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Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment

Received: 1 October 2015     Accepted: 13 October 2015     Published: 16 November 2015
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Abstract

Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Trivedi’s biofield treatment on lyophilized as well as revived state of P. vulgaris for antimicrobial susceptibility pattern, biochemical characteristics, and biotype. P. vulgaris cells were procured from Micro BioLogics Inc., USA, in sealed pack bearing the American Type Culture Collection (ATCC 33420) number and stored according to the recommended storage protocol until needed for experiments. Lyophilized vial of ATCC strain of P. vulgaris were divided in two parts, Gr. I: control and Gr. II: treatment. Group II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10. Gr. IIB was stored and analysed on day 143. After retreatment on day 143, the sample was divided into three separate tubes. First, second and third tubes were analysed on day 5, 10 and 15 respectively. All experimental parameters were studied using automated Micro Scan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of P. vulgaris with other bacterial species after treatment. The antimicrobial susceptibility and minimum inhibitory concentration showed 10.71% and 15.63% alteration respectively in treated cells of P. vulgaris as compared to control. It was observed that few biochemical reactions (6%) were altered in the treated groups with respect to control. Moreover, biotype number was substantially changed in treated cells, Gr. IIA (62060406, Proteus penneri) on day 10 as compared to control (62070406; Proteus vulgaris). 16S rDNA analysis showed that the identified sample in this experiment was Proteus vulgaris after biofield treatment. However, the nearest homolog genus-species was found to be Proteus hauseri. The results suggested that biofield treatment has impact on P. vulgaris in lyophilized as well as revived state.

Published in International Journal of Genetics and Genomics (Volume 3, Issue 6)
DOI 10.11648/j.ijgg.20150306.12
Page(s) 66-73
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Proteus vulgaris, Antimicrobial Susceptibility, Biofield Treatment, Biochemical Reaction, Biotype, 16S rDNA Analysis

References
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[2] Herter CA, Broeck CT (1911) A biochemical study of Proteus vulgaris Hauser. J Biol Chem 9: 491-511.
[3] Ghaidaa M, Yanchang W, Abdallah H (2013) The effect of p-nitrophenylglycerol on swarming and the production of some virulence factors in Proteus vulgaris. N Y Sci J 6: 8-14.
[4] O'Hara CM, Brenner FW, Miller JM (2000) Classification, identification, and clinical significance of Proteus, Providencia, and Morganella. Clin Microbiol Rev 13: 534-546.
[5] Burr HS (1957) Bibliography of Harold Saxton Burr. Yale J Biol Med 30: 163-167.
[6] Hammerschlag R, Jain S, Baldwin AL, Gronowicz G, Lutgendorf SK, et al. (2012) Biofield research: A roundtable discussion of scientific and methodological issues. J Altern Complement Med 18: 1081-1086.
[7] Movaffaghi Z, Farsi M (2009) Biofield therapies: Biophysical basis and biological regulations? Complement Ther Clin Pract 15: 35-37.
[8] Rivera-Ruiz M, Cajavilca C, Varon J (2008) Einthoven's string galvanometer: The first electrocardiograph. Tex Heart Inst J 35: 174-178.
[9] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O (2015) Studies of the atomic and crystalline characteristics of ceramic oxide nano powders after bio field treatment. Ind Eng Manage 4: 161.
[10] Dabhade VV, Tallapragada RR, Trivedi MK (2009) Effect of external energy on atomic, crystalline and powder characteristics of antimony and bismuth powders. Bull Mater Sci 32: 471-479.
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[16] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Antimicrobial sensitivity pattern of Pseudomonas fluorescens after biofield treatment. J Infect Dis Ther 3: 222.
[17] Patil SA, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of biofield treatment on growth and anatomical characteristics of Pogostemon cablin (Benth). Biotechnology 11: 154-162.
[18] Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.
[19] Fader RC, Weaver E, Fossett R, Toyras M, Vanderlaan J, et al. (2013) Multilaboratory study of the biomic automated well-reading instrument versus Micro Scan Walk Away for reading MicroScan antimicrobial susceptibility and identification panels. J Clin Microbiol 51: 1548-1554.
[20] Kumar S, Tamura K, Nei, M (2004) MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5: 150-163.
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Cite This Article
  • APA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, et al. (2015). Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment. International Journal of Genetics and Genomics, 3(6), 66-73. https://doi.org/10.11648/j.ijgg.20150306.12

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    ACS Style

    Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Sambhu Charan Mondal, et al. Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment. Int. J. Genet. Genomics 2015, 3(6), 66-73. doi: 10.11648/j.ijgg.20150306.12

