Determination of Cotinine and Trans-3’-Hydroxycotinine in Human Serum by LC-MS/MS and Its Application

Daguang Wang; Kehe Du; Lijun Shao; Shiqiao Ma; Xiaofeng Yang; Tianyi Liu; Qian Liu; Meng Wang; Hongjun Liu; Yiqun Wu

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Determination of Cotinine and Trans-3’-Hydroxycotinine in Human Serum by LC-MS/MS and Its Application

Daguang Wang, Kehe Du, Lijun Shao, Shiqiao Ma, Xiaofeng Yang, Tianyi Liu, Qian Liu, Meng Wang, Hongjun Liu, Yiqun Wu

Published in Asia-Pacific Journal of Health Science (Volume 1, Issue 1)

Received: 20 March 2019 Accepted: 25 April 2019 Published: 30 April 2019

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Abstract

OBJECTIVE: An LC-MS/MS method for determination of Cotinine and Trans-3’-Hydroxycotinine in human serum was developed. Establish a methodology basis for studying tobacco exposure and health.METHOD: After protein precipitation with acetonitrile, the analytes and internal standard (I.S.), Cotinine-d3, were separated on a ZORBAX SB-Phenyl analytical column using the mobile phase of methanol (containing 0.3% formic acid) and water (containing 0.15% formic acid). Detection was carried out by electrospray positive ionization mass spectrometry in the multiple reaction monitoring (MRM) mode. RESULT: Cotinine , Trans-3’-Hydroxycotinine and I.S. were eluted at 2.56 min, 1.58min and 2.56 min, respectively. The calibration curves were linear and ranges for cotinine, trans-3’-Hydroxycotinine were respectively 0.05-500 ng·mL^-1 and 0.50~1250 ng·mL^-1. The lower limit of quantitation (LLOQ) was 0.05 ng·mL^-1 for cotinine and 0.50 ng·mL^-1 for trans-3’-Hydroxycotinine.Inter- and intra-day relative standard deviations were both less than 11%, and the relative errors were within ±7%. The mean extract recoveries were 98.54% (Cotinine)and 100.24% (trans-3’-Hydroxycotinine). CONCLUSION: It is a rapid, sensitive, selective and reliable method for the determination of cotinine and trans-3’-Hydroxycotinine in human serum in human serum. The cutoff value can be determined by measuring the content of cotinine in human serum, which provides a basis for distinguishing between smoking and non-smoking.

Published in	Asia-Pacific Journal of Health Science (Volume 1, Issue 1)
Page(s)	5-13
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Cotinine, Trans-3’-hydroxycotinine, Human Serum, LC-MS/MS, CUTOFF Value

References

[1]	World Health organization. Fact sheet 339-Tobacco.[2018-03-09].http//who.int/zh/news room/fact sheets/detail/tabacco.
[2]	王俊, 宋瑞金. 人体内可替宁检测技术研究进展[J]. 环境与健康杂志, 2008, 25(12): 1111-1114.
[3]	葛新, 郑频频, 傅华. 被动吸烟尼古丁及其代谢产物的暴露评价[J]. 中国卫生检验杂志, 2011, 21(7): 1840-1842.
[4]	Jaakkola MS，Ma J，Yang G，et a1．Determinants of salivary cotinine concentrations in Chinese male smokers[J].Prev Med,2003,36:282-290．
[5]	Page-Sharp M，Hale Tw，Hackett LP，et a1．Measurement of nicotine and eotinine in hunaan milk by high-performance liquid chromatography with ultraviolet absorbance detection [J]. J Chromatogr B Anal Technol Biomed Lifc Sci.2003,796:173—180．
[6]	Peyton Jacob III, Lisa Yu, M injiang Duan, et al. Determination of the Nicotine Metabolites Cotinine and Trans-3’-Hydroxycotinine in Biologic fluids of Smokers and Non-Smokers using Liquid Chromatography-Tandem Mass Spectrometry:Biomarkers for Tobacco Smoke Exposure and for Phenotyping Cytochrome P450 2A6 Activity.[J]. Journal of Chromatography B,2011, 879(3-4):267-276.
[7]	Chao Yuan,Justin Kosewick,Sihe Wang. A simple, fast, and sensitive method for the measurement of serum nicotine, cotinine,and nornicotine by LC–MS/MS [J]. J. Sep. Sci. 2013,36:2394–2400.
[8]	Maria Dobrinasa, Eva Choonga, Muriel Noetzlia, et al. Quantification of nicotine, cotinine, trans-3_-hydroxycotinine and varenicline in human plasma by a sensitive and specific UPLC–tandem mass-spectrometry procedure for a clinical study on smoking cessation [J]. Journal of Chromatography B, 2011,879: 3574-3582 .
[9]	杨睿悦，周伟燕，张天娇,等. 同位素稀释液相色谱串联质谱法测定血清可替宁[J],中华检验医学杂志,2012,35(4):333-338.
[10]	Stead LF, Bergson G, Lancaster T. Physician advice for smoking cessation. Cochrane Database Syst Rev 2008:CD000165.
[11]	Ko K , Kwon M J , Yang S H , et al. Evaluation of Serum Cotinine Cut-Off to Distinguish Smokers From Nonsmokers in the Korean Population[J]. Annals of Laboratory Medicine, 2016, 36(5).

