Joint Detection of High-Risk HPV E6/E7 mRNA and HC2 HPV-DNA in Cervical Lesions Screening

Huang Shanshan; Xu Fengjuan; Cheng Yan; Gu Fenghua; Gu Guojian

doi:doi:10.11648/j.wjph.20230802.24

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Joint Detection of High-Risk HPV E6/E7 mRNA and HC2 HPV-DNA in Cervical Lesions Screening

Huang Shanshan, Xu Fengjuan, Cheng Yan, Gu Fenghua, Gu Guojian

Published in World Journal of Public Health (Volume 8, Issue 2)

Received: 3 April 2023 Accepted: 8 May 2023 Published: 22 May 2023

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Abstract

Objective To explore the value of joint detection of high-risk HPV E6/E7 mRNA and HC2 HPV-DNA to the screening of cervical lesions. Methods Totally 869 patients were divided into low-grade squamous intraepithelial lesions negative group including normal, inflammation and low-grade squamous intraepithelial lesions, and high-grade squamous intraepithelial lesions (HSIL) positive group including HSIL, squamous cell carcinoma and adenocarcinoma confirmed by ThinPrep cytologic test, HC2 HPV-DNA, high-risk HPV E6/E7 mRNA detection and colposcopy biopsy. The pathological result on HSIL positive was as the gold standard to evaluate the value of joint detection of HC2 HPV-DNA and high-risk HPV E6/E7 mRNA to the screening of cervical cancer. Results The positive rates of HPV E6/E7 mRNA and HPV-DNA detection in 869 patients were 46.1% and 61.3%, and increased with the severity of cytological grade and cervical level (P<0.05). The sensitivity and specificity of HPV E6/E7 mRNA were 97.4% and 64.9%, higher than those of HPV-DNA (89.0%, 44.6%) (P<0.05). The sensitivity and specificity of joint detection of HPV E6/E7 mRNA and HPV-DNA were 99.3% and 56.7%. The overall consistency rate and positive consistency rate were higher for HSIL positive (87.6%, 87.0%) than those for HSIL negative (70.5%, 30.5%) (P<0.05). Conclusion The positive expressions of HPV E6/E7 mRNA and HPV-DNA are closely correlated with the severity of cervical lesions, and the joint detection of them two has high sensitivity and specificity.

Published in	World Journal of Public Health (Volume 8, Issue 2)
DOI	10.11648/j.wjph.20230802.24
Page(s)	134-138
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Cervical Lesions, Human Papillomavirus, HPV E6/E7 mRNA, HPV-DNA

References

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APA Style

Huang Shanshan, Xu Fengjuan, Cheng Yan, Gu Fenghua, Gu Guojian. (2023). Joint Detection of High-Risk HPV E6/E7 mRNA and HC2 HPV-DNA in Cervical Lesions Screening. World Journal of Public Health, 8(2), 134-138. https://doi.org/10.11648/j.wjph.20230802.24

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ACS Style

Huang Shanshan; Xu Fengjuan; Cheng Yan; Gu Fenghua; Gu Guojian. Joint Detection of High-Risk HPV E6/E7 mRNA and HC2 HPV-DNA in Cervical Lesions Screening. World J. Public Health 2023, 8(2), 134-138. doi: 10.11648/j.wjph.20230802.24

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AMA Style

Huang Shanshan, Xu Fengjuan, Cheng Yan, Gu Fenghua, Gu Guojian. Joint Detection of High-Risk HPV E6/E7 mRNA and HC2 HPV-DNA in Cervical Lesions Screening. World J Public Health. 2023;8(2):134-138. doi: 10.11648/j.wjph.20230802.24

