| Peer-Reviewed

HPLC Method for the Determination Content of 5-HMF in Zishen Yutai Pill

Received: 26 February 2021    Accepted: 17 March 2021    Published: 26 March 2021
Views:       Downloads:
Abstract

Objective: During the preparation of Zishen Yutai Pills, 5-Hydroxymethylfurfural may be used. To establish a HPLC method for determination of 5-HMF in Zishen Yutai pill and to provide the potential quality control standard of it. Methods: RP-HPLC method was applied with the chromatographic condition as follows: the chromatographic column was Phenomenex Gemini C18 column (250×4.6mm, 5μm), methanol-water (10:90) as the mobile phrase, the flow rate was 0.8mL/min, the detection wavelength was 284nm, the column temperature was 30°C, and the injection volume was 10μL. Results: The calibration curve was linear in the range of 2.16~43.27μg. mL-1 for 5-HMF (r2=0.9993). The RSD of the method repeatability was 0.97% (n=6) and the average recovery was 99.4% (RSD=0.58%). What’s more, the specificity, precision and stability results were also good. Conclusion: The method for HPLC is not only sensitive and accurate. It is also easy to operate which can be used as the determination method for 5-HMF and has a guiding role for the quality control of the Zishen Yutai pill.

Published in International Journal of Chinese Medicine (Volume 5, Issue 1)
DOI 10.11648/j.ijcm.20210501.13
Page(s) 14-17
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

HPLC, Zishen Yutai Pill, 5-HMF, Determination, Methodology

References
[1] Ying S and Fengjuan H. (2020). Mechanisms of Zishen Yutai Pills Intervention with Kidney Yang as Mediator in the Treatment of Diminished Ovarian Reserve. Jilin Journal of Chinese Medicine 40: 1117-1120.
[2] Mei W, Yuexin W, Yingru W, Ning L, Min F, Wenzhi Z, Ran J, Xuliang H and Yan N. (2020). Research progress on content changes of 5-hydroxymethyl furfural during processing of Chinese medicine and it's pharmacological effects. Drug Evaluation Research 43: 2319-2327.
[3] Juncheng W. (2020). Determination of 5-hydroxymethyl Furfural in Breviscapine and Glucose Injection by HPLC and Its Limit Setting. Asia-Pacific Traditional Medicine 16: 47-50.
[4] Ling L and Naijiang C. (2020). Determination of Genotoxic Impurity 5-Hydroxymethylfurfural in Calcium Gluconate Oral Solution by HPLC. China Pharmacist 23: 360-362.
[5] Fu-Hai S. (2020). Preparation of 5-hydroxymethylfurfural in honey. Journal of Food Safety & Quality 11: 6450-6455.
[6] Kong F, Lee BH and Wei K. (2019). 5-Hydroxymethylfurfural Mitigates Lipopolysaccharide-Stimulated Inflammation via Suppression of MAPK, NF-κB and mTOR Activation in RAW 264.7 Cells. Molecules 24: 275.
[7] Jia L, Yichao L, Yang Y and Feng-juan H. (2019). Clinical observation of the effect of zishenyufeteng pill on endometrial volume of IVF-ET patients with repeated failure of kidney deficiency. Journal of Tianjin University of Traditional Chinese Medicine 38.
[8] Qi-guo HYYL. (2019). Study on the 5-hydroxymethylfurfural Constituents of Jiu Hua Rhizoma Polygnoati in"Nine-steam-nine-bask"Processing. Journal of Dezhou University: 29-32.
[9] Anburajan P, Pugazhendhi A, Park J, Sivagurunathan P, Kumar G and Kim S. (2018). Effect of 5-hydroxymethylfurfural (5-HMF) on high-rate continuous biohydrogen production from galactose. Bioresour Technol: 1197-1200.
[10] Lin L, Ni L, Yahao L, Encan L, Xiaoyu F, Sheng L, Qingce Z, Jiuming H and Hongtao J. (2018). Acute toxicity and cytotoxicity of 5-hydroxymethyl furfural.: 205-209.
[11] Zhuo Pan, He-Xin Shao, Liu T, Lu X and Fan X. (2017). Research progress of 5-hydroxymethylfurfural, a safety-related substance in traditional Chinese medicine injections. Zhongguo Zhong Yao Za Zhi 42: 1842-1846.
[12] Tingfeng W, Yuantang X and Danru Z. (2017). Efficacy and Safety Analysis of Zishen Yutai Pills Combined with Progesterone in the Treatment of Threatened Abortion. Journal of Hunan University of Chinese Medicine 39: 907-912.
[13] Zhou L, Huang Q, Wang R, Zhou J, Ma A, Chong L, Wu Y, Wang Y, Xu L, Chen Y, Jia Y, Gui B and Sun Z. (2016). Reproductive Toxicity of Zishen Yutai Pill in Rats: The Fertility and Early Embryonic Development Study (Segment I). Evid Based Complement Alternat Med 2016: 3175902.
[14] Ke-Han P and Shi-Yi O. (2016). Formation and mitigation of 5-hydroxymethylfurfural in foods. Journal of Food Safety & Quality 7: 251-256.
[15] Gao Q, Han L, Li X, Cai X and Li X. (2015). Traditional Chinese Medicine, the Zishen Yutai Pill, Ameliorates Precocious Endometrial Maturation Induced by Controlled Ovarian Hyperstimulation and Improves Uterine Receptivity via Upregulation of HOXA10. Evidence-based complementary and alternative medicine 2015: 317510-317586.
Cite This Article
  • APA Style