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APA Style

Daguang Wang, Kehe Du, Lijun Shao, Shiqiao Ma, Xiaofeng Yang, et al. (2019). Determination of Cotinine and Trans-3’-Hydroxycotinine in Human Serum by LC-MS/MS and Its Application. Asia-Pacific Journal of Health Science, 1(1), 5-13.

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ACS Style

Daguang Wang; Kehe Du; Lijun Shao; Shiqiao Ma; Xiaofeng Yang, et al. Determination of Cotinine and Trans-3’-Hydroxycotinine in Human Serum by LC-MS/MS and Its Application. Asia-Pac. J. Health Sci. 2019, 1(1), 5-13.

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AMA Style

Daguang Wang, Kehe Du, Lijun Shao, Shiqiao Ma, Xiaofeng Yang, et al. Determination of Cotinine and Trans-3’-Hydroxycotinine in Human Serum by LC-MS/MS and Its Application. Asia-Pac J Health Sci. 2019;1(1):5-13.

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@article{10038384,
  author = {Daguang Wang and Kehe Du and Lijun Shao and Shiqiao Ma and Xiaofeng Yang and Tianyi Liu and Qian Liu and Meng Wang and Hongjun Liu and Yiqun Wu},
  title = {Determination of Cotinine and Trans-3’-Hydroxycotinine in Human Serum by LC-MS/MS and Its Application},
  journal = {Asia-Pacific Journal of Health Science},
  volume = {1},
  number = {1},
  pages = {5-13},
  url = {https://www.sciencepublishinggroup.com/article/10038384},
  abstract = {OBJECTIVE: An LC-MS/MS method for determination of Cotinine and Trans-3’-Hydroxycotinine in human serum was developed. Establish a methodology basis for studying tobacco exposure and health.METHOD: After protein precipitation with acetonitrile, the analytes and internal standard (I.S.), Cotinine-d3, were separated on a ZORBAX SB-Phenyl analytical column using the mobile phase of methanol (containing 0.3% formic acid) and water (containing 0.15% formic acid). Detection was carried out by electrospray positive ionization mass spectrometry in the multiple reaction monitoring (MRM) mode. RESULT: Cotinine , Trans-3’-Hydroxycotinine and I.S. were eluted at 2.56 min, 1.58min and 2.56 min, respectively. The calibration curves were linear and ranges for cotinine, trans-3’-Hydroxycotinine were respectively 0.05-500 ng·mL-1 and 0.50~1250 ng·mL-1. The lower limit of quantitation (LLOQ) was 0.05 ng·mL-1 for cotinine and 0.50 ng·mL-1 for trans-3’-Hydroxycotinine.Inter- and intra-day relative standard deviations were both less than 11%, and the relative errors were within ±7%. The mean extract recoveries were 98.54% (Cotinine)and 100.24% (trans-3’-Hydroxycotinine). CONCLUSION: It is a rapid, sensitive, selective and reliable method for the determination of cotinine and trans-3’-Hydroxycotinine in human serum in human serum. The cutoff value can be determined by measuring the content of cotinine in human serum, which provides a basis for distinguishing between smoking and non-smoking.},
 year = {2019}
}

