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Brillantasia patula Aqueous Leaf Extract Averts Hyperglycermia, Lipid Peroxidation, and Alterations in Hematological Parameters in Alloxan-Induced Diabetic Rats

The protective effects of aqueous leaf extract of Brillantasia patula against hyperglycermia, lipid peroxidation, and alterations in hematological parameters in diabetic Wistar rats were investigated. The study consisted of six treatment groups, with five animals each, designated as Group-1 (healthy), Group-2 (diabetic control) and Groups 3-6 (diabetic rats treated with 500, 1000, 1500, and 2000 mg/kgbwt of extract, respectively). Rats were administered their respective doses orally, and daily, for 14 days. Thereafter, the effects on serum glucose levels, liver and kidney functions, lipid peroxidation, free radical scavenger and hematological parameters were analyzed. Blood glucose levels reduced markedly in diabetic rats given the plant extract relative to diabetic control. Both serum creatinine and urea decreased significantly in treated diabetic rats at extract doses of 1000 mg/kgbwt and above. Reductions in serum cholesterol (p<0.05) and triglyceride levels (p<0.05) were also observed. Elevated total serum protein and globulin in diabetic control was decreased in all treated groups. Haematological indices of groups given the extract were noticeably enhanced. Similarly, kidney, heart and liver glutathione (GSH) levels increased significantly in groups treated compared to diabetic control; lipid peroxidation in kidney and heart also decreased significantly in all the treated groups. Liver catalase activity improved. Serum alanine and aspartate aminotransferases activities widely lowered in Groups 3 and 4. The study indicates that Brillantaisia patula aqueous leaf extract exhibits potential hypoglycemic effect, prevents lipid peroxidation, boosts haematological parameters, and could protect liver and renal damage associated with diabetes especially at doses of 500 - 1000 mg/kgbwt.

Diabetes Mellitus, Brillantasia patula, Hypoglycemic Effect, Lipid Peroxidation, Antioxidants, Haematological Indices

Akpovwehwee Akporhuarho Anigboro, Oghenetega Jonathan Avwioroko, Nyerhovwo John Tonukari. (2018). Brillantasia patula Aqueous Leaf Extract Averts Hyperglycermia, Lipid Peroxidation, and Alterations in Hematological Parameters in Alloxan-Induced Diabetic Rats. International Journal of Biomedical Science and Engineering, 6(2), 43-51.

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1. Adoum, O. A., Micheal, B. O., Mohammad, I. S. Phytochemicals and hypoglycaemic effect of methanol stem-bark extract of Ficus sycomorus Linn (Moraceae) on alloxan induced diabetic Wistar albino rats. Afri J Biotechnol 2012; 11(17): 4095-4097.
2. Onyesom, I., Ogbodu, E. D., Osioma, E., Avwioroko, O. J. Injectable progestin contraceptive could increase the risk of insulin resistance syndrome in humans. Inter J Biochem Photon 2013; 108: 225-230.
3. Sassy-Prigent, C., Heudes, D., Jouquey, S., Auberval, D., Beliar, M., Michel, O., Hamon, G., Schwartz, C., Valente, A., Sprague, E. (1995) A modern view of atherogenesis. Am. J. Cardiol. 71:9-4.
4. Arise, R., Malomo, S., Adebayo, J., Igunnu, A. Effects of aqueous extract of Eucalyptus globules on lipid peroxidation and selected enzymes of rat’s liver. J. Med. Plant Res 2009; 077-081.
5. Anigboro, A. A, Onakurhefe, P., Tonukari, N. J., Avwioroko, O. J., Egbeme E. Quantitative determination of some phytochemicals (phenol, flavonoid, saponin and alkaloid) in twenty-two Nigerian medicinal plants. Nig J Sci Environ 2014; 13(1):86-93.
6. Marles, J., Farnsworth, N. Antidiabetic plants and their active constituents. Phytomedicine 1995; 2(2):123-185.
7. Orororo, O. C., Tonukari, N. J., Avwioroko, O. J., Ezedom, T. Effect of supplementation of animal feed with dried cassava (Manihot esculenta) peels, and stems of Vernonia amygdalina and Pennisetum purpereum on some biochemical parameters in pigs. NISEB J 2014; 14(4): 177-183.
8. Anigboro, A. A, Avwioroko, O. J., Ohwokevwo, O. A., Nzor, J. N. Phytochemical constituents, antidiabetic and ameliorative effects of Polyalthia longifiola leaf extract against alterations in hepatic and renal functions in alloxan-induced diabetic rats. J. Appl. Sci. Environ. Manag. 2018; In Press (Accepted).
