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The Effects of Gehuajiecheng Decoction on Expression of COX-2, Serum Level of PGE2, TNF-α and IL-6 in Alcoholic Hepatitis

Received: 22 April 2020     Accepted: 15 May 2020     Published: 29 May 2020
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Abstract

Background: In this experimental study, we have researched Gehuajiecheng decoction mechanism of action in the treatment of alcoholic hepatitis so as to supply an effective drug in clinical practice. Methods: 40 male Wistar rats were randomly divided into 4 groups and treated with different drugs. The control group were given intragastric administration of corn oil (2g/kg/per day) and 50% glucose (20ml/kg/per day); the model group were given intragastric administration of 40% alcohol (8g/kg/per day) and corn oil (2g/kg/per day); Gehuajiecheng decoction group were given intragastric administration of Gehuajiecheng decoction (13g/kg/per day) and 40% alcohol (8g/kg/per day); the Celecoxib group were given intragastric administration of celecoxib (10mg/kg/per day) and 40% alcohol (8g/kg/per day). 30 days after treatment, the rats were anesthetized with sodium pentobarbital and the blood was collected. Serum levels of AST, ALT and GGT were measured by using chromatometry, PGE2, TNF-α and IL-6 levels were examined by using radioimmunology method. After taking blood samples, the rats were sacrificed, and the liver was determined by Western blotting analysis. Results: Compared to controls, model group showed significantly higher levels of serum AST, ALT, GGT, PGE2, TNF-α and IL-6, which can be corrected by administration of Gehuajiecheng decoction or celecoxib, since the group treated with Gehuajiecheng decoction or celecoxib showed significantly lower levels of serum AST, ALT, GGT, PGE2, TNF-α and IL-6 (p<0.01). However, liver function and Inflammatory mediators in Gehuajiecheng decoction were not significantly lower than that of the celecoxib group (p>0.05). Similarly, COX-2 expression in the model group was significantly higher than that of controls. However, its expression can be depleted by Gehuajiecheng decoction or celecoxib as COX-2 expression in the model groups (p<0.01). Conclusion: Gehuajiecheng decoction protects against alcohol-induced liver injuries via inhibiting expression of COX-2, decreasing release of PGE2, TNF-α and IL-6. It has the same effect as COX-2 selective inhibitor, therefore, we suggest that this decoction could be used as an effective method in the treatment of alcoholic hepatitis.

Published in International Journal of Chinese Medicine (Volume 4, Issue 2)
DOI 10.11648/j.ijcm.20200402.12
Page(s) 21-26
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Gehuajiecheng Decoction, Alcoholic Hepatitis, Inflammatory Mediators, COX-2, Celecoxib