    Lin Shaozhu, Huang Minna, Liu Hui, Huang Jiawen, Jin Weijun, et al. (2021). HPLC Method for the Determination Content of 5-HMF in Zishen Yutai Pill. International Journal of Chinese Medicine, 5(1), 14-17. https://doi.org/10.11648/j.ijcm.20210501.13

    Copy | Download

    ACS Style

    Lin Shaozhu; Huang Minna; Liu Hui; Huang Jiawen; Jin Weijun, et al. HPLC Method for the Determination Content of 5-HMF in Zishen Yutai Pill. Int. J. Chin. Med. 2021, 5(1), 14-17. doi: 10.11648/j.ijcm.20210501.13

    Copy | Download

    AMA Style

    Lin Shaozhu, Huang Minna, Liu Hui, Huang Jiawen, Jin Weijun, et al. HPLC Method for the Determination Content of 5-HMF in Zishen Yutai Pill. Int J Chin Med. 2021;5(1):14-17. doi: 10.11648/j.ijcm.20210501.13

    Copy | Download

  • @article{10.11648/j.ijcm.20210501.13,
      author = {Lin Shaozhu and Huang Minna and Liu Hui and Huang Jiawen and Jin Weijun and Wu Xiaosong},
      title = {HPLC Method for the Determination Content of 5-HMF in Zishen Yutai Pill},
      journal = {International Journal of Chinese Medicine},
      volume = {5},
      number = {1},
      pages = {14-17},
      doi = {10.11648/j.ijcm.20210501.13},
      url = {https://doi.org/10.11648/j.ijcm.20210501.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcm.20210501.13},
      abstract = {Objective: During the preparation of Zishen Yutai Pills, 5-Hydroxymethylfurfural may be used. To establish a HPLC method for determination of 5-HMF in Zishen Yutai pill and to provide the potential quality control standard of it. Methods: RP-HPLC method was applied with the chromatographic condition as follows: the chromatographic column was Phenomenex Gemini C18 column (250×4.6mm, 5μm), methanol-water (10:90) as the mobile phrase, the flow rate was 0.8mL/min, the detection wavelength was 284nm, the column temperature was 30°C, and the injection volume was 10μL. Results: The calibration curve was linear in the range of 2.16~43.27μg. mL-1 for 5-HMF (r2=0.9993). The RSD of the method repeatability was 0.97% (n=6) and the average recovery was 99.4% (RSD=0.58%). What’s more, the specificity, precision and stability results were also good. Conclusion: The method for HPLC is not only sensitive and accurate. It is also easy to operate which can be used as the determination method for 5-HMF and has a guiding role for the quality control of the Zishen Yutai pill.},
     year = {2021}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - HPLC Method for the Determination Content of 5-HMF in Zishen Yutai Pill
    AU  - Lin Shaozhu
    AU  - Huang Minna
    AU  - Liu Hui
    AU  - Huang Jiawen
    AU  - Jin Weijun
    AU  - Wu Xiaosong
    Y1  - 2021/03/26
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ijcm.20210501.13
    DO  - 10.11648/j.ijcm.20210501.13
    T2  - International Journal of Chinese Medicine
    JF  - International Journal of Chinese Medicine
    JO  - International Journal of Chinese Medicine
    SP  - 14
    EP  - 17
    PB  - Science Publishing Group
    SN  - 2578-9473
    UR  - https://doi.org/10.11648/j.ijcm.20210501.13
    AB  - Objective: During the preparation of Zishen Yutai Pills, 5-Hydroxymethylfurfural may be used. To establish a HPLC method for determination of 5-HMF in Zishen Yutai pill and to provide the potential quality control standard of it. Methods: RP-HPLC method was applied with the chromatographic condition as follows: the chromatographic column was Phenomenex Gemini C18 column (250×4.6mm, 5μm), methanol-water (10:90) as the mobile phrase, the flow rate was 0.8mL/min, the detection wavelength was 284nm, the column temperature was 30°C, and the injection volume was 10μL. Results: The calibration curve was linear in the range of 2.16~43.27μg. mL-1 for 5-HMF (r2=0.9993). The RSD of the method repeatability was 0.97% (n=6) and the average recovery was 99.4% (RSD=0.58%). What’s more, the specificity, precision and stability results were also good. Conclusion: The method for HPLC is not only sensitive and accurate. It is also easy to operate which can be used as the determination method for 5-HMF and has a guiding role for the quality control of the Zishen Yutai pill.
    VL  - 5
    IS  - 1
    ER  - 

    Copy | Download

Author Information
  • Department of Pharmacy, The First Affiliated Hospital of Jinan University, Guangzhou, China

  • Department of Pharmacy, The First Affiliated Hospital of Jinan University, Guangzhou, China

  • Department of Pharmacy, The First Affiliated Hospital of Jinan University, Guangzhou, China

  • Department of Pharmacy, The First Affiliated Hospital of Jinan University, Guangzhou, China

  • Department of Pharmacy, The First Affiliated Hospital of Jinan University, Guangzhou, China

  • Department of Pharmacy, The First Affiliated Hospital of Jinan University, Guangzhou, China

  • Sections