Antibiotic Resistance Pattern of Clinical Isolates - Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus in the Western of Bangladesh

Mohammad Golam Sakline; Dabashis Roy; Tanvir Ahmad; Shaikh Shahinoor Rahman; Shakh Mohammad Abdur Rouf; Mosammat Nazira Akhter Rithu; Mohammad Mizanur Rahman; Mohammad Tanvir Sarwar

doi:doi:10.11648/j.fem.20210702.14

Antibiotic Resistance Pattern of Clinical Isolates - Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus in the Western of Bangladesh

Mohammad Golam Sakline, Dabashis Roy, Tanvir Ahmad, Shaikh Shahinoor Rahman, Shakh Mohammad Abdur Rouf, Mosammat Nazira Akhter Rithu, Mohammad Mizanur Rahman, Mohammad Tanvir Sarwar

Published in Frontiers in Environmental Microbiology (Volume 7, Issue 2)

Received: 26 March 2021 Accepted: 20 May 2021 Published: 31 May 2021

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Abstract

The present study was carried out to analyze the antibiotic susceptibility of four pathogenic bacteria Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus respectively. Escherichia coli strains from 35 samples, Staphylococcusaureus strains from 35 samples, Enterobacter strains from 39 samples and Pseudomonas strains from 39 samples were isolated from 200 suspected infected individuals. Pure cultures of isolate were done by isolating single colony from the stored bacteria. Identification of strains were confirmed by various microscopic, colonial and biochemical tests. Finally identified four varieties of pathogenic strains Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus were subjected to the antibiotic sensitivity test by antibiotic disc diffusion method. Approximately 15 commonly used antibiotics were used in the tests. For this study it was observed that 94% of Escherichia coli were resistant to Cefixime, 86% to Cefuroxime Sodium, 77% to Ceftriaxone, 71% to Ceftazidime, 66% to Amoxycillin-Clavulanic acid and Ciprofloxacin, 63% to Levofloxacin, 57% to Doxycycline, 49% to Co-trimoxazole andonly 37% to Gentamicin. No Escherichia coli samples were found resistant against Meropenem having highest sensitivity (100%). Only 7 Escherichia coli samples were resistant to Amikacin having sensitivity 80% and 10 Escherichia coli samples were resistant to Nitrofurantion with the third highest sensitivity 71%. Staphylococcus aureus were observed to show maximum resistant (100%) towards Azithromycin, next to Ceftriaxone 74%, Ciprofloxacin and Oxacillin 71%, Co-trimoxazole 63%, Levofloxacin 57% and Amoxycillin-Clavulanic acid 49%. Only 5 Staphylococcus aureus samples were resistant to Gentamicin with a maximum sensitivity 86% and 7 Staphylococcus aureus samples were resistant to Amikacin having second highest sensitivity 80% and third highest sensitivity Cephalexin 63%. Enterobactor showedmaximum resistant towards Amoxycillin-Clavunic acid 100%, Cefixime 100%, Ceftadizime 100%, Ceftriaxone 95%, Amikacin 85%, Co-trimoxazole 79%, Ciprofloxacine 77%, Doxycyclin 72%, Gentamicin51%, Levofloxacin 46%, and Nitrofurantion 41%. There were found to be no Enterobacter Samples that resistant to Meropenem having highest sensitivity (100%). Only 16 Entarobacter samples were resistant to Nitrofurantion having second highest sensitivity59% and third highest sensitivity Levofloxacin 54%. Pseudomonus were observed to maximum resistant towards Amoxicillin 100%, Ceftazidime 100%, Cefixime 100%, Doxycycline 100%, Co-trimoxazole 100%, Ciprofloxacin 83%, Ceftriaxone 83%, Levofloxacin 83%, Nitrofurantion 83%, and Amikacin 67%. There were found to be no Pseudomonus Samples that resistant to Meropenem having highest sensitivity (100%). Only 13 Pseudomonus samples were resistant to Gentamicin with a maximumsensitivity 67%.

