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Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali

Received: 21 December 2021    Accepted: 10 January 2022    Published: 20 January 2022
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Abstract

Rabies are one of the major zoonoses in Mali. The control of this disease is based on the use of effective diagnostic techniques, the management of human cases after exposure and medical prophylaxis of animals. Diagnosis is usually made by detection of viral antigen by direct immunofluorescence (IF). Due to the increasing variability of the genus Lyssavirus observed, it was found necessary to use a complementary technique, namely reverse transcription in polymerase chain reaction (RT-PCR). The objective of the present study was to assess the diagnostic performance of IFD and RT-PCR for the diagnosis of animal rabies in Mali with a view to characterizing the different strains identified at the molecular level (gene of rabies virus nucleoprotein N). Thus, the analysis of forty (40) brain samples from dogs suspected of having rabies, revealed 36 positive cases for IFD, i.e. 90% and 33 for RT-PCR, i.e. 82.5.% and a concordance rate of 82.5%. Analysis of the segment of the gene of nucleoprotein (N) showed that the rabies viruses circulating in Mali belong to the Africa 2 lineage (Africa 2). Statistical analyzes showed the existence of a significant difference (P=0.001<0.005) between the performance of the two techniques. The present study shows the usefulness of the complementarity of the two tests as an alternative for confirming the diagnosis of rabies.

Published in American Journal of Biological and Environmental Statistics (Volume 8, Issue 1)
DOI 10.11648/j.ajbes.20220801.11
Page(s) 1-7
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Canine Rabies, Comparative Diagnosis, IFD, RT-PCR, Genotype

References
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[4] Boegel, K et Meslin, FX, 1990. Aspects économiques de l’élimination de la rage humaine et canine: principes pour l’orientation des programmes. Relation, 68 (4): 409–418.
[5] PARACON/GARC Global Alliance for Rabies Control, 2021.
[6] Dao, S., Abdillahi, A. M., Bougoudogo, F., Touré, K., Simbe, C., 2006. Aspects épidémiologiques de la rage humaine et animale dans la zone urbaine de Bamako, Mali. Bull. Soc. Pathol. Exot. 1990 (99), 183-186.
[7] OMS 2010; vaccins antirabiques. Note d'information de l'OMS: Relevé épidémiologique hebdomadaire; 32 (85); 309-320.
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[9] Deubelbeiss, A. M.-L. Zahno, M. Zanoni, D. Bruegger, and R. Zanoni 2014. Real-Time RT-PCR for the Detection of Lyssavirus Species Institute of Virology and Immunology, 3012 Berne, Switzerland.
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[18] Paola de Benedictis, Cristian de Battisti, Laurent Dacheux, Sabrina Marciano, Silvia Ormelli, et al, 2011. Lyssavirus detection and typing using pyrosequencing. Journal of Clinical Microbiology, American Society for Microbiology, 49 (5), pp. 1932–1938. 10.1128/JCM.02015-10. pasteur-01491354.
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Cite This Article
  • APA Style

    Sow Ibrahim, Dakouo Marthin, Coulibaly Kadidiatou, Diakité Mohamed Adama, Koné Yaya Sidi, et al. (2022). Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali. American Journal of Biological and Environmental Statistics, 8(1), 1-7. https://doi.org/10.11648/j.ajbes.20220801.11

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    ACS Style

    Sow Ibrahim; Dakouo Marthin; Coulibaly Kadidiatou; Diakité Mohamed Adama; Koné Yaya Sidi, et al. Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali. Am. J. Biol. Environ. Stat. 2022, 8(1), 1-7. doi: 10.11648/j.ajbes.20220801.11

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    AMA Style

    Sow Ibrahim, Dakouo Marthin, Coulibaly Kadidiatou, Diakité Mohamed Adama, Koné Yaya Sidi, et al. Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali. Am J Biol Environ Stat. 2022;8(1):1-7. doi: 10.11648/j.ajbes.20220801.11

