Medicinal Plants are Plants used for traditional medicines. Any part of the plant could be used as medicine. Medicinal plants have been discovered and used in traditional medicine practices since pre-historical times. A survey of traditional medicine man in Damaturu indicated that Lannia microcarpa is a plants used in the treatment of inflammation, wounds and cancer. The fresh samples (stem bark) of the plant was collected and successively extracted with n-hexane, ethyl acetate, methanol and water. Phytochemical constituents of Lannea microcarpa extracts revealed the presence of cardiac glycosides, flavonoids, saponins, phenols, tannins, alkaloids, sterols and terpenoids. The antioxidant potential was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay, whereas membrane stabilization method was used to evaluate the anti-inflammatory potential of the extracts. The antibacterial potential of the plant extracts against four (4) wound pathogens: Salmonella typhi, Staphylococcus aureus, Escherichia coli and Strephtococcus pneumoneae, was evaluated using disc diffusion technician. The IC50 values obtained for the Lannea microcarpa extracts indicated that the methanol and water extracts exhibited higher antioxidant activities than the standard ascorbic acid. The extracts were found effective in inhibiting the heat induced haemolysis. Diclofenac Sodium salt used as standard drug at 100μg/ml offered 90.66% protection a significant (p<0.05) protection against damaging effect of heat solution. The results showed that all extracts from the plant offered a significant (p<0.05) protection against the damaging effect of hypotonic solution, Diclofenac sodium (100μg/ml), the standard drug offered a significant (p<0.05) protection (57.52%). Lannea microcarpa extracts showed zone of inhibition only against Salmonella typhi and Strephtococcus pneumonea.
| Published in | Journal of Diseases and Medicinal Plants (Volume 9, Issue 1) |
| DOI | 10.11648/j.jdmp.20230901.13 |
| Page(s) | 21-32 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Phytochemical, Antioxidant, Antibacterial, Anti-Inflammatory
| [1] | Baiano, A. and Del Nobile, M. A. (2015). Antioxidant Compounds from Vegetable Matrices: Biosynthesis, Occurrence and Extraction Systems. Critical Review in Food Science and Nutrition. 56 (12): 2053-68. |
| [2] | Baris, O., Gulluce, M., Sahin, F., Ozer, H., Ozkan, H., Sokmen, M. and Ozbek, T. (2006). Biological activities of essential oil and methanol extract of Achillea Biebersteinii Afan. (Asteraceae). Turkish Journal of Biology. 30: 65-73. |
| [3] | Cai, Y., Luo, Q., Sun, M. and Corke, H. (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sciences. 74 (17): 2157-84. |
| [4] | Cheesbrough, M. (2002). Biochemical Test to Identify Bacteria. In: Laboratory Practice in Tropical Countries. Cambridge Edition. Pp. 63-70. |
| [5] | Fang, Y. Z., Yang, S. and Wu, G. (2014). Free radicals, antioxidants and nutrition. Nutrition. 18: 872-879. |
| [6] | Fawole, A. M. (2009). Pharmacological and Phytochemistry of South African Traditional Medicinal plants used as antimicrobials. M.sc Thesis. University of Kwazulu Natal, Pietermeritzburg. |
| [7] | Gandhidasan, R., Thamaraichelvan, A. and Baburaj, S. (1991). Anti-inflammatory action of Lannea coromandelica by HRBC membrane stabilization. Fitoterapia. 12 (1): 81-83. |
| [8] | Gupta, M., Mazumdr, U. K., Sivakumar, T., Vamis M. L., Karkis, S., Sambathkumar, R. and Mainkndn, L. (2003). antioxidant and Anti-inflammatory activities of Acalypha fructicasa. Nig. J. Nat. Prd. Med. 25-29. |
| [9] | Hammer, K. A., Carson, C. F. and Riley, T. V. (1999). Antimicrobial activity of essential oils and other plants extracts. J. Appl. Microbiol. 86 (6): 985. |
| [10] | Hatano, T., Kagw, H., Yasuhara, T., and Okud, T., (1988). Two new flavonoid and other constituents in licorice root: their relative astringent and radical scavenging effects. Chem. Pharm. Bull. 36: 1090-2097. |
| [11] | Khaleeliah, W. M. H (2001). Screening of anti-cancer activity of Palestinian plants. M.sc Thesis, An Najah National University, Palestine, pp: 1-10. |