Published in	Frontiers in Environmental Microbiology (Volume 7, Issue 2)
DOI	10.11648/j.fem.20210702.14
Page(s)	69-73
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Resistant Microbes, Escherichia Coli, Enterobacter, Pseudomonas and Staphylococcus Aureus

References

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[2]	Okeke IN, Lamikanra A, Edelman R. Socioeconomic and behavioral factors leading to acquired bacterial resistance to antibiotics in developing countries. Emerg Infect Dis. 1999; 5: 18–27. World Health Organization. Overcoming antimicrobial resistance. Geneva, WHO, 2000. Faiz MA, Basher A. Antimicrobial resistance: Bangladesh experience. Regional Health Forum 2011; 15: 1-8.
[3]	Rahman MS, Khan IA, Chowdhury S, Islam AMZ, Sultana R, Hoque MZ, Akhter N. A baseline survey on the use of drugs at private practitioner level in Bangladesh. Bangladesh J Physiol Pharmacol. 1998; 14: 47-50.
[4]	Asna S, Haq JA, Rahman MM. Nalidixic acid-resistant Salmonella enterica serovar Typhi with decreased susceptibility to ciprofloxacin caused treatment failure: a report from Bangladesh. Jpn J Infect Dis 2003; 56 (1): 32–3.
[5]	Biswas M, Roy DN, Tajmim A, Rajib SS, Hossain M, Farzana F, et al. Prescription antibiotics for outpatients in Bangladesh: a cross-sectional health survey conducted in three cities. Ann Clin Microbiol Antimicrob 2014a; 13 (1): 15.
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[7]	Sutradhar KB, Saha A, Huda NH, Uddin R. Irrational use of antibiotics and antibiotic resistance in southern rural Bangladesh: perspectives from both the physicians and patients. Annu Res Rev Biol 2014; 4 (9): 1421–30.
[8]	Islam MS, Rahman MS, Misbahuddin M. Impact of ‘Prescription Audit & Feedback’ on pattern of prophylactic antimicrobials in caesarean section: A cost reduction perspective. Bangladesh J Physiol Pharmacol. 2007; 23: 1-9.
[9]	Chowdhury AK, Rahman MS, Faroque AB, Hasan GA, Raihan SZ. Excessive use of avoidable therapeutic injections in the Upazilla health complexes of Bangladesh. Mymensingh Med J. 2008; 17 (2 Suppl): 59-64.
[10]	Das AK, Rahman MS. Prescribing vitamins at primary health care level: Exploration of facts, factors and solution. Bangladesh J Pharmacol. 2010; 5: 92-97.
[11]	Holloway KA. Bangladesh: Pharmaceuticals in health care delivery. Mission Report. New Delhi, W orld Health Organization, Regional Office for South East Asia, 2010; 17-24.
[12]	Buchanan RE, Gibbons NE (eds.). Bergey’s manual of determinative Bacteriology. 8th ed. Baltimore: The Williams and Wilkins. 1974.
[13]	Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 1966; 45 (4): 493–496.
[14]	S. C. Davies, T. Fowler, J. Watson, D. M. Livermore, and D. Walker, “Annual report of the chief medical officer: infection and the rise of antimicrobial resistance,” The Lancet, 2013; 381 (9878): 1606–1609.
[15]	World Health Organization, The Evolving Threat of Antimicrobial Resistance: Options for Action, World Health Organization, Geneva, 2012.
[16]	Centres for Disease Control and Prevention (US), Antibiotic resistance threats in the United States, 2013, Centres for Disease Control and Prevention, US Department of Health and Human Services, 2013.
[17]	S. B. Levy and B. Marshall, “Antibacterial resistance worldwide: causes, challenges and responses,” Nature Medicine, 2004; 10 (12): 122–129.
[18]	A. Tsutsui and S. Suzuki, “Japan nosocomial infections surveillance (JANIS): a model of sustainable national antimicrobial resistance surveillance based on hospital diagnostic microbiology laboratories,” BMC Health Services Research, 2018; 18 (1): 799.
[19]	Organization WH, “Global action plan on antimicrobial resistance,” https://www.who.int/antimicrobial-resistance/globalaction-plan/en/.
[20]	Organization WH, “Global Antimicrobial Resistance Surveillance System (GLASS) Report: Early Implementation 2017-2018,” https://www.who.int/drugresistance/surveillance/ GLASSmeeting/en/.