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  • @article{10.11648/j.ajbes.20220801.11,
      author = {Sow Ibrahim and Dakouo Marthin and Coulibaly Kadidiatou and Diakité Mohamed Adama and Koné Yaya Sidi and Diakité Adama and Sidibé Satigui and Babana Amadou Hamadoun},
      title = {Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali},
      journal = {American Journal of Biological and Environmental Statistics},
      volume = {8},
      number = {1},
      pages = {1-7},
      doi = {10.11648/j.ajbes.20220801.11},
      url = {https://doi.org/10.11648/j.ajbes.20220801.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbes.20220801.11},
      abstract = {Rabies are one of the major zoonoses in Mali. The control of this disease is based on the use of effective diagnostic techniques, the management of human cases after exposure and medical prophylaxis of animals. Diagnosis is usually made by detection of viral antigen by direct immunofluorescence (IF). Due to the increasing variability of the genus Lyssavirus observed, it was found necessary to use a complementary technique, namely reverse transcription in polymerase chain reaction (RT-PCR). The objective of the present study was to assess the diagnostic performance of IFD and RT-PCR for the diagnosis of animal rabies in Mali with a view to characterizing the different strains identified at the molecular level (gene of rabies virus nucleoprotein N). Thus, the analysis of forty (40) brain samples from dogs suspected of having rabies, revealed 36 positive cases for IFD, i.e. 90% and 33 for RT-PCR, i.e. 82.5.% and a concordance rate of 82.5%. Analysis of the segment of the gene of nucleoprotein (N) showed that the rabies viruses circulating in Mali belong to the Africa 2 lineage (Africa 2). Statistical analyzes showed the existence of a significant difference (P=0.001<0.005) between the performance of the two techniques. The present study shows the usefulness of the complementarity of the two tests as an alternative for confirming the diagnosis of rabies.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali
    AU  - Sow Ibrahim
    AU  - Dakouo Marthin
    AU  - Coulibaly Kadidiatou
    AU  - Diakité Mohamed Adama
    AU  - Koné Yaya Sidi
    AU  - Diakité Adama
    AU  - Sidibé Satigui
    AU  - Babana Amadou Hamadoun
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    DO  - 10.11648/j.ajbes.20220801.11
    T2  - American Journal of Biological and Environmental Statistics
    JF  - American Journal of Biological and Environmental Statistics
    JO  - American Journal of Biological and Environmental Statistics
    SP  - 1
    EP  - 7
    PB  - Science Publishing Group
    SN  - 2471-979X
    UR  - https://doi.org/10.11648/j.ajbes.20220801.11
    AB  - Rabies are one of the major zoonoses in Mali. The control of this disease is based on the use of effective diagnostic techniques, the management of human cases after exposure and medical prophylaxis of animals. Diagnosis is usually made by detection of viral antigen by direct immunofluorescence (IF). Due to the increasing variability of the genus Lyssavirus observed, it was found necessary to use a complementary technique, namely reverse transcription in polymerase chain reaction (RT-PCR). The objective of the present study was to assess the diagnostic performance of IFD and RT-PCR for the diagnosis of animal rabies in Mali with a view to characterizing the different strains identified at the molecular level (gene of rabies virus nucleoprotein N). Thus, the analysis of forty (40) brain samples from dogs suspected of having rabies, revealed 36 positive cases for IFD, i.e. 90% and 33 for RT-PCR, i.e. 82.5.% and a concordance rate of 82.5%. Analysis of the segment of the gene of nucleoprotein (N) showed that the rabies viruses circulating in Mali belong to the Africa 2 lineage (Africa 2). Statistical analyzes showed the existence of a significant difference (P=0.001<0.005) between the performance of the two techniques. The present study shows the usefulness of the complementarity of the two tests as an alternative for confirming the diagnosis of rabies.
    VL  - 8
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Author Information
  • Departement of Biology, Faculty of Sciences & Techniques, University of Sciences, Bamako, Mali; Diagnosis and Research Service, Central Veterinary Laboratory, Bamako, Mali

  • Diagnosis and Research Service, Central Veterinary Laboratory, Bamako, Mali

  • Diagnosis and Research Service, Central Veterinary Laboratory, Bamako, Mali

  • Diagnosis and Research Service, Central Veterinary Laboratory, Bamako, Mali

  • Diagnosis and Research Service, Central Veterinary Laboratory, Bamako, Mali

  • Diagnosis and Research Service, Central Veterinary Laboratory, Bamako, Mali

  • Diagnosis and Research Service, Central Veterinary Laboratory, Bamako, Mali

  • Departement of Biology, Faculty of Sciences & Techniques, University of Sciences, Bamako, Mali

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