| [12] | Md. Lolo, C. M. (2009). Phytochemical analysis and selected biological activity of phyllanthus parvulus sond. Var. garipensis. M.sc thesis. University of Zululand, South Africa. |
| [13] | Okafor, T. and Mukhtar, M. D. (2002). Antibacterial Activity of Ethanolic Extract of Guiera senegalensis. International Journal of Pharmacology; 56: 213-216. |
| [14] | Okwu, D. E. (2004) Evaluation of the chemical composition of Indigenous species and flavoring agents. Global Journal of Pure and Applied Science 7 (3): 455-459. |
| [15] | Okwu, D. E. and Ekeke, O. (2003). Phytochemical Screening and mineral composition of chewing stick in south – eastern Nigeria. Global Journal of Pure and Applied Sciences (9): 238-238. |
| [16] | Rajendran, V. and Lakshmi, K. S. (2008). In vitro and in vivo anti-inflammatory activity of leaves of Symplocos cochinchinesis (Lour) Moore ssp Laurina. Bangladesh Journal of Pharmacology. 3, 121-124. |
| [17] | Sabri, F. Z., Belarbi, M., Sabri, S. and Alsaydi M. (2012). Phytochemical screening and identification of some compounds from Mallow; J. Nat. Prod. Plant Reour., 2 (4): 512-516. |
| [18] | Sadique, J., Al-Rqobahs, W. A., Bughaith, and ElGindi, Ar. (1989). The bioactivity of certain medicinal plants on the stabilization of RBS membrane system. Fitoterapia. 60: 525-532. |
| [19] | Shenoy, S., Shwetha, K., Prabhu, K., Maradi, R., Bairy, K. L. and Shanbhang, T. (2010). Evaluation of anti-inflammatory activity of Tephrosia purpurea in rats. Asian Pac J Trop Med. 3 (3): 193-5. |
| [20] | Shoeb, M. (2006). Anticancer agents from medicinal plants. Bangladesh Journal of Pharmacology, 1, 35-41. |
| [21] | Tiwari, P., Bimlesh, K., Mandeep K., Gurpreet, K. and Harleen, K. (2011). Phytochemical screening and extraction: A review. Internationale Phrmaceuticasciencia, 1 (1): 1-9. |
| [22] | Van Wyk B. E and Gericke, N. (2000) People´s plants: A guide to useful plants of Southern Africa. Briza publications, Pretoria 226 (3/4): 245-247. |
| [23] | Von koenem, E. (2001). Medicinal, poisonous and edible plants in Namibia. Windhoek, Namibia; Gottingen: Klaus Hess publisher, 190-195. |
| [24] | Wugang. H (2008). Traditional Chinese medicinal plants and their endophytic fungi: isolation, identification and bio assay PhD thesis. University of Hong Kong. China, 1-17. |
APA Style
Barkoma Mohammed Bashir. (2023). Phytochemical Screening, Antioxidant, Anti-Inflammatory and Antibacterial Studies on the Stem Bark Extracts of Lannea microcarpa Engl. & K. Krause. Journal of Diseases and Medicinal Plants, 9(1), 21-32. https://doi.org/10.11648/j.jdmp.20230901.13
ACS Style
Barkoma Mohammed Bashir. Phytochemical Screening, Antioxidant, Anti-Inflammatory and Antibacterial Studies on the Stem Bark Extracts of Lannea microcarpa Engl. & K. Krause. J. Dis. Med. Plants 2023, 9(1), 21-32. doi: 10.11648/j.jdmp.20230901.13
AMA Style
Barkoma Mohammed Bashir. Phytochemical Screening, Antioxidant, Anti-Inflammatory and Antibacterial Studies on the Stem Bark Extracts of Lannea microcarpa Engl. & K. Krause. J Dis Med Plants. 2023;9(1):21-32. doi: 10.11648/j.jdmp.20230901.13
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Download@article{10.11648/j.jdmp.20230901.13,
author = {Barkoma Mohammed Bashir},
title = {Phytochemical Screening, Antioxidant, Anti-Inflammatory and Antibacterial Studies on the Stem Bark Extracts of Lannea microcarpa Engl. & K. Krause},
journal = {Journal of Diseases and Medicinal Plants},
volume = {9},
number = {1},
pages = {21-32},
doi = {10.11648/j.jdmp.20230901.13},
url = {https://doi.org/10.11648/j.jdmp.20230901.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20230901.13},