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    AMA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, et al. Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment. Int J Genet Genomics. 2015;3(6):66-73. doi: 10.11648/j.ijgg.20150306.12

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  • @article{10.11648/j.ijgg.20150306.12,
      author = {Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Sambhu Charan Mondal and Snehasis Jana},
      title = {Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment},
      journal = {International Journal of Genetics and Genomics},
      volume = {3},
      number = {6},
      pages = {66-73},
      doi = {10.11648/j.ijgg.20150306.12},
      url = {https://doi.org/10.11648/j.ijgg.20150306.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20150306.12},
      abstract = {Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Trivedi’s biofield treatment on lyophilized as well as revived state of P. vulgaris for antimicrobial susceptibility pattern, biochemical characteristics, and biotype. P. vulgaris cells were procured from Micro BioLogics Inc., USA, in sealed pack bearing the American Type Culture Collection (ATCC 33420) number and stored according to the recommended storage protocol until needed for experiments. Lyophilized vial of ATCC strain of P. vulgaris were divided in two parts, Gr. I: control and Gr. II: treatment. Group II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10. Gr. IIB was stored and analysed on day 143. After retreatment on day 143, the sample was divided into three separate tubes. First, second and third tubes were analysed on day 5, 10 and 15 respectively. All experimental parameters were studied using automated Micro Scan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of P. vulgaris with other bacterial species after treatment. The antimicrobial susceptibility and minimum inhibitory concentration showed 10.71% and 15.63% alteration respectively in treated cells of P. vulgaris as compared to control. It was observed that few biochemical reactions (6%) were altered in the treated groups with respect to control. Moreover, biotype number was substantially changed in treated cells, Gr. IIA (62060406, Proteus penneri) on day 10 as compared to control (62070406; Proteus vulgaris). 16S rDNA analysis showed that the identified sample in this experiment was Proteus vulgaris after biofield treatment. However, the nearest homolog genus-species was found to be Proteus hauseri. The results suggested that biofield treatment has impact on P. vulgaris in lyophilized as well as revived state.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment
    AU  - Mahendra Kumar Trivedi
    AU  - Alice Branton
    AU  - Dahryn Trivedi
    AU  - Gopal Nayak
    AU  - Sambhu Charan Mondal
    AU  - Snehasis Jana
    Y1  - 2015/11/16
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ijgg.20150306.12
    DO  - 10.11648/j.ijgg.20150306.12
    T2  - International Journal of Genetics and Genomics
    JF  - International Journal of Genetics and Genomics
    JO  - International Journal of Genetics and Genomics
    SP  - 66
    EP  - 73
    PB  - Science Publishing Group
    SN  - 2376-7359
    UR  - https://doi.org/10.11648/j.ijgg.20150306.12
    AB  - Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Trivedi’s biofield treatment on lyophilized as well as revived state of P. vulgaris for antimicrobial susceptibility pattern, biochemical characteristics, and biotype. P. vulgaris cells were procured from Micro BioLogics Inc., USA, in sealed pack bearing the American Type Culture Collection (ATCC 33420) number and stored according to the recommended storage protocol until needed for experiments. Lyophilized vial of ATCC strain of P. vulgaris were divided in two parts, Gr. I: control and Gr. II: treatment. Group II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10. Gr. IIB was stored and analysed on day 143. After retreatment on day 143, the sample was divided into three separate tubes. First, second and third tubes were analysed on day 5, 10 and 15 respectively. All experimental parameters were studied using automated Micro Scan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of P. vulgaris with other bacterial species after treatment. The antimicrobial susceptibility and minimum inhibitory concentration showed 10.71% and 15.63% alteration respectively in treated cells of P. vulgaris as compared to control. It was observed that few biochemical reactions (6%) were altered in the treated groups with respect to control. Moreover, biotype number was substantially changed in treated cells, Gr. IIA (62060406, Proteus penneri) on day 10 as compared to control (62070406; Proteus vulgaris). 16S rDNA analysis showed that the identified sample in this experiment was Proteus vulgaris after biofield treatment. However, the nearest homolog genus-species was found to be Proteus hauseri. The results suggested that biofield treatment has impact on P. vulgaris in lyophilized as well as revived state.
    VL  - 3
    IS  - 6
    ER  - 

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Author Information
  • Trivedi Global Inc., Henderson, USA

  • Trivedi Global Inc., Henderson, USA

  • Trivedi Global Inc., Henderson, USA

  • Trivedi Global Inc., Henderson, USA

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

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