The Role of Ileum in the Absorption of Glucose in Normal and Alloxan-Induced Diabetic Rats Treated with Kolaviron

Olugbenga Adeola Odukanmi; Oyenike Lola Morakinyo; Tosan Peter Omayone; Samuel Babafemi Olaleye

doi:doi:10.11648/j.aap.20220702.11

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The Role of Ileum in the Absorption of Glucose in Normal and Alloxan-Induced Diabetic Rats Treated with Kolaviron

Olugbenga Adeola Odukanmi, Oyenike Lola Morakinyo, Tosan Peter Omayone, Samuel Babafemi Olaleye

Published in Advances in Applied Physiology (Volume 7, Issue 2)

Received: 17 July 2022 Accepted: 30 July 2022 Published: 10 August 2022

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Abstract

Kolaviron (KV) is an established antihyperglycemic agent in both normal and experimental diabetic rats. The role of ileum in glucose uptake in KV-treated normal and diabetic rats was investigated. Rats (150–180 g) were used and grouped into 4 (n=5/group). Groups 1 and 2 are the control (non-diabetic) and untreated diabetics respectively while groups 3 and 4 were treated with 200 mg/kg KV and 5mg/kg Glibenclamide (GB) orally respectively. Diabetes was induced by 120 mg/kg of alloxan. Before each phase, rats fasted for 12 h with free access to water. Phase 1 assessed the effect of treatments on blood glucose levels while Phases 2 and 3 investigated the route of glucose utilization following treatments. Rats were anaesthetized before laparotomy in Phases 2 and 3 following which 4 ml of modified Krebs solution was infused between 2 ligated ends of the ileum. Glucose concentration was determined by the glucose oxidase method. Data were analyzed and considered significant at P < 0.05. Fasting blood glucose of KV-treated and GB-treated decreased significantly compared to the Control and Diabetic after 2 h of KV administration. The A-V study recorded a 14.1%, 27.6%, and 19.3% decrease in A-V difference of blood glucose concentration in the untreated diabetics, KV-treated and GB-treated respectively compared to the control after 2 h. The ileum glucose absorption (mg/dl/cm) increased significantly in the KV-treated (12.67±1.2; 18.12±1.6) and GB treated (10.41±0.8; 16.51±1.1) when compared with the control (8.66±0.9; 11.61±1.6) and untreated diabetic (9.91±0.8; 11.13±0.9) after 90 and 120 minutes respectively. The mode of improved absorption into the enterocytes was not elucidated in this study. The role of the surviving pancreatic β-cells should be investigated.

Published in	Advances in Applied Physiology (Volume 7, Issue 2)
DOI	10.11648/j.aap.20220702.11
Page(s)	22-25
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Kolaviron, Ileum, Glucose Absorption, Rats, Antihyperglycemic, Diabetes

