Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats

Tatiana Kangah Mireille Kple; Joel Akakpo-Akue; Konan Armand Marcelin Kouassi; Sitapha Ouattara; Gbe Kouakou N’Dri Ange Konan; Diane Koulai; Larissa Adjoua Kouadio; Chiaye Claire Antoinette Yapo-Crezoit; Adou Koffi Mattieu Kra; Allico Joseph Djaman

doi:doi:10.11648/j.jdmp.20230902.12

Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats

Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Konan Armand Marcelin Kouassi, Sitapha Ouattara, Gbe Kouakou N’Dri Ange Konan, Diane Koulai, Larissa Adjoua Kouadio, Chiaye Claire Antoinette Yapo-Crezoit, Adou Koffi Mattieu Kra, Allico Joseph Djaman

Published in Journal of Diseases and Medicinal Plants (Volume 9, Issue 2)

Received: 8 March 2023 Accepted: 6 April 2023 Published: 27 April 2023

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Abstract

Anemia is characterized by an abnormal decrease in the level of hemoglobin or the number of red blood cells in the blood. It is a public health problem that mainly affects pregnant women and young children. In the present study, the objective was to evaluated the anti-anemic potential of a recipe composed of three medicinal plants, Xylopia aethiopica (Dunal) A. Rich, Zanthoxylum leprieurii (GUILL) and Harungara madagascariensis (LAM), used to treat sickle cell anemia in rats. Aqueous and hydroethanol extracts of recipe composed were first prepared and quantitative phytochemical tests were carried out. Then, the anti-anemic activity of the recipe extracts was assessed. The anemia was induced in animals by intraperitoneal injection of phenylhydrazine at a repeated dose of 40 mg/kg bw for two days. Treatment was carried out with the aqueous and hydroethanol extracts of the plant recipe at doses of 400 and 800 mg/kg bw. The obtained results indicated that recipe composed contains total phenols (43.31±0.92 mg GAE/g and 33.82±0.6 mg GAE /g of dry weight), total flavonoids (17.07±0.89 mg QE and 19.76±1.15 mg QE/g of dry weight) and total tannin (8.65±0.01 mg QE and 26.5±0.95 mg Cat E/g of dry weight) in decocted and hydroethanol extracts respectively. The anemia was effectively corrected after 21 days of oral administration of the extracts to the rats. The extracts significantly increased hemoglobin (P < 0.05), red blood cell count (P < 0.001), and hematocrit (P < 0.01) as early as the 7^th days of treatment compared with untreated controls. These results show that the plant recipe composed of Xylopia aethiopica, Zanthoxylum leprieurii and Harungara madagascariensis possess antianemic properties.

Published in	Journal of Diseases and Medicinal Plants (Volume 9, Issue 2)
DOI	10.11648/j.jdmp.20230902.12
Page(s)	40-48
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Anemia, Antianemic Potential, Phenylhydrazine, Medicinal Plants

References

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APA Style

Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Konan Armand Marcelin Kouassi, Sitapha Ouattara, Gbe Kouakou N’Dri Ange Konan, et al. (2023). Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats. Journal of Diseases and Medicinal Plants, 9(2), 40-48. https://doi.org/10.11648/j.jdmp.20230902.12

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ACS Style

Tatiana Kangah Mireille Kple; Joel Akakpo-Akue; Konan Armand Marcelin Kouassi; Sitapha Ouattara; Gbe Kouakou N’Dri Ange Konan, et al. Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats. J. Dis. Med. Plants 2023, 9(2), 40-48. doi: 10.11648/j.jdmp.20230902.12

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AMA Style

Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Konan Armand Marcelin Kouassi, Sitapha Ouattara, Gbe Kouakou N’Dri Ange Konan, et al. Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats. J Dis Med Plants. 2023;9(2):40-48. doi: 10.11648/j.jdmp.20230902.12

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@article{10.11648/j.jdmp.20230902.12,
  author = {Tatiana Kangah Mireille Kple and Joel Akakpo-Akue and Konan Armand Marcelin Kouassi and Sitapha Ouattara and Gbe Kouakou N’Dri Ange Konan and Diane Koulai and Larissa Adjoua Kouadio and Chiaye Claire Antoinette Yapo-Crezoit and Adou Koffi Mattieu Kra and Allico Joseph Djaman},
  title = {Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {9},
  number = {2},
  pages = {40-48},
  doi = {10.11648/j.jdmp.20230902.12},
  url = {https://doi.org/10.11648/j.jdmp.20230902.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20230902.12},
  abstract = {Anemia is characterized by an abnormal decrease in the level of hemoglobin or the number of red blood cells in the blood. It is a public health problem that mainly affects pregnant women and young children. In the present study, the objective was to evaluated the anti-anemic potential of a recipe composed of three medicinal plants, Xylopia aethiopica (Dunal) A. Rich, Zanthoxylum leprieurii (GUILL) and Harungara madagascariensis (LAM), used to treat sickle cell anemia in rats. Aqueous and hydroethanol extracts of recipe composed were first prepared and quantitative phytochemical tests were carried out. Then, the anti-anemic activity of the recipe extracts was assessed. The anemia was induced in animals by intraperitoneal injection of phenylhydrazine at a repeated dose of 40 mg/kg bw for two days. Treatment was carried out with the aqueous and hydroethanol extracts of the plant recipe at doses of 400 and 800 mg/kg bw. The obtained results indicated that recipe composed contains total phenols (43.31±0.92 mg GAE/g and 33.82±0.6 mg GAE /g of dry weight), total flavonoids (17.07±0.89 mg QE and 19.76±1.15 mg QE/g of dry weight) and total tannin (8.65±0.01 mg QE and 26.5±0.95 mg Cat E/g of dry weight) in decocted and hydroethanol extracts respectively. The anemia was effectively corrected after 21 days of oral administration of the extracts to the rats. The extracts significantly increased hemoglobin (P th days of treatment compared with untreated controls. These results show that the plant recipe composed of Xylopia aethiopica, Zanthoxylum leprieurii and Harungara madagascariensis possess antianemic properties.},
 year = {2023}
}