References
[1] Ashwani K Singal, Sudha Kodali, Lee A Vucovich, Victor Darley-Usmar, Thomas D Schiano Diagnosis and Treatment of Alcoholic Hepatitis: A Systematic Review. Alcohol Clin Exp Res. 2016; 40 (7): 1390–1402.
[2] Ling-Zu Kong, Nisansala Chandimali, Ying-Hao Han, Dong-Ho Lee, 4 Ji- Su Kim, 4 Sun-Uk Kim, Pathogenesis, Early Diagnosis, and Therapeutic Management of Alcoholic Liver Disease. Int J Mol Sci. 2019; 20 (11): 2712.
[3] Saggere Muralikrishna Shasthry, Shiv Kumar Sarin. New treatment options for alcoholic hepatitis. World J Gastroenterol. 2016; 22 (15): 3892–3906
[4] Fuster D, Samet JH. Alcohol Use in Patients with Chronic Liver Disease. N Engl J Med. 2018; 379 (13): 1251-1261.
[5] Sukhpreet Singh, Natalia A. O, Kusum K K. Treatment options for alcoholic and non-alcoholic fatty liver disease: A review. World J Gastroenterol. 2017; 23 (36): 6549–6570.
[6] S. Tome. Current management of alcoholic liver disease, Aliment Pharmacol Ther 2004; 19: 707-714.
[7] Li dongheng•jin, spleen and stomach, people's medical Press. China, 1st Ed., 2005: 81.
[8] Yao M, Liao Y, Li GQ, Law FC, Tang Y. Quantitative analysis of two isoflavones in Pueraria lobata flowers from eleven Chinese provinces using high performance liquid chromatography. Chin Med. 2010; 23 (5): 14.
[9] Zhang Z, Li S, Jiang J, Yu P, Liang J, Wang Y. Preventive effects of Flos Perariae (Gehua) water extract and its active ingredient puerarin in rodent alcoholism models, Chin Med. 2010; 5 (36): 2-8.
[10] Yang YY. Clinical observation of treatment of alcoholic fatty liver by Gehua Jiejiu Xiaozhi Decoction, Zhong Xi Yi Jie He Xue Bao. 2007; 5 (3): 343-5.
[11] Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice, J Integ Med 2015; 13 (4): 262-267.
[12] Feng R, Chen JH, Liu CH, Xia FB, Xiao Z, Zhang X, Wan JB. A combination of Pueraria lobata and Silybum marianum protects against alcoholic liver disease in mice, Phytomedicine. 2019; 58 (5): 152824.
[13] Ding RB, Tian K, Huang LL, He CW, Jiang Y, Wang YT, Wan JB. Herbal medicines for the prevention of alcoholic liver disease: a review. J Ethnopharmacol. 2012; 144 (3): 457-65.
[14] Mou HY, Nie HM, Hu XY. Gutuo Jiejiu decoction improves survival of patients with severe alcoholic hepatitis: A retrospective cohort study. World J Gastroenterol. 2017; 23 (16): 2957-2963.
[15] Wang S, Yang FJ, Shang LC, Zhang YH, Zhou Y, Shi XL. Puerarin protects against high-fat high-sucrose diet-induced non-alcoholic fatty liver disease by modulating PARP-1/PI3K/AKT signaling pathway and facilitating mitochondrial homeostasis. Phytother Res. 2019; 33 (9): 2347-2359.
[16] Yun Shao, Kun Sun, Wei Xu, Xiao-Lin Li, Hong Shen, Wei-Hao Sun. Helicobacter pylori infection, gastrin and cyclooxygenase-2 in gastric carcinogenesis. World J Gastroenterol. 2014; 20 (36): 12860–12873.