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TY  - JOUR
T1  - Determination of Cotinine and Trans-3’-Hydroxycotinine in Human Serum by LC-MS/MS and Its Application
AU  - Daguang Wang
AU  - Kehe Du
AU  - Lijun Shao
AU  - Shiqiao Ma
AU  - Xiaofeng Yang
AU  - Tianyi Liu
AU  - Qian Liu
AU  - Meng Wang
AU  - Hongjun Liu
AU  - Yiqun Wu
Y1  - 2019/04/30
PY  - 2019
T2  - Asia-Pacific Journal of Health Science
JF  - Asia-Pacific Journal of Health Science
JO  - Asia-Pacific Journal of Health Science
SP  - 5
EP  - 13
PB  - Science Publishing Group
UR  - http://www.sciencepg.com/article/10038384
AB  - OBJECTIVE: An LC-MS/MS method for determination of Cotinine and Trans-3’-Hydroxycotinine in human serum was developed. Establish a methodology basis for studying tobacco exposure and health.METHOD: After protein precipitation with acetonitrile, the analytes and internal standard (I.S.), Cotinine-d3, were separated on a ZORBAX SB-Phenyl analytical column using the mobile phase of methanol (containing 0.3% formic acid) and water (containing 0.15% formic acid). Detection was carried out by electrospray positive ionization mass spectrometry in the multiple reaction monitoring (MRM) mode. RESULT: Cotinine , Trans-3’-Hydroxycotinine and I.S. were eluted at 2.56 min, 1.58min and 2.56 min, respectively. The calibration curves were linear and ranges for cotinine, trans-3’-Hydroxycotinine were respectively 0.05-500 ng·mL-1 and 0.50~1250 ng·mL-1. The lower limit of quantitation (LLOQ) was 0.05 ng·mL-1 for cotinine and 0.50 ng·mL-1 for trans-3’-Hydroxycotinine.Inter- and intra-day relative standard deviations were both less than 11%, and the relative errors were within ±7%. The mean extract recoveries were 98.54% (Cotinine)and 100.24% (trans-3’-Hydroxycotinine). CONCLUSION: It is a rapid, sensitive, selective and reliable method for the determination of cotinine and trans-3’-Hydroxycotinine in human serum in human serum. The cutoff value can be determined by measuring the content of cotinine in human serum, which provides a basis for distinguishing between smoking and non-smoking.
VL  - 1
IS  - 1
ER  -

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Author Information

Daguang Wang

Beijing Hospital Laboratory, National Center for Gerontology, Beijing, China
Kehe Du

Iphase Pharma Laboratory, Iphase Pharma Services (Think Tank Research Center for Health Development Laboratory), Beijing, China
Lijun Shao

Iphase Pharma Laboratory, Iphase Pharma Services (Think Tank Research Center for Health Development Laboratory), Beijing, China
Shiqiao Ma

Iphase Pharma Laboratory, Iphase Pharma Services (Think Tank Research Center for Health Development Laboratory), Beijing, China
Xiaofeng Yang

Beijing Hospital Laboratory, National Center for Gerontology, Beijing, China
Tianyi Liu

Beijing Hospital Laboratory, National Center for Gerontology, Beijing, China
Qian Liu

Beijing Hospital Laboratory, National Center for Gerontology, Beijing, China
Meng Wang

Beijing Hospital Laboratory, National Center for Gerontology, Beijing, China
Hongjun Liu

Iphase Pharma Laboratory, Iphase Pharma Services (Think Tank Research Center for Health Development Laboratory), Beijing, China
Yiqun Wu

Iphase Pharma Laboratory, Iphase Pharma Services (Think Tank Research Center for Health Development Laboratory), Beijing, China

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Plain Text BibTeX RIS

APA Style

Daguang Wang, Kehe Du, Lijun Shao, Shiqiao Ma, Xiaofeng Yang, et al. (2019). Determination of Cotinine and Trans-3’-Hydroxycotinine in Human Serum by LC-MS/MS and Its Application. Asia-Pacific Journal of Health Science, 1(1), 5-13.

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ACS Style

Daguang Wang; Kehe Du; Lijun Shao; Shiqiao Ma; Xiaofeng Yang, et al. Determination of Cotinine and Trans-3’-Hydroxycotinine in Human Serum by LC-MS/MS and Its Application. Asia-Pac. J. Health Sci. 2019, 1(1), 5-13.

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AMA Style

Daguang Wang, Kehe Du, Lijun Shao, Shiqiao Ma, Xiaofeng Yang, et al. Determination of Cotinine and Trans-3’-Hydroxycotinine in Human Serum by LC-MS/MS and Its Application. Asia-Pac J Health Sci. 2019;1(1):5-13.