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Mohammad Golam Sakline, Dabashis Roy, Tanvir Ahmad, Shaikh Shahinoor Rahman, Shakh Mohammad Abdur Rouf, et al. (2021). Antibiotic Resistance Pattern of Clinical Isolates - Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus in the Western of Bangladesh. Frontiers in Environmental Microbiology, 7(2), 69-73. https://doi.org/10.11648/j.fem.20210702.14

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Mohammad Golam Sakline; Dabashis Roy; Tanvir Ahmad; Shaikh Shahinoor Rahman; Shakh Mohammad Abdur Rouf, et al. Antibiotic Resistance Pattern of Clinical Isolates - Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus in the Western of Bangladesh. Front. Environ. Microbiol. 2021, 7(2), 69-73. doi: 10.11648/j.fem.20210702.14

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AMA Style

Mohammad Golam Sakline, Dabashis Roy, Tanvir Ahmad, Shaikh Shahinoor Rahman, Shakh Mohammad Abdur Rouf, et al. Antibiotic Resistance Pattern of Clinical Isolates - Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus in the Western of Bangladesh. Front Environ Microbiol. 2021;7(2):69-73. doi: 10.11648/j.fem.20210702.14

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@article{10.11648/j.fem.20210702.14,
  author = {Mohammad Golam Sakline and Dabashis Roy and Tanvir Ahmad and Shaikh Shahinoor Rahman and Shakh Mohammad Abdur Rouf and Mosammat Nazira Akhter Rithu and Mohammad Mizanur Rahman and Mohammad Tanvir Sarwar},
  title = {Antibiotic Resistance Pattern of Clinical Isolates - Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus in the Western of Bangladesh},
  journal = {Frontiers in Environmental Microbiology},
  volume = {7},
  number = {2},
  pages = {69-73},
  doi = {10.11648/j.fem.20210702.14},
  url = {https://doi.org/10.11648/j.fem.20210702.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20210702.14},
  abstract = {The present study was carried out to analyze the antibiotic susceptibility of four pathogenic bacteria Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus respectively. Escherichia coli strains from 35 samples, Staphylococcusaureus strains from 35 samples, Enterobacter strains from 39 samples and Pseudomonas strains from 39 samples were isolated from 200 suspected infected individuals. Pure cultures of isolate were done by isolating single colony from the stored bacteria. Identification of strains were confirmed by various microscopic, colonial and biochemical tests. Finally identified four varieties of pathogenic strains Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus were subjected to the antibiotic sensitivity test by antibiotic disc diffusion method. Approximately 15 commonly used antibiotics were used in the tests. For this study it was observed that 94% of Escherichia coli were resistant to Cefixime, 86% to Cefuroxime Sodium, 77% to Ceftriaxone, 71% to Ceftazidime, 66% to Amoxycillin-Clavulanic acid and Ciprofloxacin, 63% to Levofloxacin, 57% to Doxycycline, 49% to Co-trimoxazole andonly 37% to Gentamicin. No Escherichia coli samples were found resistant against Meropenem having highest sensitivity (100%). Only 7 Escherichia coli samples were resistant to Amikacin having sensitivity 80% and 10 Escherichia coli samples were resistant to Nitrofurantion with the third highest sensitivity 71%. Staphylococcus aureus were observed to show maximum resistant (100%) towards Azithromycin, next to Ceftriaxone 74%, Ciprofloxacin and Oxacillin 71%, Co-trimoxazole 63%, Levofloxacin 57% and Amoxycillin-Clavulanic acid 49%. Only 5 Staphylococcus aureus samples were resistant to Gentamicin with a maximum sensitivity 86% and 7 Staphylococcus aureus samples were resistant to Amikacin having second highest sensitivity 80% and third highest sensitivity Cephalexin 63%. Enterobactor showedmaximum resistant towards Amoxycillin-Clavunic acid 100%, Cefixime 100%, Ceftadizime 100%, Ceftriaxone 95%, Amikacin 85%, Co-trimoxazole 79%, Ciprofloxacine 77%, Doxycyclin 72%, Gentamicin51%, Levofloxacin 46%, and Nitrofurantion 41%. There were found to be no Enterobacter Samples that resistant to Meropenem having highest sensitivity (100%). Only 16 Entarobacter samples were resistant to Nitrofurantion having second highest sensitivity59% and third highest sensitivity Levofloxacin 54%. Pseudomonus were observed to maximum resistant towards Amoxicillin 100%, Ceftazidime 100%, Cefixime 100%, Doxycycline 100%, Co-trimoxazole 100%, Ciprofloxacin 83%, Ceftriaxone 83%, Levofloxacin 83%, Nitrofurantion 83%, and Amikacin 67%. There were found to be no Pseudomonus Samples that resistant to Meropenem having highest sensitivity (100%). Only 13 Pseudomonus samples were resistant to Gentamicin with a maximumsensitivity 67%.},
 year = {2021}
}