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TY  - JOUR
T1  - Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats
AU  - Tatiana Kangah Mireille Kple
AU  - Joel Akakpo-Akue
AU  - Konan Armand Marcelin Kouassi
AU  - Sitapha Ouattara
AU  - Gbe Kouakou N’Dri Ange Konan
AU  - Diane Koulai
AU  - Larissa Adjoua Kouadio
AU  - Chiaye Claire Antoinette Yapo-Crezoit
AU  - Adou Koffi Mattieu Kra
AU  - Allico Joseph Djaman
Y1  - 2023/04/27
PY  - 2023
N1  - https://doi.org/10.11648/j.jdmp.20230902.12
DO  - 10.11648/j.jdmp.20230902.12
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 40
EP  - 48
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20230902.12
AB  - Anemia is characterized by an abnormal decrease in the level of hemoglobin or the number of red blood cells in the blood. It is a public health problem that mainly affects pregnant women and young children. In the present study, the objective was to evaluated the anti-anemic potential of a recipe composed of three medicinal plants, Xylopia aethiopica (Dunal) A. Rich, Zanthoxylum leprieurii (GUILL) and Harungara madagascariensis (LAM), used to treat sickle cell anemia in rats. Aqueous and hydroethanol extracts of recipe composed were first prepared and quantitative phytochemical tests were carried out. Then, the anti-anemic activity of the recipe extracts was assessed. The anemia was induced in animals by intraperitoneal injection of phenylhydrazine at a repeated dose of 40 mg/kg bw for two days. Treatment was carried out with the aqueous and hydroethanol extracts of the plant recipe at doses of 400 and 800 mg/kg bw. The obtained results indicated that recipe composed contains total phenols (43.31±0.92 mg GAE/g and 33.82±0.6 mg GAE /g of dry weight), total flavonoids (17.07±0.89 mg QE and 19.76±1.15 mg QE/g of dry weight) and total tannin (8.65±0.01 mg QE and 26.5±0.95 mg Cat E/g of dry weight) in decocted and hydroethanol extracts respectively. The anemia was effectively corrected after 21 days of oral administration of the extracts to the rats. The extracts significantly increased hemoglobin (P th days of treatment compared with untreated controls. These results show that the plant recipe composed of Xylopia aethiopica, Zanthoxylum leprieurii and Harungara madagascariensis possess antianemic properties.
VL  - 9
IS  - 2
ER  -

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Author Information

Tatiana Kangah Mireille Kple

Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire
Joel Akakpo-Akue

Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire
Konan Armand Marcelin Kouassi

Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire
Sitapha Ouattara

Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire
Gbe Kouakou N’Dri Ange Konan

Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire
Diane Koulai

Food and Bioproducts Processes Laboratory, Department of Agronomic, Forestry and Environmental Engineering, University of Man, Man, C?te d’Ivoire
Larissa Adjoua Kouadio

Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire
Chiaye Claire Antoinette Yapo-Crezoit

Immunity Biology Pole, Pasteur Institute, Abidjan, C?te d’Ivoire
Adou Koffi Mattieu Kra

Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire
Allico Joseph Djaman

Laboratory of Biology and Health, Felix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire

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Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Konan Armand Marcelin Kouassi, Sitapha Ouattara, Gbe Kouakou N’Dri Ange Konan, et al. (2023). Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats. Journal of Diseases and Medicinal Plants, 9(2), 40-48. https://doi.org/10.11648/j.jdmp.20230902.12

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ACS Style

Tatiana Kangah Mireille Kple; Joel Akakpo-Akue; Konan Armand Marcelin Kouassi; Sitapha Ouattara; Gbe Kouakou N’Dri Ange Konan, et al. Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats. J. Dis. Med. Plants 2023, 9(2), 40-48. doi: 10.11648/j.jdmp.20230902.12

