Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment

Zeynu Shamil Awol; Rezika Tofike Abate

doi:doi:10.11648/j.cbe.20210602.12

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Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment

Zeynu Shamil Awol, Rezika Tofike Abate

Published in Chemical and Biomolecular Engineering (Volume 6, Issue 2)

Received: 29 April 2021 Accepted: 10 July 2021 Published: 21 July 2021

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Abstract

Water contamination was a serious problem throughout the world. Various technologically advanced treatment methodologies for example activated sludge process, membrane bioreactor, trickling filter, rotating biological contractor and oxidation ditch are widely studied well documented and adopted in practice. However, attention to promising low-investment-cost technologies such as slow biological waste water treatments after preliminary/primary treatment techniques was quite effective water treatment technology. It is also efficient in removing coli form microorganisms such as Cryptosporidium, E. coli and bacteriophage from wastewater. Apart from reduction of pathogenic load which is ascribed to the biological processes. This way of technology adopted the methodology taking the sample in the field of water surface two parts in sampling pond water in the surface pond water and inner parts also handling two different glass bottles. Then in analysis their analysis the sample subjected to physiochemical analysis then again the sample subjected to serial dilution to identify the microbe’s spp in the water sample (surface and deepest) part of pond water surface part of sample more microbes familiar conducted in this experiment. Then by using those identified microbes dosing this is done using 10^-6 and 10^-5 by prepared nutrient agar (3.25 gram with 250 ml of distilled water). Then take 1% (0.5), 3% (1.5) and 5% (2.5) by volume of broth added into waste water of 49.5 ml, 48.5 ml and 47.5 ml respectively. This means that for 50 ml of conical flask as the colonies of culture increase the probability of waste water to be clear was too been high so 47.5 with 5% (2.5) combined form of broth high potent of cleaned.

Published in	Chemical and Biomolecular Engineering (Volume 6, Issue 2)
DOI	10.11648/j.cbe.20210602.12
Page(s)	37-42
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Physiochemical Analysis, Biological Waste Water Treatment, Microbial Analysis, Pond Water, Nutrient Agar

References

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[4]	Camargo MCR, Toledo MCF Caffeine content of commercial Brazilian coffee Cienc Technol Aliment. 1998; 18: 421-4.
[5]	Dash SS, Gummadi SN. Biodegradation of caffeine by Pseudomonas sp. NCIM 5235, Research Journal of Microbiology. 2010; 5 (8): 745-753.
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[7]	Europaisches A. In: Coffeinum Theophyllinum, Deutscher Apotheker Verlag, Stuttgart; 1978, 670-121.
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[9]	Mussatto, S. I., & Roberto, I. C. (2005). Acid hydrolysis and fermentation of brewer‟s spent grain to produce xylitol. Journal of the Science of Food and Agriculture, 85, 2453–2460.
[10]	Mussatto, S. I., & Teixeira, J. A. (2010). Increase in the fructooligosaccharides yield and productivity by solid-state fermentation with Aspergillus japonicus using agro-industrial residues as support and nutrient source. Biochemical Engineering Journal, 53, 154–157.
[11]	Punj S, editors. Abiotic stress: new research. Hauppauge, NY: Nova science publishers Inc: 2012. pp. 1-57.
[12]	Rawel, H. M., & Kulling, S. E. (2007). Nutritional contribution of coffee, cacao and tea phenolics to human health. Journal of Consumer Protection and Food Safety, 2, 399–406.
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[15]	Salihu Ibrahim, Mohd Yunus Shukor, Mohd Arif Syed, Nor Arina Ab Rahman, Khalilah Abdul Khalil, Ariff Khalid et al. Bacterial degradation of caffeine: a Review. Asian Journal of Plant Biology. 2014; 2 (1): 18-27.
[16]	S. Clark and R. Pitt, “Storm water Runoff Treatment: Evaluation of Filtration Media,” US Environmental Protection Agency, Water Supply and Water Resources Division, National Risk Management Research Laboratory, EPA No. 600R00010, Cincinnati, 1999.
[17]	Nutrient management system. Can J Microbiol. 2008: 54; 876-886. doi: 10. 1139/W08-081. [PubMed] [Cross_Ref].
[18]	International Organization for Standardization (ISO) Standard 9000: 2005. Quality management system-fundamental and vocabulary. ISO, Switzerland, pp 1.
[19]	https://en.wikipedia.org/wiki/Bacteria
[20]	http://www.countyofdane.com/lwrd/landconservation/papers/impactsofswinfiltration.pdf
[21]	Wang, D., Sakoda, A., & Suzuki, M. (2001). Biological efficiency and nutritional value of Pleurotus ostreatus cultivated on spent beer grain. Bioresource Technology, 78, 293–300.
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APA Style