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TY  - JOUR
T1  - Antibiotic Resistance Pattern of Clinical Isolates - Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus in the Western of Bangladesh
AU  - Mohammad Golam Sakline
AU  - Dabashis Roy
AU  - Tanvir Ahmad
AU  - Shaikh Shahinoor Rahman
AU  - Shakh Mohammad Abdur Rouf
AU  - Mosammat Nazira Akhter Rithu
AU  - Mohammad Mizanur Rahman
AU  - Mohammad Tanvir Sarwar
Y1  - 2021/05/31
PY  - 2021
N1  - https://doi.org/10.11648/j.fem.20210702.14
DO  - 10.11648/j.fem.20210702.14
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 69
EP  - 73
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20210702.14
AB  - The present study was carried out to analyze the antibiotic susceptibility of four pathogenic bacteria Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus respectively. Escherichia coli strains from 35 samples, Staphylococcusaureus strains from 35 samples, Enterobacter strains from 39 samples and Pseudomonas strains from 39 samples were isolated from 200 suspected infected individuals. Pure cultures of isolate were done by isolating single colony from the stored bacteria. Identification of strains were confirmed by various microscopic, colonial and biochemical tests. Finally identified four varieties of pathogenic strains Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus were subjected to the antibiotic sensitivity test by antibiotic disc diffusion method. Approximately 15 commonly used antibiotics were used in the tests. For this study it was observed that 94% of Escherichia coli were resistant to Cefixime, 86% to Cefuroxime Sodium, 77% to Ceftriaxone, 71% to Ceftazidime, 66% to Amoxycillin-Clavulanic acid and Ciprofloxacin, 63% to Levofloxacin, 57% to Doxycycline, 49% to Co-trimoxazole andonly 37% to Gentamicin. No Escherichia coli samples were found resistant against Meropenem having highest sensitivity (100%). Only 7 Escherichia coli samples were resistant to Amikacin having sensitivity 80% and 10 Escherichia coli samples were resistant to Nitrofurantion with the third highest sensitivity 71%. Staphylococcus aureus were observed to show maximum resistant (100%) towards Azithromycin, next to Ceftriaxone 74%, Ciprofloxacin and Oxacillin 71%, Co-trimoxazole 63%, Levofloxacin 57% and Amoxycillin-Clavulanic acid 49%. Only 5 Staphylococcus aureus samples were resistant to Gentamicin with a maximum sensitivity 86% and 7 Staphylococcus aureus samples were resistant to Amikacin having second highest sensitivity 80% and third highest sensitivity Cephalexin 63%. Enterobactor showedmaximum resistant towards Amoxycillin-Clavunic acid 100%, Cefixime 100%, Ceftadizime 100%, Ceftriaxone 95%, Amikacin 85%, Co-trimoxazole 79%, Ciprofloxacine 77%, Doxycyclin 72%, Gentamicin51%, Levofloxacin 46%, and Nitrofurantion 41%. There were found to be no Enterobacter Samples that resistant to Meropenem having highest sensitivity (100%). Only 16 Entarobacter samples were resistant to Nitrofurantion having second highest sensitivity59% and third highest sensitivity Levofloxacin 54%. Pseudomonus were observed to maximum resistant towards Amoxicillin 100%, Ceftazidime 100%, Cefixime 100%, Doxycycline 100%, Co-trimoxazole 100%, Ciprofloxacin 83%, Ceftriaxone 83%, Levofloxacin 83%, Nitrofurantion 83%, and Amikacin 67%. There were found to be no Pseudomonus Samples that resistant to Meropenem having highest sensitivity (100%). Only 13 Pseudomonus samples were resistant to Gentamicin with a maximumsensitivity 67%.
VL  - 7
IS  - 2
ER  -