[17] Raimundo F. d. A. J., Vinícius B. G., Renata F. d. C. L., Gerly A. d. C. B., Emilio d. C. M., Paulo M. M. Carvedilol Improves Inflammatory Response, Oxidative Stress and Fibrosis in the Alcohol-Induced Liver Injury in Rats by Regulating Kuppfer Cells and Hepatic Stellate Cells. PLoS One. 2016; 11 (2): e0148868.
[18] Jorge Castro-López, Antonio Ramis, Marta Planellas, Mariana Teles, Josep Pastor. Cyclooxygenase-2 immunoexpression in intestinal epithelium and lamina propria of cats with inflammatory bowel disease and low grade alimentary lymphoma. BMC Vet Res. 2018; 14: 158.
[19] Bykov L, Palmen M, Rainsford K. D, Lindros K. O. Chronic effects of celecoxib, a cyclooxygenase-2 inhibitor, cause enhanced alcohol-induced liver steatosis in rats. Inflammopharmacology. 2006; 14: 36-41.
[20] Senthilkumar R, Nalini N., Effect of Glycineon tissue Fatty Acid Composition in an exprimental Model of Alcohol-Induced Hepatotoxity. Clin Exp Pharmacol Physiol. 2004, 31: 456-461.
[21] Saravanan N, Nalini N. Antioxidant effect of Hemidesmus indicus on ethanol-induced hepatotoxicity in rats. J Med Food. 2007 Dec; 10 (4): 675-82.
[22] Tipoe GL, Liong EC, Casey CA, Donohue TM Jr, Eagon PK, So H, et al. A voluntary oral ethanol-feeding rat model associated with necroinflammatory liver injury. Alcohol Clin Exp Res. 2008; 32 (4): 669-82.
[23] Irie M, Suzuki N, Sohda T, Anan A, Iwata K, Takeyama Y, Watanabe H, Fischer P, Scherberich JE, Sakisaka S Hepatic expression of gamma-glutamyltranspeptidase in the human liver of patients with alcoholic liver disease. Hepatol Res. 2007; 37: 966-73.
[24] Elisabetta Ceni, Tommaso Mello, Andrea Galli. Pathogenesis of alcoholic liver disease: Role of oxidative metabolism World J Gastroenterol. 2014; 20 (47): 17756–17772.
[25] Arulkumar Nagappan, Dae Young Jung, Ji-Hyun Kim, Hoyoung Lee, Myeong Ho Jung. Gomisin N Alleviates Ethanol-Induced Liver Injury through Ameliorating Lipid Metabolism and Oxidative Stress. Int J Mol Sci. 2018; 19 (9): 2601.
[26] QQ. Dong, F Chu, CZ Wu, Q Huo, HY Gan, XM Li, H Liu. Scutellaria baicalensis Georgi extract protects against alcohol-induced acute liver injury in mice and affects the mechanism of ER stress. Mol Med Rep. 2016; 13 (4): 3052–3062.
[27] Sougioultzis S, Dalakas E, Hayes PC, Plevris JN. Alcoholic hepatitis: from pathogenesis to treatment. Curr Med Res Opin 2005; 21 (9): 1337-46.
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    Zhiwei Qu, Wenbo Ju, Aihua Ren, Maoyang Liu. (2020). The Effects of Gehuajiecheng Decoction on Expression of COX-2, Serum Level of PGE2, TNF-α and IL-6 in Alcoholic Hepatitis. International Journal of Chinese Medicine, 4(2), 21-26. https://doi.org/10.11648/j.ijcm.20200402.12