Zeynu Shamil Awol, Rezika Tofike Abate. (2021). Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment. Chemical and Biomolecular Engineering, 6(2), 37-42. https://doi.org/10.11648/j.cbe.20210602.12

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ACS Style

Zeynu Shamil Awol; Rezika Tofike Abate. Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment. Chem. Biomol. Eng. 2021, 6(2), 37-42. doi: 10.11648/j.cbe.20210602.12

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AMA Style

Zeynu Shamil Awol, Rezika Tofike Abate. Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment. Chem Biomol Eng. 2021;6(2):37-42. doi: 10.11648/j.cbe.20210602.12

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@article{10.11648/j.cbe.20210602.12,
  author = {Zeynu Shamil Awol and Rezika Tofike Abate},
  title = {Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment},
  journal = {Chemical and Biomolecular Engineering},
  volume = {6},
  number = {2},
  pages = {37-42},
  doi = {10.11648/j.cbe.20210602.12},
  url = {https://doi.org/10.11648/j.cbe.20210602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20210602.12},
  abstract = {Water contamination was a serious problem throughout the world. Various technologically advanced treatment methodologies for example activated sludge process, membrane bioreactor, trickling filter, rotating biological contractor and oxidation ditch are widely studied well documented and adopted in practice. However, attention to promising low-investment-cost technologies such as slow biological waste water treatments after preliminary/primary treatment techniques was quite effective water treatment technology. It is also efficient in removing coli form microorganisms such as Cryptosporidium, E. coli and bacteriophage from wastewater. Apart from reduction of pathogenic load which is ascribed to the biological processes. This way of technology adopted the methodology taking the sample in the field of water surface two parts in sampling pond water in the surface pond water and inner parts also handling two different glass bottles. Then in analysis their analysis the sample subjected to physiochemical analysis then again the sample subjected to serial dilution to identify the microbe’s spp in the water sample (surface and deepest) part of pond water surface part of sample more microbes familiar conducted in this experiment. Then by using those identified microbes dosing this is done using 10-6 and 10-5 by prepared nutrient agar (3.25 gram with 250 ml of distilled water). Then take 1% (0.5), 3% (1.5) and 5% (2.5) by volume of broth added into waste water of 49.5 ml, 48.5 ml and 47.5 ml respectively. This means that for 50 ml of conical flask as the colonies of culture increase the probability of waste water to be clear was too been high so 47.5 with 5% (2.5) combined form of broth high potent of cleaned.},
 year = {2021}
}

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TY  - JOUR
T1  - Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment
AU  - Zeynu Shamil Awol
AU  - Rezika Tofike Abate
Y1  - 2021/07/21
PY  - 2021
N1  - https://doi.org/10.11648/j.cbe.20210602.12
DO  - 10.11648/j.cbe.20210602.12
T2  - Chemical and Biomolecular Engineering
JF  - Chemical and Biomolecular Engineering
JO  - Chemical and Biomolecular Engineering
SP  - 37
EP  - 42
PB  - Science Publishing Group
SN  - 2578-8884
UR  - https://doi.org/10.11648/j.cbe.20210602.12
AB  - Water contamination was a serious problem throughout the world. Various technologically advanced treatment methodologies for example activated sludge process, membrane bioreactor, trickling filter, rotating biological contractor and oxidation ditch are widely studied well documented and adopted in practice. However, attention to promising low-investment-cost technologies such as slow biological waste water treatments after preliminary/primary treatment techniques was quite effective water treatment technology. It is also efficient in removing coli form microorganisms such as Cryptosporidium, E. coli and bacteriophage from wastewater. Apart from reduction of pathogenic load which is ascribed to the biological processes. This way of technology adopted the methodology taking the sample in the field of water surface two parts in sampling pond water in the surface pond water and inner parts also handling two different glass bottles. Then in analysis their analysis the sample subjected to physiochemical analysis then again the sample subjected to serial dilution to identify the microbe’s spp in the water sample (surface and deepest) part of pond water surface part of sample more microbes familiar conducted in this experiment. Then by using those identified microbes dosing this is done using 10-6 and 10-5 by prepared nutrient agar (3.25 gram with 250 ml of distilled water). Then take 1% (0.5), 3% (1.5) and 5% (2.5) by volume of broth added into waste water of 49.5 ml, 48.5 ml and 47.5 ml respectively. This means that for 50 ml of conical flask as the colonies of culture increase the probability of waste water to be clear was too been high so 47.5 with 5% (2.5) combined form of broth high potent of cleaned.
VL  - 6
IS  - 2
ER  -

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Author Information

Zeynu Shamil Awol

Department of Chemical Engineering, College of Engineering and Technology, Wolkite University, Wolkite, Ethiopian
Rezika Tofike Abate