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Author Information

Mohammad Golam Sakline

Department of Applied Nutrition and Food Technology, Faculty of Biological Sciences, Islamic University Kushtia, Kushtia, Bangladesh
Dabashis Roy

Department of Applied Nutrition and Food Technology, Faculty of Biological Sciences, Islamic University Kushtia, Kushtia, Bangladesh
Tanvir Ahmad

Department of Nutrition and Food Technology, Jashore University of Science and Technology, Jashore, Bangladesh
Shaikh Shahinoor Rahman

Department of Applied Nutrition and Food Technology, Faculty of Biological Sciences, Islamic University Kushtia, Kushtia, Bangladesh
Shakh Mohammad Abdur Rouf

Department of Applied Nutrition and Food Technology, Faculty of Biological Sciences, Islamic University Kushtia, Kushtia, Bangladesh
Mosammat Nazira Akhter Rithu

Department of Geography and Environmental Studies, University of Rajshahi, Rajshahi, Bangladesh
Mohammad Mizanur Rahman

Department of Fisheries Technology, Patuakhali Science and Technology University, Patuakhali, Bangladesh
Mohammad Tanvir Sarwar

Department of Applied Nutrition and Food Technology, Faculty of Biological Sciences, Islamic University Kushtia, Kushtia, Bangladesh

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APA Style

Mohammad Golam Sakline, Dabashis Roy, Tanvir Ahmad, Shaikh Shahinoor Rahman, Shakh Mohammad Abdur Rouf, et al. (2021). Antibiotic Resistance Pattern of Clinical Isolates - Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus in the Western of Bangladesh. Frontiers in Environmental Microbiology, 7(2), 69-73. https://doi.org/10.11648/j.fem.20210702.14

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ACS Style

Mohammad Golam Sakline; Dabashis Roy; Tanvir Ahmad; Shaikh Shahinoor Rahman; Shakh Mohammad Abdur Rouf, et al. Antibiotic Resistance Pattern of Clinical Isolates - Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus in the Western of Bangladesh. Front. Environ. Microbiol. 2021, 7(2), 69-73. doi: 10.11648/j.fem.20210702.14

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AMA Style

Mohammad Golam Sakline, Dabashis Roy, Tanvir Ahmad, Shaikh Shahinoor Rahman, Shakh Mohammad Abdur Rouf, et al. Antibiotic Resistance Pattern of Clinical Isolates - Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus in the Western of Bangladesh. Front Environ Microbiol. 2021;7(2):69-73. doi: 10.11648/j.fem.20210702.14