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AMA Style

Tatiana Kangah Mireille Kple, Joel Akakpo-Akue, Konan Armand Marcelin Kouassi, Sitapha Ouattara, Gbe Kouakou N’Dri Ange Konan, et al. Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats. J Dis Med Plants. 2023;9(2):40-48. doi: 10.11648/j.jdmp.20230902.12

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@article{10.11648/j.jdmp.20230902.12,
  author = {Tatiana Kangah Mireille Kple and Joel Akakpo-Akue and Konan Armand Marcelin Kouassi and Sitapha Ouattara and Gbe Kouakou N’Dri Ange Konan and Diane Koulai and Larissa Adjoua Kouadio and Chiaye Claire Antoinette Yapo-Crezoit and Adou Koffi Mattieu Kra and Allico Joseph Djaman},
  title = {Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {9},
  number = {2},
  pages = {40-48},
  doi = {10.11648/j.jdmp.20230902.12},
  url = {https://doi.org/10.11648/j.jdmp.20230902.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20230902.12},
  abstract = {Anemia is characterized by an abnormal decrease in the level of hemoglobin or the number of red blood cells in the blood. It is a public health problem that mainly affects pregnant women and young children. In the present study, the objective was to evaluated the anti-anemic potential of a recipe composed of three medicinal plants, Xylopia aethiopica (Dunal) A. Rich, Zanthoxylum leprieurii (GUILL) and Harungara madagascariensis (LAM), used to treat sickle cell anemia in rats. Aqueous and hydroethanol extracts of recipe composed were first prepared and quantitative phytochemical tests were carried out. Then, the anti-anemic activity of the recipe extracts was assessed. The anemia was induced in animals by intraperitoneal injection of phenylhydrazine at a repeated dose of 40 mg/kg bw for two days. Treatment was carried out with the aqueous and hydroethanol extracts of the plant recipe at doses of 400 and 800 mg/kg bw. The obtained results indicated that recipe composed contains total phenols (43.31±0.92 mg GAE/g and 33.82±0.6 mg GAE /g of dry weight), total flavonoids (17.07±0.89 mg QE and 19.76±1.15 mg QE/g of dry weight) and total tannin (8.65±0.01 mg QE and 26.5±0.95 mg Cat E/g of dry weight) in decocted and hydroethanol extracts respectively. The anemia was effectively corrected after 21 days of oral administration of the extracts to the rats. The extracts significantly increased hemoglobin (P th days of treatment compared with untreated controls. These results show that the plant recipe composed of Xylopia aethiopica, Zanthoxylum leprieurii and Harungara madagascariensis possess antianemic properties.},
 year = {2023}
}

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TY  - JOUR
T1  - Anti-Anemic Potential of a Herbal Recipe Against Phenylhydrazine-Induced Anemia in Rats
AU  - Tatiana Kangah Mireille Kple
AU  - Joel Akakpo-Akue
AU  - Konan Armand Marcelin Kouassi
AU  - Sitapha Ouattara
AU  - Gbe Kouakou N’Dri Ange Konan
AU  - Diane Koulai
AU  - Larissa Adjoua Kouadio
AU  - Chiaye Claire Antoinette Yapo-Crezoit
AU  - Adou Koffi Mattieu Kra
AU  - Allico Joseph Djaman
Y1  - 2023/04/27
PY  - 2023
N1  - https://doi.org/10.11648/j.jdmp.20230902.12
DO  - 10.11648/j.jdmp.20230902.12
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 40
EP  - 48
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20230902.12
AB  - Anemia is characterized by an abnormal decrease in the level of hemoglobin or the number of red blood cells in the blood. It is a public health problem that mainly affects pregnant women and young children. In the present study, the objective was to evaluated the anti-anemic potential of a recipe composed of three medicinal plants, Xylopia aethiopica (Dunal) A. Rich, Zanthoxylum leprieurii (GUILL) and Harungara madagascariensis (LAM), used to treat sickle cell anemia in rats. Aqueous and hydroethanol extracts of recipe composed were first prepared and quantitative phytochemical tests were carried out. Then, the anti-anemic activity of the recipe extracts was assessed. The anemia was induced in animals by intraperitoneal injection of phenylhydrazine at a repeated dose of 40 mg/kg bw for two days. Treatment was carried out with the aqueous and hydroethanol extracts of the plant recipe at doses of 400 and 800 mg/kg bw. The obtained results indicated that recipe composed contains total phenols (43.31±0.92 mg GAE/g and 33.82±0.6 mg GAE /g of dry weight), total flavonoids (17.07±0.89 mg QE and 19.76±1.15 mg QE/g of dry weight) and total tannin (8.65±0.01 mg QE and 26.5±0.95 mg Cat E/g of dry weight) in decocted and hydroethanol extracts respectively. The anemia was effectively corrected after 21 days of oral administration of the extracts to the rats. The extracts significantly increased hemoglobin (P th days of treatment compared with untreated controls. These results show that the plant recipe composed of Xylopia aethiopica, Zanthoxylum leprieurii and Harungara madagascariensis possess antianemic properties.
VL  - 9
IS  - 2
ER  -

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