Department of Chemical Engineering, College of Engineering and Technology, Wolkite University, Wolkite, Ethiopian

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APA Style

Zeynu Shamil Awol, Rezika Tofike Abate. (2021). Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment. Chemical and Biomolecular Engineering, 6(2), 37-42. https://doi.org/10.11648/j.cbe.20210602.12

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ACS Style

Zeynu Shamil Awol; Rezika Tofike Abate. Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment. Chem. Biomol. Eng. 2021, 6(2), 37-42. doi: 10.11648/j.cbe.20210602.12

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AMA Style

Zeynu Shamil Awol, Rezika Tofike Abate. Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment. Chem Biomol Eng. 2021;6(2):37-42. doi: 10.11648/j.cbe.20210602.12

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@article{10.11648/j.cbe.20210602.12,
  author = {Zeynu Shamil Awol and Rezika Tofike Abate},
  title = {Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment},
  journal = {Chemical and Biomolecular Engineering},
  volume = {6},
  number = {2},
  pages = {37-42},
  doi = {10.11648/j.cbe.20210602.12},
  url = {https://doi.org/10.11648/j.cbe.20210602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20210602.12},
  abstract = {Water contamination was a serious problem throughout the world. Various technologically advanced treatment methodologies for example activated sludge process, membrane bioreactor, trickling filter, rotating biological contractor and oxidation ditch are widely studied well documented and adopted in practice. However, attention to promising low-investment-cost technologies such as slow biological waste water treatments after preliminary/primary treatment techniques was quite effective water treatment technology. It is also efficient in removing coli form microorganisms such as Cryptosporidium, E. coli and bacteriophage from wastewater. Apart from reduction of pathogenic load which is ascribed to the biological processes. This way of technology adopted the methodology taking the sample in the field of water surface two parts in sampling pond water in the surface pond water and inner parts also handling two different glass bottles. Then in analysis their analysis the sample subjected to physiochemical analysis then again the sample subjected to serial dilution to identify the microbe’s spp in the water sample (surface and deepest) part of pond water surface part of sample more microbes familiar conducted in this experiment. Then by using those identified microbes dosing this is done using 10-6 and 10-5 by prepared nutrient agar (3.25 gram with 250 ml of distilled water). Then take 1% (0.5), 3% (1.5) and 5% (2.5) by volume of broth added into waste water of 49.5 ml, 48.5 ml and 47.5 ml respectively. This means that for 50 ml of conical flask as the colonies of culture increase the probability of waste water to be clear was too been high so 47.5 with 5% (2.5) combined form of broth high potent of cleaned.},
 year = {2021}
}

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TY  - JOUR
T1  - Analysis of Microbe's from Municipal Waste Water (Pond) and Uses the Microbes for the Application of Waste Water Treatment
AU  - Zeynu Shamil Awol
AU  - Rezika Tofike Abate
Y1  - 2021/07/21
PY  - 2021
N1  - https://doi.org/10.11648/j.cbe.20210602.12
DO  - 10.11648/j.cbe.20210602.12
T2  - Chemical and Biomolecular Engineering
JF  - Chemical and Biomolecular Engineering
JO  - Chemical and Biomolecular Engineering
SP  - 37
EP  - 42
PB  - Science Publishing Group
SN  - 2578-8884
UR  - https://doi.org/10.11648/j.cbe.20210602.12
AB  - Water contamination was a serious problem throughout the world. Various technologically advanced treatment methodologies for example activated sludge process, membrane bioreactor, trickling filter, rotating biological contractor and oxidation ditch are widely studied well documented and adopted in practice. However, attention to promising low-investment-cost technologies such as slow biological waste water treatments after preliminary/primary treatment techniques was quite effective water treatment technology. It is also efficient in removing coli form microorganisms such as Cryptosporidium, E. coli and bacteriophage from wastewater. Apart from reduction of pathogenic load which is ascribed to the biological processes. This way of technology adopted the methodology taking the sample in the field of water surface two parts in sampling pond water in the surface pond water and inner parts also handling two different glass bottles. Then in analysis their analysis the sample subjected to physiochemical analysis then again the sample subjected to serial dilution to identify the microbe’s spp in the water sample (surface and deepest) part of pond water surface part of sample more microbes familiar conducted in this experiment. Then by using those identified microbes dosing this is done using 10-6 and 10-5 by prepared nutrient agar (3.25 gram with 250 ml of distilled water). Then take 1% (0.5), 3% (1.5) and 5% (2.5) by volume of broth added into waste water of 49.5 ml, 48.5 ml and 47.5 ml respectively. This means that for 50 ml of conical flask as the colonies of culture increase the probability of waste water to be clear was too been high so 47.5 with 5% (2.5) combined form of broth high potent of cleaned.
VL  - 6
IS  - 2
ER  -

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