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@article{10038384,
  author = {Daguang Wang and Kehe Du and Lijun Shao and Shiqiao Ma and Xiaofeng Yang and Tianyi Liu and Qian Liu and Meng Wang and Hongjun Liu and Yiqun Wu},
  title = {Determination of Cotinine and Trans-3’-Hydroxycotinine in Human Serum by LC-MS/MS and Its Application},
  journal = {Asia-Pacific Journal of Health Science},
  volume = {1},
  number = {1},
  pages = {5-13},
  url = {https://www.sciencepublishinggroup.com/article/10038384},
  abstract = {OBJECTIVE: An LC-MS/MS method for determination of Cotinine and Trans-3’-Hydroxycotinine in human serum was developed. Establish a methodology basis for studying tobacco exposure and health.METHOD: After protein precipitation with acetonitrile, the analytes and internal standard (I.S.), Cotinine-d3, were separated on a ZORBAX SB-Phenyl analytical column using the mobile phase of methanol (containing 0.3% formic acid) and water (containing 0.15% formic acid). Detection was carried out by electrospray positive ionization mass spectrometry in the multiple reaction monitoring (MRM) mode. RESULT: Cotinine , Trans-3’-Hydroxycotinine and I.S. were eluted at 2.56 min, 1.58min and 2.56 min, respectively. The calibration curves were linear and ranges for cotinine, trans-3’-Hydroxycotinine were respectively 0.05-500 ng·mL-1 and 0.50~1250 ng·mL-1. The lower limit of quantitation (LLOQ) was 0.05 ng·mL-1 for cotinine and 0.50 ng·mL-1 for trans-3’-Hydroxycotinine.Inter- and intra-day relative standard deviations were both less than 11%, and the relative errors were within ±7%. The mean extract recoveries were 98.54% (Cotinine)and 100.24% (trans-3’-Hydroxycotinine). CONCLUSION: It is a rapid, sensitive, selective and reliable method for the determination of cotinine and trans-3’-Hydroxycotinine in human serum in human serum. The cutoff value can be determined by measuring the content of cotinine in human serum, which provides a basis for distinguishing between smoking and non-smoking.},
 year = {2019}
}

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TY  - JOUR
T1  - Determination of Cotinine and Trans-3’-Hydroxycotinine in Human Serum by LC-MS/MS and Its Application
AU  - Daguang Wang
AU  - Kehe Du
AU  - Lijun Shao
AU  - Shiqiao Ma
AU  - Xiaofeng Yang
AU  - Tianyi Liu
AU  - Qian Liu
AU  - Meng Wang
AU  - Hongjun Liu
AU  - Yiqun Wu
Y1  - 2019/04/30
PY  - 2019
T2  - Asia-Pacific Journal of Health Science
JF  - Asia-Pacific Journal of Health Science
JO  - Asia-Pacific Journal of Health Science
SP  - 5
EP  - 13
PB  - Science Publishing Group
UR  - http://www.sciencepg.com/article/10038384
AB  - OBJECTIVE: An LC-MS/MS method for determination of Cotinine and Trans-3’-Hydroxycotinine in human serum was developed. Establish a methodology basis for studying tobacco exposure and health.METHOD: After protein precipitation with acetonitrile, the analytes and internal standard (I.S.), Cotinine-d3, were separated on a ZORBAX SB-Phenyl analytical column using the mobile phase of methanol (containing 0.3% formic acid) and water (containing 0.15% formic acid). Detection was carried out by electrospray positive ionization mass spectrometry in the multiple reaction monitoring (MRM) mode. RESULT: Cotinine , Trans-3’-Hydroxycotinine and I.S. were eluted at 2.56 min, 1.58min and 2.56 min, respectively. The calibration curves were linear and ranges for cotinine, trans-3’-Hydroxycotinine were respectively 0.05-500 ng·mL-1 and 0.50~1250 ng·mL-1. The lower limit of quantitation (LLOQ) was 0.05 ng·mL-1 for cotinine and 0.50 ng·mL-1 for trans-3’-Hydroxycotinine.Inter- and intra-day relative standard deviations were both less than 11%, and the relative errors were within ±7%. The mean extract recoveries were 98.54% (Cotinine)and 100.24% (trans-3’-Hydroxycotinine). CONCLUSION: It is a rapid, sensitive, selective and reliable method for the determination of cotinine and trans-3’-Hydroxycotinine in human serum in human serum. The cutoff value can be determined by measuring the content of cotinine in human serum, which provides a basis for distinguishing between smoking and non-smoking.
VL  - 1
IS  - 1
ER  -

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