Plant Responses on Elective Gels for in vitro Shoot Multiplication and Root Elongation of Pineapple (Ananas comosus L.)
Issue:
Volume 6, Issue 1, June 2022
Pages:
1-4
Received:
25 March 2022
Accepted:
25 April 2022
Published:
10 May 2022
Abstract: The quest for alternative matrices for plant tissue culture is a continuing process. Agar is one of the utmost common coagulating agents in plant in vitro clonal propagation. Pure grade agar has high value price and fear of over manipulation of its resources affected searching for low cost options. Therefore, liquid medium supplement with silica gel, glass beads and sands substratum and agar in two steps of micro propagation (seedling proliferation and root induction) were investigated. The shoot multiplication in the cytokinins hormone of 2.5mg/l BA and 0.5mg/l KN supported by 7g/l agar and alternative matrices has been produced 33.1 shoots and 5.3 shoot length in sands and agar. There is none significant difference among shoot number and shoot length on the agar and sands on shoot multiplication and plant, and there is significant difference in glass beads and silica gel compared with sand/agar in the shoot multiplication, and there is highly significance in shoot length in the matrices of plant support. Pineapple root induced in auxins hormone in vitro culture of plant propagation in half strength MS media, 30g/l sugar, 1mg/l NAA and 7g/l agar. The higher root number in sands 10.6 and followed 10 in silica gel and agar supported matrices. The root length also none significant difference among means treatments in agar, glass beads and sands. However, there is a significance difference with silica gel in root length. These silica gel, glass beads and sands used as agar gels to support and aeration plantlets.
Abstract: The quest for alternative matrices for plant tissue culture is a continuing process. Agar is one of the utmost common coagulating agents in plant in vitro clonal propagation. Pure grade agar has high value price and fear of over manipulation of its resources affected searching for low cost options. Therefore, liquid medium supplement with silica ge...
Show More
Screening of Some Ethiopian Wheat Lines Against Stem Rust Races at Seedling Stage
Mekonnen Assefa,
Kitessa Gutu
Issue:
Volume 6, Issue 1, June 2022
Pages:
5-9
Received:
18 May 2022
Accepted:
22 June 2022
Published:
30 June 2022
Abstract: One of the most harmful wheat diseases in Ethiopia is stem rust caused by Puccinia graminis f. sp. tritici (Pgt). It has the capacity to result in yield losses of up to 100% on susceptible cultivars. In a study, forty-two wheat lines along with one susceptible check were tested for their ability to fight off eleven isolates of the stem rust races TTKSK (Ug99 race), TTRTF, TKTTF (Digalu race), JRCQC, TKKTF, TRTTF, TTTTF, TTKTF, TTKTT, RRTTF, and TKPTF. In the Ambo Plant Protection Research Center in 2021, wheat lines were experimentally infected with urediniospores of the various races under controlled circumstances. Different seedling resistance responses were seen against the Pgt races that were investigated. Thirteen wheat lines (WL-1, WL-4, WL-21, WL-35, WL-37, WL-38, WL-41, WL-43, WL-45, WL-48, WL-49, WL-61, WL-64), which demonstrated low infection types (ITs of 2 or lower) against all the races, resulted in twenty-three lines having both resistant and susceptible infection types (ITs 0 to 3), In contrast, susceptible checks and six wheat lines (WL-26, WL-31, WL-33, WL-47, WL-51, WL-66) consistently showed susceptible infection types (ITs 2+ to 3+) to all races. The study's found resistant wheat lines can be employed as sources of resistance in the breeding program for wheat.
Abstract: One of the most harmful wheat diseases in Ethiopia is stem rust caused by Puccinia graminis f. sp. tritici (Pgt). It has the capacity to result in yield losses of up to 100% on susceptible cultivars. In a study, forty-two wheat lines along with one susceptible check were tested for their ability to fight off eleven isolates of the stem rust races T...
Show More