9. Gupta, R. C., Chang, D., Nammi, S., Bensoussan, A., Bilinski, K., Roufogalis, B. D. Interactions between antidiabetic drugs and herbs: an overview of mechanisms of action and clinical implications. Diabetol. Metabol. syndrome 2017; 9(1):59.
10. Chudasama, R. G., Dhanani, N. J., Amrutiya, R. M., Chandni, R., Jayanthi, G., Karthikeyan, K. Screening of selected plants from semi-arid region for its phytochemical constituents and antimicrobial activity. J. Pharmacog. Phytochem. 2018; 7(2): 2983-2988.
11. Yamagishi, S. I., Matsui, T., Ishibashi, Y., Isami, F., Abe, Y., Sakaguchi, T., Higashimoto, Y. Phytochemicals against advanced glycation end products (AGEs) and the receptor system. Curr. Pharmaceut. Design 2017; 23(8): 1135-1141.
12. Makambila-Koubemba, M., Mbatchi, B., Ardid, D., Gelot, A., Henrion, C. et al. Pharmacological studies of ten medicinal plants used for analgesic purposes in congo Brazzaville. Int. J. Pharmacol 2011; 7:608-615.
13. Allain, C. C., Poon, L. S. Cicely, S. G. Enzymatic Determination of Total Serum Cholesterol. Clin. Chem 1974; 20: 470-475
14. Fossati, P. C., L. Prencipe. Triglycerides determination after enzymatic hydorolysis, Clin. Chem 1982; 28: 2077.
15. Nakamura, W., Hojoda, S., Hayashi, K. Purification and Properties of Rat Liver Glutathione Peroxidase. Biochimica et Biophysica Acta 1974; 358: 251-261.
16. Deniz S., Arzu S., Figen I., Gulden C. Lipid peroxidation and antioxidant status in experimental animals. Effects of aging and hypercholesterolemic diet. Clin. Chem. Acta 1997; 265: 77-82.
17. Reitman S., Frankel, S. Colorimetric Method for Determination of Serum GOT and GPT. Am J Clin Pathol 1957; 28-56.
18. Bartels H., Boehmer M. Microdetermination of creatinine. Clin. Chim. Acta 1971: 32:81–85.
19. Lyne, E. Spectrophotometric and Turbidometric Methods for Measuring Proteins. Methods in Enzymol 1957; 10: 447-455.
20. Palton C. J., Crouch S. R. Spectrophotometric and kinetics investigation of the Berthelot reaction for the determination of ammonia. Anal Chem 1977; 49:464-469.
21. Cheesbrough, M. District Laboratory Practice in Tropical Countries, part 2. Cambridge University Press 2004; pp 249-258.
22. Aebi H. Catalase in vitro. In: Methods in Enzymology. Academic Press, New York, 1984; pp. 479-500.
23. Hjort, R., Alfredsson, L., Andersson, T., Carlsson, P. O., Grill, V., Groop, L., et al. Family history of type 1 and type 2 diabetes and risk of latent autoimmune diabetes in adults (LADA). Diabetes and metabolism 2017; 43(6): 536-542.
24. Swasey, K. K., Orchard, T. J., Costacou, T. Trends in cardiovascular risk factor management in type 1 diabetes by sex. J Diabetes Complic 2018; 32(4): 411-417.
25. Akindele OA, Babatunde AI, Chinedu FM, Samuel OA, Oluwasola CA, Oluseyi AA. Rat model of food induced non-obese-type 2 diabetes mellitus; comparative pathophysology and histopathology. Inter J Physiol Pathophysiol Pharmacol 2012; 4(1): 51-58.
26. Arha, D., Ramakrishna, E., Gupta, A. P., Rai, A. K., Sharma, A., Ahmad, I., Riyazuddin, M., Gayen, J. R., Maurya, R. and Tamrakar, A. K. Isoalantolactone derivative promotes glucose utilization in skeletal muscle cells and increases energy expenditure in db/db mice via activating AMPK-dependent signaling. Mol. Cellular Endocrinol. 2018; 460:134-151.
27. Kurek, K., Garbowska, M., Ziembicka, D. M., Łukaszuk, B., Rogowski, J., Chabowski, A., Górski, J. and Żendzian-Piotrowska, M. Myriocin treatment affects lipid metabolism in skeletal muscles of rats with streptozotocin-induced type 1 diabetes. Adv. Medical Sci., 2017; 62(1):65-73.
28. Al-Sharmaony, L., Al-Khazraji, S., Twaji, H. Hypoglycemic effect of a valuable extract on some blood glucose parameters in diabetic animals. J. Ethnopharmacol 2004; 43:167-171.