Copy | Download

@article{10.11648/j.fem.20210702.14,
  author = {Mohammad Golam Sakline and Dabashis Roy and Tanvir Ahmad and Shaikh Shahinoor Rahman and Shakh Mohammad Abdur Rouf and Mosammat Nazira Akhter Rithu and Mohammad Mizanur Rahman and Mohammad Tanvir Sarwar},
  title = {Antibiotic Resistance Pattern of Clinical Isolates - Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus in the Western of Bangladesh},
  journal = {Frontiers in Environmental Microbiology},
  volume = {7},
  number = {2},
  pages = {69-73},
  doi = {10.11648/j.fem.20210702.14},
  url = {https://doi.org/10.11648/j.fem.20210702.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20210702.14},
  abstract = {The present study was carried out to analyze the antibiotic susceptibility of four pathogenic bacteria Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus respectively. Escherichia coli strains from 35 samples, Staphylococcusaureus strains from 35 samples, Enterobacter strains from 39 samples and Pseudomonas strains from 39 samples were isolated from 200 suspected infected individuals. Pure cultures of isolate were done by isolating single colony from the stored bacteria. Identification of strains were confirmed by various microscopic, colonial and biochemical tests. Finally identified four varieties of pathogenic strains Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus were subjected to the antibiotic sensitivity test by antibiotic disc diffusion method. Approximately 15 commonly used antibiotics were used in the tests. For this study it was observed that 94% of Escherichia coli were resistant to Cefixime, 86% to Cefuroxime Sodium, 77% to Ceftriaxone, 71% to Ceftazidime, 66% to Amoxycillin-Clavulanic acid and Ciprofloxacin, 63% to Levofloxacin, 57% to Doxycycline, 49% to Co-trimoxazole andonly 37% to Gentamicin. No Escherichia coli samples were found resistant against Meropenem having highest sensitivity (100%). Only 7 Escherichia coli samples were resistant to Amikacin having sensitivity 80% and 10 Escherichia coli samples were resistant to Nitrofurantion with the third highest sensitivity 71%. Staphylococcus aureus were observed to show maximum resistant (100%) towards Azithromycin, next to Ceftriaxone 74%, Ciprofloxacin and Oxacillin 71%, Co-trimoxazole 63%, Levofloxacin 57% and Amoxycillin-Clavulanic acid 49%. Only 5 Staphylococcus aureus samples were resistant to Gentamicin with a maximum sensitivity 86% and 7 Staphylococcus aureus samples were resistant to Amikacin having second highest sensitivity 80% and third highest sensitivity Cephalexin 63%. Enterobactor showedmaximum resistant towards Amoxycillin-Clavunic acid 100%, Cefixime 100%, Ceftadizime 100%, Ceftriaxone 95%, Amikacin 85%, Co-trimoxazole 79%, Ciprofloxacine 77%, Doxycyclin 72%, Gentamicin51%, Levofloxacin 46%, and Nitrofurantion 41%. There were found to be no Enterobacter Samples that resistant to Meropenem having highest sensitivity (100%). Only 16 Entarobacter samples were resistant to Nitrofurantion having second highest sensitivity59% and third highest sensitivity Levofloxacin 54%. Pseudomonus were observed to maximum resistant towards Amoxicillin 100%, Ceftazidime 100%, Cefixime 100%, Doxycycline 100%, Co-trimoxazole 100%, Ciprofloxacin 83%, Ceftriaxone 83%, Levofloxacin 83%, Nitrofurantion 83%, and Amikacin 67%. There were found to be no Pseudomonus Samples that resistant to Meropenem having highest sensitivity (100%). Only 13 Pseudomonus samples were resistant to Gentamicin with a maximumsensitivity 67%.},
 year = {2021}
}