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@article{10.11648/j.wjph.20230802.24,
  author = {Huang Shanshan and Xu Fengjuan and Cheng Yan and Gu Fenghua and Gu Guojian},
  title = {Joint Detection of High-Risk HPV E6/E7 mRNA and HC2 HPV-DNA in Cervical Lesions Screening},
  journal = {World Journal of Public Health},
  volume = {8},
  number = {2},
  pages = {134-138},
  doi = {10.11648/j.wjph.20230802.24},
  url = {https://doi.org/10.11648/j.wjph.20230802.24},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjph.20230802.24},
  abstract = {Objective To explore the value of joint detection of high-risk HPV E6/E7 mRNA and HC2 HPV-DNA to the screening of cervical lesions. Methods Totally 869 patients were divided into low-grade squamous intraepithelial lesions negative group including normal, inflammation and low-grade squamous intraepithelial lesions, and high-grade squamous intraepithelial lesions (HSIL) positive group including HSIL, squamous cell carcinoma and adenocarcinoma confirmed by ThinPrep cytologic test, HC2 HPV-DNA, high-risk HPV E6/E7 mRNA detection and colposcopy biopsy. The pathological result on HSIL positive was as the gold standard to evaluate the value of joint detection of HC2 HPV-DNA and high-risk HPV E6/E7 mRNA to the screening of cervical cancer. Results The positive rates of HPV E6/E7 mRNA and HPV-DNA detection in 869 patients were 46.1% and 61.3%, and increased with the severity of cytological grade and cervical level (PPPConclusion The positive expressions of HPV E6/E7 mRNA and HPV-DNA are closely correlated with the severity of cervical lesions, and the joint detection of them two has high sensitivity and specificity.},
 year = {2023}
}

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TY  - JOUR
T1  - Joint Detection of High-Risk HPV E6/E7 mRNA and HC2 HPV-DNA in Cervical Lesions Screening
AU  - Huang Shanshan
AU  - Xu Fengjuan
AU  - Cheng Yan
AU  - Gu Fenghua
AU  - Gu Guojian
Y1  - 2023/05/22
PY  - 2023
N1  - https://doi.org/10.11648/j.wjph.20230802.24
DO  - 10.11648/j.wjph.20230802.24
T2  - World Journal of Public Health
JF  - World Journal of Public Health
JO  - World Journal of Public Health
SP  - 134
EP  - 138
PB  - Science Publishing Group
SN  - 2637-6059
UR  - https://doi.org/10.11648/j.wjph.20230802.24
AB  - Objective To explore the value of joint detection of high-risk HPV E6/E7 mRNA and HC2 HPV-DNA to the screening of cervical lesions. Methods Totally 869 patients were divided into low-grade squamous intraepithelial lesions negative group including normal, inflammation and low-grade squamous intraepithelial lesions, and high-grade squamous intraepithelial lesions (HSIL) positive group including HSIL, squamous cell carcinoma and adenocarcinoma confirmed by ThinPrep cytologic test, HC2 HPV-DNA, high-risk HPV E6/E7 mRNA detection and colposcopy biopsy. The pathological result on HSIL positive was as the gold standard to evaluate the value of joint detection of HC2 HPV-DNA and high-risk HPV E6/E7 mRNA to the screening of cervical cancer. Results The positive rates of HPV E6/E7 mRNA and HPV-DNA detection in 869 patients were 46.1% and 61.3%, and increased with the severity of cytological grade and cervical level (PPPConclusion The positive expressions of HPV E6/E7 mRNA and HPV-DNA are closely correlated with the severity of cervical lesions, and the joint detection of them two has high sensitivity and specificity.
VL  - 8
IS  - 2
ER  -

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Author Information

Huang Shanshan

Department of Obstetrics and Gynecology, Taicang Hospital Affiliated to Soochow University, Suzhou, China
Xu Fengjuan

Department of Obstetrics and Gynecology, Taicang Hospital Affiliated to Soochow University, Suzhou, China
Cheng Yan

Department of Obstetrics and Gynecology, Taicang Hospital Affiliated to Soochow University, Suzhou, China
Gu Fenghua

Department of Pathology, Taicang Hospital Affiliated to Soochow University, Suzhou, China
Gu Guojian

Department of Pathology, Taicang Hospital Affiliated to Soochow University, Suzhou, China

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Plain Text BibTeX RIS

APA Style

Huang Shanshan, Xu Fengjuan, Cheng Yan, Gu Fenghua, Gu Guojian. (2023). Joint Detection of High-Risk HPV E6/E7 mRNA and HC2 HPV-DNA in Cervical Lesions Screening. World Journal of Public Health, 8(2), 134-138. https://doi.org/10.11648/j.wjph.20230802.24