29. Aganbi, E., Avwioroko, O. J., Enabulele, E. R., Osagu, J. O., Uwandu, C. K., Ike, A., et al. Amelioration of lead-induced toxicity in blood, liver and kidney tissues of male wistar rats by fermented Ofada rice. Turkish J Agric - Food Sc Technol 2015; 3(9): 754-759.
30. Akah JA, Lemji JA, Salawa OA, Okoye TC, Offiah NV (2009). Effects of Vernonia amygdalina on Biochemical and Haematological Parameters in Diabetic Rats. Asian J Medicinal Sci 2009; 1(3): 108-113.
31. Parr, S. K., Matheny, M. E., Abdel-Kader, K., Greevy Jr, R. A., Bian, A., Fly, J., et al. Acute kidney injury is a risk factor for subsequent proteinuria. Kidney Inter 2017; 93(2): 460-469.
32. Bhargavi, G., Josthna, P., Naidu, CV. Changes in serum biochemical parameters and lipid profile in normal and STZ induced diabetic rats with the administration of ethanolic extract of Polpalthia cerasiodes stem bark. Intl. Res J. Pharm.2015; 6(2): 153-156.
33. Daisy, P., Nirmala, A., Rajathi, M. Hypoglycemic and other related effects of Elephantopus seaber extracts on alloxan-induced diabetic rats. J. Biol. Sci. 2007; 7:433-437.
34. Enogieru A. B., Momodu O. I., Omoruyi S. I., Om'iniabohs F. A. E. Changes in Biochemical Markers of Kidney Function and Antioxidant Status of Diabetic Rats treated with Aqueous Leaf Extracts of Ficus exasperata (Vahl). Afr J Biomed Res 2015; 18: 61- 67.
35. Pathak, A., Dhawan, D. Effects of lithium on the levels of blood urea and creatinine in diabetic rats. Med Sci Res 1998; 26:855-859
36. Ren, T., Zhu, Y., Xia, X., Ding, Y., Guo, J. and Kan, J.. Zanthoxylum alkylamides ameliorate protein metabolism disorder in STZ-induced diabetic rats. J Mol Endocrinol, 2017; 58(3): 113-125.
37. Edet, A. E., Patrick, E. B., Olarunfemi, E. A. Hematological Parameters of Alloxan-Induced Diabetic Rats Treated With Ethanol Extracts and Fractions of Naudea lafiloia Leaf. European Scientific J 2013; 9(27)203-10.
38. Kothari, R., Bokariya, P. A Comparative Study of Haematological Parameters in Type 1 Diabetes Mellitus Patients & Healthy Young Adolescents. Int J Biol Med Res 2012; 3(4): 2429-32.
39. Ashafa, A. O. T., Yakubu, M. T., Grierson, D. S., Afolayan, A. J. Toxicological Evaluation of the Aqueous Extract of Felicia muricata Thiemb Leaves in Wistar rats. Afri J Biolechnol 2009; 6(4): 949 – 954.
40. Tangvarasittichai, S. Serum levels of malondialdehyde in type 2 diabetes mellitus Thai subjects. Siriraj Medical J 2017; 61(1): 20-23.
41. Tsikas, D. Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges. Analytical biochemistry, 2017; 524:13-30.
42. Yin, L., Mano, J. I., Tanaka, K., Wang, S., Zhang, M., Deng, X., Zhang, S. High level of reduced glutathione contributes to detoxification of lipid peroxide-derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress. Physiologia plantarum, 2017; 161(2): 211-223.
43. Cho S. Y., Park J. Y., Park E. M., Choi M. S., Lee M. K., Jeon S. M., et al. Alternation of hepatic antioxidant enzyme activities and lipid profile in streptozotocin-induced diabetic rats by supplementation of dandelion water extract. Clin Chim Acta 2002; 317: 109-117.
44. Whitehead MW, Hawkes ND, Hainsworth I, Kingham, JG. A prospective study of the causes of notably raised aspartate aminotransferase of liver origin. Gut. 1999; 45:129-133.
45. Harris E. Elevated liver function tests in type 2 diabetes. Clin Diabetes 2005; 23:115-119.
46. Rajangam, U., Sampath, K., Thankaraj, S. M., Manoharan, R., Vasudevan, R. A., Sei, C. K., Andy, G., W. C. Hypoglycemic and Hypolipidaemic Effects of Withania somnifera Root and Leaf Extracts on Alloxan-Induced Diabetic Rats. Int. J. Mol. Sci 2009; 10: 2367-2382.