References

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[2]	Alada AR, Falokun PO, Oyebola DD. Intestinal glucose uptake in normal, untreated and insulin-treated diabetic dogs. Afr J Med Med Sci 2005; 34: 147–156.
[3]	Alada AR, Oyebola DD. The role of adrenergic receptors in the increased glucose uptake by the canine gut. Afr J Med Med Sci 1997; 26: 75–78.
[4]	Fujita Y, Kojima H, Hidaka H, et al. Increased intestinal glucose absorption and postprandial hyperglycaemia at the early step of glucose intolerance in Otsuka Long-Evans Tokushima Fatty rats. Diabetologia 1998; 41: 1459–1466.
[5]	Caspary WF. Increase of active transport of conjugated bile salts in streptozotocin-diabetic rat small intestine’. Gut 1973; 14: 949–955.
[6]	Csaky TZ, Fischer E. Intestinal sugar transport in experimental diabetes. Diabetes 1981; 30: 568–574.
[7]	Inzucchi SE. Oral antihyperglycemic therapy for type 2 diabetes: scientific review. JAMA 2002; 287: 360–372.
[8]	O’Keefe SJD, Buchman AL, Fishbein TM, et al. Short bowel syndrome and intestinal failure: consensus definitions and overview. Clin Gastroenterol Hepatol 2006; 4: 6–10.
[9]	Iqbal CW, Fatima J, Duenes J, et al. Expression and function of intestinal hexose transporters after small intestinal denervation. Surgery 2009; 146: 100–112.
[10]	Jurowich CF, Otto C, Rikkala PR, et al. Ileal Interposition in Rats with Experimental Type 2 Like Diabetes Improves Glycemic Control Independently of Glucose Absorption. J Diabetes Res; 2015. Epub ahead of print 2015. DOI: 10.1155/2015/490365.
[11]	Odukanmi OA., Oluwole FS. and Olaleye SB. Effects of Kolaviron, a Garcinia Kola biflavonoid, on Rat Intestinal Glucose Absorption and Alpha Amylase Inhibitory Activities. 2014.
[12]	Salau VF, Erukainure OL, Bharuth V, et al. Kolaviron stimulates glucose uptake with concomitant modulation of metabolic activities implicated in neurodegeneration in isolated rat brain, without perturbation of tissue ultrastructural morphology. Neurosci Res 2021; 169: 57–68.
[13]	Odukanmi OA, Salami AT, Ashaolu OP, et al. Kolaviron attenuates ischemia/reperfusion injury in the stomach of rats. Appl Physiol Nutr Metab 2018; 43: 33–37.
[14]	Salami A, Morakinyo O, Odukanmi O, et al. Kolaviron modulates intestinal motility and secretion in experimentally altered gut functions of Wistar rats. J Complement Med Res 2018; 9: 55–62.
[15]	Ajayi SA, Odukanmi OA, Ojo, GB. The Protective Effects of Kolaviron on the Renal Functions Journal of Pharmaceutical and Scientific Innovation THE PROTECTIVE EFFECTS OF KOLAVIRON ON THE RENAL FUNCTIONS OF FEMALE WISTAR RATS TREATED WITH CLOMIPHENE CITRATE. J Pharm Sci Innov 2012; 1: 102–107.
[16]	Iwu MM, Igboko OA, Elekwa OK, et al. Prevention of thioacetamide-induced hepatotoxicity by biflavanones of Garcinia kola. Phyther Res. Epub ahead of print 1990. DOI: 10.3/JQUERY-UI.JS.
[17]	Zheng Y, Sarr MG. Translocation of transfected GLUT2 to the apical membrane in rat intestinal IEC-6 cells. Dig Dis Sci 2011; 57: 1203–1212.
[18]	Kellett GL, Brot-Laroche E, Mace OJ, et al. Sugar absorption in the intestine: the role of GLUT2. Annu Rev Nutr 2008; 28: 35–54.
[19]	Rubin DC, Levin MS. Mechanisms of intestinal adaptation. Best Pract Res Clin Gastroenterol 2016; 30: 237–248.
[20]	Tappenden KA. Intestinal adaptation following resection. JPEN J Parenter Enteral Nutr 2014; 38: 23S-31S.

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Olugbenga Adeola Odukanmi, Oyenike Lola Morakinyo, Tosan Peter Omayone, Samuel Babafemi Olaleye. (2022). The Role of Ileum in the Absorption of Glucose in Normal and Alloxan-Induced Diabetic Rats Treated with Kolaviron. Advances in Applied Physiology, 7(2), 22-25. https://doi.org/10.11648/j.aap.20220702.11

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Olugbenga Adeola Odukanmi; Oyenike Lola Morakinyo; Tosan Peter Omayone; Samuel Babafemi Olaleye. The Role of Ileum in the Absorption of Glucose in Normal and Alloxan-Induced Diabetic Rats Treated with Kolaviron. Adv. Appl. Physiol. 2022, 7(2), 22-25. doi: 10.11648/j.aap.20220702.11

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AMA Style

Olugbenga Adeola Odukanmi, Oyenike Lola Morakinyo, Tosan Peter Omayone, Samuel Babafemi Olaleye. The Role of Ileum in the Absorption of Glucose in Normal and Alloxan-Induced Diabetic Rats Treated with Kolaviron. Adv Appl Physiol. 2022;7(2):22-25. doi: 10.11648/j.aap.20220702.11