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    Zhiwei Qu; Wenbo Ju; Aihua Ren; Maoyang Liu. The Effects of Gehuajiecheng Decoction on Expression of COX-2, Serum Level of PGE2, TNF-α and IL-6 in Alcoholic Hepatitis. Int. J. Chin. Med. 2020, 4(2), 21-26. doi: 10.11648/j.ijcm.20200402.12

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    AMA Style

    Zhiwei Qu, Wenbo Ju, Aihua Ren, Maoyang Liu. The Effects of Gehuajiecheng Decoction on Expression of COX-2, Serum Level of PGE2, TNF-α and IL-6 in Alcoholic Hepatitis. Int J Chin Med. 2020;4(2):21-26. doi: 10.11648/j.ijcm.20200402.12

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  • @article{10.11648/j.ijcm.20200402.12,
      author = {Zhiwei Qu and Wenbo Ju and Aihua Ren and Maoyang Liu},
      title = {The Effects of Gehuajiecheng Decoction on Expression of COX-2, Serum Level of PGE2, TNF-α and IL-6 in Alcoholic Hepatitis},
      journal = {International Journal of Chinese Medicine},
      volume = {4},
      number = {2},
      pages = {21-26},
      doi = {10.11648/j.ijcm.20200402.12},
      url = {https://doi.org/10.11648/j.ijcm.20200402.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcm.20200402.12},
      abstract = {Background: In this experimental study, we have researched Gehuajiecheng decoction mechanism of action in the treatment of alcoholic hepatitis so as to supply an effective drug in clinical practice. Methods: 40 male Wistar rats were randomly divided into 4 groups and treated with different drugs. The control group were given intragastric administration of corn oil (2g/kg/per day) and 50% glucose (20ml/kg/per day); the model group were given intragastric administration of 40% alcohol (8g/kg/per day) and corn oil (2g/kg/per day); Gehuajiecheng decoction group were given intragastric administration of Gehuajiecheng decoction (13g/kg/per day) and 40% alcohol (8g/kg/per day); the Celecoxib group were given intragastric administration of celecoxib (10mg/kg/per day) and 40% alcohol (8g/kg/per day). 30 days after treatment, the rats were anesthetized with sodium pentobarbital and the blood was collected. Serum levels of AST, ALT and GGT were measured by using chromatometry, PGE2, TNF-α and IL-6 levels were examined by using radioimmunology method. After taking blood samples, the rats were sacrificed, and the liver was determined by Western blotting analysis. Results: Compared to controls, model group showed significantly higher levels of serum AST, ALT, GGT, PGE2, TNF-α and IL-6, which can be corrected by administration of Gehuajiecheng decoction or celecoxib, since the group treated with Gehuajiecheng decoction or celecoxib showed significantly lower levels of serum AST, ALT, GGT, PGE2, TNF-α and IL-6 (p0.05). Similarly, COX-2 expression in the model group was significantly higher than that of controls. However, its expression can be depleted by Gehuajiecheng decoction or celecoxib as COX-2 expression in the model groups (p<0.01). Conclusion: Gehuajiecheng decoction protects against alcohol-induced liver injuries via inhibiting expression of COX-2, decreasing release of PGE2, TNF-α and IL-6. It has the same effect as COX-2 selective inhibitor, therefore, we suggest that this decoction could be used as an effective method in the treatment of alcoholic hepatitis.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - The Effects of Gehuajiecheng Decoction on Expression of COX-2, Serum Level of PGE2, TNF-α and IL-6 in Alcoholic Hepatitis
    AU  - Zhiwei Qu
    AU  - Wenbo Ju
    AU  - Aihua Ren
    AU  - Maoyang Liu
    Y1  - 2020/05/29
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ijcm.20200402.12
    DO  - 10.11648/j.ijcm.20200402.12
    T2  - International Journal of Chinese Medicine
    JF  - International Journal of Chinese Medicine
    JO  - International Journal of Chinese Medicine
    SP  - 21
    EP  - 26
    PB  - Science Publishing Group
    SN  - 2578-9473
    UR  - https://doi.org/10.11648/j.ijcm.20200402.12
    AB  - Background: In this experimental study, we have researched Gehuajiecheng decoction mechanism of action in the treatment of alcoholic hepatitis so as to supply an effective drug in clinical practice. Methods: 40 male Wistar rats were randomly divided into 4 groups and treated with different drugs. The control group were given intragastric administration of corn oil (2g/kg/per day) and 50% glucose (20ml/kg/per day); the model group were given intragastric administration of 40% alcohol (8g/kg/per day) and corn oil (2g/kg/per day); Gehuajiecheng decoction group were given intragastric administration of Gehuajiecheng decoction (13g/kg/per day) and 40% alcohol (8g/kg/per day); the Celecoxib group were given intragastric administration of celecoxib (10mg/kg/per day) and 40% alcohol (8g/kg/per day). 30 days after treatment, the rats were anesthetized with sodium pentobarbital and the blood was collected. Serum levels of AST, ALT and GGT were measured by using chromatometry, PGE2, TNF-α and IL-6 levels were examined by using radioimmunology method. After taking blood samples, the rats were sacrificed, and the liver was determined by Western blotting analysis. Results: Compared to controls, model group showed significantly higher levels of serum AST, ALT, GGT, PGE2, TNF-α and IL-6, which can be corrected by administration of Gehuajiecheng decoction or celecoxib, since the group treated with Gehuajiecheng decoction or celecoxib showed significantly lower levels of serum AST, ALT, GGT, PGE2, TNF-α and IL-6 (p0.05). Similarly, COX-2 expression in the model group was significantly higher than that of controls. However, its expression can be depleted by Gehuajiecheng decoction or celecoxib as COX-2 expression in the model groups (p<0.01). Conclusion: Gehuajiecheng decoction protects against alcohol-induced liver injuries via inhibiting expression of COX-2, decreasing release of PGE2, TNF-α and IL-6. It has the same effect as COX-2 selective inhibitor, therefore, we suggest that this decoction could be used as an effective method in the treatment of alcoholic hepatitis.
    VL  - 4
    IS  - 2
    ER  - 

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Author Information
  • Department of Traditional Chinese Medicine, The Affiliated Hospital of Beihua University, Jilin City, China

  • Department of Anatomy, Beihua University, Jilin City, China

  • Department of Anatomy, Beihua University, Jilin City, China

  • Department of Forensic Medicine, Beihua University, Jilin City, China

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