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TY  - JOUR
T1  - Antibiotic Resistance Pattern of Clinical Isolates - Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus in the Western of Bangladesh
AU  - Mohammad Golam Sakline
AU  - Dabashis Roy
AU  - Tanvir Ahmad
AU  - Shaikh Shahinoor Rahman
AU  - Shakh Mohammad Abdur Rouf
AU  - Mosammat Nazira Akhter Rithu
AU  - Mohammad Mizanur Rahman
AU  - Mohammad Tanvir Sarwar
Y1  - 2021/05/31
PY  - 2021
N1  - https://doi.org/10.11648/j.fem.20210702.14
DO  - 10.11648/j.fem.20210702.14
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 69
EP  - 73
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20210702.14
AB  - The present study was carried out to analyze the antibiotic susceptibility of four pathogenic bacteria Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus respectively. Escherichia coli strains from 35 samples, Staphylococcusaureus strains from 35 samples, Enterobacter strains from 39 samples and Pseudomonas strains from 39 samples were isolated from 200 suspected infected individuals. Pure cultures of isolate were done by isolating single colony from the stored bacteria. Identification of strains were confirmed by various microscopic, colonial and biochemical tests. Finally identified four varieties of pathogenic strains Escherichia coli, Enterobacter, Pseudomonas and Staphylococcus aureus were subjected to the antibiotic sensitivity test by antibiotic disc diffusion method. Approximately 15 commonly used antibiotics were used in the tests. For this study it was observed that 94% of Escherichia coli were resistant to Cefixime, 86% to Cefuroxime Sodium, 77% to Ceftriaxone, 71% to Ceftazidime, 66% to Amoxycillin-Clavulanic acid and Ciprofloxacin, 63% to Levofloxacin, 57% to Doxycycline, 49% to Co-trimoxazole andonly 37% to Gentamicin. No Escherichia coli samples were found resistant against Meropenem having highest sensitivity (100%). Only 7 Escherichia coli samples were resistant to Amikacin having sensitivity 80% and 10 Escherichia coli samples were resistant to Nitrofurantion with the third highest sensitivity 71%. Staphylococcus aureus were observed to show maximum resistant (100%) towards Azithromycin, next to Ceftriaxone 74%, Ciprofloxacin and Oxacillin 71%, Co-trimoxazole 63%, Levofloxacin 57% and Amoxycillin-Clavulanic acid 49%. Only 5 Staphylococcus aureus samples were resistant to Gentamicin with a maximum sensitivity 86% and 7 Staphylococcus aureus samples were resistant to Amikacin having second highest sensitivity 80% and third highest sensitivity Cephalexin 63%. Enterobactor showedmaximum resistant towards Amoxycillin-Clavunic acid 100%, Cefixime 100%, Ceftadizime 100%, Ceftriaxone 95%, Amikacin 85%, Co-trimoxazole 79%, Ciprofloxacine 77%, Doxycyclin 72%, Gentamicin51%, Levofloxacin 46%, and Nitrofurantion 41%. There were found to be no Enterobacter Samples that resistant to Meropenem having highest sensitivity (100%). Only 16 Entarobacter samples were resistant to Nitrofurantion having second highest sensitivity59% and third highest sensitivity Levofloxacin 54%. Pseudomonus were observed to maximum resistant towards Amoxicillin 100%, Ceftazidime 100%, Cefixime 100%, Doxycycline 100%, Co-trimoxazole 100%, Ciprofloxacin 83%, Ceftriaxone 83%, Levofloxacin 83%, Nitrofurantion 83%, and Amikacin 67%. There were found to be no Pseudomonus Samples that resistant to Meropenem having highest sensitivity (100%). Only 13 Pseudomonus samples were resistant to Gentamicin with a maximumsensitivity 67%.
VL  - 7
IS  - 2
ER  -

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