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@article{10.11648/j.aap.20220702.11,
  author = {Olugbenga Adeola Odukanmi and Oyenike Lola Morakinyo and Tosan Peter Omayone and Samuel Babafemi Olaleye},
  title = {The Role of Ileum in the Absorption of Glucose in Normal and Alloxan-Induced Diabetic Rats Treated with Kolaviron},
  journal = {Advances in Applied Physiology},
  volume = {7},
  number = {2},
  pages = {22-25},
  doi = {10.11648/j.aap.20220702.11},
  url = {https://doi.org/10.11648/j.aap.20220702.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aap.20220702.11},
  abstract = {Kolaviron (KV) is an established antihyperglycemic agent in both normal and experimental diabetic rats. The role of ileum in glucose uptake in KV-treated normal and diabetic rats was investigated. Rats (150–180 g) were used and grouped into 4 (n=5/group). Groups 1 and 2 are the control (non-diabetic) and untreated diabetics respectively while groups 3 and 4 were treated with 200 mg/kg KV and 5mg/kg Glibenclamide (GB) orally respectively. Diabetes was induced by 120 mg/kg of alloxan. Before each phase, rats fasted for 12 h with free access to water. Phase 1 assessed the effect of treatments on blood glucose levels while Phases 2 and 3 investigated the route of glucose utilization following treatments. Rats were anaesthetized before laparotomy in Phases 2 and 3 following which 4 ml of modified Krebs solution was infused between 2 ligated ends of the ileum. Glucose concentration was determined by the glucose oxidase method. Data were analyzed and considered significant at P < 0.05. Fasting blood glucose of KV-treated and GB-treated decreased significantly compared to the Control and Diabetic after 2 h of KV administration. The A-V study recorded a 14.1%, 27.6%, and 19.3% decrease in A-V difference of blood glucose concentration in the untreated diabetics, KV-treated and GB-treated respectively compared to the control after 2 h. The ileum glucose absorption (mg/dl/cm) increased significantly in the KV-treated (12.67±1.2; 18.12±1.6) and GB treated (10.41±0.8; 16.51±1.1) when compared with the control (8.66±0.9; 11.61±1.6) and untreated diabetic (9.91±0.8; 11.13±0.9) after 90 and 120 minutes respectively. The mode of improved absorption into the enterocytes was not elucidated in this study. The role of the surviving pancreatic β-cells should be investigated.},
 year = {2022}
}

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TY - JOUR
T1 - The Role of Ileum in the Absorption of Glucose in Normal and Alloxan-Induced Diabetic Rats Treated with Kolaviron
AU - Olugbenga Adeola Odukanmi
AU - Oyenike Lola Morakinyo
AU - Tosan Peter Omayone
AU - Samuel Babafemi Olaleye
Y1 - 2022/08/10
PY - 2022
N1 - https://doi.org/10.11648/j.aap.20220702.11
DO - 10.11648/j.aap.20220702.11
T2 - Advances in Applied Physiology
JF - Advances in Applied Physiology
JO - Advances in Applied Physiology
SP - 22
EP - 25
PB - Science Publishing Group
SN - 2471-9714
UR - https://doi.org/10.11648/j.aap.20220702.11
AB - Kolaviron (KV) is an established antihyperglycemic agent in both normal and experimental diabetic rats. The role of ileum in glucose uptake in KV-treated normal and diabetic rats was investigated. Rats (150–180 g) were used and grouped into 4 (n=5/group). Groups 1 and 2 are the control (non-diabetic) and untreated diabetics respectively while groups 3 and 4 were treated with 200 mg/kg KV and 5mg/kg Glibenclamide (GB) orally respectively. Diabetes was induced by 120 mg/kg of alloxan. Before each phase, rats fasted for 12 h with free access to water. Phase 1 assessed the effect of treatments on blood glucose levels while Phases 2 and 3 investigated the route of glucose utilization following treatments. Rats were anaesthetized before laparotomy in Phases 2 and 3 following which 4 ml of modified Krebs solution was infused between 2 ligated ends of the ileum. Glucose concentration was determined by the glucose oxidase method. Data were analyzed and considered significant at P < 0.05. Fasting blood glucose of KV-treated and GB-treated decreased significantly compared to the Control and Diabetic after 2 h of KV administration. The A-V study recorded a 14.1%, 27.6%, and 19.3% decrease in A-V difference of blood glucose concentration in the untreated diabetics, KV-treated and GB-treated respectively compared to the control after 2 h. The ileum glucose absorption (mg/dl/cm) increased significantly in the KV-treated (12.67±1.2; 18.12±1.6) and GB treated (10.41±0.8; 16.51±1.1) when compared with the control (8.66±0.9; 11.61±1.6) and untreated diabetic (9.91±0.8; 11.13±0.9) after 90 and 120 minutes respectively. The mode of improved absorption into the enterocytes was not elucidated in this study. The role of the surviving pancreatic β-cells should be investigated.
VL - 7
IS - 2
ER -

