  url = {https://doi.org/10.11648/j.cb.20210901.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20210901.11},
  abstract = {Sirtuin involves in cellular processes to adapt to starvation in the endocrine and metabolic systems. We examined Sir2B function during early Dictyostelium development upon starvation. GFP-tagged Sir2B was expressed and the immunoreactivity was detected a punctate pattern near cell surface. Sir2B mRNA was expressed in vegetative cells and peaked at 2 h after starvation. Ectopic expression of Sir2B enhanced aggregate formation with increased expressions of cell adhesion molecules such as cadA, csaA, TgrB1, and TgrC1 at 8h compared to those in control cells. Sir2B null-mutant cells (Sir2B KO cells) were generated and they formed aggregates smaller than those of wild-type cells after 24 h. Because cell-cell adhesion affects aggregation size, cell cohesion assay of Sir2B KO cells showed cell-cell adhesion impairment at 8 and 10 h during development, and the expressions of four cell adhesion molecules were reduced in Sir2B KO cells compared to wild-type cells until 8 h after starvation. When RNAi-mediated cadA or csaA knockdown cells (cadA KD or csaA KD cells) were generated, csaA KD cells formed smaller aggregates and affected the expressions of other cell adhesion molecules to reduce, but cadA KD cells did not show to affect aggregation size and cell adhesion molecules expressions apparently. These results suggest that Sir2B is involved in csaA expression and affects other cell adhesion molecules during early development upon starvation.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Sir2B, A Sirtuin Family Protein, Involves in Cell Adhesion Molecule Expression During Early Dictyostelium Development Upon Starvation
AU  - Hideo Taniura
AU  - Shuhei Soeda
AU  - Ai Fujii
AU  - Mio Morioka
AU  - Minori Nakamura
AU  - Yui Sano
Y1  - 2021/01/25
PY  - 2021
N1  - https://doi.org/10.11648/j.cb.20210901.11
DO  - 10.11648/j.cb.20210901.11
T2  - Cell Biology
JF  - Cell Biology
JO  - Cell Biology
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2330-0183
UR  - https://doi.org/10.11648/j.cb.20210901.11
AB  - Sirtuin involves in cellular processes to adapt to starvation in the endocrine and metabolic systems. We examined Sir2B function during early Dictyostelium development upon starvation. GFP-tagged Sir2B was expressed and the immunoreactivity was detected a punctate pattern near cell surface. Sir2B mRNA was expressed in vegetative cells and peaked at 2 h after starvation. Ectopic expression of Sir2B enhanced aggregate formation with increased expressions of cell adhesion molecules such as cadA, csaA, TgrB1, and TgrC1 at 8h compared to those in control cells. Sir2B null-mutant cells (Sir2B KO cells) were generated and they formed aggregates smaller than those of wild-type cells after 24 h. Because cell-cell adhesion affects aggregation size, cell cohesion assay of Sir2B KO cells showed cell-cell adhesion impairment at 8 and 10 h during development, and the expressions of four cell adhesion molecules were reduced in Sir2B KO cells compared to wild-type cells until 8 h after starvation. When RNAi-mediated cadA or csaA knockdown cells (cadA KD or csaA KD cells) were generated, csaA KD cells formed smaller aggregates and affected the expressions of other cell adhesion molecules to reduce, but cadA KD cells did not show to affect aggregation size and cell adhesion molecules expressions apparently. These results suggest that Sir2B is involved in csaA expression and affects other cell adhesion molecules during early development upon starvation.
VL  - 9
IS  - 1
ER  -

Copy | Download