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ACS Style

Huang Shanshan; Xu Fengjuan; Cheng Yan; Gu Fenghua; Gu Guojian. Joint Detection of High-Risk HPV E6/E7 mRNA and HC2 HPV-DNA in Cervical Lesions Screening. World J. Public Health 2023, 8(2), 134-138. doi: 10.11648/j.wjph.20230802.24

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AMA Style

Huang Shanshan, Xu Fengjuan, Cheng Yan, Gu Fenghua, Gu Guojian. Joint Detection of High-Risk HPV E6/E7 mRNA and HC2 HPV-DNA in Cervical Lesions Screening. World J Public Health. 2023;8(2):134-138. doi: 10.11648/j.wjph.20230802.24

Copy | Download

@article{10.11648/j.wjph.20230802.24,
  author = {Huang Shanshan and Xu Fengjuan and Cheng Yan and Gu Fenghua and Gu Guojian},
  title = {Joint Detection of High-Risk HPV E6/E7 mRNA and HC2 HPV-DNA in Cervical Lesions Screening},
  journal = {World Journal of Public Health},
  volume = {8},
  number = {2},
  pages = {134-138},
  doi = {10.11648/j.wjph.20230802.24},
  url = {https://doi.org/10.11648/j.wjph.20230802.24},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjph.20230802.24},
  abstract = {Objective To explore the value of joint detection of high-risk HPV E6/E7 mRNA and HC2 HPV-DNA to the screening of cervical lesions. Methods Totally 869 patients were divided into low-grade squamous intraepithelial lesions negative group including normal, inflammation and low-grade squamous intraepithelial lesions, and high-grade squamous intraepithelial lesions (HSIL) positive group including HSIL, squamous cell carcinoma and adenocarcinoma confirmed by ThinPrep cytologic test, HC2 HPV-DNA, high-risk HPV E6/E7 mRNA detection and colposcopy biopsy. The pathological result on HSIL positive was as the gold standard to evaluate the value of joint detection of HC2 HPV-DNA and high-risk HPV E6/E7 mRNA to the screening of cervical cancer. Results The positive rates of HPV E6/E7 mRNA and HPV-DNA detection in 869 patients were 46.1% and 61.3%, and increased with the severity of cytological grade and cervical level (PPPConclusion The positive expressions of HPV E6/E7 mRNA and HPV-DNA are closely correlated with the severity of cervical lesions, and the joint detection of them two has high sensitivity and specificity.},
 year = {2023}
}

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TY  - JOUR
T1  - Joint Detection of High-Risk HPV E6/E7 mRNA and HC2 HPV-DNA in Cervical Lesions Screening
AU  - Huang Shanshan
AU  - Xu Fengjuan
AU  - Cheng Yan
AU  - Gu Fenghua
AU  - Gu Guojian
Y1  - 2023/05/22
PY  - 2023
N1  - https://doi.org/10.11648/j.wjph.20230802.24
DO  - 10.11648/j.wjph.20230802.24
T2  - World Journal of Public Health
JF  - World Journal of Public Health
JO  - World Journal of Public Health
SP  - 134
EP  - 138
PB  - Science Publishing Group
SN  - 2637-6059
UR  - https://doi.org/10.11648/j.wjph.20230802.24
AB  - Objective To explore the value of joint detection of high-risk HPV E6/E7 mRNA and HC2 HPV-DNA to the screening of cervical lesions. Methods Totally 869 patients were divided into low-grade squamous intraepithelial lesions negative group including normal, inflammation and low-grade squamous intraepithelial lesions, and high-grade squamous intraepithelial lesions (HSIL) positive group including HSIL, squamous cell carcinoma and adenocarcinoma confirmed by ThinPrep cytologic test, HC2 HPV-DNA, high-risk HPV E6/E7 mRNA detection and colposcopy biopsy. The pathological result on HSIL positive was as the gold standard to evaluate the value of joint detection of HC2 HPV-DNA and high-risk HPV E6/E7 mRNA to the screening of cervical cancer. Results The positive rates of HPV E6/E7 mRNA and HPV-DNA detection in 869 patients were 46.1% and 61.3%, and increased with the severity of cytological grade and cervical level (PPPConclusion The positive expressions of HPV E6/E7 mRNA and HPV-DNA are closely correlated with the severity of cervical lesions, and the joint detection of them two has high sensitivity and specificity.
VL  - 8
IS  - 2
ER  -

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