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Author Information

Olugbenga Adeola Odukanmi

Laboratory for Gastrointestinal Secretion and Inflammation, Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria
Oyenike Lola Morakinyo

Laboratory for Gastrointestinal Secretion and Inflammation, Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria
Tosan Peter Omayone

Department of Physiology, Federal University of Technology, Akure, Nigeria
Samuel Babafemi Olaleye

Laboratory for Gastrointestinal Secretion and Inflammation, Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria

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APA Style

Olugbenga Adeola Odukanmi, Oyenike Lola Morakinyo, Tosan Peter Omayone, Samuel Babafemi Olaleye. (2022). The Role of Ileum in the Absorption of Glucose in Normal and Alloxan-Induced Diabetic Rats Treated with Kolaviron. Advances in Applied Physiology, 7(2), 22-25. https://doi.org/10.11648/j.aap.20220702.11

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Olugbenga Adeola Odukanmi; Oyenike Lola Morakinyo; Tosan Peter Omayone; Samuel Babafemi Olaleye. The Role of Ileum in the Absorption of Glucose in Normal and Alloxan-Induced Diabetic Rats Treated with Kolaviron. Adv. Appl. Physiol. 2022, 7(2), 22-25. doi: 10.11648/j.aap.20220702.11

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Olugbenga Adeola Odukanmi, Oyenike Lola Morakinyo, Tosan Peter Omayone, Samuel Babafemi Olaleye. The Role of Ileum in the Absorption of Glucose in Normal and Alloxan-Induced Diabetic Rats Treated with Kolaviron. Adv Appl Physiol. 2022;7(2):22-25. doi: 10.11648/j.aap.20220702.11

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@article{10.11648/j.aap.20220702.11,
  author = {Olugbenga Adeola Odukanmi and Oyenike Lola Morakinyo and Tosan Peter Omayone and Samuel Babafemi Olaleye},
  title = {The Role of Ileum in the Absorption of Glucose in Normal and Alloxan-Induced Diabetic Rats Treated with Kolaviron},
  journal = {Advances in Applied Physiology},
  volume = {7},
  number = {2},
  pages = {22-25},
  doi = {10.11648/j.aap.20220702.11},
  url = {https://doi.org/10.11648/j.aap.20220702.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aap.20220702.11},
  abstract = {Kolaviron (KV) is an established antihyperglycemic agent in both normal and experimental diabetic rats. The role of ileum in glucose uptake in KV-treated normal and diabetic rats was investigated. Rats (150–180 g) were used and grouped into 4 (n=5/group). Groups 1 and 2 are the control (non-diabetic) and untreated diabetics respectively while groups 3 and 4 were treated with 200 mg/kg KV and 5mg/kg Glibenclamide (GB) orally respectively. Diabetes was induced by 120 mg/kg of alloxan. Before each phase, rats fasted for 12 h with free access to water. Phase 1 assessed the effect of treatments on blood glucose levels while Phases 2 and 3 investigated the route of glucose utilization following treatments. Rats were anaesthetized before laparotomy in Phases 2 and 3 following which 4 ml of modified Krebs solution was infused between 2 ligated ends of the ileum. Glucose concentration was determined by the glucose oxidase method. Data were analyzed and considered significant at P < 0.05. Fasting blood glucose of KV-treated and GB-treated decreased significantly compared to the Control and Diabetic after 2 h of KV administration. The A-V study recorded a 14.1%, 27.6%, and 19.3% decrease in A-V difference of blood glucose concentration in the untreated diabetics, KV-treated and GB-treated respectively compared to the control after 2 h. The ileum glucose absorption (mg/dl/cm) increased significantly in the KV-treated (12.67±1.2; 18.12±1.6) and GB treated (10.41±0.8; 16.51±1.1) when compared with the control (8.66±0.9; 11.61±1.6) and untreated diabetic (9.91±0.8; 11.13±0.9) after 90 and 120 minutes respectively. The mode of improved absorption into the enterocytes was not elucidated in this study. The role of the surviving pancreatic β-cells should be investigated.},
 year = {2022}
}

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