abstract = {Medicinal Plants are Plants used for traditional medicines. Any part of the plant could be used as medicine. Medicinal plants have been discovered and used in traditional medicine practices since pre-historical times. A survey of traditional medicine man in Damaturu indicated that Lannia microcarpa is a plants used in the treatment of inflammation, wounds and cancer. The fresh samples (stem bark) of the plant was collected and successively extracted with n-hexane, ethyl acetate, methanol and water. Phytochemical constituents of Lannea microcarpa extracts revealed the presence of cardiac glycosides, flavonoids, saponins, phenols, tannins, alkaloids, sterols and terpenoids. The antioxidant potential was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay, whereas membrane stabilization method was used to evaluate the anti-inflammatory potential of the extracts. The antibacterial potential of the plant extracts against four (4) wound pathogens: Salmonella typhi, Staphylococcus aureus, Escherichia coli and Strephtococcus pneumoneae, was evaluated using disc diffusion technician. The IC50 values obtained for the Lannea microcarpa extracts indicated that the methanol and water extracts exhibited higher antioxidant activities than the standard ascorbic acid. The extracts were found effective in inhibiting the heat induced haemolysis. Diclofenac Sodium salt used as standard drug at 100μg/ml offered 90.66% protection a significant (p<0.05) protection against damaging effect of heat solution. The results showed that all extracts from the plant offered a significant (p<0.05) protection against the damaging effect of hypotonic solution, Diclofenac sodium (100μg/ml), the standard drug offered a significant (p<0.05) protection (57.52%). Lannea microcarpa extracts showed zone of inhibition only against Salmonella typhi and Strephtococcus pneumonea.},
year = {2023}
}
TY - JOUR T1 - Phytochemical Screening, Antioxidant, Anti-Inflammatory and Antibacterial Studies on the Stem Bark Extracts of Lannea microcarpa Engl. & K. Krause AU - Barkoma Mohammed Bashir Y1 - 2023/03/31 PY - 2023 N1 - https://doi.org/10.11648/j.jdmp.20230901.13 DO - 10.11648/j.jdmp.20230901.13 T2 - Journal of Diseases and Medicinal Plants JF - Journal of Diseases and Medicinal Plants JO - Journal of Diseases and Medicinal Plants SP - 21 EP - 32 PB - Science Publishing Group SN - 2469-8210 UR - https://doi.org/10.11648/j.jdmp.20230901.13 AB - Medicinal Plants are Plants used for traditional medicines. Any part of the plant could be used as medicine. Medicinal plants have been discovered and used in traditional medicine practices since pre-historical times. A survey of traditional medicine man in Damaturu indicated that Lannia microcarpa is a plants used in the treatment of inflammation, wounds and cancer. The fresh samples (stem bark) of the plant was collected and successively extracted with n-hexane, ethyl acetate, methanol and water. Phytochemical constituents of Lannea microcarpa extracts revealed the presence of cardiac glycosides, flavonoids, saponins, phenols, tannins, alkaloids, sterols and terpenoids. The antioxidant potential was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay, whereas membrane stabilization method was used to evaluate the anti-inflammatory potential of the extracts. The antibacterial potential of the plant extracts against four (4) wound pathogens: Salmonella typhi, Staphylococcus aureus, Escherichia coli and Strephtococcus pneumoneae, was evaluated using disc diffusion technician. The IC50 values obtained for the Lannea microcarpa extracts indicated that the methanol and water extracts exhibited higher antioxidant activities than the standard ascorbic acid. The extracts were found effective in inhibiting the heat induced haemolysis. Diclofenac Sodium salt used as standard drug at 100μg/ml offered 90.66% protection a significant (p<0.05) protection against damaging effect of heat solution. The results showed that all extracts from the plant offered a significant (p<0.05) protection against the damaging effect of hypotonic solution, Diclofenac sodium (100μg/ml), the standard drug offered a significant (p<0.05) protection (57.52%). Lannea microcarpa extracts showed zone of inhibition only against Salmonella typhi and Strephtococcus pneumonea. VL - 9 IS - 1 ER -
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